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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Caspase 7 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were quantified with Pierce™ BCA Protein Assay Kit (Life Technologies) and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant T-REx-HEK-CFTR cells were generated by lipofectamine transfection (Life Technologies) of plasmids containing mutated versions of CFTR (RefSeq NM_000492) ...
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bioRxiv - Biochemistry 2023Quote: ... 6 µg RNA was used and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant chimeric antibodies were produced using the Expi293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated recombinant toxins were captured using streptavidin-coated Dynabeads (Invitrogen, M-280). After washing with PBS containing 0.1% (v/v ...
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bioRxiv - Physiology 2023Quote: ... Recombinant TEVp was purified as previously described1 or was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... were coated with 50 ng of recombinant Gag/well (35) in 1xPBS (Gibco) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The recombinant NS1 gene was cloned into the pTRC–His2c expression vector (Invitrogen) and expressed in the BirA-enzyme containing E ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and incubated with recombinant enzymes TrypLE (Thermo Fisher) for 10 minutes at 37°C for single cell dissociation ...
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bioRxiv - Neuroscience 2024Quote: For recombinant lentivirus production - HEK293FT cell line (cat# R70007, ThermoFisher Scientific, Auckland, NZ), HT1080 cell line (cat# CCL-121 ...
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Immunology 2019Quote: DNA plasmids (Caspase-1-GFP and NOD1-mOrange) 59 or specific silencer and negative control siRNA (40 μmol of each; ThermoFisher Scientific) were diluted in Opti-MEM medium (Gibco ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic) using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then loaded at room temperature for 30 min with fluorescent indicator-linked substrates for activated caspase-8 (10 µM Z-IETD-R110; Molecular Probes), caspase-9 (10 µM ZLEHD-R110 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then loaded at room temperature for 30 min with fluorescent indicator-linked substrates for activated caspase-8 (10 μM Z-IETD-R110; Molecular Probes), caspase-9 (10 μM Z-LEHD-R110 ...
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bioRxiv - Cell Biology 2021Quote: ... the Smac-mimetic compound A (500 nM) and the pan-caspase inhibitor IDN-6556 (10 μM) (TSI) to induce necroptosis in FluoroBrite DMEM media (ThermoFisher Scientific) supplemented with 1% FCS ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were lysed and processed for Caspase-3 activity assay by following the manufacturer’s instructions (#E-13183, Thermo Fisher Scientific). At the end of the assay ...
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bioRxiv - Biochemistry 2021Quote: ... Primary Strep-MAb classic chromeo-555 (IBA) and secondary Alexa-fluor-488 or −555 goat anti-mouse (Invitrogen) were applied sequentially with PBS washes in between ...
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bioRxiv - Bioengineering 2021Quote: ... cells were pre-incubated with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 µL of PBS with 2% FCS for 15 minutes at 4 °C ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Briefly, sheep-anti mouse IgG paramagnetic beads (Dynal, 11201D) were coupled with anti-His mAb (Invitrogen, 37-2900) in coating buffer (PBS plus 0.1% immunoglobulin-free BSA ...
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bioRxiv - Immunology 2021Quote: ... or with unconjugated 4.3.1.3 mouse MAb and secondary detection with goat anti-mouse Alexa-Fluor 647 (A-21235; Life Technologies). Immunofluorescence was used to boost GFP signal in TgBAC(lck:gfp)vcc6 specimens with a chicken anti-GFP primary (Abcam ...
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bioRxiv - Biophysics 2022Quote: ... phagosomes were washed 3X with MAB and incubated with secondary antibodies against mouse (Alexa488; A11029, Thermo Fisher Scientific) and rabbit (Alexa647 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The bound mAb was detected with adding 100 μl/well of 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Life Technologies) and incubation at room temperature for 5 min prior to the addition of 100 μl of 3% H2SO4 to stop the reaction ...
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bioRxiv - Microbiology 2021Quote: ... The isotyping of the selected clones was performed using the commercial Rapid ELISA Mouse mAb Isotyping kit (ThermoFisher). The kit determines the presence of the murine isotypes IgG1 ...
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bioRxiv - Immunology 2020Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... we obtained the sequence information of the 14.4.4 mAb VH and VL chain by isolating total mRNA from a 14.4.4 mAb-producing hybridoma cell line with the use of TRIzol (Invitrogen) followed by reversely transcribing the isolated mRNA into cDNA (First-strand cDNA Kit ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated with mouse anti-human E-cad ectodomain mAb (1:5000) (HECD-131700, Invitrogen, USA) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 1 h in anti-HA 2-2.2.14 IgG1 mouse mAb (ThermoFisher Catalog# 26183; RRID:AB_10978021) diluted 1:2000 and following 3 x 10 min washes in 0.1 M PB ...
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bioRxiv - Immunology 2023Quote: ... mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific) followed by removal of unreacted biotinylation reagents using a Zeba Spin Desalting Column (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... double immunofluorescence labeling with detergent was performed on 50-µm sections with the anti-pTau mAb AT8 (ThermoFisher) plus the anti-mouse IgG H + L secondary antibody conjugated with Alexa Fluor 488 (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD8+ T cells were stimulated with αCD3 and αCD28 mAb-coated dynabeads (Thermo Fisher Scientific, no. 11452D).
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bioRxiv - Microbiology 2024Quote: ... mAb pool was dialyzed in PBS pH 7.4 using Slide-A-Lyzer G2 Dialysis Cassette 3.5K (Thermo Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GFP rabbit (A11122, Invitrogen, CA, USA), chicken anti-GFP (AB16901 ...
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bioRxiv - Cancer Biology 2021Quote: ... in a ViiA 7 or 7900 HT Real-Time PCR system (Applied Biosystems). Data analysis was performed using the RQ Manager (Applied Biosystem ...
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bioRxiv - Molecular Biology 2020Quote: ... MCF-7 passage cell line were prepared using 0.05% trypsin solution (Invitrogen, Carlsbad) and seeded in 96 well tissue culture plates ...
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bioRxiv - Cell Biology 2020Quote: ... purified CD14+ monocytes were differentiated for 7 days in RPMI-1640 medium (GIBCO) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... U-2OS and COS-7 was carried out with Lipofectamine 3000 (Life Technologies), Fugene6 (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was carried out on a ViiA 7 thermocycler (Thermo Fisher Scientific) and using standard protocol as provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... 7-10 µg of RNA was denatured in 2x RNA load dye (Thermofisher) and heated to 65°C for 15 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Organoids were passaged every 7-10 days by disruption with TrypLE (Thermo Scientific) and reseeded at a concentration of 100-500 organoids per well.
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bioRxiv - Genomics 2020Quote: ... or a positive control probe 5’-5Alexa488N/(ATA)8TUU (ATA)7-3’ (Invitrogen). Reactions were incubated in a water bath at 37°C for 2 hrs ...
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bioRxiv - Immunology 2022Quote: ... The reactions were carried out on a QuantStudio 7 Flex System (Applied Biosystems) according to the manufacturer’s instruction under the following reaction conditions ...