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Citations for Thermo Fisher :
1451 - 1500 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Plates were scraped and transformant plasmids isolated using the GeneJET plasmid purification kit (Thermo scientific).
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bioRxiv - Synthetic Biology 2020Quote: Yeast plasmid DNA was extracted using the ChargeSwitch Plasmid Yeast Mini Kit (Invitrogen, California, USA) by following the manufacturer’s protocol with minor modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA was extracted from bacteria using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and the obtained concentration was determined by Nanodrop (Life Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... Plasmid isolation was performed with the PureLink HiPure Plasmid Filter Maxiprep Kit (Thermo Fisher Scientific). Insertion was confirmed by DNA sequencing.
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific, Landsmeer, The Netherlands). Oligonucleotide primers (Supplemental Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific, Landsmeer, The Netherlands). PCR products were purified from gel using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNAs used in transfection experiments were purified using the Purelink Plasmid Midi Kit (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... and the plasmids were then extracted using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific). Plasmids were Sanger sequenced on a capillary 3130xL Genetic Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Plasmid DNA was extracted using the GeneJET Plasmid Maxiprep Kit (ThermoFisher Scientific; Cat. No. K0491) for transfections ...
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bioRxiv - Cell Biology 2024Quote: ... by co-transfection of 0.6µg/ml expression plasmid with 0.06µg/ml pCoBLAST selection plasmid (Invitrogen). Cells were grown for 3-5 days before selection with 25µg/ml blasticidin ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pLL3.7m-based plasmids were purified by PureLink Expi Endotoxin-free Maxi plasmid Purification kit (Invitrogen), and then HEK293T (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... The modified pGL4.13 plasmids and pRL-CMV plasmid were co-transfected with Lipofectamine 2000 (Invitrogen) at a mass ratio of 30:1 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were prepared from single colonies using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... with CTL plasmids plus psPAX2 and VSV-G packaging plasmids using Lipofectamine 2000 (Invitrogen 11668019). The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media ...
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bioRxiv - Synthetic Biology 2023Quote: ... Constructed plasmids were extracted using the GeneJET Plasmid Miniprep Kit® (Thermo Fisher Scientific©). All procedures followed the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... These two plasmids and a plasmid conferring puromycin resistance were transfected using LipofectamineTM 3000 (Invitrogen). Cells were selected with 1 μg/ml puromycin (Gibco ...
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bioRxiv - Plant Biology 2024Quote: ... High-purity plasmid DNA was prepared using PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific). Arabidopsis mesophyll protoplast transfection was performed by a polyethylene glycol (PEG)-calcium transfection method (49) ...
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bioRxiv - Microbiology 2024Quote: ... the plasmid was isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, MA, USA). Once obtained ...
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bioRxiv - Neuroscience 2020Quote: ... because we were not able to identify a specific shRNA target sequence using web-based design tools (InvivoGen siRNA Wizard, and Invitrogen Block-iT RNAi Designer). For calcium imaging ...
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bioRxiv - Biochemistry 2019Quote: NPM1 knockdown cell line was generated in AW13516 cell line by transfecting with NPM1 shRNA construct in pTRIPZ vector (Thermofisher Scientific, Waltham, MA, USA) for a period of 24 h followed by antibiotic selection using 1.6 µg/ml puromycin for 4 days ...
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bioRxiv - Microbiology 2020Quote: ... Maxipreps of rescue plasmids were purified using PureLink™ HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Transfection grade plasmid DNA were isolated using the PureLink HiPure Plasmid Miniprep Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... The plasmid was purified using a maxiprep commercial kit (PureLink HiPure Plasmid Filter Maxiprep Kit, Invitrogen). BV2 cells were seeded in 24 well-plates at 70-90% confluence 24 hours prior to the experiment in high glucose DMEM (Gibco ...
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bioRxiv - Genomics 2019Quote: ... cells were transfected with 40 ng renilla plasmid + 400 ng pGL4.23 plasmid using Lipofectamine 2000 (Invitrogen). 48 hours following transfection ...
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bioRxiv - Plant Biology 2019Quote: ... Cas9 and sgRNA plasmids were extracted with the PureLink HiPure Plasmid Midiprep Kit (Invitrogen, cat#K210005). Single stranded salt-free oligonucleotide templates at the length of 42-62 nt were synthesized and dissolved in sterile water at a final concentration of 50 μM ...
