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Citations for Thermo Fisher :
1551 - 1600 of 5842 citations for Serpin B6 Human Trx His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Human iPSC lines were cultured in StemflexTM medium (ThermoFisher) and passaged approximately every 3 days (1:8–1:12 split ratio) ...
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bioRxiv - Immunology 2023Quote: ... PE-Cy7 anti-human C27 (clone O323, ThermoFisher Scientific), AlexaFluor 700 anti-human CD38 (clone LS198-4-3 ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... previously coated with human type I collagen (Gibco, A1048301). ALI was established once cells reached full confluency by removing media from the apical chamber and replacing media in the basolateral chamber with Pneumacult-ALI maintenance medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... 50 ng/mL human IL-21 (Thermo Fisher Scientific), 50 ng/mL human IL-4 (Miltenyi) ...
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bioRxiv - Neuroscience 2023Quote: ... human Bmp7 (100 ng/ml, ThermoFisher Scientific, 120-03P) or human Semaphorin3d (400 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ng/ml human Tgfb2 (ThermoFisher Scientific, 100-35B), 10 ng/ml murine Csf1 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... human Pleiotrophin (100 ng/ml, ThermoFisher Scientific, 450-15), human Midkine (100 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... human Midkine (100 ng/ml, ThermoFisher Scientific, 450-16), human Bmp7 (100 ng/ml ...
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bioRxiv - Immunology 2023Quote: 6.5 million human embryonic kidney (HEK) 293 cells (Gibco) were seeded the day before transfection in 150mm dishes in DMEM (Gibco ...
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bioRxiv - Genomics 2023Quote: ... The Human Gene 2.1 ST Array platform from Affymetrix produced the data ...
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bioRxiv - Genomics 2023Quote: ... and Human Brain Reference RNA (QS0611, Thermo Fisher Scientific) were used ...
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bioRxiv - Neuroscience 2024Quote: ... Cre-expressing Human embryonic kidney (HEK, Ambion #SC004-BSD) cells were transfected with plasmids expressing cDNA and either shLacZ or the shRNA against the target gene ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and goat anti-human AlexaFluor488 (1:500, Invitrogen A11013) secondary antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μg/ml of human ⍺-CD28 (Thermo Fisher Scientific) and 200 ng of IL-2 (Immunotools ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μg/ml of human ⍺-CD3 (Thermo Fisher Scientific), 5 μg/ml of human ⍺-CD28 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Cell Biology 2024Quote: ... human IL-6 Uncoated ELISA (88-7066-88, Invitrogen) and human GRO alpha (CXCL1 ...
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bioRxiv - Genetics 2024Quote: ... whereas a human CSNK2A2 pre-designed assay (Hs00176505_m1, ThermoFisher) was used as the housekeeping control.
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bioRxiv - Genomics 2024Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher PHG0311), and 10% fetal bovine serum ...
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bioRxiv - Immunology 2024Quote: ... and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher) at a 1:1 bead to cell ratio ...
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bioRxiv - Immunology 2024Quote: ... with anti-human CD107a-eFluor660 (clone H4A3, Invitrogen, UK) at an E:T ratio of 1:1 for a total of 4 hours at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... Human neonatal epidermal keratinocytes (HEKn, Thermo Fisher Scientific, C0015C) were cultured in EpiLife Medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Human neonatal dermal fibroblasts (HDFn, Thermo Fisher Scientific, C0045C) were cultured in Medium 106 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Coding sequence for sbp2 was PCR amplified from 0-5 dpf pooled cDNA using the forward and reverse primers shown in Table 1 and then TA cloned into pcDNA3.1 V5/His Topo (Thermo Fisher Scientific). For secisbp2l ...
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bioRxiv - Molecular Biology 2021Quote: ... biotin labelled Hi-C 3.0 DNAs were purified by 20 μl Dynabeads® MyOne™ Streptavidin C1 beads (Thermo Fisher 65002). The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1.5ml of SD/-Leu/-His liquid medium (plus 3 mM 3-AT) were incubated in a 12-well microplate (Thermo Scientific) at 30°C for 96 hours (h) ...
