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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... the slides were washed 3 times with PBS and incubated for 1h at room temperature with goat anti-rabbit AlexaFluor-568 (ThermoFisher, Invitrogen A-1101, 5ug/ml working solution). After washes with PBS ...
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bioRxiv - Genomics 2021Quote: ... 600,000 cells were plated in 6 well plates and transfected with 2 μg plasmid DNA using 6 μl Lipofectamine LTX reagent (Thermo Fisher). Medium was exchanged after 16 h and harvested 24 h later ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Neuroscience 2020Quote: Mice were physically restrained for 60 min in 50-ml polypropylene centrifuge tubes with 9 air holes of 3-mm diameter (339652, Thermo Fisher Scientific) (single exposure to restraint stress ...
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bioRxiv - Bioengineering 2019Quote: ... The slides were incubated overnight at 4°C with primary antibodies for anti -MMP-9 (Rabbit anti-Mouse) (Invitrogen, Carlsbad, CA), Rabbit anti-Mouse anti -MMP-2 (R & D Systems ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_10541045) for 3 days at 4°C followed by 2h in 1:800 anti-mouse FluoroNanogold (Life Technologies, A24920) at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The next day cells were transfected with a mixture of 4 ug plasmid DNA and a 1:3 ratio of PEI (1mg/mL) in Opti-Mem (Gibco). Forty-eight hours later ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were drawn after 0.5, 1, 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA plasmids were cotransfected with the packaging and envelope plasmids using Lipofectamine 3000 at a ratio of 4:3:1 (#L3000001, Thermofisher) into LentiX cells ...
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bioRxiv - Microbiology 2020Quote: The concentration of culture supernatants and MMP-9 were measured by Human MMP-9 ELISA Kit (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Mice were genotyped by PCR using forward primers 5’-ctgagcagagacccactgaaag-3’ and reverse primers 5’- ggatctggcttctgagtttgtgta-3’ and amplicons were ran in 6% TBE gels (Life Technologies, Carlsbad, CA).
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA). Viral supernatants were collected 48 hours post-transfection and filtered through a 0.45 μm Nalgene syringe filter SFCA (Whatman ...
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bioRxiv - Cell Biology 2020Quote: ... with one wash containing DAPI (1:50000, D3571, Invitrogen, Thermofisher), mounted with mouting media and stored in darkness at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... with one wash containing DAPI (1:50000, D3571, Invitrogen, Thermofisher), mounted with mouting media and stored in darkness at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Cells from one well of a 6-well plate were spun down and resuspended in 1mL TRIzol® (Life Technologies) for each respective sample ...
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bioRxiv - Cell Biology 2021Quote: 1.5×104 cells were seeded in one well of a 6-well plate (Falcon) with growth medium comprising of DMEM/F-12 (Gibco) with 20% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... ESCs in one well of a 6-well plate were transfected with 10 μg of expression vector using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: 1×105 N2A or HEK293T (ATCC) cells were seeded in one well of a 6-well plate in 2mL of DMEM complete media (Gibco) supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Molecular Biology 2019Quote: ... one cDNA fraction corresponding to 70–200 nt was gel purified after 6% TBE-urea gel electrophoresis (Thermo Fisher Scientific). After the final PCR step ...
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bioRxiv - Molecular Biology 2023Quote: ... from one full NGM agar plate or from one well from 6 well plates and total RNA was isolated with Trizol (Invitrogen). DNAse treatment was achieved using DNA-freeTM ...
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bioRxiv - Systems Biology 2024Quote: ... 1 million 293T STING-mNG or 0.5 million BJ1 seeded the same day in one well of a 6 well plate were transduced with 2ml of virus including 8µg/ml Protamine (Thermo Fisher). Cells were incubated with viral supernatant for 1-2 days and cells were then transferred in a fresh flask for selection ...
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bioRxiv - Developmental Biology 2020Quote: ... ES cells were passaged every 3–4 days using Accutase (Thermo Fisher) and used to passage 30 ...
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bioRxiv - Biochemistry 2020Quote: ... blotted for 3–4 s in a Vitrobot Mark IV (Thermo Fisher) at 15 °C and 100% humidity ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were grown for 3-4 days in Neurobasal medium (Thermo Fisher) supplemented with 2% FBS (HyClone) ...
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bioRxiv - Microbiology 2020Quote: ... at 4 °C for 3 h on a Labquake rotator (ThermoFisher Scientific). The beads were washed with 1 mL cold lysis/binding buffer four times at 300 g for 4 min at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... Stained cells were cytospun (Thermo Scientific Cytospin 4 1000rpm for 3 minutes) onto 1.5 thickness coverslips and mounted with ProLongTM Gold mounting medium (Molecular Probes #P36934 ...
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bioRxiv - Molecular Biology 2022Quote: ... iPSC-neurons were incubated with 3 µM Fluo-4 (Life Technologies #F14201) at 37°C for 20 min and imaged directly following washout on an LSM Zeiss 880 inverted confocal microscope using a 63×oil 1.4 numerical aperture objective and a 488-nm argon ion laser ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged every 3 to 4 days using Accutase™ (Gibco) for up to 10 passages.
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bioRxiv - Neuroscience 2024Quote: ... Invitrogen) and DiD (1,1’- Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt; Invitrogen) crystals were inserted under a stereo fluorescence microscope (MZ10 F ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged enzymatically every 3-4 days using 0.25% Trypsin (Gibco) diluted 1:3 in 1X PBS (Gibco) ...
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bioRxiv - Genetics 2022Quote: ... Cells (1-2.5 x106/mL) were then cultured at 37 °C ex vivo for 2-6 hours in RPMI (Gibco, 21875-034) containing 10% FCS (IGC Technical Services ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected using 1-2 µg of pMX-GFP or pMX-53BP1 vectors and 6 µL Lipofectamine 2000 (Thermo Scientific). The media was changed after 3 hours of incubation ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... and 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) (Invitrogen) in water for 1 hour at 60°C ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... one half of the Dicer-2 beads were incubated with 100 U RNase I (Ambion) in 100 µl IPP 150 at 25°C for 15 min on a thermomixer ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunolabeling with performed with mouse anti-B23 primary antibody (1:300; 2h) then detected with Alexa 488 donkey anti-mouse antibody (Invitrogen; 1:500; 1h), then further fixed with 4% paraformaldehyde in DEPC-PBS ...
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bioRxiv - Genomics 2020Quote: ... After incubation for 1h with a secondary fluorescently labelled antibody in BS (1:1000) the tissue was stained with 1:100 YOYO1 (Molecular Probes, Life Technologies) for 15 min and mounted with Vectashield Antifade Mounting Medium (H-1000 ...