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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Recombinant Mouse Il1f6 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the cells were transfected with 1μg/well of the HA-tagged Fpn plasmid (Vector Builder, VB220407-1185gaa, Supplemental Figure 1) using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000001).
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bioRxiv - Biochemistry 2023Quote: ... Cells were then again incubated with p65 primary antibody (1:1000) overnight and successively incubated with secondary antibody tagged with Alexa Fluor IgG 555 (#A32732, Thermo Fisher Scientific) as per abovementioned protocol ...
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bioRxiv - Bioengineering 2024Quote: ... and human and mouse DKK3 (hDKK3: AA 1-350, mDKK3: AA 1-349) triple tagged with Fc-Myc-6xHis were transfected into Expi293FTM (Thermo Fisher Scientific) cells at a density of 3 × 106 viable cells/mL using ExpiFectamineTM (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... mice were inoculated with 500,000 DIPG17-luciferase tagged cells in 1:1 suspension (200 μl total) with full media and MatrigelTM (Fisher Scientific CB-40234) via subcutaneous injection in the left flanks ...
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bioRxiv - Systems Biology 2023Quote: ... ABE8e was expressed as an 8xHis tagged protein from a rhamnose-inducible promoter in BL21-Star DE3 cells with low RNase activity (Thermo Fisher Scientific). At an OD600 of ∼0.8 ...
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bioRxiv - Cell Biology 2023Quote: ... HaCaT cells were transfected with a 10 nM duplex of crRNA and Alt-R CRISPR-Cas9 tracrRNA tagged with ATTO 550 (Integrated DNA Technologies) using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the brain sections were washed for 5 min x3 in 0.1% Tween 20/PBS and incubated in fluorescence tagged secondary antibodies for 2 hours at RT: Alexa Fluor 594 anti-rabbit (1:500, ThermoFisher Scientific, A11037), Alexa Fluor 488 anti-mouse IgG1 (1:500 ...
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bioRxiv - Biophysics 2024Quote: RBL-2H3 cells stably expressing clathrin light chain A tagged with green fluorescent proteins were cultured in minimum essential medium (MEM) (Invitrogen 11095-09) with 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... 1X FLAG (DYKDDDDK)-tagged CLCC1, TMEM41B (DNASU, HsCD00829148), and VMP1 (DNASU, HsCD00080545) were cloned using the Gibson assembly and the Gateway system (Thermo Fisher, 11791020) in a pLenti-CMV-Hygro vector (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids expressing GST-tagged Spt6p were produced by inserting the Spt6p(1223-1451) coding sequence into the pDEST15 vector (Thermo Fisher Scientific). Mutations in the tSH2 domain were introduced using site-directed mutagenesis (Sdano et al ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ZO-1 (mouse monoclonal, Invitrogen 33-9100, 1:500), rabbit anti-aPKC (rabbit polyclonal ...
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bioRxiv - Immunology 2020Quote: ... mouse IFNβ (PBL Biomedical Laboratories) and mouse CXCL10 ELISA (ThermoFisher; BMS6018MST) were obtained from commercial sources.
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bioRxiv - Developmental Biology 2023Quote: ... Mouse aggregation medium (mouse AM) consisted of 1x DMEM (GIBCO-41965) supplemented with 20% FBS (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel droplets were overlaid with recombinant organoid medium (Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and 50μl Pierce recombinant protein A/G with peroxidase (Thermo Fisher catalog no: 32490) added at 1:10,000 in PBS-T with 1% milk as a secondary and incubated 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: Clones of pFastBac1 constructs were used to generate recombinant bacmids in DH10Bac following the manufacturers protocol (ThermoFisher). Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus) ...
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bioRxiv - Genomics 2020Quote: ... cells were serum-starved (with 0.3% SCS) for 72 hours prior to induction of gene expression with 0.1µg/mL recombinant EGF (Gibco) or PBS for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: Recombinant PfbA (rPfbA) or BSA was coated onto 0.5-µm-diameter fluorescent beads (FluoroSphere, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 ng of poly(dI-dC) were used for 100 ng of recombinant ERα (ThermoFisher, RP-310) and 1 µg for 10 µg of nuclear extracts ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit IgG antibodies (1:500 for IF) and recombinant human BMP2 (#PHC7145) were from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: For recombinant protein expression in Pichia pastoris GS115 (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs of IF1 ...
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bioRxiv - Biophysics 2021Quote: ... and 10 μg/mL Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 555 Conjugate (CTSB, Fisher Scientific) to label GM1 as the acceptor ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant forms of SyAM in culture supernatant were isolated by protein A affinity resins (MabCapture, ThermoFisher Scientific) using Pierce gentle Ag/Ab elution buffer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel droplets were overlaid with recombinant organoid medium: Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Immunology 2022Quote: Recombinant gH/gL proteins were biotinylated using the EZ-Link NHS-PEG4-Biotin Kit (ThermoFisher, catalog #21330) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Microbiology 2020Quote: The recombinant proteins were purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA). The lysate supernatant from the 50 ml culture was mixed with 300 μl Ni-NTA beads and incubated overnight at 4° in the lysis buffer supplemented with 5 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were prepared in Spodoptera frugiperda (Sf9) cells using the Bac-to-Bac system (Life Technologies). Recombinant protein complexes were expressed in Spodoptera frugiperda by co-infection of single baculoviruses ...
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bioRxiv - Neuroscience 2021Quote: ... was added to each sample (4μl 5X First-Strand Buffer, 1μl 0.1 M DTT, 1μl RNaseOUT Recombinant RNase Inhibitor (Invitrogen; 10777019), 1μl of SuperScript III RT (200 units/μl) ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Pathology 2020Quote: ... A 1ug/mL mixture of 50μL per well containing recombinant protein in carbonate buffer (0.1M Na2CO3 0.1M NaHCO3 pH 9.3) was used to coat MaxiSorp (ThermoFisher) 96-well plates overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... fibroblast outgrowths were dissociated and split 1:4 using a recombinant trypsin-like enzyme (TrypLE Select, Invitrogen). Additional fibroblast preparations were commercially available ...
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... The recombinant Fab was purified from culture supernatant using an anti-CH1 CaptureSelect column (Thermo Fisher Scientific). For the RBD/COV2-2196 complex ...
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bioRxiv - Immunology 2021Quote: ... The protein was transiently expressed as a soluble recombinant protein in mammalian Expi293 cells (Thermo Fisher Scientific). The secreted protein self -assembles into a protein nanoparticle ...
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bioRxiv - Microbiology 2022Quote: ... The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific) using bovine serum albumin (BSA ...