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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Recombinant Human ISG15 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Recombinant GCase N370S mutant was produced by using 293T Freestyle cells (Thermo Fisher Cat. No. R79007) grown in suspension in 293 Freestyle medium (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Dicer/TRBP enzyme was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... beads were incubated in kinase reaction buffer supplemented with 100 ng recombinant CAMK2D (Invitrogen, cat. PV3373), 100 µM recombinant calmodulin (Enzo ...
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Immunology 2022Quote: ... For Cytokine Multiplex of Human Inflammation Panel (Invitrogen™ Inflammation 20-Plex Human ProcartaPlex™ Panel) (Catalog # EXP20012185901, eBioscience/Invitrogen) procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from human NSCLC cells and human clinical NSCLC tumor and normal samples by homogenization in Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... supernatants were diluted 4-fold and assayed with the Amyloid beta 40 Human ELISA Kit and either the Amyloid beta 42 Human ELISA Kit or the Amyloid beta 42 Human ELISA Kit Ultrasensitive (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Microbiology 2022Quote: ... encoding full-length human MCT1 (uniprot ID: P53985) and human basigin (uniprot ID: P35613-2) were cloned individually into pFastBac vector (Invitrogen) for recombinant expression in Sf9 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: Human colorectal adenocarcinoma (ATCC, #SK809) and human pancreatic carcinoma (ATCC, #CRL1469) cells were grown in McCoy’s 5A medium (Gibco, #16600082) and Dulbecco’s Modified Eagle’s medium (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Equivalent amounts of protein were resolved on precast polyacrylamide denaturing gels and were then transferred onto 0.2 μm PVDF membranes using either dry transfer with an iBLOT2 system (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured (Pierce BCA Protein Assay, Thermo Fisher Scientific) and a total of 100 µg of protein per sample was subjected for trypsin digestion ...
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bioRxiv - Cancer Biology 2020Quote: ... and protein was quantified using the Bradford protein assay (Thermo Scientific). For blotting ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was determined using the Qubit Protein Assay Kit (ThermoFisher). Oligonucleotide probes (Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein content was assessed using BCA Protein Assay Kit (Thermo Fisher). Equivalents of 20 μg of protein were taken for mass spectrometry (MS ...
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bioRxiv - Genetics 2021Quote: ... Protein concentrations were estimated with bicinchoninic acid protein assay (ThermoFisher Scientific). Ten mgs of each lysate were combined ...
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bioRxiv - Genetics 2019Quote: ... DNA-protein complexes were recovered with protein G magnetic beads (Invitrogen). Library preparation was performed as previously described34 ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein concentrations were measured with Pierce 660nm Protein Assay (Thermo Scientific), and 10 μg of protein were loaded on 4–20% FastGene SDS-PAGE gradient gels (Nippon Genetics) ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A or protein G Dynabeads (Thermo Fisher Scientific, 10001D, 10003D) was used for rabbit or mouse antibodies respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by the BCA protein assay kit (ThermoFisher). For storage ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was determined by bicinchoninic (BCA) protein assay (ThermoFisher Scientific) and samples were resolved on 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were quantified using the BCA protein assay kit (ThermoFisher Scientific). Total protein extracts were resuspended in 5X Laemmli buffer at a final concentration of 500 µg/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was assessed by Pierce BCA protein assay (Thermo scientific). For Western Blotting ...
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bioRxiv - Genomics 2020Quote: ... Dynabeads™ Protein A or Protein G (Invitrogen 10002D and 10003D) were washed 5 times with 5% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined by the BCA Protein Assay (Thermo Fisher). Whole cell lysates were resolved by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Bioengineering 2020Quote: ... Protein concentration was determined by BCA Protein Assay kit (ThermoFisher Scientific). Fifteen μ g of protein were resolved by electrophoresis on a 10% acrylamide gel and transferred to nitrocellulose membrane by standard methods ...
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bioRxiv - Neuroscience 2020Quote: ... and protein concentrationwas determined by MicroBCA Protein Assay kit (Thermo Scientific) according to the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was quantified with Pierce BCA protein assay kit (Thermo Fisher) and analysed with a Sunrise Tecan plate reader ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were determined using the microBCA protein kit (Thermo Scientific). Ten to 20 µg total cell or brain lysate ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were determined using the microBCA protein kit (Thermo Scientific). Ten μg synaptosomes were prepared in Laemmli buffer for Western blot analysis and 30 μg of synaptosomes were used for the mitochondrial potential assay.
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was quantified by BCA protein estimation (Thermo Scientific, #23225). 30 μg of sample protein and loading buffer (BioRad ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentrations were determined using the BCA protein assay kit (ThermoFisher). Equivalent amounts of protein were loaded and separated by 4-12% gradient SDS-PAGE and subsequently transferred to an Immobilon-P PVDF transfer membrane (EMD Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was measured by BCA protein assay kit (Thermo Fisher). One hundred μg of protein from each sample were digested with trypsin (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was estimated by BCA protein assay (Thermo Fisher Scientific), and 15 μg of total protein were supplemented with 2× SDS sample buffer (100 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... protein concentration was determined by Bradford protein assay (Thermo Fisher Scientific). Protein lysates were run on 7.5/10/15% SDS-PAGE (25-50 µg/lane ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was measured by Bradford protein assay (Thermo Fisher Scientific) and ~300 μg of reduced and alkylated proteins was subjected to trypsin digestion following the S-Trap manufacturer’s procedure ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was measured by Pierce BCA protein assay (Thermo Scientific). Protein samples were mixed with 4× Laemmli buffer (Bio-Rad) ...