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Citations for Thermo Fisher :
1551 - 1600 of 7001 citations for ST2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml human bFGF (Invitrogen), and 50 ng/ml recombinant human FGF-10 protein ...
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bioRxiv - Microbiology 2023Quote: ... Human Plasma-like Media (HPLM, GIBCO) was used as base media and supplemented with 2 mM glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD326 (EpCAM) (#12932642, Invitrogen), and anti-human CD340 (HER2 ...
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bioRxiv - Immunology 2023Quote: Primary human fibroblasts (Thermo fisher; C0135C) were cultured in high glucose DMEM (Dulbecco’s modified media ...
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bioRxiv - Biophysics 2023Quote: ... 10 mg/ml human insulin (Gibco), 20 ng/ml EGF (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... Dynabeads human T-Activator (Thermo Fisher) anti-CD3/CD28 beads (at a 1:1 bead/cell ratio) ...
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bioRxiv - Biochemistry 2023Quote: ... Expi293F™ human cells (ThermoFisher Scientific) were grown in Expi293™ medium (Gibco ...
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bioRxiv - Genomics 2023Quote: Human Total Brain RNA (ThermoFisher Scientific) and a 48,502 bp double-stranded linear DNA from Bacteriophage Lambda (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... human CD45 (clone HI30; Thermo Fisher); mouse CD45 (clone I3/2.3 ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µg/mL human insulin (Gibco), and 1x pen/strep ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ42 human ultrasensitive ELISA Kit (Thermofisher), and D2R (Mybiosource) ...
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bioRxiv - Cell Biology 2023Quote: ... and human plasma fibronectin (Life Technologies) coated plastic culture plates or Ø 0.4 μm or Ø 3.0 μm pore Transwell inserts (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human astrocytes (Thermo Fisher, N7805100) were used for the co-culture conditions and grown in DMEM supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... The GLOBINclear Human Kit (ThermoFisher Scientific) was then employed to deplete human globin mRNA in all samples ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human BMP4 (Thermo Fisher Scientific) at 10 ng/ml was added every third day in the presence of EGF and bFGF in the media ...
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bioRxiv - Cancer Biology 2024Quote: ... human (Thermo Fisher Scientific, cat# 4351379) using an Applied Biosystems™ QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... human embryonic kidney 293A cells (Invitrogen) were transfected with pHelper ...
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bioRxiv - Cancer Biology 2024Quote: ... and Human FoxP3 EF450 (Invitrogen 48477642). Cells were stained with viability dye Zombie NIRtrade Fixable Viability Kit (Biolegend Inc ...
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bioRxiv - Microbiology 2024Quote: ... Heat stable recombinant human bFGF (Gibco) at 5U/ml was supplemented to organoid formation media only on day 0 ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells (CRL-11268™, ATCC®. Gaithersburg, Maryland, US) were cultured in DMEM high glucose (31966-021, Gibco™, Thermo Fisher, UK), supplemented with 10% FBS (16140-071 ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells (CRL-11268™, ATCC®. Gaithersburg, Maryland, US) were cultured in DMEM high glucose (31966-021, Gibco™, Thermo Fisher, UK), supplemented with 10% FBS (16140-071 ...
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bioRxiv - Cell Biology 2019Quote: ... stable expression was maintained using 0.9 mg/ml Geneticin (G418) added to the standard HEK293 growth medium (MEM, supplemented with 10% FBS, both from Gibco, Life Technologies, Darmstadt, Germany).
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bioRxiv - Genomics 2022Quote: Frozen HEK293 or K562 cell pellets were lysed in 1 mL lysis buffer supplemented with 5 µl protease inhibitor cocktail (Thermo Fisher cat# 87786) and 10 µl Rnase inhibitor (NEB cat# M0314B ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... SK-Hep1, Huh7, MHCC-97H, and HEK293 cells were cultured in DMEM (HyClone, Logan, UT, USA) supplemented with 10% FBS (Gibco, Rockville, MD, USA), 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells stably expressing CMV:DOR-APEX2 were created as previously described and maintained in 1:1000 G418/Geneticin (Thermo Fisher Scientific, Cat # 10131035) (Lobingier et al ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: The plasmids coding for pro-BMP9 and pro-BMP10 were co-transected in equal amounts into HEK293 cells (expi293, Invitrogen/Thermo Fisher Scientific) and after five days ...
