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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for 6' ETHYL SPIRO 1 3 DIOXANE 2 3' INDOLIN 2' ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated at 4°C for 3 hours on a rotator before being placed in a DynaMag™-2 magnetic rack (Thermo Scientific, 12321D) and washed twice with TBS+NP40 wash buffer ...
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bioRxiv - Molecular Biology 2019Quote: The 2′ O-methyl phosphorothioate AOs were transfected into dermal fibroblasts as lipoplexes using 3 μl of Lipofectamine 3000 (Life Technologies, Melbourne, Australia) per 1 ml of OptiMEM ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 3 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL min−1 for 3 min ...
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E-cadherin regulates the stability and transcriptional activity of β-catenin in embryonic stem cellsbioRxiv - Cell Biology 2021Quote: ... Cells were replated at a density of 2×103 cells/cm2 every 3 days after dissociation with 0.25% trypsin–EDTA (1X) (Gibco, cat no. 25200-056). Cells were grown in Knockout DMEM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... An adenylated adaptor was ligated to the 3’-end of the captured transcripts (Supplementary Table 3) by using a mixture of T4 RNA ligase and truncated T4 RNA ligase 2 (ThermoFisher Scientific and NEB), which served as a template for the reverse transcription primer (Supplementary Table 3) ...
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bioRxiv - Genetics 2021Quote: ... Five microliters of peptide mixture were loaded onto an Acclaim PepMap™ precolumn (75 μm x 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm x 50 cm ...
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bioRxiv - Cell Biology 2022Quote: ... The growth medium was changed every 2 – 3 days and consisted of RPMI-1640 (for the RIN14B cells) (Fisher Scientific, 11-875-093) or otherwise DMEM/F12 (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were first trapped for 3 min on a C18-reverse phase trapping column (Acclaim PepMap® 100, 75 μm x 2 cm, particle size 3 μM, nanoViper, Thermo Fisher Scientific), followed by separation on a C18-reverse phase analytical column (Acclaim PepMap® 100 ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were pre-concentrated and desalted online using a PepMap C18 nano-trap column (length - 2 cm; inner diameter - 75 μm; particle size - 3 μm; pore size - 100 Å; Thermo Fisher Scientific) with a flow rate of 3 μL/min for 5 min ...
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bioRxiv - Immunology 2023Quote: ... The larvae were washed 2 additional times with 40 mls of sterile water followed by 3 washes with 40 mls of sterile PBS (Gibco™ PBS [10X]). Larvae were counted under a dissecting microscope using a McMaster slide and stored in vented U-shaped 275 ml tissue culture flasks (Polystyrene tissue culture flasks with vented caps ...
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bioRxiv - Molecular Biology 2022Quote: ... TFA as mobile phase on a trap column (Acclaim PepMap100, 2 cm length, 3 μm C18 particle size, 100 Å pore size, 75 μm inner diameter, Thermo Fisher Scientific) and finally separated at 60 °C using a 2 h gradient from 4 to 40 % (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... The plates were blocked by 3% BSA/PBS for 2 h and then incubated with serial dilutions of an EPO standard (Thermo Fisher Scientific, USA)) or culture supernatant samples for 4 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from the LV myocardium of 2-month-old male WT and Rbm20ΔRS mice (n = 3 per group) using TRIzol Reagent (Life Technologies, Waltham, MA, USA) and treated with DNase I (cat# D9905K ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated and washed for 3 min on a C18 reverse phase precolumn (3 μm particle size, 100 Å pore size, 75 μm inner diameter, 2 cm length, from Thermo Fisher Scientific). Peptides were separated on an Aurora C18 reverse phase resin (1.6 μm particle size ...
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bioRxiv - Neuroscience 2023Quote: ... A full media change was performed every 2-3 days and cells were split by dissociation with TrypLE Express Enzyme (Gibco, cat no: 12605010).
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bioRxiv - Cell Biology 2023Quote: Aliquots of each sample were loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 % SDS) for 30 minutes at 50 °C prior to reprobing using mouse anti-3-phosphoglycerate kinase anti-serum (Invitrogen-Molecular probes, A6457).
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bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... mixed with forward and reverse detection primers (T3DCD S1 forward [5’- TACGCGTTGATCACGACAAT-3’] and T3DCD S1 reverse [5’- TGGCGAGATTATTCCCTGAC-3’] or GAPDH forward [5’- ACCCAGAAGACTGTGGATGG-3’] and GAPDH reverse [5’- GGATGCAGGGATGATGTTCT-3’]) and SYBR Select Master Mix (Applied Biosystems), and then subjected to PCR using the StepOnePlus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... and customized si-KIF4 3′ UTR targeting endogenous KIF4 mRNA 3’-UTR region (5′-GGAAUGAGGUUGUGAUCUUTT-3′) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... medium (1:1) supplemented with N-2 (Gibco), B-27 (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... one half of the Dicer-2 beads were incubated with 100 U RNase I (Ambion) in 100 µl IPP 150 at 25°C for 15 min on a thermomixer ...
