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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for 1 Ethylbenz cd indol 2 1H ylidene malononitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... and ADAM9 siRNA-2 (AD9-2) (Ambion 4390826). Antibodies used in this study include rabbit anti-ADAM9 (CST 4151 ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche], 2 U/mL DNase I [Life Technologies] and Dispase II [Roche]). The digested brain parenchyma ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) and 1% (v/v) penicillin/streptomycin (P/S) (all from Thermo Fisher Scientific, Waltham, MA, USA). Then ...
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bioRxiv - Biochemistry 2024Quote: ... 150 mM KCl, 1 mM EDTA ethylenediaminetetraacetic acid [EDTA], 2 mM MgCl2, 0.5 mM ATP, 0.02% Brij-35 [ThermoFisher Scientific], 5 mM 2-ME, pH 8.0) supplemented with 50 U ml-1 Benzonase (Merck-Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific, for the 1h dataset, and Ultimate 3000 RSLCnano and Orbitrap Exploris 480, Thermo Fisher Scientific, for the 24h dataset) using a 120-min gradient ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 (Thermofisher). A reverse transfection approach was used to deplete AurkA and CEP135 ...
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bioRxiv - Systems Biology 2023Quote: ... 2% (ThermoFisher). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic) using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Macrophages were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
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bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
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bioRxiv - Biophysics 2020Quote: All the in vitro transcription experiments were performed in NEB’s transcription buffer (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine) with 1 mM NTPs (R1481, Thermo Scientific), 22 wt% glycerol ...
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml or Dynabeads Mouse T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Cell Biology 2020Quote: ... guide RNA plasmid and a plasmid expressing SpCas9 at a ratio of 2:1:1 (total 12ug) using Neon electroporator (Life Technologies) and a 30ms ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were transiently transfected with HA-Flag-GHRHR-Rluc8 or HA-Flag-SV1-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using Lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Microbiology 2021Quote: ... fixed and permeabilized specimens were stained for 1 hour at room temperature by adding PermQuench buffer containing 1 μg/mL Hoechst 33342 and 2 μg mL-1 CellMask™ Deep Red plasma membrane stain (H32721, Invitrogen). After staining ...
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bioRxiv - Neuroscience 2020Quote: ... with 5% DMSO for at least 2 nights at room temperature with the following reagents: (1) 1:20 AlexaFluor 594 phalloidin (ThermoFisher #A12381) (Figure 3F ...
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bioRxiv - Neuroscience 2021Quote: ... Striatum slices were further stained for 1-2 h at RT with NeuroTrace 435/455 (1:100 diluted in PBS, Invitrogen, #N21479), a fluorescent Nissl staining ...
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bioRxiv - Cell Biology 2021Quote: ... Cardiac cells were subsequently thawed in RPMI+B27 media (RPMI 1640 supplemented with 2% B-27 Supplement minus vitamin A, 1% GlutaMAX and 1% Penicillin/Streptomycin- All from ThermoFisher Scientific) with Rock inhibitor (Selleck Chemicals ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transiently transfected with HA-GLP-1R-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Immunology 2020Quote: ... monocytes were plated in low-serum media (1% FBS) for 1-2 hours in 12-well Nunc-coated plates (ThermoFisher Scientific) to promote adherence ...
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bioRxiv - Biochemistry 2020Quote: The cDNA coding for SARS CoV-2 Nsp1 1-180 Nsp2 1-19 8His was synthesised by GeneArt (Thermo Fisher Scientific) adding NcoI and NotI sites at the 5’ and 3’ ends ...
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bioRxiv - Plant Biology 2022Quote: ... To each sample 0.7 µL of reverse transcription mix (8:2:1:1 of Superscript IV 5X Buffer, 100 mM DTT, RNase Inhibitor, Superscript IV; ThermoFisher Scientific) was added ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Developmental Biology 2022Quote: ... tissues were incubated with 1:200 anti-rat IgG 647 for 2 h at room temperature with 1:500 phalloidin TRITC (Fisher Scientific). Then ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were washed in block six times for 1 hour and incubated in secondary antibodies conjugated to fluorescent dyes diluted 1:250 in block with added 4’,6-diamidino-2-phenylindole (DAPI; 1:500 dilution of 1 mg/ml stock, Thermo Fisher) for 2 nights at 4°C.
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bioRxiv - Neuroscience 2024Quote: ... To visualize Gi-DREADD-mCherry expression in rCA2 of injected mice sections were stained with 1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... In order to visualize AAV-retro positive cells sections were stained for mCherry (1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... A subset of sections was stained for calbindin+ cells (1⁰ rabbit anti-calbindin (Swant #CB38) 1:500 + 2⁰ anti-rabbit-633 (Invitrogen #A21071) 1:500) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated in the dark at room temperature for 2 hours with 1:1000 DAPI and 1:500 AlexaFluor secondary antibodies (Thermo Fisher) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2-hour incubation with secondary antibody (1:500 in 1% BSA in PBST; AF488 anti-rat, Invitrogen, A-11006) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: The datasets of ULK1C (2:1:1) core and the ULK1C:PI3KC3-C1 mixture were recorded at a 300 kV Titan Krios microscope (Thermo Fisher Scientific) equipped with X-FEG and energy filter set to a width of 20 eV ...
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bioRxiv - Biochemistry 2023Quote: ... fresh P3 virus from γ-TuRC-GFP and γ-TuSC were mixed in a 1:1 ratio and used to infect 1.8-2 L of High five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 30 mL of the γ-TuRC-GFP and γ-TuSC virus mixture as above ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Neuroscience 2023Quote: ... were selected and processed as described above with primary antibody Rabbit anti-Cox-2 (1:1k, Cayman Chem, 160123) and secondary antibody AF555 donkey anti-rabbit (1:200, ThermoFisher, A31572).
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 ml of 56°C warm gel solution (1 volume RPMI/20% FBS, 1 volume 2x HBSS, 2 volumes 2.5% agarose in water (ultrapure agarose, Invitrogen, #16500-100)) were cast per 35 mm dish and allowed to solidify ...