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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Recombinant Mouse ALCAM Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was measured through the Bicinchoninic Acid protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™ 23225). Equal amounts of proteins extracts (20-30 μg for mitochondrial samples or 40 μg for total kidney homogenates ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein in brain tissue was determined using a protein assay kit (Pierce™ BCA Protein Assay Kit; ThermoFisher, 23225) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... Protein extraction was performed as described previously37 and protein concentration was determined using Pierce BCA protein assay kit (Thermo scientific).
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant containing the protein extract was collected and the total protein concentration was determined (BCA Protein Assay Kit, Thermofisher). Samples were reduced with DTT (15 mM ...
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bioRxiv - Biochemistry 2023Quote: ... Dried proteins were reconstituted in 0.2 % SLS and the amount of proteins was determined by bicinchoninic acid protein assay (Thermo Scientific). For tryptic digestion 50 μg protein was incubated in 0.5% SLS and 1 μg of trypsin (Serva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Protein purity and concentration were determined using Coomassie Imperial Protein Stain and Pierce bicinchoninic acid protein assay (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Total protein quantification was determined using Pierce™ BCA Protein Assay Kit (Thermo Scientific Pierce™ BCA Protein Assay Kit) and samples were stored at -80°C until use.
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bioRxiv - Pathology 2023Quote: ... aortic protein lysates were prepared in RIPA lysis buffer and protein concentration was measured using the BCA Gold protein assay (ThermoFisher). Equal protein amounts (25μg ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysates were sonicated and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, cat.no. 23225) for subsequent analyses.
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bioRxiv - Microbiology 2024Quote: ... Dried proteins were reconstituted in 0.2% SLS and the amount of proteins was determined by bicinchoninic acid protein assay (Thermo Scientific). For tryptic digestion 50 µg protein was incubated in 0.5% SLS and 1 µg of trypsin (Serva ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel droplets were overlaid with recombinant organoid medium (Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: Clones of pFastBac1 constructs were used to generate recombinant bacmids in DH10Bac following the manufacturers protocol (ThermoFisher). Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus) ...
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bioRxiv - Genomics 2020Quote: ... cells were serum-starved (with 0.3% SCS) for 72 hours prior to induction of gene expression with 0.1µg/mL recombinant EGF (Gibco) or PBS for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: Recombinant PfbA (rPfbA) or BSA was coated onto 0.5-µm-diameter fluorescent beads (FluoroSphere, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 ng of poly(dI-dC) were used for 100 ng of recombinant ERα (ThermoFisher, RP-310) and 1 µg for 10 µg of nuclear extracts ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit IgG antibodies (1:500 for IF) and recombinant human BMP2 (#PHC7145) were from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... and 10 μg/mL Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 555 Conjugate (CTSB, Fisher Scientific) to label GM1 as the acceptor ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel droplets were overlaid with recombinant organoid medium: Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were prepared in Spodoptera frugiperda (Sf9) cells using the Bac-to-Bac system (Life Technologies). Recombinant protein complexes were expressed in Spodoptera frugiperda by co-infection of single baculoviruses ...
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bioRxiv - Neuroscience 2021Quote: ... was added to each sample (4μl 5X First-Strand Buffer, 1μl 0.1 M DTT, 1μl RNaseOUT Recombinant RNase Inhibitor (Invitrogen; 10777019), 1μl of SuperScript III RT (200 units/μl) ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... fibroblast outgrowths were dissociated and split 1:4 using a recombinant trypsin-like enzyme (TrypLE Select, Invitrogen). Additional fibroblast preparations were commercially available ...
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bioRxiv - Immunology 2021Quote: ... The recombinant Fab was purified from culture supernatant using an anti-CH1 CaptureSelect column (Thermo Fisher Scientific). For the RBD/COV2-2196 complex ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant Rep1ΔAD-His and its variants were incubated with 50 μl D–Galactose agarose beads (Thermo Scientific) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
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bioRxiv - Molecular Biology 2022Quote: ... coli expression plasmids for purification of recombinant Cdc14 enzymes were generated using the Gateway cloning system (Invitrogen). S ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cardiomyocytes were labeled with of DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17, Invitrogen, MA1-142-A488) at 1:500 dilution in 1% BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant adenoviruses expressing the GRK2 shRNA were produced using BLOCK-iT™ Adenoviral RNAi Expression System (Invitrogen) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... The Dicer/TRBP enzyme was the mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were subsequently incubated by primary antibody 1:500 (GFP recombinant rabbit monoclonal antibody; G10362, Life Technologies) for 48 hours at 4°C followed by three PBS washes ...
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bioRxiv - Microbiology 2022Quote: ... and 50 seconds elongation at 72°C with 1U of Pfu recombinant DNA polymerase (Thermo Fisher Scientific). The amplicon ORF45HA was cut with NheI/SmaI and inserted in pEGFP-C1 (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... recombinant baculoviruses were generated in Sf9 cells using the Bac-to-Bac baculovirus expression system (Invitrogen, USA). HEK293F cells were cultured under 5% CO2 at 37 °C and were used for transfection at a density of 2.5 × 106 cells/ml ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were performed by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) and carried out in a total volume of 25 µL containing the following ...
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bioRxiv - Microbiology 2023Quote: ... 15μL of annealed guide RNA oligos were mixed with 15μL of 1μM recombinant Cas9 (IDT) in 220μL of Opti-MEM media (Gibco) and incubated for 5min at room temperature ...
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bioRxiv - Microbiology 2024Quote: Primary amines of recombinant EtpA were biotinylated with EZ-Link Sulfo-NHS-LC-LC-Biotin (ThermoFisher 21388) for 30 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Enzymes used for recombinant DNA cloning and Golden Gate Assembly were purchased from Thermo Scientific (Waltham, MA) and New England Biolabs (Ipswich ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... either the recombinant CT584 or purified Cm EB’S were solubilized in SDS sample buffer (Novex, Life Technologies) in the presence of reducing agent (10mM DTT) ...