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bioRxiv - Molecular Biology 2020Quote: Approximately 4 x 105 HEK293T cells in a 6-well plate were transfected with 250 ng/well of pMT107-octa-6 His-ubiquitin or 200 ng/well of pcDNA3-2x-6His-ubiquitin constructs using 50 µl/well Opti-MEM (Gibco; 31985062) and 2 µl/well of FuGENE 6 Transfection reagent (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant baculovirus containing the sequence of ΔPRD-dynamin 1 with an N-terminal His-tag was generated by following Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). The suspension cultures of Sf9 were maintained in Sf-900 III serum-free media (SFM ...
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bioRxiv - Neuroscience 2020Quote: ... The genomic titers of DNase-resistant recombinant AAV particles were determined after alkaline treatment of virus particles and subsequent neutralization by qPCR using the qPCRBIO SY Green Mix Hi-Rox (Nippon Genetics Europe GmbH) and an ABI PRISM 7900HT cycler (Applied Biosystems). Vectors were quantified using primers specific for the GCaMP6s sequence (5’-CACAGAAGCAGAGCTGCAG and 5’-actggggaggggtcacag) ...
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bioRxiv - Neuroscience 2019Quote: ... The cDNA fragment encoding the full-length Npbwr2 was PCR-amplified and subcloned into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific). The resulting Npbwr2 expression construct was transiently transfected into HEK293T cells together with the cAMP-responsive luciferase reporter vector pGL4.29 (Promega ...
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bioRxiv - Pathology 2019Quote: ... The pooled PCR products were added to 9.9 μL of Hi-Di formamide and 0.1 μL of GeneScan™ 500 LIZ™ size standard (Life Technologies). Samples were denatured at 94 C for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Emulsion PCR and Ion Sphere Particles (ISP) enrichment were done using the Ion PGM Hi-Q Kit for 200bp (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mg of His-SUMO-SecA purified from untreated cells was bound to 100 μl Ni-NTA agarose beads (Life Technologies). The beads were incubated with 500 μl of lysis buffer or buffer containing 2 mM sodium azide for 10 minutes and washed three times with 500 μl lysis buffer ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... from a cDNA library and cloned into pcDNA™3.1/His expression vectors (Catalog #V38520, Life Technologies Co., Grand Island, NY). Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170 ...
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bioRxiv - Microbiology 2021Quote: ... The sequencing products were purified using 75% isopropanol and resuspended in 12 μl of Hi-Di™ Formamide (Thermo Fisher Scientific). After loading on the Applied Biosystems® 3130 Genetic Analyzer (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... The cells after incubation were washed with FACS buffer and incubated with anti-his Alexa Fluor 647 antibody (Thermo Fisher Scientific) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2021Quote: 500 ng to 2000 ng of Hi-C library DNA or genomic DNA library were concentrated using a vacuum concentrator (Savant SPD 2010, Thermo Scientific) and then re-suspended in 5 μl dH2O ...
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bioRxiv - Microbiology 2020Quote: ... We then resuspended the cell pellet in 9.5mL PBS/2%HI-FBS for counting using the Countess Cell Counting system (Thermo Fisher Scientific). We aliquoted 0.5 mL for Seq-Well ...
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bioRxiv - Microbiology 2021Quote: ... Serum supernatant was collected as described above and incubated with a pellet of 900 μl Dynabeads™ His-Tag Isolation & Pulldown (Invitrogen) equilibrated in wash buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... experiments were performed in total reaction volume of 10μl with qPCRBIO SyGreen Blue Mix Hi-ROX (PCR BIOSYSTEMS) reagent on 384-wells plates (Axygen) using Viia7 (Thermo Fisher Scientific) instrument in standard conditions.
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
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bioRxiv - Biochemistry 2022Quote: ... His6- tagged SPOC domains were purified by affinity chromatography using HisTrap HP column (Cytiva) or His-Pur Ni-NTA resin (Thermo Scientific) equilibrated in 25 mM Tris-Cl pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Cell Biology 2022Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...