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Citations for Thermo Fisher :
1501 - 1550 of 7701 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were transfected to HEK cells in OPTI-MEM using lipofectamine 2000 (Invitrogen) at 70-80% confluency.
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bioRxiv - Neuroscience 2022Quote: ... HEK-293FT cells were dissociated by incubating for 5 min with Trypsin-EDTA (Gibco) at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cas9 Nuclease V3 and transfected into HEK T-REx cells using Lipofectamine RNAiMAX (Invitrogen) according to the IDT user guide ...
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bioRxiv - Microbiology 2023Quote: ... Hela and HEK 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplemented with 10% foetal calf serum (FCS ...
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bioRxiv - Cell Biology 2022Quote: HeLa and HEK 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... HEK-293F cells were transiently transfected by the use of 293fectin (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... HEK-293T cells were washed with Phosphate-Buffered Saline (PBS) (Thermo Fisher Scientific, AM9625) and trypsinized (Trypsin EDTA 1X 0.25% Trypsin/ 2.21mM EDTA in HBSS without sodium bicarbonate ...
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bioRxiv - Neuroscience 2023Quote: ... and transfected on HEK-294 cells with Lipofectamine 3000 (Thermo Fisher Scientific, L3000-008) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: HEK 293T (ATCC: CRL-3216) were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2023Quote: ... HEK 283 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, 11965-084) and supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2023Quote: Proteins were expressed recombinantly in-house using the HEK 293Freestyle expression system (Life Technologies). Cells were transfected using PEI Max (Polysciences ...
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bioRxiv - Immunology 2023Quote: Expi293F HEK cells were cultured in suspension in Expi293 expression medium (Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral particles were produced in HEK 293FT cells co-transfected via Lipofectamine 2000 (Invitrogen) with the shRNA vector and Virapower packaging plasmids (pMDLg/pRRE ...
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bioRxiv - Immunology 2024Quote: ... and HEK-293T (ATCC) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with 10 % FBS and 100 μg / mL penicillin / streptomycin ...
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bioRxiv - Biochemistry 2023Quote: HEK 293FT cells were grown in DMEM with high glucose (Gibco, Thermo Fisher #11965092) supplemented with 10% fetal bovine serum (VWR #97068-085) ...
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bioRxiv - Biochemistry 2023Quote: HEK 293FT cells were grown in DMEM with high glucose (Gibco, Thermo Fisher #11965092) supplemented with 10% fetal bovine serum (VWR #97068-085) ...
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bioRxiv - Neuroscience 2024Quote: Clontech’s HEK 293T cell line were cultured in D-MEM high glucose medium (Invitrogen), containing 4.5 g/L D-glucose ...
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bioRxiv - Microbiology 2024Quote: HEK TER cells were described previously and cultured in Minimum Essential Media (MEM, Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... and pRL-TK into HEK-293T cells for 24 h using Lipofectamine 3000 (Invitrogen) reagent ...
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bioRxiv - Neuroscience 2024Quote: ... Adherent HEK 293T cells were grown in Dulbecco’s modified Eagle’s medium + GlutaMAX (Thermo Fisher) supplemented with 10% fetal bovine serum (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Microbiology 2020Quote: ... All constructs were transfected into Expi293 cells using ExpiFectamine(tm) 293 Transfection Kit (Thermo Fisher). Antibodies were purified by immobilized metal chelate affinity chromatography using nickel-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Molecular Biology 2020Quote: ... and stable FlpIn 293 T-REx cell lines were maintained with hygromycin (Life Technologies, 10687010) at 2ug/ml ...
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bioRxiv - Bioengineering 2022Quote: ... Suspension-adapted HEK293F cells were maintained in serum-free Free Style 293 Expression Medium (Gibco). On the day of transfection ...
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bioRxiv - Synthetic Biology 2019Quote: Gene circuits were introduced into Flp-In 293 cells (Thermo Fisher Scientific catalog number R75007) using lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... suspension adapted HEK293GnTI- cells growing in freestyle 293 expression media supplemented with 2 % FBS (Gibco), 2 mM Glutamine (Gibco ...
