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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for TNF RII Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were visualized using Ponceau stain and Western blot with monoclonal anti-His (Invitrogen MA1-21315), and polyclonal anti-GST (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated overnight at 4°C with 100 ng of 6x His tag monoclonal antibodies (Invitrogen) in PBS and washed the next day ...
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bioRxiv - Microbiology 2024Quote: ... The entire pENTR effector library was then transferred into the pIB/V5-His vector (Invitrogen; Table S4) using LR Clonase II (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... and human tumor necrosis factor-α (TNF-α) used in the incubation of pancreatic β cells and isolated islets were purchased from Invitrogen (Carlsbad, CA, USA). Ca2+ imaging of mouse islets was performed with Fura-2 AM (Fura-2-acetoxymethyl ester ...
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bioRxiv - Cell Biology 2019Quote: ... stable expression was maintained using 0.9 mg/ml Geneticin (G418) added to the standard HEK293 growth medium (MEM, supplemented with 10% FBS, both from Gibco, Life Technologies, Darmstadt, Germany).
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bioRxiv - Cell Biology 2021Quote: HEK293T (ATCC® CRL-3216™) and S1/S2 DKO HEK293 cells were maintained in cell culture in 1X DMEM (41965-039, Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An inducible D540K hERG HEK293 cell line (T-REx D540K hERG HEK) was generated using the Flp-In T-REx system (Thermo Fisher Scientific, MA, USA). The D540K hERG mutant was made site-directed mutagenesis from the wild-type (WT ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were first prepared for transfection by plating into 12-well tissue culture plates (Nunc 12-565-321; Thermo Fisher Scientific, Waltham, MA) at a density of ∼0.5-2 x105 cells per well and grown to ∼80-90% confluence with standard media ...
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bioRxiv - Immunology 2023Quote: RAVV GPΔMuc fused with Strep II and Avi tags was expressed in HEK293S GNTI−/− cells (ATCC, Manassas, VA) using 293fectin transfection reagent and FreeStyle Media (Thermo Fisher Scientific, Waltham, MA). Supernatants were purified using Streptactin XT Resin purification (IBA Lifesciences ...
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bioRxiv - Cell Biology 2020Quote: All cell lines were cultured in DMEM media supplemented with sodium pyruvate and 10% Hi-FBS (Life Technologies). To express protein constructs in living cells ...
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bioRxiv - Genetics 2020Quote: ... Germany) 1 μl of each PCR product was edited with Hi-Di Formamide (20 μl, ThermoFisher, Massachusetts, USA) and Gene Scan 500 ROX dye Size Standard (0.5 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transient transfection with plasmids encoding Affimer-His constructs using Lipofectamine 2000 (ThermoFisher), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEL and K562 cells were all from American Type Culture Collection (ATCC) and cultured in RPMI with 10% HI FBS except for K562 (grown in IMDM (Gibco, #21980-032) with 10% HI FBS ...
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bioRxiv - Molecular Biology 2019Quote: Wild-type and indicated mutants of MmEsco1 were cloned into pEF6/Myc-His B vector (Thermo Fisher Scientific) containing C-terminal myc and His tags ...
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bioRxiv - Synthetic Biology 2021Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...
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bioRxiv - Biochemistry 2020Quote: Both untagged and His-tagged EncFtn proteins were quantified using the Pierce BCA Assay kit (Thermo Fisher Scientific) following the manufacturer’s instructions for the Test-tube procedure ...
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bioRxiv - Biochemistry 2020Quote: ... Uncleaved His-tagged proteins were removed by incubation of the sample with 1 ml HisPur™ (Thermo Fisher) nickel resin for 1h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... rKLK10 with a 6x C-terminal His tag was affinity purified using HisPur Ni-NTA Resin (Thermo Scientific) per the manufacturer’s instruction (Figure S16 ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) with the following modifications ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting cyclic peptide-mRNA:cDNA library was counter-selected 3 times against His Pull-Down Dynabeads (Thermo Fisher), and affinity selected against 200 nM bead-immobilised Retromer for 30 min ...
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bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-NavSp1 was applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing runs were conducted on Proton sequencer using the PI™ Hi-Q™ Sequencing 200 Kit (Thermofisher) following the recommendations of the manufacturer ...
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bioRxiv - Biochemistry 2019Quote: ... Each protein was first purified by metal affinity chromatography using 2ml of His purTM cobalt column (Thermo Scientific). The tag was removed by cleavage with TEV protease followed by purification on a second cobalt affinity chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... and cDNA was immediately synthetized using Hi-capacity RNA-to-DNA™ kit (Applied Biosystems™, ref. 4388950) both according to manufacturers instructions ...
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bioRxiv - Microbiology 2020Quote: ... and sequenced using the Ion PGM Hi-Q View sequencing kit (Life Technologies, Grand Island, NY, United States) with the Ion PGM™ System technology ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies used for the IP western blots are as follows: anti-His monoclonal (1:3000 dilution; ThermoFisher Scientific) and goat anti-mouse HRP (1:15,000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... and sub cloned in BamHI and SalI site of pCDNA3.1/myc-His(A) mammalian expression vector (Invitrogen, USA) to generate pCDNA3.1-PAX9-myc-His(A ...
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bioRxiv - Biochemistry 2021Quote: ... the array was washed and probed with an anti-His primary antibody (Thermo Fisher, MA121315, 1:1000 dilution), after which anti-mouse HRP-conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... PCR was carried out with gene-specific primers (Table 1) using Phusion Hi-Fidelity PCR Mastermix (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: The PCR products obtained by SGA were cloned into the commercial directional vector pcDNA3.1D/V5-His-TOPO according to the manufacturer’s instructions (Invitrogen). The correct pcDNA3.1-Env plasmid used to produce wild type pdsudoviruses were selected by sequencing ...
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... The cytomegalovirus (CMV) promoter was amplified by PCR from the pcDNA 3.1D/V5-His-TOPO plasmid (Life Technologies) with forward primer 5’-AGTAATCAATTACGGGGTCATTAGTTCAT-3’ and reverse primer 5’ CATAGGAGATGCCTAAGCCGGTGGAGCTCTGCTTATATAGACCTC-3’ ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (ACE2, dilution 1:100; TMPRSS2, 1:500; CD147, 1:500; 6x-HIS-tag (Invitrogen MA1-21315), 1:1000 ...
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bioRxiv - Genetics 2020Quote: ... Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific, USA) to remove the His-tag ...
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bioRxiv - Immunology 2022Quote: ... Wildtype and mutant coding sequences were transferred to the pEF1/V5-His vector (cat. no. V920; Life Technologies) for overexpression ...
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bioRxiv - Synthetic Biology 2022Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... We diluted anti-stem Abs (starting at the lowest concentration, which yielded measurable HI) in C1q-DPBS (Gibco) (220 nM) ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription for other transcripts was performed using the hi-capacity cDNA reverse transcription kit (Applied Biosystems #4368814) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: His-CLASP1 protein was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with a pMT Puro His-PC-SNAP-Elp3 vector using ExpiFectamine™ Sf(A389l5, Gibco) using manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of chicken anti-His antibody (Invitrogen, PA1-9531) on ice for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... entry clones containing individual TSWV proteins were recombined with a pIB/V5-His destination vector (Thermo Fisher Scientific) containing either GFP or mRFP in both amino and carboxy terminal fusions when possible ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Microbiology 2023Quote: ... TCID50) were calculated by IFA using HIS α NDV and Alexa FluorTM 488 α-rabbit secondary antibody (Invitrogen) in two independent experiments.