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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for 6 Fluoro 1 3 benzodioxene 8 carbonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Cell nuclei were detected with TO-PRO-3 (1:400; Thermo Fisher Scientific). To analyze PNN structure and to detect synaptic proteins ...
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bioRxiv - Neuroscience 2021Quote: ... samples were then incubated with TO-PRO-3 (Thermo Fisher, T3605, 1:300), goat anti-rat Alexa Fluor 568 (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... A final incubation of 3 min with DAPI (1:1000, Thermo Fisher Scientific) was done ...
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bioRxiv - Microbiology 2021Quote: ... followed by DNA staining with 1 µM TOPRO-3 iodide (642/661; Invitrogen) and staining of the fungal hyphae with 100 μg/ml Fluorescent Brightener 28 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... slides were treated with DAPI (1:500, 3 min RT, ThermoFisher Scientific, D1306), washed briefly in PBS (2x) ...
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bioRxiv - Genetics 2021Quote: ... Nuclei were stained with TO-PRO-3 (Topro, Invitrogen, T3605; 1:3000 dilution).
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bioRxiv - Pathology 2021Quote: ... Membranes were blocked for 1 hour at RT with 3% BSA (Thermo Fisher) in tris-buffered saline with 0,1% Tween (TBS-T ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in a 3:1 mixture of IMDM (ThermoFisher Scientific Cat. 12440053) and Ham’s F12 (ThermoFisher Scientific Cat ...
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bioRxiv - Microbiology 2021Quote: ... and primer sequences (Table 1) on a QuantStudio 3 platform (Applied Biosystems, USA). The relative expression levels of target genes were plotted as fold change compared with untreated or negative controls ...
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bioRxiv - Immunology 2020Quote: ... 100 μl) mixed with Alum (1:3 v/v) (ThermoFisher, Waltham, MA, USA). HBc-S was used as a control ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 mM 3’-d-GTP [>99% purity for UTP and CTP (ThermoFisher); ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase,” and thereby to stabilize ...
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bioRxiv - Biophysics 2022Quote: ... Cells were split 1:10 every 3 days by using trypsin (Gibco, 25200072).
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bioRxiv - Cancer Biology 2022Quote: ... incubated for 3 minutes in Hoechst nuclear stain (1:1000 in H2O, Thermofisher), and mounted with Vectashield (VectorLaboratories ...
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bioRxiv - Synthetic Biology 2022Quote: ... for assembly into levels 1 and 3 or 10 U BpiI (Thermo Scientific) for assembly into levels 2 in 20 μl 1x T4 ligase buffer (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA and lipofectamine (1:3 w/v) were diluted in Opti-MEM (Gibco) and incubated at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and primary antibodies for 3 days: chicken anti-GFP (1:750, Thermo Fisher Scientific Cat# A10262 ...
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bioRxiv - Immunology 2023Quote: ... for 3 h and goat anti-rat AF 633 (1:200, Life technologies) for 1 h diluted in PBS containing 4 % BSA and 1 % goat serum at RT in a humidified dark chamber ...
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bioRxiv - Cell Biology 2023Quote: ... 0.05% Tween-20 in PBS along with 1:1000 To-Pro-3 (Invitrogen) for 2h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... species-specific secondary antibodies (Life Technologies; 1:300 dilution in 3% BSA/PBS). Images were collected on a Zeiss Axioimager.D2 upright microscope using a 63x/Plan-neofluar objective and captured using a Coolsnap HQ2 camera (Photometrics ...
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bioRxiv - Biophysics 2023Quote: ... NucGreen™ Dead 488 (Invitrogen™, 1 drop per 3 ml in PBS); Alexa 647 Phalloidin (Invitrogen™ at 1/500) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 U/ml EPO (Zyrop 4000 IU injection) and 1× Pen-Strep (Gibco) as per established protocols ...
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bioRxiv - Cell Biology 2023Quote: ... INS-1 832/3 cells were transfected with 40 nM of Control (Ambion Silencer Select Negative Control #1 siRNA ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were maintained in E-medium (3:1 ratio of DMEM:F12 (Thermofisher, 21765029) medium supplemented with 10 % FBS and 1 % PS ...
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bioRxiv - Microbiology 2024Quote: ... or streptavidin-HRP (1:1000 dilution, Fisher Scientific, Novex Cat# 43-423-3) to ensure that biotinylated and bait proteins were present ...