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bioRxiv - Biochemistry 2020Quote: ... and plasmid DNA isolated using a PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit (Thermo Scientific). The purified plasmid was finally digested again with HindIII/SnaBI and gel-purified ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmids were harvested from the cultures using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen™). After extraction of the assembled plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected with plasmids (pCAGGS-EGFP and the px333 plasmids) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids used for injection were purified using the PureLink HQ Mini Plasmid DNA Purification Kit (ThermoFisher) using the extra wash step and buffer recommended for endA+ strains ...
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bioRxiv - Cell Biology 2021Quote: ... packaging plasmid psPAX2 (3μg) and envelope plasmid pMD2.G (3μg) using Lipofectamine 2000 Transfection Reagent (Invitrogen). Post 18 hr ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid mutant library were purified from the bacteria colonies using PureLink HiPure Plasmid Midiprep Kit (Invitrogen). All primers in this study were ordered from Integrated DNA Technologies.
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bioRxiv - Genetics 2022Quote: ... Plasmid DNA was extracted from 9 OD600nm units using the ChargeSwitch Plasmid Yeast Mini kit (Invitrogen). Plasmid DNA was adjusted to equal concentrations ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with lentiviral plasmids and packaging plasmids using Lipofectamine 2000 (Thermo Fisher Scientific; 11668019) according to the manufacturer’s instructions in Opti-MEM medium (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... This plasmid was individually co-transfected with pCMV-PBase plasmid into mESCs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... The plasmids of the positive hits were isolated using the GeneJET Plasmid Miniprep Kit (Thermo Scientific) and transformed afresh to the P ...
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bioRxiv - Bioengineering 2019Quote: ... coli cultures were harvested for their plasmid using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Guide plasmid combinations and substoichiometric amounts of dsRed plasmid were cotransfected by Lipofectamine® 2000 (Invitrogen) transfection (375 ng each guide plasmid ...
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bioRxiv - Genomics 2020Quote: The plasmids for dCas9-CTCF and gRNA were extracted using PureLink HiPure Plasmid Midiprep Kit (Invitrogen). For the electroporation ...
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bioRxiv - Immunology 2021Quote: ... packaging plasmid (pCMV-dR8.2) and envelope plasmid (pCMV-VSV-G) using Lipofectamine-LTX reagent (Thermo Fisher Scientific India Pvt ...
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bioRxiv - Immunology 2020Quote: ... The plasmids were isolated from bacterial culture using PureLink(tm) Quick Plasmid Miniprep Kit (Invitrogen, USA) or NucleoSpin® Plasmid EasyPure Miniprep Kit (Macherey-Nagel ...
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bioRxiv - Synthetic Biology 2023Quote: Purification of plasmids was done with the Thermo Scientific GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The pCAS vectors with split-G418 selection were adapted from Bean et al ...
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bioRxiv - Neuroscience 2023Quote: ... Each plasmid was amplified and purified using PureLink HiPure Plasmid Filter Maxiprep Kit (Cat# K210016, Thermofisher).
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bioRxiv - Neuroscience 2023Quote: ... and co-transfected with 1ug transposon targeting plasmid and 1ug transposase plasmid using Lipofectamin3000 (ThermoFisher, L3000015) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was extracted from ten positive clones using the GeneJET Plasmid Miniprep Kit (Thermo Scientific) and Sanger sequenced (EuroFins Genomics) ...
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bioRxiv - Molecular Biology 2023Quote: ... Supercoiled plasmid DNA (pBAD-HisB) was obtained by the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and was added to the 1-2 nM concentration ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid extractions were performed per manufacturer protocol using the GeneJET Plasmid miniprep kit (Thermo Fisher Scientific). All plasmid constructs were confirmed by Sanger sequencing at the Massachusetts General Hospital CCIB DNA Core facility or by Illumina sequence at SeqCenter ...
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bioRxiv - Biophysics 2024Quote: ... Plasmid transfections were performed 24 hours prior to imaging with plasmid DNA in Opti-MEM (Invitrogen) with 5 μL of Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmid purification was performed with a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, MA USA) (Lorenzo et al. ...
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bioRxiv - Molecular Biology 2024Quote: Validated plasmids encoding CBP gRNA and custom synthesised CBP HDR template plasmid (Supplementary Table 1, ThermoFisher) were co-transfected into HEK293T cells using the Neon electroporation system and 100 µl kit (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 488 chicken anti-rat IgG (Invitrogen Molecular probes Cat#A21470); and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).