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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... washed and stained with LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (20 mins, 4°C; Thermofisher, #L34965). After washing ...
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bioRxiv - Genomics 2022Quote: ... a total of 20 electroporation reactions was performed using the Neon™ Transfection System 100 µL Kit (Invitrogen, #MPK10025) and the associated Neon™ Transfection System (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Small RNA preparations (20-200 nt length) were carried out using the mirVana miRNA isolation kit (Ambion/Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Small RNA preparations (20-200 nt length) were carried out using the mirVana miRNA isolation kit (Ambion/Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 20-688) was used to prepare sequencing libraries using the Ion Plus Fragment Library Kit (Thermo Fisher Scientific, 4471252), following manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... complementary DNA (cDNA) was generated in a total volume of 20 μl using the commercial kit (Thermo Scientific, EU). The reaction of RT-qPCR was conducted in a 48-well plate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... blocked for 20’ with hydrogen peroxidase (3%, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium), washed twice in 1XPBS for 5’ each and incubated with Goat anti-Rat-Biotin (1:300 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein extracts were centrifuged at 14,000 x g at 4°C for 20 minutes and quantified with Pierce BCA Protein Assay Kit (ThermoFisher). 10 μg or 20 μg of proteins were separated by electrophoresis in denaturing SDS polyacrylamide gel (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Purified and concentrated EGFP_Nanobody protein was immobilized onto agarose beads (20 mg protein per column) using AminoLink Plus Immobilization Kit (ThermoFisher), following manufacturer’s instructions in PBS pH 7.5 coupling buffer.
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bioRxiv - Physiology 2024Quote: The conversion of RNA to cDNA was carried out with Superscript IV Vilo kit using 1µg of total RNA in a reaction volume of 20 µl as per manufacturer’s instructions (Invitrogen #11766050). The reaction mixture in 4 µl consisting of Mgcl2 ...
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bioRxiv - Genetics 2019Quote: ... pJF and pSM were generated by cloning PCR products into the pCR BluntII vector backbone using the Zero Blunt™ Topo™ system (Thermo Fisher Scientific). pSDH donor cassette plasmids were cloned by ligating PCR products into pSDH76 ...
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bioRxiv - Systems Biology 2021Quote: ... 5 mg and 20 mg pieces then washed twice in 20 tissue volumes (20 µL/mg) Mg2+/Ca2+-free DPBS containing 1X HALT protease and phosphatase inhibitor (Thermo Fisher Scientific, Waltham, MA, USA). After washing ...
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bioRxiv - Evolutionary Biology 2019Quote: ... cDNAs were synthesized from 5 μg of total RNA or 20 ng of mRNA in 20 uL reaction volume using the SuperScript III First-strand synthesis System (#18080051; Thermo Fisher scientific, Waltham, MA, USA) with the oligo (dT20 ...
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bioRxiv - Physiology 2023Quote: ... Excess dye was removed via cold acetone precipitation (−20°C for 20 min) and samples were resuspended in 50μL of 1X reducing buffer (Invitrogen loading dye and 5% β-mercaptoethanol), heated for 5 mins (95°C ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: Flash-frozen brain tissue was homogenized with a mechanical handheld homogenizer for 20 seconds in RNA kit lysis buffer (PureLink™ RNA Mini Kit, Life Technologies, Carlsbad, CA) plus 1% β-mercaptoethanol ...
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bioRxiv - Bioengineering 2019Quote: ... Hydrogels were subsequently washed in a 0.1% Tween 20 solution in PBS for 20 minutes 4 times and incubated in a 1:500 dilution of goat anti-mouse secondary antibody (Thermo Fisher A-11001) protected from light overnight at 4°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... Tween 20 for 30 min at room temperature and then incubated with mouse anti-6xHis tag monoclonal antibody-HRP conjugate (Thermo Fisher Scientific, USA) in TBS-T with 0.05 % BSA for 2 h at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... dry milk and 0.1 % (v/v) Tween 20 and then incubated with mouse anti-6xHis tag monoclonal antibody-HRP conjugate (Clontech or Thermo Fisher Scientific, USA) in the same buffer for 2 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the proteasomes were eluted from the magnetic resin by incubating with TEV protease (Invitrogen) in buffer B containing 1 mM ATP for 1 h 30 min at 30 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... according to manufacturer’s instructions then reverse transcribed with an oligo(dT)20 primer using the Superscript III cDNA First Strand Synthesis kit (Invitrogen). The qPCR reaction was set up using the KAPA SYBR FAST ABI Prism kit (KAPA Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... Surveyor assay was performed using Sarm1AG3S PCR conditions and Surveyor Mutation Detection Kit (IDT) followed by electrophoresis on Novex 20% TBE gels (Invitrogen). Off-target CRISPR cleavage was accessed by PCR amplification using the primers listed in table I and cycling conditions 95° 30 sec ...
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bioRxiv - Developmental Biology 2020Quote: The full-length coding region of chicken EYA4 was PCR amplified from HH19-20 somite cDNA using SuperScript III Reverse Transcriptase kit (Invitrogen). Primer design used predicted EYA4 sequences for chicken (ENSGALG00000031656 ...
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bioRxiv - Microbiology 2019Quote: ... were re-suspended in 20 µL of RNAse-free water and treated with DNAse with the DNA-free kit (Ambion) before being stored at -80°C ...