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bioRxiv - Synthetic Biology 2021Quote: FreeStyle 293-F and FreeStyle CHO-S cell lines were obtained from Invitrogen (Carlsbad, CA) and cultured in FreeStyle 293 Expression Medium and complete FreeStyle CHO Expression Medium (Invitrogen) ...
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bioRxiv - Synthetic Biology 2021Quote: ... CA) and cultured in FreeStyle 293 Expression Medium and complete FreeStyle CHO Expression Medium (Invitrogen), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant IgG (XBA14 and XBA18) was produced in FreeStyle 293 cells (Thermo Fisher Scientific), and purified by Protein A-Sepharose (Cytiva ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 μg/ml streptomycin or the GripTite 293 MSR Cell Line (Thermo Fisher Scientific) (HEK-GT ...
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bioRxiv - Bioengineering 2022Quote: ... was expressed in an Expi293F cell system with an ExpiFectamine 293 Transfection Kit (ThermoFisher, USA) according to the manufacturer’s protocol and purified as described previously 45.The ACE2 or DnbALFA proteins were labeled by CF®640R succinimidyl ester dye (Biotium ...
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bioRxiv - Microbiology 2021Quote: ... was transfected into 293 LTV cells for lentivirus production using Lipofectamine 2000 (Thermo Fisher Scientific). The supernatant was collected 48 hours post transfection and clarified by centrifugation (3500 rcf ...
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bioRxiv - Molecular Biology 2019Quote: ... All experiments were performed in the Flp-In T-REx 293 cell line (Thermo Scientific). The mCherry and mEGFP versions of RFP and GFP are used throughout this manuscript ...
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bioRxiv - Cancer Biology 2019Quote: The Flp-In™ T-REx™ 293 Cell Line was procured from Thermo Fisher Scientific (R78007 ...
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bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with 25µL of odorant solution diluted in CD-293 (Gibco 11913-019) containing 30 µM CuCl2 (Sigma-Aldrich C-6641 ...
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bioRxiv - Genetics 2021Quote: ... Flp-In T-REx 293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) and supplemented with 10% Fetalgro® Bovine Growth Serum (BGS) ...
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bioRxiv - Genomics 2020Quote: ... Flp-In T-REx 293 cells were also supplemented with 100 μg/ml Zeocin (Invitrogen). Cells were passaged every 2-4 days ...
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bioRxiv - Immunology 2020Quote: ... Cells were maintained at 37°C and 6% CO2 FreeStyle 293 Expression Medium (Thermo Fisher) and 0.2% Penicillin/Streptomycin 7 days post transfection ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The transfection mix was replaced after 6 hours by 293 SFM II suspension medium (Invitrogen, 11686029 ...
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bioRxiv - Microbiology 2023Quote: ... Expi293 cells were transfected with S-6P-NanoLuc expression plasmids using ExpiFectamine 293 (ThermoFisher Scientific). Four days later ...
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bioRxiv - Biochemistry 2023Quote: ... Baculovirus in the supernatant was pelleted and resuspended in FreeStyle 293 Expression Medium (Life Technologies) supplemented with 2% Fetal Bovine Serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... MCF7 (obtained from ATCC; cat# HTB-22) and 293 TREx cells (ThermoFisher, cat# R780-07) were cultured in Dulbecco’s modified eagle medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... All adenovirus vectors were produced in the T-REx-293 cell line (Thermo Fisher Scientific), which allows for transcriptional repression of the vaccine antigens during vector production ...
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bioRxiv - Immunology 2023Quote: ... All adenovirus vectors were produced in the T-REx-293 cell line (Thermo Fisher Scientific), which allows for transcriptional repression of the vaccine antigens during vector production ...
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bioRxiv - Bioengineering 2024Quote: ... according to the manufacturer’s instructions and cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific) in a humidified incubator with 8% CO2 at 37°C and 125 rpm ...
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bioRxiv - Cancer Biology 2021Quote: Single live cell suspensions were washed with 1% FCS in PBS and resuspended in flow cytometry buffer (2% FCS and 1 mM ethylenediaminetetraacetic acid (EDTA; Thermo Fisher #AM9260G) in PBS).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Random primer (fc 50 ng/µl) and RNase Inhibitor (fc 1 unit/µl) in 1X First Strand synthesis buffer (Invitrogen/Life Technologies). Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays ...
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bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...