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Citations for Thermo Fisher :
1551 - 1600 of 5822 citations for ZBED1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher) at a 1:1 bead to cell ratio ...
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bioRxiv - Immunology 2024Quote: ... with anti-human CD107a-eFluor660 (clone H4A3, Invitrogen, UK) at an E:T ratio of 1:1 for a total of 4 hours at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... Human neonatal epidermal keratinocytes (HEKn, Thermo Fisher Scientific, C0015C) were cultured in EpiLife Medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Human neonatal dermal fibroblasts (HDFn, Thermo Fisher Scientific, C0045C) were cultured in Medium 106 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Coding sequence for sbp2 was PCR amplified from 0-5 dpf pooled cDNA using the forward and reverse primers shown in Table 1 and then TA cloned into pcDNA3.1 V5/His Topo (Thermo Fisher Scientific). For secisbp2l ...
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bioRxiv - Molecular Biology 2021Quote: ... biotin labelled Hi-C 3.0 DNAs were purified by 20 μl Dynabeads® MyOne™ Streptavidin C1 beads (Thermo Fisher 65002). The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1.5ml of SD/-Leu/-His liquid medium (plus 3 mM 3-AT) were incubated in a 12-well microplate (Thermo Scientific) at 30°C for 96 hours (h) ...
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bioRxiv - Molecular Biology 2020Quote: Approximately 4 x 105 HEK293T cells in a 6-well plate were transfected with 250 ng/well of pMT107-octa-6 His-ubiquitin or 200 ng/well of pcDNA3-2x-6His-ubiquitin constructs using 50 µl/well Opti-MEM (Gibco; 31985062) and 2 µl/well of FuGENE 6 Transfection reagent (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant baculovirus containing the sequence of ΔPRD-dynamin 1 with an N-terminal His-tag was generated by following Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). The suspension cultures of Sf9 were maintained in Sf-900 III serum-free media (SFM ...
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bioRxiv - Neuroscience 2020Quote: ... The genomic titers of DNase-resistant recombinant AAV particles were determined after alkaline treatment of virus particles and subsequent neutralization by qPCR using the qPCRBIO SY Green Mix Hi-Rox (Nippon Genetics Europe GmbH) and an ABI PRISM 7900HT cycler (Applied Biosystems). Vectors were quantified using primers specific for the GCaMP6s sequence (5’-CACAGAAGCAGAGCTGCAG and 5’-actggggaggggtcacag) ...
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bioRxiv - Neuroscience 2019Quote: ... The cDNA fragment encoding the full-length Npbwr2 was PCR-amplified and subcloned into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific). The resulting Npbwr2 expression construct was transiently transfected into HEK293T cells together with the cAMP-responsive luciferase reporter vector pGL4.29 (Promega ...
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bioRxiv - Pathology 2019Quote: ... The pooled PCR products were added to 9.9 μL of Hi-Di formamide and 0.1 μL of GeneScan™ 500 LIZ™ size standard (Life Technologies). Samples were denatured at 94 C for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Emulsion PCR and Ion Sphere Particles (ISP) enrichment were done using the Ion PGM Hi-Q Kit for 200bp (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mg of His-SUMO-SecA purified from untreated cells was bound to 100 μl Ni-NTA agarose beads (Life Technologies). The beads were incubated with 500 μl of lysis buffer or buffer containing 2 mM sodium azide for 10 minutes and washed three times with 500 μl lysis buffer ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... from a cDNA library and cloned into pcDNA™3.1/His expression vectors (Catalog #V38520, Life Technologies Co., Grand Island, NY). Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170 ...
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bioRxiv - Microbiology 2021Quote: ... The sequencing products were purified using 75% isopropanol and resuspended in 12 μl of Hi-Di™ Formamide (Thermo Fisher Scientific). After loading on the Applied Biosystems® 3130 Genetic Analyzer (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... The cells after incubation were washed with FACS buffer and incubated with anti-his Alexa Fluor 647 antibody (Thermo Fisher Scientific) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2021Quote: 500 ng to 2000 ng of Hi-C library DNA or genomic DNA library were concentrated using a vacuum concentrator (Savant SPD 2010, Thermo Scientific) and then re-suspended in 5 μl dH2O ...