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bioRxiv - Plant Biology 2022Quote: ... and 2 mL of 1:10 diluted cDNA in an Applied Biosystems Step One Plus real-time PCR instrument (ThermoFisher, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 4T1 cells were injected and mice treated with either 1 or 3 doses of 8 mg/kg carboplatin or vehicle by tail vein injection staggered by one day with anti-VEGFR3 antibody (100 μg per injection × 3 total injections, I.P., eBioscience (now ThermoFisher), Control IgG ...
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bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative expression was calculated using the ΔΔCT method ...
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bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative genomes were calculated using the ΔCT method ...
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bioRxiv - Immunology 2024Quote: ... One cm pieces of small intestine were then incubated in HBSS containing 3 mM EDTA and 7.5% FBS (ThermoFisher) for 1 hour in a shaker at 37° C ...
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bioRxiv - Genomics 2023Quote: ... One microliter of each cDNA sample was used for TaqMan PCR (Eppendorf RealPlex Mastercycler and Applied Biosystems QuantStudio 3) with 50 heating cycles at 94°C for 30 seconds ...
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bioRxiv - Neuroscience 2023Quote: Primary mouse microglia were plated at a density of 3×105 in 6 well plates and treated for 6 h prior to RNA extraction with Trizol (Invitrogen; Taïb et al., 2013). Adult microglia were treated as described in section 2.2.3 prior to extraction of total RNA using RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... labeled peptides on one spin tip from a High-Select™ TiO2 Phosphopeptide Enrichment Kit (capacity of 1–3 mg; Thermo Fisher Scientific, catalog A32993). After preparing spin tips ...
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bioRxiv - Immunology 2022Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S ‘2P’ and HexaPro IMAC elution fractions were concentrated to ∼ 1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Immunology 2020Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S-2P IMAC elution fractions were concentrated to ~1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
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bioRxiv - Microbiology 2023Quote: ... cells were blocked for 1 h in 3% BSA/PBS and incubated with rabbit-anti-GFP (1:50 in 3%BSA/PBS, 200 µl/well, Invitrogen, G10362) for 1 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... viable CD45-CD34+Sca-I- cells were sorted from epidermal single cell suspensions of 1-month-old EGFRΔEgr2 (n=3) and littermate controls (n=3) into Trizol LS Reagent (Thermo Fisher Scientific) using a FACS Aria III ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Genetics 2024Quote: ... cell pellets were resuspended into PBS with 2% FBS and 1µg/ml of 4’,6-diamidino-2-phenylindol (DAPI) (Thermo Fisher Scientific, Cat#D1306) and transferred into 5 ml round bottom polystyrene flow tubes with cell strainer (Corning Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... 10% 3 kD or 10 kD biotinylated dextran amines (3 kD BDA, Invitrogen D7135 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with following primary antibodies and/or labelling agents: anti-GFP (Invitrogen, #11122, 1:800, 3 days or Invitrogen, #10262, 1:800, 3 days), anti-V5 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and pMD2G—at a 2:2:1 ratio using Lipofectamine 2000 (Thermo-Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... with 2 Units of S1 enzyme (ThermoFisher, 2 uL of a 1:100 dilution) and 1X S1 Buffer ...
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bioRxiv - Microbiology 2020Quote: ... COL1A1 (5’-CGAAGACATCCCACCAATC-3’ and 5’-ATCACGTCATCGCACAACA-3’), ALP (5’-TCACTCTCCGAGATGGTGGT-3’ and 5’-GTGCCCGTGGTCAATTCT-3’) (IDT, Coralville, USA) and viral non-structural (nsP1) gene (Applied Biosystems, USA) (5’-GGGCTATTCTCTAAACCGTTGGT-3’ and 5’-CTCCCGGCCTATTATCCCAAT-3’ ...
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bioRxiv - Clinical Trials 2019Quote: ... (Bruner and Siliciano, 2018), env (Forward: 5’-AGTGGTGCAGAGAGAAAAAAGAGC-3’, Reverse: 5’-GTCTGGCCTGTACCGTCAGC-3’, Probe: 5’/VIC/CCTTGGGTTCTTGGGA/3’/MGB) (Thermo Fisher Scientific) (Bruner et al. ...
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bioRxiv - Clinical Trials 2019Quote: ... (Palmer et al., 2003) and pol (Forward: 5’-GCACTTTAAATTTTCCCATTAGTCCTA-3’, Reverse: 5’-CAAATTTCTACTAATGCTTTTATTTTTTC-3’, Probe: 5’/NED/AAGCCAGGAATGGATGGCC/3’/MGB) (Thermo Fisher Scientific) (Schmid et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... or sgKLF5 pool (5’- GUGCGCUCGCGGUUCUCUCG-3’; 5’- AGGACGUUGGCGUUUACGUG-3’; 5’- GCGUCAAGUGUCAGUAGUCG-3’) was transfected per well using Lipofectamine™ RNAiMAX (Thermofisher, 13778150). Media was changed after 72 hours for longer treatments.
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bioRxiv - Immunology 2022Quote: ... custom primers and probes designed to amplify and label the Chlamydia 16S gene were used (forward: 5’-GGAGGCTGCAGTCGAGAATCT-3’; reverse: 5’-TTACAACCCTAGAGCCTTCATCACA-3’; probe 5’-6FAM-TCGTCAGACTTCCGTCCATTGCGA-TAM-3’; Fisher Scientific/Eurofins).