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bioRxiv - Immunology 2019Quote: Annealed oligonucleotides containing the sequence of the HCV 5’ppp poly-U/UC region (Table 1) (20) were in vitro transcribed using the Megashortscript T7 transcription kit (Ambion) followed by ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: Total RNA (20 ng) was used as template to synthesize cDNA using Maxima First Strand cDNA Synthesis Kit (Thermo Scientific). Gene expression of three cyc2 genes (Slit_0263 ...
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bioRxiv - Plant Biology 2021Quote: ... The fragments were introduced into pENTR-D-TOPO vector by pENTR™ Directional TOPO Cloning Kit (K2400-20, Life Technologies). pENTR-D-TOPO-NKS1 with or without stop codon was subsequently cloned into pUBN-GFP/RFP-NKS1 (N-terminal fusion ...
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bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then loaded with the fluorescent substrate by adding 20 µl of 6X CCF4/AM solution (LiveBLAzer-FRET B/G Loading Kit, Invitrogen) containing 15 mM Probenecid (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... approximately 800 ng of DNA products were subjected to 20 µL in vitro transcription reactions according to the manufacturer’s instructions (MEGAscript T7 kit, Ambion/Thermo Scientific). After incubating the samples at 37 °C for 3 h and 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized from 2 μg total RNA in 20 μL reaction mixture using a cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (40ng) was used in 20 µL of reverse transcription reaction (SuperScript IV VILO Master Mix with ezDNase Enzyme kit, Invitrogen) according to the manufacturer’s instruction and 2 µL of cDNA in 20 µL reverse transcription reaction with specific FAM-labelled Taqman probes (Apoe Mm01307193_g1 ...
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bioRxiv - Neuroscience 2020Quote: ... First strand synthesis was performed using Invitrogen a SuperScript III First-Strand Synthesis Kit with Oligo(dT)20 primers (Invitrogen). Quantitative PCR was performed using Sybr Green Supermix (BIO-RAD ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcription of starfish cyclin B (sfcyclin B) mRNA was performed as previously described (20) using the mMESSAGE mMACHINE T7 kit (Invitrogen) with the original NTP concentrations ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA (20 ng/µL) was converted to cDNA using the High Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific) and amplified using specific TaqMan assays (catalog # 4331182 ...
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bioRxiv - Microbiology 2020Quote: ... one or two guanines were added to the 5’ end of the guide sequence within the primer to ensure the format “5’-GG(N18-20)-3’” in order to facilitate in vitro transcription with MEGAscript T7 in vitro transcription kit (Ambion). Transcribed sgRNAs were purified with MEGAclear kit (Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... Purification of cytoplasmic and mitochondrial fractions or pure mitochondria were done from 20 x 106 cells (Mitochondria Isolation Kit for Cultured Cells, ThermoFisher).
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bioRxiv - Developmental Biology 2021Quote: ... The sample was then fixed with 4% paraformaldehyde/1XPBS solution for 20-30 and labelled using the Click-it OPP kit (Cat No C10457, Invitrogen) following the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 16000g for 20 min at 4°C and Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) was used to determine cleared lysate concentration ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 2 μg RNA in 20 μl reactions using High-Capacity Reverse Transcriptase kit (ABI, Thermo Fisher). Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Physiology 2022Quote: Cardiomyocyte MAO activity was assessed in 20 μg cell lysate using Amplex™ Red Monoamine Oxidase Assay Kit (ThermoFisher, A12214). p-tyramine is used as the substrate ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µL of each sample were withdrawn for total protein concentration determination (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific). Then ...
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bioRxiv - Microbiology 2023Quote: ... Cell monolayers were then loaded with the fluorescent substrate by adding 20 μl of 6X CCF4/AM solution (LiveBLAzer-FRET B/G Loading Kit, Invitrogen) containing 15 mM Probenecid (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... After 24 hours the cells were fixed with 4% paraformaldehyde in PBS for 20 minutes and then stained using the Click-iT 594 EdU kit and protocol (Invitrogen) with or without additional antibody staining and mounted using Prolong Gold mounting medium (Life Technologies) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in −20°C until total RNA was extracted using the PureLink RNA Mini Kit (Thermo Fisher Scientific; Cat no. 12183018A) according to the manufacturer’s instructions and quantified using NanoDrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... We visualized samples using 15 μL of LPS preparation on a 4%-20% Tris-glycine mini gel using the Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (ThermoFisher) (73).
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bioRxiv - Immunology 2023Quote: ... and stained at RT for 20 min with Live/Dead Fixable Aqua Dead Cell Stain Kit (Invitrogen; ThermoFisher; cat#L34957) diluted 1:500 ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20 ng RNA using the SuperScript™ VILO™ cDNA Synthesis Kit (#11754050; Thermo Fisher Scientific). Quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Bioengineering 2023Quote: ... The sample’s DNA was extracted using phenol-chloroform and kept at −20°C until applying the PicoGreen dsDNA assay kit (Invitrogen). Total sGAGs of the samples were obtained via papain (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... Slides were then incubated in 0.5% Triton X-100/PBS for 20 minutes at RT and EdU staining was performed using a Click-iT EdU Kit (Invitrogen) following the included protocol ...
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bioRxiv - Immunology 2023Quote: ... and stained at RT for 20 min with Live/Dead Fixable Aqua Dead Cell Stain Kit (Invitrogen; ThermoFisher; cat#L34957) diluted 1:500 ...
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bioRxiv - Microbiology 2020Quote: ... For calu3 infection 20 ug per ml TPCK trypsin (Thermo scientific) were added ...