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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Pig Hemoglobin HB CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... using the Ion PGM Sequencing 400 Kit and deposited in two Ion 318 chip Kit v2 following the manufacturer’s protocol (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: RNA extraction was done using Qiagen RNeasy kit and equal amounts of RNA were reverse transcribed using the Maxima Reverse Transcriptase kit (ThermoFisher). Semi-quantitative qPCR was performed using PerfeCTa SYBR Green SuperMix (QuantaBio) ...
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bioRxiv - Cancer Biology 2022Quote: ... reagent/kit and reverse transcribed to complementary DNA (cDNA) using a High Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, #4368813). The expression of PYGL (assay ID ...
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bioRxiv - Microbiology 2022Quote: ... Luminex kits that were used are as follows a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was then diluted 1:5 in 1x Assay buffer and processed according to the kit instructions (DNA Damage Competitive ELISA Kit, Invitrogen). The initial DNA concentration used in the assay was optimized to ensure the resulting values fall within the optimum range of kit detection limits (between Std3 and Std6) ...
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bioRxiv - Cell Biology 2022Quote: ... and total RNA was extracted using a commercial kit (PureLink™ RNA Mini Kit, Ambion, Life Technologies, Carlsbad, CA, USA). After RNA extraction ...
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bioRxiv - Cell Biology 2022Quote: ... and total RNA was extracted using a commercial kit (PureLink™ RNA Mini Kit, Ambion, Life Technologies, Carlsbad, CA, USA). After RNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted using the RNeasy Mini Kit and treated with the DNase from the Turbo DNA-free Kit (Invitrogen) according to the manufacturer’s ‘rigorous DNase treatment’ protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and quantity was assessed using Agilent BioAnalyzer RNA 6000 Pico Kit and the Invitrogen Qubit RNA HS Assay kit (Invitrogen), respectively ...
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bioRxiv - Genetics 2022Quote: ... The pellet was then either processed with the Subcellular Protein Fractionation Kit for Cultured Cells kit (Thermo Fisher Scientific, 78840) or resuspended in 1x RIPA buffer with protease inhibitors to extract whole cell lysate.
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bioRxiv - Microbiology 2019Quote: ... The pooled samples were cleaned with the MagBio Beads Cleanup Kit (MagBio) for a second time and quantified again using the dsDNA HS Assay kit for Qubit fluorometer (Invitrogen). The resulting library was sent to Eurofins genomics (Konstanz ...
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bioRxiv - Cell Biology 2019Quote: ... The cell pellet was then either processed with the Subcellular Protein Fractionation Kit for Cultured Cells kit (Thermo Scientific, 78840), or resuspended in 1 x RIPA buffer with protease inhibitors to extract whole cell lysate ...
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bioRxiv - Cell Biology 2019Quote: ... All cells were authenticated by STR profiling (GenePrint 10 System kit from Promega and AuthentiFiler PCR Amplification Kit from ThermoFisher) and tested negative for mycoplasma contamination using the Cell Culture Contamination Detection Kit (ThermoFisher).
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bioRxiv - Immunology 2020Quote: ... IL-1β ELISA kit (Catalog # 88-7013-22) and TNFα ELISA kit (Catalog # 88-7324-88) were from Invitrogen (America). LDH Cytotoxicity Assay Kit (C00170 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader.
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend], Zombie NIR fixable viability kit [Biolegend], eBioscience fixable viability dye eFluor 780 [Invitrogen]). Samples were analysed using a BD X-20 flow cytometer and data analysis was performed using FlowJo v10 (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend], Zombie NIR fixable viability kit [Biolegend], eBioscience fixable viability dye eFluor 780 [Invitrogen]). Samples were analysed using a BD X-20 flow cytometer and data analysis was performed using FlowJo v10 (BD Biosciences).
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bioRxiv - Microbiology 2019Quote: ... The viability of bacteria in the particulate matters was studied using a live/dead viability kit (L7012 BacLight Viability Kit, Invitrogen) as described in Supplementary Text ...
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bioRxiv - Genetics 2020Quote: Total RNA was isolated from 5 × 106 log-stage parasites with the NEB Monarch Total RNA miniprep kit and contaminating genomic DNA was eliminated with the TURBO DNA-free kit from ThermoFisher. Samples were then subjected to first-strand cDNA synthesis using the Applied Biosystems High Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Genetics 2020Quote: ... Detection was done by either Western Breeze chromogenic kit or chemiluminescence kit (Pierce™ ECL Western Blotting Substrate, Thermo Fisher) using Kodak BioMax Light film.
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bioRxiv - Genetics 2020Quote: ... and mRNA was synthesized using an in vitro RNA transcription kit (mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit, AM1345, Invitrogen). sgRNAs were amplified and transcribed in vitro using the MEGAshortscript™ T7 Transcription Kit (AM1354 ...
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bioRxiv - Cell Biology 2020Quote: ... NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit) and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion). For GFP-centrin ...
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bioRxiv - Neuroscience 2021Quote: Cell viability was determined using a commercially available dual-wavelength fluorescent kit (LIVE/DEAD Viability/Cytotoxicity Assay Kit; L3224; Invitrogen). Live cells are detected by intracellular esterase activity using the dye calcein-AM (∼495/515 nm ex/em ...
