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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Recombinant Mouse Mcpt1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 6 µg RNA was used and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant chimeric antibodies were produced using the Expi293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated recombinant toxins were captured using streptavidin-coated Dynabeads (Invitrogen, M-280). After washing with PBS containing 0.1% (v/v ...
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bioRxiv - Physiology 2023Quote: ... Recombinant TEVp was purified as previously described1 or was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... were coated with 50 ng of recombinant Gag/well (35) in 1xPBS (Gibco) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The recombinant NS1 gene was cloned into the pTRC–His2c expression vector (Invitrogen) and expressed in the BirA-enzyme containing E ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and incubated with recombinant enzymes TrypLE (Thermo Fisher) for 10 minutes at 37°C for single cell dissociation ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 ng/ml recombinant noggin (Thermo Fisher Scientific, cat# 120-10C-250UG). The 3dGRO™ human ISO72 colorectal cancer tumoroid line was purchased from Millipore Sigma (Burlington ...
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bioRxiv - Molecular Biology 2020Quote: ... these PER2 cDNAs were sub-cloned into various tagged vectors including pcDNA3.0.HA (Thermo Fisher Scientific, Waltham, MA), 3XFLAG-CMV-7.1-2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... FAM fluorescent tagged TaqMan® copy number assay probes detecting the ETNK1 gene (Hs01860263_cn) (Life Technologies, Carlsbad, CA) was used to quantify the copy number of 12p ...
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bioRxiv - Cell Biology 2020Quote: Overexpression of mCherry-tagged actin constructs in HEK293T cells was carried out using Lipofectamine 2000 transfection reagent (ThermoFisher) at a ratio of 1:2.5 ...
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bioRxiv - Bioengineering 2021Quote: ... The Arabidopsis eEF-1 α gene (Assay ID. No. At02337969_g1) tagged with VIC dye (Life Technologies, Carlsbad, CA) was added in each well as an internal control ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged TFs assayed in PBMs were detected either with Alexa488-conjugated anti-GST antibody (Invitrogen A-11131), or with a TF-specific PCRP antibody ...
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bioRxiv - Biophysics 2021Quote: ... 0.2 µg DNA encoding GFP-tagged T-Plastin (WT or mutant) and 0.25 µL Lipofectamine2000 (Thermo Fisher Scientific), mixed in 20 µL OptiMEM (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The expressed C-terminal 6xHis-tagged Nluc was Ni-NTA purified using HisPurTMNi-NTA Resin (ThermoFisher scientific, #88221) as per the HisPurTMNi-NTA Resin protocol ...
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bioRxiv - Cell Biology 2021Quote: ... AF555-and AF647-tagged secondary antibodies (donkey; 1:1000, cat. num. A-21208, A-31572, A-31571, ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: 6-dpf zebrafish larvae were gavaged with Alexa Fluor 488-tagged dextran 10,000MW (fDex) solution (Molecular Probes, USA) or purified mCherry solution (prepared in the laboratory ...
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bioRxiv - Molecular Biology 2022Quote: MCP-tagged SARS-CoV-2 expression plasmids for the MS2 tethering assay were generated by Gateway Assembly (ThermoFisher). SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Biochemistry 2019Quote: ... anti V5-HRP conjugate (to detect mSCARLET and other V5 tagged fluorescent proteins, catalog number 46-0708, Invitrogen), rabbit polyclonal anti TAL1 IgG (catalog number A305-300A ...
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
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bioRxiv - Biochemistry 2021Quote: Flag-tagged UCH37 (WT, C88A, ΔCTD, ΔCTD/C88A, EWI, EWI/C88A) were cloned into pcDNA5/FRT/TO (Invitrogen). Stable cell lines that inducibly express these proteins were constructed from Flp-InTM T-RExTM 293 Cells following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM maltose.) SKD3-MBP fusion protein was cleaved with a 6His-tagged AcTEV™ protease (Thermo Fisher) at 4 °C for 96 h in the amylose elution buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Inserts were then subcloned into pCS2+ N’ HA tagged vectors that had been converted into Gateway® (Invitrogen) destination vectors ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:5000 dilution and endogenous LC3 was tagged with an LC3B/MAP1LC3B primary antibody (ThermoFisher, L10382) used at a dilution of 1:500 for 72 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HA-glmS-tagged parasite lines were coated on diagnostic slides (Epredia; Thermo Fisher Scientific; Waltham, US) and air-dried ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
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bioRxiv - Bioengineering 2024Quote: ... cells were either transduced to include a fluorescent reporter or fluorescently tagged using Hoechst (NucBlue Live ReadyProbes, Invitrogen) or general membrane cell tracker (Cellvue Claret Far Red ...
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bioRxiv - Biochemistry 2024Quote: Construct containing Avi-tagged HormR/GAIN domains of ADGRL3 was expressed in High Five cells (Thermo Fisher, B85502) grown in Insect-Xpress medium (Lonza ...
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bioRxiv - Evolutionary Biology 2023Quote: Lamprey embryos were injected with MYC-tagged lamprey (pou5) or Xenopus (pou5f3.1, pou5f3.2, pou5f3.3) mRNA and a FITC dextran tracer (ThermoFisher AAJ6360622) into one blastomere at the two-cell stage at the same concentration used for injections in Xenopus ...
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bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
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bioRxiv - Biochemistry 2022Quote: Cloning of full-length N-terminal strepII-tagged hItln1 into the pcDNA4/myc-HisA vector backbone (Life Technologies) was described previously (14) ...
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bioRxiv - Cell Biology 2023Quote: ... To visualise the cell surface with Alexa488-tagged wheat germ agglutin (Thermo Fisher Scientific, UK, 1:2000 dilution) an incubation was done prior to permeabilization ...
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bioRxiv - Molecular Biology 2023Quote: ... Genes encoding tagged and untagged SOSIP.664 Env homotrimers were expressed by transient transfection of Expi293 cells (ThermoFisher). Env heterotrimers were purified from co-transfections involving a 20:1 expression plasmid DNA ratio of untagged to tagged Env constructs ...
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bioRxiv - Cell Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5′ ends were synthesized by Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged β-catenin variants were expressed in transient transfection assays using the pCDNA3.1 vector (Thermo Fisher Scientific). A plasmid expressing human β-catenin containing a S33Y mutation (pCDNA3-S33Y ...
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bioRxiv - Molecular Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5’ ends were synthesized by Thermo Scientific, while those labeled with ATTO565 at the 5’ end was synthesized by Sigma Aldrich ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
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bioRxiv - Bioengineering 2024Quote: GFP-HUVECs were added to well 1 and GFP-HUVECs co-tagged with Hoechst (NucBlue Live ReadyProbes, Invitrogen) were added to wells 2-4 ...
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bioRxiv - Cell Biology 2023Quote: ... strep tagged APC/C was pre-incubated on ice with streptavidin conjugated Alexa Fluor 700 (ThermoFisher Scientific S21383) for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with plasmids encoding fluorescence-tagged PARP2 or XRCC1 using Lipofectamine 2000 (Invitrogen, Cat. 11668019) or Lonza 4D-Nucleofector™ X ...