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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized using isofluorane and perfused transcardially with 4 mL PBS followed by 4 mL RNAlater™ (Invitrogen; CAT # AM7020) using a peristaltic pump at a flow rate of 0.8mL/min ...
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bioRxiv - Biophysics 2021Quote: ... The grids were blotted at blotting force 20 for 4 s at 4°C and 100% humidity and plunged into liquid ethane using a Vitrobot (Thermo Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... cell suspensions obtained as above were fixed in 4% paraformaldehyde and cytocentrifuged (Cytospin 4 Shandon, Thermo Scientific, 1200 rpm, 10 minutes). Slides of captured cells (n=3 patients for each group ...
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bioRxiv - Immunology 2019Quote: ... 100 μg/mL streptomycin with either 110 U/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) (Leukine; Sanofi) and 282 U/mL interleukin-4 (IL-4) (Affymetrix, eBioscience) for DCs or 280 U/mL GM-CSF for macrophages ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cellular response in each well was measured via fluo-4 intracellular calcium dye using the fluo-4 NW calcium assay kit (Molecular Probes) according to manufacturer’s directions ...
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bioRxiv - Bioengineering 2019Quote: ... and CarboPac PA1 standard bore guard column (4 x 50 mm) plus CarboPac PA1 preparative IC column (4 x 250 mm, both Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2022Quote: Protein samples in 1X LDS sample buffer containing 4% β-mercaptoethanol (BME) were separated by size on 4-12% Novex NuPage Bis-Tris SPS-PAGE (Invitrogen) gels (2 h ...
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bioRxiv - Genetics 2022Quote: ... Larval brains were fixed in 25 ml of acetic acid: 4% formaldehyde in PBS (45%:55%) for 4 min on a clean Superfrost plus slide (Fisher Scientific) and the sample was manually squashed via thumb/stamp over coverslip ...
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bioRxiv - Plant Biology 2022Quote: ... from cauline leaves of flowering plants by measuring the quantity of 4-methylumbelliferone product generated from the substrate 4-methylumbelliferyl-b-D-glucuronide (Duchefa) on a fluorometer (Fluoroscan II; Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, NW04122BOX) and transferred onto a PVDF membrane (Immobilon-FL ...
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bioRxiv - Neuroscience 2022Quote: ... Three successive 5-hour paraffin wax embedding stages were performed followed by cooling and sectioning of tissue on a Leica microtome into 4 × 4 μm thick serial sections that were collected on Superfrost (ThermoFisher Scientific) microscope slides ...
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bioRxiv - Physiology 2024Quote: ... only 4 wells of cells were incubated in DMEM with 4 μM fura-2/AM (fura-2/AM, Molecular Probes, USA) at 37° C in the dark for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Separation of anionic metabolites were performed using a Dionex IonPac AS11-HC-4 µm column (250 × 0.4 mm, 4 µm; Thermo Fisher Scientific). The injection volume was 0.4 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... The mixture of SUMO protease cleavage products was dialyzed overnight (4 ºC, 4 L, 20 mM Tris-HCl, 150 mM NaCl, Slide-A-Lyzer→ cassette, 10 kDa, Thermo Scientific). The hexa-histidine tagged SUMO protein was separated from non-tagged authentic Mpro present in the dialysate by IMAC and collecting Mpro in the flow through ...
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bioRxiv - Cell Biology 2024Quote: ... Click-iT reaction cocktail (100 Mm Tris-buffered saline, 4 mM CuSO4, 100 mM ascorbic acid and 4 µM Alexa FluorTM488 azide (Invitrogen #A10266) was used for detecting EU ...
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bioRxiv - Neuroscience 2024Quote: ... larvae and dissected adult brains were fixed overnight in paraformaldehyde (PFA; 4% in 1x phosphate-buffered saline) at 4°C then dehydrated overnight in 100% methanol (A4124, Fisher Scientific) at -20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... (4) 30 min incubation in ammonium chloride (NH4Cl) and (5) 4 min incubation in Tissue Autofluorescence Quenching Kit (ReadyProbes, ThermoFisher Scientific).Secondary antibodies were used as follows ...
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bioRxiv - Immunology 2023Quote: ... Invitrogen E-Gel EX Agarose Gels 4%, and DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, D1306) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... -4 blot force for 4 seconds and plunged frozen in liquid ethane using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected on a Titan Krios operating at 300 keV and equipped with a Gatan K3 direct detector and a 20 eV slit width energy filter ...
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bioRxiv - Plant Biology 2023Quote: ... Denatured samples were spun down at 5,000 xg for 3 minutes and supernatant was run on 4%-20% Bio-Rad 4%-20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... MBL) for 4 days at 4°C and with Alexa-Fluor (AF) 488 goat anti-rabbit IgG (R37116, Thermo Fisher Scientific) overnight at RT followed by DAPI staining (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 22,000 × g at 4°C for 20 minutes and poured over 4 mL of HisPur Cobolt resin (89964, Thermo Fisher Scientific). The slurry was rotated at 4°C for 1 hour and spun down at 700 × g for 2 minutes ...
