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Citations for Thermo Fisher :
1501 - 1550 of 6825 citations for Recombinant Human GCK None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Flp-In T-Rex-293 cells were co-transfected with the plasmid coding for the tagged LTP and the pOG44 plasmid encoding the Flp recombinase (Invitrogen). Positive clones were selected by adding 100 µg/ml hygromicin B and 15 µg/ml blasticidin on the day after transfection ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: Rescue experiments were conducted in LRBA-KO HEK293T and HaCat cell lines transfected with 2 μg of WT LRBA plasmid tagged with myc (Origen) using Lipofectamine 2000 (Invitrogen). These reconstituted cells lines are called Myc-LRBA in this manuscript ...
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bioRxiv - Molecular Biology 2024Quote: ... the mixture of 1.0 μg GFP-tagged TDP-43 CTFs and 1.0 μg RFP-tagged TDP-43 for two color co-expression) were transfected using Lipofectamine 2000 (Thermo Fisher). After 1 overnight incubation for transfection ...
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bioRxiv - Biochemistry 2024Quote: BRSK1 and 2 were cloned into pDest vectors (to express N-terminal Flag or HA tagged proteins) using the Gateway LR Clonase II system (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Live microscopy of parasites expressing GFP-tagged proteins was performed by staining mitochondria with 25 nM MitotrackerRed CMXRos (Invitrogen, M7512) for 30 minutes and staining nuclei with 5 µg/mL Hoechst 33342 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Randomisation was removed and samples were mass tagged using 10 channels of a TMT-11-plex stock (Thermo Fisher Scientific). For quantification control ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids for expression of SUMO-tagged efpL genes were generated with the Champion™ pET-SUMO Expression System from Invitrogen™ according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... then cloned into the destination vector pEarleyGate101 with YFP-tagged sequences under the control of Pro35S using the LR recombination kit (Invitrogen) to obtain 35S::NPR1-YFP and 35S::TGA3-YFP constructs ...
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bioRxiv - Neuroscience 2020Quote: Levels of total APOE protein were analysed by Human ELISA Kit from Invitrogen (Apolipoprotein E Human ELISA Kit). Levels of Aβ were analysed by ELISA (Human β Amyloid 42 ELISA Kit Wako high sensitive #298-64401 and Human β Amyloid 40 ELISA Kit Wako #298-64601 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for human cells are mouse anti-human CD3 conjugated to APC (Thermo Fisher, 47-0036-42), mouse anti-human CD8 conjugated to FITC (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... where EGF and noggin were replaced with human forms with 10 ng ml−1 human EGF (Invitrogen, PHG0311), 100 ng ml−1 human noggin (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... which contains the gene expression profile of human microglia using a human gene ST 2.0 Microarray Chip (Affymetrix). The human microglia were polarized to either pro-inflammatory (M1 ...
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Bioengineering 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Immunology 2023Quote: Total human IgG levels were determined following the manufacturer’s instructions in the Human IgG Total ELISA Kit (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cells were co-stained with recombinant Cholera toxin subunit B conjugated to Fluor488 (Invitrogen C34775) at 1 μg/mL for less than 1 minute prior to fixing and staining for NINJ1 as described above ...
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bioRxiv - Physiology 2019Quote: Recombinant adenoviruses were generated using the pAd/CMV/V5-DEST Gateway recombination system (Life Technologies) after cloning the full-length cDNA into the pENTR vector ...
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bioRxiv - Biochemistry 2019Quote: ... The resulting antiserum was affinity purified using recombinant CTLMA2 immobilized on Aminolink resin (Thermo Fisher). Western blotting was performed with affinity purified anti-CTLMA2 ...
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bioRxiv - Immunology 2019Quote: ... The protein was expressed in Sf9 insect cells using a recombinant baculovirus system (Life Technologies). The protein was purified on a cobalt resin column and further purified by gel filtration to ensure the removal of monomeric and aggregated protein.
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bioRxiv - Biochemistry 2021Quote: ... Recombinant BACs were isolated from the bacterial hosts and used to transfect Sf9 cells (Invitrogen).
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bioRxiv - Biochemistry 2021Quote: ... The recombinant IgG (XBA14 and XBA18) was produced in FreeStyle 293 cells (Thermo Fisher Scientific), and purified by Protein A-Sepharose (Cytiva ...
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bioRxiv - Bioengineering 2019Quote: ... and genomic DNA was digested using Optizyme™ recombinant DNase-I digestion kit (Thermo Fisher) per manufacturers’ instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant PfAdoMetDC protein was purified using the Strep-Tactin Sepharose column (Invitrogen, California, USA) as described previously (29 ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of recombinant IL-18 was induced by 0.5 mM IPTG (Thermo-Fisher Scientific) for 8 h at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with recombinant EGF and bovine pituitary extract according to the manufacturer’s instructions (Thermo Fisher), as well as 100 μg/ml Primocin (Invivogen) ...
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bioRxiv - Microbiology 2020Quote: ... BMDCs were differentiated with recombinant murine granulocyte-macrophage colony-stimulating factor (20 ng/ml; Invitrogen), while BMDMs with macrophage colony-stimulating factor (10ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) and the purity was assessed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Biophysics 2021Quote: ... and 6xHis tags Recombinant baculoviruses were generated using the Bac-to-Bac system (Thermo Fisher). High Five cells (Thermo Fisher B85502 ...
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bioRxiv - Microbiology 2019Quote: ... Samples were shifted to RT and treated with 1 µl RNaseOUT Recombinant Ribonuclease Inhibitor (ThermoFisher) and 0.5 µl Thermo Scientific Maxima H Minus Reverse Transcriptase ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Plant Biology 2021Quote: ... Gateway recombinant cloning was performed to subclone the aforementioned constructs into pDEST™24 (Invitrogen) using a LR cloning reaction (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant plasmids were isolated by using Gene JET plasmid miniprep kit (Thermo Scientific, USA). The recombinant plasmids of SCD and SREBP1 genes were transfected into the CEH by using the Gene Pulser XcellTM Electroporation system (Biorad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant baculoviruses expressing A2AAR WT or mutants were prepared using Bac-to-Bac system (Invitrogen). Spodoptera frugiperda 9 (Sf9 ...
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bioRxiv - Cell Biology 2021Quote: Binding of recombinant proteins to pre-spotted PIP and membrane lipid strips (Thermo Fisher; P23751) was performed according to (Dowler et al. ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ng Cholera Toxin subunit B (CT-B) (Recombinant Alexa Fluor 488 conjugate, Life Technologies) was injected into the hindbrain ventricle81 ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant lentivirus was produced by transient transfection of 293T cells using Virapower packaging mix (Invitrogen). Cells were plated into 2 well LabTek chambered coverglass (Nunc ...
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bioRxiv - Cell Biology 2019Quote: ... Transposition and the generation of recombinant baculovirus were performed following manufacturer’s protocols (Thermo Fisher Scientific). For protein production ...
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bioRxiv - Immunology 2020Quote: ... 100uL of recombinant protein at 0.5 ug/ml was coated on Maxisorp™ plates (Nunc) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CP1Ag was expressed and initially purified via a nickel-affinity column (HisPur Thermo Fisher) as previously described [64] ...