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bioRxiv - Immunology 2023Quote: ... Gregoire Lauvau’s lab) and HEK293 Phoenix Ampho Packaging Cells (provided by Prof. Andrew Ward) were cultured in complete DMEM medium (Gibco, Cat#10569-010) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC; CRL-1573™) were cultured in complete DMEM (cDMEM) in Nunc EasYFlask T75 cell-culture flasks (Thermo Scientific; cat # 156499) and housed in CO2 incubator (5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... DNA samples containing the extension products were added to Hi-Di formamide (Applied Biosystems, Foster City, CA). The mixture was incubated for 5 min at 95°C ...
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bioRxiv - Cell Biology 2020Quote: ... Air-liquid interface medium consisted of 50% BEGM and 50% hi-glucose minimal essential medium (DMEM, Gibco) containing 100 nM additional retinoic acid (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... parahaemolyticus POR1 genomic DNA and inserted into the multiple cloning site (MCS) of pBAD/Myc-His (Invitrogen) vector containing a kanamycin-resistance cassette 25 (in-frame or out-of-frame with the C-terminal Myc-His tag ...
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bioRxiv - Genetics 2020Quote: ... The human PROS1 cDNA (GenBank NM_000313.4) was PCR-amplified from HepG2 cDNA and cloned into pcDNA3.1/myc-His(−) plasmid (Invitrogen). The PCR amplification of PROS1 from c.-44 to c.2028 was performed using Phusion High Fidelity (HF ...
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bioRxiv - Developmental Biology 2021Quote: ... The others were TA-cloned to pcDNA3.1/V5-His TOPO TA vectors (Cat. No. K4800-01, Invitrogen). HEK293T cells were cultured in DMEM medium with 10% FBS in 5% CO2 at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The araC gene and araBAD promoter were PCR-amplified from pBAD/His/lacZ (Invitrogen, Carlsbad, CA, USA). The lac promoter and inhibitory unit (iUnit ...
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bioRxiv - Developmental Biology 2020Quote: ... The template was prepared using the Ion PI Hi-Q OT2 200 kit (Thermo Fisher Scientific, UK). Sequencing was performed using the Ion PI Chip Kit v3 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
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bioRxiv - Bioengineering 2019Quote: ... and cloned into the standard mammalian expression vector pEF1/myc-His B (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Biochemistry 2019Quote: His-tagged SIVsmm Nef constructs were expressed in BL21 (DE3) star cells (Life technologies, Grand Island, NY), and induced with 0.3 mM IPTG at 25 °C overnight ...
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bioRxiv - Immunology 2021Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermo Fisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS.51 S1-S2-His (referred as S ...
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bioRxiv - Cell Biology 2020Quote: ... The protease was removed using His-Pur™ Ni-NTA agarose resin (Thermo Fisher Scientific, Waltham, MA). Gle1152-360 was further purified by a 30% ammonium sulfate precipitation ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293T cells were cultured in DMEM (HyClone) supplemented with 10% heat-inactivated FBS (HI-FBS; ThermoFisher). CHLA-255-Luc-EGFP cells were cultured in IMDM (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The stable cell lines were generated using the pcDNA/FRT/V5-His TOPO TA Expression kit (Invitrogen) performed according to the manufacturer’s protocol and as described previously (28) ...
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bioRxiv - Plant Biology 2021Quote: ... Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels, Invitrogen) and gels were stained with Quick Coomassie Stain (Generon) ...
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bioRxiv - Microbiology 2021Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in Express-Five media (Life Technologies) and infected with 0,5% vol/vol of generation 2 (V1 ...
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bioRxiv - Neuroscience 2020Quote: ... CCM2 isoforms were inserted into pcDNA3.1/V5-His-TOPO and Topo pCR®II plasmid vectors (Invitrogen) respectively (1-6 ...
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bioRxiv - Molecular Biology 2020Quote: The sequence of the OpIE2 promoter was obtained from pIB/v5-His map sequence (ThermoFisher Scientific, USA). The truncated version of the wild-type OpIE2 promoter sequence (tIE2) ...
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bioRxiv - Microbiology 2020Quote: ... The templates were sequenced with an Ion PGM Hi-Q View Sequencing Kit (Thermo Fisher Scientific Inc.) and a 318 Chip Kit v2 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... Ribosome nascent chain complexes (RNCs) were purified via the encoded His-Tag using magnetic Dynabeads (Invitrogen, #10104D). The translation reaction was applied to equilibrated beads (50 mM HEPES pH 7.5 ...