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Citations for Thermo Fisher :
1551 - 1600 of 6974 citations for Recombinant Human OXSR1 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and STRN3 were carried out utilising immobilised recombinant protein A/G resin (Thermo Scientific). Cell pellets were harvested and processed as described in the EGFP co-immunoprecipitation section ...
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bioRxiv - Immunology 2023Quote: ... Recombinant antigen was immobilized on N-hydroxysuccinimide (NHS)–activated agarose resins (Thermo Fisher, 26197) by overnight rotation at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained with anti-CD64 (CD64 Recombinant Rabbit Monoclonal Antibody, Thermofisher, # MA5-29706) at a dilution of 1:400 in 1% BSA in PBS and incubated at 4°C overnight in the dark overnight ...
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bioRxiv - Immunology 2023Quote: ... recombinant bacmid DNA was generated using the Bac-to-Bac system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Recombinant RBD (Arg319 – Phe541) of the same strain was obtained from Invitrogen (# RP 87678) and used as an immunogen without conjugation with KLH and used for screening assays ...
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bioRxiv - Neuroscience 2024Quote: ... An injection of Cholera Toxin Subunit B (recombinant) conjugated to Alexa Fluor 647 (ThermoFisher) was made in the striatum of the same hemisphere (+1.2mm AP ...
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bioRxiv - Microbiology 2024Quote: Recombinant ACE2 protein was coated on 96-well NUNC Maxisorp plates (Thermo Fisher Scientific) at 100 ng per well overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was cleared with recombinant protein G– conjugated agarose beads (Invitrogen, cat# 15920-010) and subsequently immunoprecipitated with H3K27ac antibody (Active Motif ...
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bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots were examined on a 10% SDS-PAGE followed by Coomassie Blue staining or Western Blot analysis with rabbit α-GST-HRP antibody (Life Technologies, Carlsbad, CA) at a 1:50,000 dilution in Tris-buffered saline (20 mM Tris ...
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bioRxiv - Pathology 2022Quote: Cells were transfected with the GST fusion protein construct or the BRET-based cAMP construct (1 μg) using Lipofectamine 2000 (Invitrogen Life Technologies, Saint Aubin, France) in Opti-MEM medium.
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bioRxiv - Biophysics 2021Quote: ... mCherry-tagged protein-expressing Neuro2a cells were incubated in a medium containing 0.5 μM Lysotrascker green DND-26 (Thermo Fisher) for 30 min before image acquisition.
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bioRxiv - Developmental Biology 2021Quote: Immunoprecipitations from HEK293T cells expressing either HA-or FLAG-tagged Yap were performed using the Dynabeads Protein A Immunoprecipitation Kit (Invitrogen) by coupling 5 μg of anti-FLAG (Cell signaling ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: mouse anti-V5-tag to amplify V5-tagged CFP (Thermo Fisher, R96025, 1:650), rat anti-mCherry (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Neuroscience 2019Quote: ... Chicken anti-rabbit tagged with Alexa Fluor 488 and goat anti-mouse tagged with Alexa Fluor 568 were used as secondary antibody at a dilution of 1:1000 (Invitrogen). Tissues were incubated with these secondary antibodies for 48 hours at 4 °C followed by regular washing ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged full-length TRIM21 was cloned into a pFastBac vector for expression in the baculovirus system (Bac-to-Bac, Invitrogen), resulting in pFastBac-MBP-TRIM21 ...
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bioRxiv - Molecular Biology 2020Quote: ... Large-scale expression of Strep-tagged proteins for purification was conducted in Trichoplusia Ni High Five cells (Thermo Fischer Scientific) in SF-900 medium (Gibco). Cells at a density of 2×106 cells/ml were infected with 1.5% of amplified virus and incubated for 40 hours ...
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bioRxiv - Bioengineering 2022Quote: ... supernatants were harvested and filtered with a 0.22 µm membrane.The His-tagged proteins were purified with the HisPur Ni- NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IP6K1 was sub-cloned into SFB (S-protein/FLAG/SBP) - tagged destination vector using the Gateway cloning strategy (Invitrogen). Catalytically inactive IP6K1 (K226A ...
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bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... with Strep and His epitope tags was amplified by PCR from pIEx10-LRIM1 (Povelones et al. 2009) with BamHI tagged primers (LRIM1-F, LRIM1-R) and subcloned into pJET1.2 (Thermo Scientific) before cloning into pslVg-BGH-attP ...
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bioRxiv - Molecular Biology 2019Quote: BGH was amplified by PCR from pslVg-GFP-BGH by PCR with BamHI and NotI tagged primers (BGH-F, BGH-R) and subcloned into pJET1.2 (Thermo Scientific). attP was amplified from pBCPB+ (Groth et al ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid encoding for FLAG-tagged TM or IM-TM were transfected into EXPI293F cells according to the manufacturer (Thermo Fisher), and incubated at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody solution (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2 μg His-tagged calcineurin was first bound to Ni-NTA-agarose or magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... mCherry-(backbone pLV-CMV-bc) and FLAG-His-tagged(backbone Pcdna3) p16INK4A constructs were created using gateway technology (Life Technologies). For cell cycle profiling ...
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bioRxiv - Molecular Biology 2019Quote: T7-tagged cDNA template was PCR amplified and subjected to in vitro transcription using a MEGAscript T7 kit (Applied Biosystems). Amplified cRNA was purified with an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were next incubated in biotinylated secondary antibody for 1 hour at room temperature followed by incubation in fluorophore tagged horseradish peroxidase (Alexa Fluor; Thermofisher). Sections were washed thrice in PBS-T and counterstained with RNAscope DAPI for 30 seconds ...
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bioRxiv - Biochemistry 2020Quote: ... pcDNA5/FRT/TO plasmid encoding Flag-tagged WT ExoU or S142A ExoU was incubated in serum free medium containing lipofectamine 2000 (Invitrogen) for 30 minutes at ambient temperature ...
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bioRxiv - Biochemistry 2020Quote: MBP-tagged (pTHMT) full-length SARS-CoV2 nucleocapsid protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Bioengineering 2021Quote: ... supernatants were harvested and filtered with a 0.22 μm membrane.The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were co-transfected with either a constant concentration of 0.1 μg of NF1-NanoLuc pcDNA expression plasmid or CRAF RBD-NanoLuc pcDNA expression plasmid with 0.2 μg of GFP-tagged KRAS (WT or Mutant) with 0.25 μl of Lipofectamine 2000 per well following the manufacturer’s protocol (Thermo Fisher Scientific). Twenty-four hours later ...