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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... α-SC35 (described previously98, Mouse 1:1000), α-NIN (Institut curie, Human 1:200), anti-Arl13b (proteintech, Rabbit, 1:4500) α-Rabbit Alexa594 (ThermoFisher, 1:400), α-Mouse IgG1 Alexa488 (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901), Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The washing procedure was repeated and 50 μL of the buffer containing substrate (1-Step Ultra TMB ELISA Substrate, Thermo Scientific) was added to develop the colorimetric reaction for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... The peroxidase reaction was terminated by adding 50 μL of H2SO4 (1 M) before the final optical density (OD) was taken using an ELISA plate reader (Multiskan Go, Thermo Scientific) at the wavelength of 450 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... The washing procedure was repeated and 50 μL of the buffer containing substrate (1-Step Ultra TMB ELISA Substrate, Thermo Scientific) was added to develop the colorimetric reaction for 15 min ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 5 times with washing buffer and developed by addition of 100µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific) for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Immunology 2024Quote: ... plates were washed 3 times with PBST and added with 50 μL of 1-Step Ultra TMB-ELISA (ThermoFisher, Cat. #34028). Reactions were stopped by the addition of 50 μL of 2 M sulfuric acid ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Cell Biology 2021Quote: ... Hoechst 33528 (1-10 ug/mL) or Syto DeepRed (1:1000, ThermoFisher) were used for counterstaining DNA.
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bioRxiv - Physiology 2023Quote: ... we stained 10-micron colon sections for ZO-1 (Invitrogen, 1:100), EpCAM (Invitrogen ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides from each individual case and the GIS pooled standard or bridging sample (at least 1 per batch) were labeled using the TMT 10-plex kit (ThermoFisher 90406) for ROSMAP BA9 tissues ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated using High-capacity cDNA Reverse Transcription Kit in a 20 μl reaction volume and diluted 1:10 for qPCR analysis (Life Technologies). qPCR analysis was performed using 1 μl diluted cDNA with biological (2-3 ...
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bioRxiv - Cell Biology 2021Quote: Immature leaves collected from pSPCH::SPCH-GFP of 21-day-old plants were cross-linked using 1% formaldehyde under vacuum for 10 min according to the EZ-ChIP chromatin IP kit protocol (Thermo Scientific). After washing with phosphate-buffered saline solution ...
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bioRxiv - Microbiology 2021Quote: ... reverse transcription mixtures contained 1 µg or 10 µl RNA and High-Capacity RNA-to-cDNA reverse transcription kit (Applied Biosystems). qRT-PCR mixtures were assembled using 50 ng or 5 µl cDNA and PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... DNA extractions were diluted 1:10 and DNA concentration was measured using a Qubit dsDNA HS Assay Kit (Invitrogen, Waltham, USA). Depending on the concentration ...
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bioRxiv - Immunology 2023Quote: ... and 1 × 10^6 were processed using Click-iT EdU Pacific Blue Flow Cytometry Assay Kit (Thermo Fisher Scientific Cat. #C10418). The other 1 × 10^6 were subjected to anti-CD3 crosslinking and pERK staining as described below ...
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bioRxiv - Genetics 2021Quote: ... and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific) on an Applied Biosystems 7500 Real-time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: Human genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, USA) or QIAamp DNA Blood Mini Kit (QIAGEN ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated using a commercial kit (MagniSort Human CD4+ T Cell Enrichment; Affymetrix) and infected by incubation for 4 h at 37°C with 156,250 or 2,500 TCID50 (50% tissue culture infectious dose ...
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bioRxiv - Bioengineering 2022Quote: ... CD8+ T cells were positively enriched by Magnisort Human CD8+T cell positive selection Kit (Invitrogen). Nuclear fraction was isolated using NE-PER Nuclear and Cytoplasmic extraction kit (ThermoFisher) ...
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bioRxiv - Bioengineering 2019Quote: ... RNA from human cells was cleaned and collected utilizing an RNAqueous-Micro Kit (Ambion, cat. #AM1931) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Allogenic CD8+ were isolated by negative selection using Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen) and labeled with carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions with RNase T2 were done at room temperature for 10 minutes in 1× Structure Buffer (10 mM Tris pH 7, 100 mM KCl and 10 mM MgCl2; Ambion). Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5 ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks and nasal washes were diluted 10-fold in 1× minimum essential medium (MEM) (10% 10× minimal essential medium [Gibco] ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were immunostained according to standard protocols [21] using a primary rabbit anti-human antibody directed against zona occludens-1 (ZO-1; 1:100, Thermo-Fisher Scientific, UK) and a AF488-conjugated secondary goat anti-rabbit antibody (1:500 ...
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bioRxiv - Immunology 2020Quote: PBMC (1-2*10^6 cells) and pDC (1-4*10^4) were maintained for 16 h in RPMI1640+10% FBS+ 1% (PS) (Gibco Laboratories) in 96 well round bottom plates ...
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bioRxiv - Microbiology 2022Quote: ... 30 mM NaCl and 10 mM MgCl2) to a 10% sucrose concentration and ribosomes footprinted with 1 U/1×106 cells RNase I (Thermo Fisher), rotating overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated anti-human/mouse CD44 antibody (1:50 dilution, Molecular probes, A25528) was directly added into the cell culture medium of the cells to be stained ...
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bioRxiv - Genetics 2021Quote: ... donkey-anti-rabbit and mouse-anti-human IgG secondary antibodies (Molecular Probes, 1:1000), or NbALFA-Atto647 (NanoTag Biotechnologies ...
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bioRxiv - Developmental Biology 2021Quote: ... or of the rabbit anti-Human Rac1 polyclonal antibodies (1:40, Thermofisher PA1-091) were added in 1 % BSA-PBS and incubated O/N at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Systems Biology 2021Quote: ... followed by the secondary goat anti-human HRP-conjugated antibody (1:4000, A18805, Invitrogen). Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... anti-CD9 (1:500, mouse monoclonal anti-human CD9, clone MM2/57, Invitrogen, AHS0902). The membranes were then washed three times with 0.1% Tween 20 in PBS for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: Human primary fibroblasts FIB-1 and FIB-2 were cultured in DMEM medium (Gibco) supplemented with 10% FBS and Pen-Strep antibiotics ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 633-conjugated goat anti-human antibody (1:500; A21091 from Life Technologies); Alexa Fluor 555-conjugated goat anti-rabbit (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... Viable human tumor tissue pieces of ∼1-2mm3 were thawed in prewarmed DMEM (Gibco) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2019Quote: The human colorectal cancer cell line DLD-1 was cultured in DMEM (Life Technologies) supplemented with heat-inactivated 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Systems Biology 2020Quote: ... The human monocytic cell line THP-1 was maintained in RPMI 1640 medium (Gibco). All media were supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... and anti-human AlexaFluor 647 (1:500, 30 minutes, 4°C; Life Technologies, A21445), and resuspended in CellFix (1:10 in water ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...