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bioRxiv - Microbiology 2020Quote: ... 5-(and-6)-carboxyfluorescein (FAM) and 5-(and-6)-carboxytetramethylrhodamine (TAMRA) were obtained from Invitrogen™ ...
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bioRxiv - Plant Biology 2023Quote: ... 6 uL of 0.5 M EGTA (bioWORLD) and 6 uL of 0.5 M EDTA (Invitrogen) were added to quench the reaction at 65 °C for 10min ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug dilution series (1:3) were prepared in PDAC organoid culture medium containing 1 μg/ml Hoechst (Invitrogen) and 1 μg/ml PI (Sigma) ...
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bioRxiv - Physiology 2022Quote: ... Plasmid DNA was mixed with PEI (1 mg/ml) at 1:3 in Opti-MEM medium (Gibco, 11058021) and added to cells after short incubation ...
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bioRxiv - Genetics 2022Quote: Calu-3 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/F12 (1:1) GlutaMAX™ (ThermoFisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the tumoroids in a 1:3 and 1:10 effector:target (E:T) ratio in RPMI 1640 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2019Quote: ... at 4°C for 1-3 days followed by secondary antibody (1:500, Thermo Fisher Scientific #A-21206) overnight at 4°C.
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 3 μg mRNA at a mass ratio of 1:1 using Lipofectamine MessengerMAX (Invitrogen) according to the manufacturer’s guide ...
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bioRxiv - Neuroscience 2024Quote: ... stained with TO-PRO-3 diluted 1:1000 in DPBS and 1 µM Hoechst 33342 (Thermo Fisher 62249) nuclear counterstain for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... The unbound Rep-X was washed off simultaneously and the unwinding reaction was initiated by adding the unwinding buffer (10 mM Tris-HCl, pH 8, 100 mM KCl, 2.5 mM MgCl2, 1 mM ATP [ThermoFisher Scientific], 10 % glycerol, 1 % BSA). For characterization of the maiden folded GQ conformation ...
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bioRxiv - Immunology 2023Quote: ... were distributed into four wells of a 24-well plate and activated for 8 days by adding prewashed anti-CD3/CD28 Dynabeads at a 1:1 bead-to-cell ratio (Invitrogen/Thermo Fisher Scientific, Massachusetts, USA) and human rIL-2 (30 U/ml ...
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bioRxiv - Microbiology 2021Quote: ... was accomplished by reacting the respective oligosaccharide glycamines with Lissamine rhodamine B sulfonyl chloride (LRSC) (Acros Organics, Belgium, USA) as described previously (64) ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by Poly(A) tailing procedure and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion). pCMV-ancBE4max (pCMV_AncBE4max was a gift from David Liu (Addgene plasmid # 112094 ...
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bioRxiv - Microbiology 2020Quote: Approximately 5 μg of BAC DNA was linearized with NotI and full-length RNA was obtained by in vitro transcription with T7 RNA polymerase followed by lithium chloride precipitation according to the manufacturer’s protocol (mMessage-mMachine T7 Kit; Ambion). 5 μg of RNA was electroporated into 5×106 BHK-21 cells using the Amaxa nucleofector 2b (program A-031 ...
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bioRxiv - Immunology 2022Quote: ... for 5 minutes and pellets were resuspended in Ammonium-Chloride-Potassium (ACK) lysing buffer (Thermo Fisher Scientific, Catalog # A1049201) to lyse RBC (erythrocytes removal performed at R.T ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed gradually into 2X SSC buffer (0.3 M sodium chloride, 30 mM sodium citrate or AM9763, Invitrogen), and then gradually from SSC to PBT ...
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bioRxiv - Microbiology 2023Quote: ... Red blood cells (RBCs) were then lysed by resuspending cells in ammonium-chloride-potassium (ACK) lysis buffer (Thermo Fisher) for 2 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C (for P. miniata) or 20C (for P. regularis) in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Pharmacology and Toxicology 2022Quote: HEK293T cells were seeded at the density of 106 cells/well in a 6-well plate and were grown overnight in complete DMEM/F12 1:1 Glutamax ™ (Thermofisher, UK). On the next day ...
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bioRxiv - Zoology 2023Quote: ... stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000 dilution) and Alexa Fluor-conjugated donkey-anti-mouse (Molecular Probes, 1:1000), in PBST at room temperature for 2 hours ...
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bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
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bioRxiv - Microbiology 2020Quote: ... University of Bristol) were grown in DMEM supplemented with 8% FCS and 8% (v/v) tryptose phosphate broth (Life technologies). Penicillin and streptomycin (90 IU/ml ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...