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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Saint Louis Encephalitis Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Midi Protein Gel (Invitrogen) for 45–60 min at 175 V ...
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bioRxiv - Plant Biology 2022Quote: ... Dynabeads Protein A (Invitrogen) was used to pull down polyclonal antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... DynabeadsTM Protein A (Invitrogen) in ChIP dilution buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Mini Protein Gels (Invitrogen). Electrophoresis occurred at 150 V ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein G Dynabeads (ThermoFisher) were first blocked with Block solution (0.5% BSA (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Mini Protein Gels (Invitrogen) using MES buffer ...
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bioRxiv - Cell Biology 2024Quote: Protein G Dynabeads (Invitrogen) were washed twice in 1ml 0.1M Na-phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G Dynabeads (Invitrogen) were conjugated with an α-FLAG antibody (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein G Dynabeads (Invitrogen) were washed 3 times in blocking solution (PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein G DynabeadsTM (Invitrogen) were blocked at 4°C overnight using 100 ng per 10μl of beads ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A Agarose (Invitrogen) and Filipin (SAE0087 from Sigma ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Dynabeads protein A (Invitrogen) and Dynabeads protein G (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Protein G-agarose (Invitrogen), and anti-IgM-agarose (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Dynabeads Protein G (Invitrogen) was washed with wash buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: ... Protein G Dynabead (ThermoFisher) was washed with PBS ...
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bioRxiv - Genetics 2023Quote: ... Protein A Dynabeads (Invitrogen) previously coupled with 10 μl of T7-Tag antibody per 1×107 cells in blocking solution (1x PBS ...
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bioRxiv - Immunology 2022Quote: ... Protein A Dynabeads (Invitrogen) were prepared according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... Protein G beads (ThermoFisher) were complexed with antibody and the antibody-bead complexes were incubated with cell lysates at 4C overnight with rotation ...
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bioRxiv - Genomics 2023Quote: ... Protein-G-Dynabeads (Invitrogen) were pre-coated with antibodies for 4 hours at 4°C and the following antibodies were used ...
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bioRxiv - Cell Biology 2023Quote: ... Mini Protein Gel (ThermoFisher). After SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Protein-G Dynabeads (Invitrogen) were washed 3x in PBS at 4°C.
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bioRxiv - Molecular Biology 2023Quote: ... Protein A Dynabeads (Invitrogen) were added for 1 hour to enrich fragments of interest ...
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bioRxiv - Genomics 2023Quote: ... Dynabeads Protein A (Invitrogen) were washed ...
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bioRxiv - Immunology 2023Quote: ... Midi Protein Gel (Invitrogen) for 45 - 60 min at 175 V ...
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bioRxiv - Plant Biology 2024Quote: ... Protein G Dynabeads (Invitrogen) coupled with anti-FLAG M2 antibody (F1804 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein A Dynabeads (Invitrogen) were equilibrated with wash buffer (NP-40 omitting lysis buffer) ...
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bioRxiv - Microbiology 2024Quote: ... Protein G dynabeads (Thermofisher) were coated with rabbit α-myc antibody (Cell Signalling Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... A similar protocol was followed for small scale virus production when establishing individual stable cell lines with transfection being performed on 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) reagent.
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bioRxiv - Microbiology 2021Quote: ... Each 10-μl RT-qPCR mixture consisted of 2.5 µL 4X TaqMan Fast Virus 1-Step Master Mix (Life Technologies, Carlsbad, CA, USA), 400 nM each primer ...
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bioRxiv - Cell Biology 2021Quote: ... the extracellular RSV virus were collected by PEG6000 precipitation method and intracellular RSV virus were collected by lysing the macrophage cells with the lysis buffer from mirVanaTM miRNA Isolation Kit (Ambion, Life Technologies, AM1560). Total RNA from RSV infected macrophage cells as well as stock virus was extracted using mirVanaTM miRNA Isolation Kit according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: The full-length cDNA for HA or a mutated gene and neuraminidase genes of influenza A/Switzerland/9715293/2013 (H3N2) virus were amplified by using SuperScript One-Step RT-PCR (Invitrogen, Grand Island, NY), and the 6:2 recombinant viruses with six internal genes of influenza A/PR/8/1934(H1N1 ...