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bioRxiv - Genetics 2023Quote: ... Extracts were incubated with rotation with rabbit polyclonal anti-Piwi antibodies (4 µg per sample) at 4°C for 4h followed by overnight incubation with DynabeadsTM Protein A (50 µl, Invitrogen, 10002D) at 4°C with rotation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: A fraction of the liver from each mouse was preserved in 4% formalin (Cat# SF100-4, Thermo Fisher Scientific, Waltham, MA) followed by 70% ethanol for histology ...
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bioRxiv - Immunology 2023Quote: ... blotted for 4 s in 100% humidity at 4 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher). All grids were screened using a ThermoFisher Glacios microscope (OSU Center for Electron Microscopy and Analysis).
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bioRxiv - Cell Biology 2023Quote: ... Extracts were clarified by centrifugation twice for 15 minutes at 16,000 rcf at 4°C and 100 μl embryo extract was rotated at 4°C overnight with 30 μl magnetic ProteinA dynabeads (Life Technologies) coupled with anti-MBP antibodies (gift from Jordan Raff ...
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were washed with ice-cold PBS, harvested, and incubated (30 min, 4 °C) with rabbit anti-AF488 IgG (4 µg/mL, Thermo Fisher). AF488 fluorescence was quantified by flow cytometry (BD FACSCanto II flow cytometer ...
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bioRxiv - Cancer Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 mm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive ...
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bioRxiv - Plant Biology 2023Quote: ... Denatured samples were spun down at 5,000 g for 3 min and supernatant was run on 4–20% Bio-Rad 4–20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Systems Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 μm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive high-performance benchtop quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Denatured samples were spun down at 5,000 xg for 3 minutes and supernatant was run on 4%-20% Bio-Rad 4%-20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody or IgG (as control) was added and incubated for 4 hrs at 4°C with Protein G Dynabeads (Life Technologies) for antigen coupling ...
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bioRxiv - Microbiology 2020Quote: ... CHIKV was labeled with the lipophilic fluorescent probe DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt; Life Technologies), as described previously [5] ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE was performed using NuPAGE 4-12% Bis-Tris and 3-8% Tris-Acetate gels (Life Technologies). Nitrocellulose membranes were developed with horseradish peroxidase (HRP ...
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bioRxiv - Genomics 2019Quote: ... Primary antibodies (anti-human CD47-APC; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3; and clone B6H12.2, ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: mRNA was collected from groups of 10 whole larvae (n=3–4 replicates per line) using Trizol (ThermoFisher) and reverse transcribed to cDNA using SuperScript III Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... and Antibiotic-Antimycotic Mixed Stock Solution (Nacalai) and were passaged every 3-4 days with TryPLE (ThermoFisher Scientific). For microscopy observations ...
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bioRxiv - Molecular Biology 2020Quote: ... a sample of eluates (3%) was separated on acrylamide NuPAGE Novex 4–12% Bis-Tris gels (Life Technologies) and analyzed by silver staining.
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bioRxiv - Molecular Biology 2019Quote: ... Cells were passaged every 3-4 days with 0.25% trypsin-EDTA solution (Life Technologies, cat. no. 25200-056) and washed with sterile PBS (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-4 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Biochemistry 2022Quote: ... and then analysed on Novex WedgeWell 4-12 % Tris-Glycine Gels or 3-8 % Tris-Acetate gels (Invitrogen). Tris-Glycine gels were run at 180 V for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... or X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside, W5376C; Thermo Fisher Scientific, Guilford, CT). GUS and/or LacZ-stained tissues were cleared for 30 s with 12 % sodium hypochlorite solution before microscopy observations.
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bioRxiv - Cell Biology 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000015). Viral supernatant was harvested 48h after transfection and filtered through 0.45 µm cellulose acetate filters (Corning 431220) ...
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bioRxiv - Cancer Biology 2024Quote: ... at 95⁰C for 3 min and loaded onto a SDS-polyacrylamide gel (4-12%, Invitrogen NuPAGE, #NP0336BOX). After size separation ...
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bioRxiv - Genomics 2023Quote: ... All nuclei were pooled and stained with DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306). Using a FACSAria III cell sorter (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (Thermo Fisher Scientific, cat.: 34042) was added and the reaction was terminated after 5 min under running tap water ...
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bioRxiv - Biochemistry 2023Quote: ... the filtered supernatant was loaded onto 3-4 mL immobilized D-galactose gel (Pierce™, Thermo Scientific™) equilibrated by gravity flow with Gal A buffer (50 mM Na-phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were marked with 2,3x10-3 µg/µL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 min at RT in the dark ...
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bioRxiv - Genomics 2024Quote: ... The remaining cells are resuspended in our working cell buffer solution (AMES/0.4% BSA + 3 µM ActD) and incubated with CD90.2/Thy1.2 antibody conjugated to APC (#17-0902-83; Invitrogen, Carlsbad ...