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bioRxiv - Microbiology 2020Quote: ... We then resuspended the cell pellet in 9.5mL PBS/2%HI-FBS for counting using the Countess Cell Counting system (Thermo Fisher Scientific). We aliquoted 0.5 mL for Seq-Well ...
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bioRxiv - Microbiology 2021Quote: ... Serum supernatant was collected as described above and incubated with a pellet of 900 μl Dynabeads™ His-Tag Isolation & Pulldown (Invitrogen) equilibrated in wash buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... experiments were performed in total reaction volume of 10μl with qPCRBIO SyGreen Blue Mix Hi-ROX (PCR BIOSYSTEMS) reagent on 384-wells plates (Axygen) using Viia7 (Thermo Fisher Scientific) instrument in standard conditions.
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
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bioRxiv - Biochemistry 2022Quote: ... His6- tagged SPOC domains were purified by affinity chromatography using HisTrap HP column (Cytiva) or His-Pur Ni-NTA resin (Thermo Scientific) equilibrated in 25 mM Tris-Cl pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Cell Biology 2022Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
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bioRxiv - Microbiology 2020Quote: ... samples were sequenced on an Ion PGM Sequencer with Ion PGM Hi-Q View Sequence Solutions (Ion Torrent Life Technologies, USA).
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the serum was collected then affinity purified using His-CSAG1 linked to the amino link plus coupling resins (Thermo scientific, #20501) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Emulsion PCR and Enrichment was performed on the Ion OT2 system Instrument using the Ion PI Hi-Q OT2 200 kit (Thermofisher Scientific). Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length; Thermofisher Scientific). The Ion Proton reads (FASTQ files ...
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bioRxiv - Microbiology 2020Quote: ... The resulting blots were probed with a sdAb as primary antibody and an HRP-linked 6x-His Tag antibody (Thermofisher, HIS.H8) as the secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... Emulsion PCR and enrichment of template-positive particles were performed using an Ion PGM Hi-Q OT2 Kit (Thermo Fisher Scientific) and the Ion OneTouch 2 system (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length; A26433, Thermofisher Scientific). Sequencing reads were trimmed with Prinseq (160 ...
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bioRxiv - Developmental Biology 2020Quote: ... Emulsion PCR and enrichment was performed on the Ion OT2 system Instrument using the Ion PI Hi-Q OT2 200 kit (A26434, Thermofisher Scientific). Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... The resulting product was digested with BamHI and PmeI and cloned into the BamHI/PmeI sites of pEF6/myc-His (ThermoFisher Scientific). The plasmid obtained was named pEF-POLH ...
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bioRxiv - Biochemistry 2021Quote: ... the N-terminal His-tagged protein was purified in a single step protocol using HisPur Ni-NTA resin (Thermo Fisher Scientific), equilibrated at RT ...
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bioRxiv - Bioengineering 2021Quote: ... 1:1000 mouse anti-his capture antibody in PBS was coated onto Immulon 2 HB 96-well plates (Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Genetics 2020Quote: ... the gypsy backbone used in previous publication (11) was amplified and cloned into the NotI/XbaI sites of pAc5.1/V5-His version B (V411020, Thermo Fisher Scientific). To synthesize the final pAc-gypsy-H2B-mCherry vector ...
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bioRxiv - Microbiology 2020Quote: ... and 293T were cultured in high-glucose DMEM (HyClone) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, ThermoFisher Scientific), 100 U/ml penicillin-G ...
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bioRxiv - Immunology 2021Quote: ... Two initial rounds of magnetic-activated cell sorting were performed on the library using decreasing amounts of Ray Biotech RBD-coated His-Tag Dynabeads (Thermo Fisher). The resulting enriched library pool was subsequently sorted using a Sony SH800 Cell Sorter ...