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bioRxiv - Microbiology 2020Quote: The TaqMan real-time RT-PCR assays were performed by using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA). Each 20 μl reaction mix contained 5 μl of 4×Fast Virus 1-Step Master Mix ...
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bioRxiv - Immunology 2020Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies, CA, USA). The expression of the genes of hexokinase 2 (HK2 ...
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bioRxiv - Immunology 2020Quote: 0.5 - 1 μg of total RNA was prepared by column purification (MachereyNagel) and transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (RevertAid Reverse Transcriptase, Thermo Fisher Scientific, #EP0441) in a total volume of 10 or 20 μl ...
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bioRxiv - Physiology 2020Quote: ... One microgram of RNA was reverse transcribed into cDNA using oligo-dT and MMLV-RT (Moloney Murine Leukemia Virus Reverse Transcriptase, Invitrogen, Thermo Fisher Scientific). Before cDNA synthesis ...
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bioRxiv - Microbiology 2020Quote: ... diluted to 125 ml with virus dilution medium and added to confluent monolayers of Vero-E6 cells in twenty-five T175 flasks (Nunc; 5 ml/flask). After 1 hour of incubation at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Systems Biology 2023Quote: ... and yield was determined by quantitative real-time PCR using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA) and TaqMan Gene Expression Assay ...
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bioRxiv - Synthetic Biology 2024Quote: MetBo2 (polyoma middle T oncogene–induced mouse mammary tumor on a syngeneic Friend Virus B NIH Jackson (FVB) background)[23] cells were maintained in 1X Dulbecco’s Modified Eagle Medium (DMEM) (Thermofisher Scientific; Cat. No. 11995065) with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... lentiviral particles were generated through the transient transfection of plasmid into 293T virus- producing cells (ATCC, Manassas, VA, USA) in the presence of Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA), vesicular stomatitis virus G (VSV-G) ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was used to detect and determine virus titers using the AgPath ID one-step RT-PCR kit (ThermoFisher Scientific, Waltham, MA) from the cell culture supernatants as previously described [28] ...
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bioRxiv - Microbiology 2024Quote: ... A 10 µL RT–qPCR reaction to identify the RNA enteric viruses of interest contained 2.5 µL of fast virus 1-step master mix (4X) (Life Technologies, Carlsbad, CA, USA), 500 nM of each specific primers ...
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bioRxiv - Microbiology 2024Quote: ... half of the virus supernatant after the concentration step was digested with amplification grade DNAse I (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA), for 10 minutes at 37 °C before DNA extraction to reduce host DNA contamination ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed to detect the presence of influenza A virus (M gene) using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific). Positive influenza samples were then selected for whole genome sequencing using next generation sequencing (NGS ...
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bioRxiv - Microbiology 2023Quote: ... Virus detection was performed by quantitative RT-qPCR on a QuantStudio® 5 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA), according toconditions described in Guedes et al ...
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bioRxiv - Cancer Biology 2022Quote: ... 266-6 CAS9 cells were infected with FUW-TetO-KRASG12D-BleoR virus and cells were selected with 5 mg/ml zeocin (Invitrogen, San Diego, CA) for 10 days ...
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bioRxiv - Pathology 2023Quote: ... RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Life Technologies, Carlsbad, CA, USA) on the LC96 instrument (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesis of c DNA from 1000 ng of pooled total RNA was carried out by using 10 μM of reverse primer from each reconstructed RNA virus and RevertAid Reverse Transcriptase (Thermo Fisher scientific, USA) (Cat ...
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bioRxiv - Microbiology 2023Quote: ... in a total volume of 25 µL containing 5 µL of template employing Taqman Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, USA). Oligonucleotide primer and probe sequences and thermocycling conditions are detailed in Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... The infusion line was primed with a loading line solution that was identical to the virus suspension solution [Corning phosphate-buffered saline without Ca2+ or Mg2+ with 350 mM NaCl (Gibco, ThermoFisher Scientific, Waltham, MA) containing 0.001% F68 surfactant (Gibco ...