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bioRxiv - Systems Biology 2021Quote: ... DNA was extracted from 1 mL of this outgrown culture using a GeneJet Genomic DNA Purification Kit (ThermoScientific) and the concentration of genomic DNA was quantified using a Qubit dsDNA HS Assay Kit (Invitrogen). A 1 μg aliquot of genomic DNA was suspended in 15 μL of 10 mM Tris buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were transcribed with the MEGAshortscript T7 Kit from synthetic DNA oligomers and purified with the MEGAclear Transcription Clean-Up Kit (both ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... nuclear soluble and chromatin-bound protein fractions were extracted from cells using the Subcellular Protein Fractionation Kit for Cultured Cells kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJet Plasmid Miniprep kit (Thermo K0503) or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). Site-directed mutagenesis of plasmids was performed by DNA synthesis with 2.5 U PfuUltra II Fusion HS DNA Polymerase (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... Each plasmid was linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription with 1 µL of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Genomics 2021Quote: ... total RNA was extracted from DIII pearlfish embryos with a Qiagen RNeasy mini plus kit and reverse transcribed with a superscript IV VILO kit from ThermoFisher according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers ORF1ab_Fw ...
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bioRxiv - Immunology 2022Quote: ... Kits and murine and human serum CD40L was quantified using the commercially available soluble CD40L ELISA Kits (Thermo Fisher Scientific), following the manufacturer’s procedure.
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from pooled 7-day old seedlings using the Qiagen RNeasy Plant Mini Kit and complementary DNA was synthesized using the Maxima cDNA Synthesis kit (Thermofisher). Quantitative RT-PCR analysis was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein in brain tissue was determined using a protein assay kit (Pierce™ BCA Protein Assay Kit; ThermoFisher, 23225) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... and nuclear compartments using consecutive centrifugation steps of varying speeds and specialized buffers obtained from a commercially available kit (Sub-Cellular Protein Fractionation Kit for Tissues, ThermoFisher). Bradford protein assays were performed for each compartment individually ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was removed by the DNase treatment kit (DNA-free™ DNA Removal Kit, Invitrogen, Thermo Fisher Scientific, Massachusetts, USA). Parasites were synchronized by 5% sorbitol (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... ABE8e or ABE9 mRNA was transcribed using the in vitro RNA transcription kit (mMESSAGE mMACHINE® T7 Ultra Kit, Ambion). mRNA was purified with MEGAclear™ Kit (Ambion) ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was prepared with a Qiagen RNeasy kit and cDNA synthesis was performed with a SuperScript VILO kit (Life Technologies), per the manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: ... 0.1-1.0μg of RNA was converted to cDNA using the iScript cDNA synthesis kit (Bio Rad) or the High-Capacity RNA-to-cDNA kit (ThermoFisher). qPCR was done using either SsoFast EvaGreen supermix and a BioRad CFX96 Real Time System (Vancouver) ...
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bioRxiv - Immunology 2023Quote: ... Total protein quantification was determined using Pierce™ BCA Protein Assay Kit (Thermo Scientific Pierce™ BCA Protein Assay Kit) and samples were stored at -80°C until use.
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bioRxiv - Neuroscience 2023Quote: ... and the cRNA was generated from each plasmid by using an RNA preparation kit (mMessage mMachine T7 Transcription Kit; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the appropriate band was purified (ZymoClean Gel DNA Recovery Kit, ZymoResearch) before quantification by fluorescence (Qubit dsDNA HS Kit, Invitrogen).9
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bioRxiv - Molecular Biology 2023Quote: The TaqMan assays were performed using a one-step TaqMan probe kit (TaqManTM RNA-to CTTM 1-Step Kit, Invitrogen). As the TaqMan probe we used 12➔7 Probe FAM–ACCATCAGCCCCCTTTTTATTTCCT-MGBNFQ ...
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bioRxiv - Neuroscience 2023Quote: ... Lumbar spinal cords were dissected in oxygenated artificial cerebrospinal fluid and immediately homogenized using Cytoplasmic Extraction Reagent Kit from the NE-PERTM nuclear extraction kit (ThermoFisher) with a protease inhibitor cocktail added (5mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... after which the entire pool was purified using the Qiagen MinElute purification kit and quantified via Qubit dsDNA HS assay kit (Invitrogen) and sequenced at Novogene on 1 HiSeq PE 150 lane (6 bp ...
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bioRxiv - Microbiology 2023Quote: The protein content of pEVs were quantified using Pierce BCA kit according to the manufacturer’s instructions (BCA Protein Assay Kit, Thermo Fisher).
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bioRxiv - Developmental Biology 2024Quote: ... PCR products of bisulfite-treated DNA were purified with a Gel Extraction kit (NZY Tech) and cloned into TOPO cloning kit (Thermofisher). Individual clones were sequenced by standard Sanger sequencing and data were analysed by the online tool QUMA 54.
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bioRxiv - Microbiology 2024Quote: ... the PCR products were purified using the E-Z 96 Cycle Pure Kit (Omega) and quantified with Qubit dsDNA HS Assay Kits (Invitrogen). The purified PCR products were then pooled equally for subsequent sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were digested using PreOmics sample kit (iST Kit, preomics) and analyzed in Q ExativePlus mass spectrometer (Thermo Fisher Scientific).