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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Protein quantification was performed with MicroBCA Assay kit (Pierce BCA protein estimation kit, 23225, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Pierce™ BCA Protein Assay Kit and ECL advanced reagent kit were purchased from Thermo Fisher Scientific (MA ...
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bioRxiv - Cell Biology 2024Quote: ... Live/Dead viability/cytotoxicity kit and Pierce™ BCA protein assay kit were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2020Quote: EVs were isolated from FAPs serum free cell culture medium in parallel by ultracentrifugation (UC) and with Total exosomes isolation reagent -TEIR- (#4478359, Invitrogen by Thermo Fisher Scientific) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The peptide samples were subjected to a LTQ Orbitrap classic for data dependent acquisition and a Q-Exactive for parallel reaction monitoring (Thermo Fisher Scientific) analysis as described previously (Swa et al. ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... four 6.5 µL aliquots of the purified arrays were moved into the interim pDonor4r-3r in parallel by the standard Invitrogen Gateway BP reaction recipe (ThermoFisher 11789-020) which was allowed to remain at room temperature for 72 h prior to stopping the reactions with Proteinase K and transformation of all material into E ...
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bioRxiv - Immunology 2024Quote: ... parallel reaction monitoring (PRM) was performed on an Exploris 480 mass spectrometer coupled with the Vanquish Neo LC system (Thermo Fisher Scientific). A total of 2 μg of digested peptides were separated on a nano capillary column (20 cm × 75 μm I.D. ...
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bioRxiv - Cell Biology 2024Quote: ... parallel reaction monitoring (PRM) was performed on an Exploris 480 mass spectrometer coupled with the Vanquish Neo LC system (Thermo Fisher Scientific). A total of 2 μg of digested peptides were separated on a nano capillary column (20 cm × 75 μm I.D. ...
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bioRxiv - Cell Biology 2020Quote: ... nuclear and membrane lysates using a subcellular protein fractionation kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were developed using the Pierce-ECL Western Blotting Substrate Kit (Thermo Scientific).
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bioRxiv - Plant Biology 2020Quote: ... PVDF membranes were stained with the Pierce reversible protein staining kit (Thermo Scientific).
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bioRxiv - Biophysics 2020Quote: ... The dye was a part of BacLight bacterial membrane potential kit from ThermoFisher Scientific® ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were developed with a Supersignal West femto chemiluminescent substrate kit (Fisher scientific) and developed using Chemidoc (Biorad ...
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bioRxiv - Cell Biology 2023Quote: ... from RNA extracted with silica membrane column purification (PureLink RNA mini kit Thermofisher).
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bioRxiv - Genetics 2023Quote: ... Proteins were transferred onto nitrocellulose membrane with an Iblot machine (kit IB301001, INVITROGEN) using program 3 ...
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bioRxiv - Plant Biology 2024Quote: ... DNA concentration was verified fluorometrically using either the Qubit® dsDNA HS Assay Kit or Quant-iT™ PicoGreen® dsDNA Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Samples were prepared in a similar process to the one described in Illumina’s 16s Metagenomic Sequencing Library Preparation Protocol ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the GeneJET Gel Extraction Kit (ThermoFisherScientific, Vilnius, Lithuania) and quantified using a fluorometric RiboGreen kit (Qubit Assay Kit, Invitrogen, Life Technologies Corporation ...
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bioRxiv - Microbiology 2024Quote: ... The membrane proteins of AGS cells were isolated with the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, United States). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: For microRNA analyses we used mirVanaTM miRNA Isolation Kit and TaqMan MicroRNA Assays (Thermo Fisher, 4437975; Assay ID 001973 (U6), 001940 (miR-675-5p) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were then measured by bicinchoninic acid (BCA) assay (Micro BCA™ Protein Assay Kit, Cat # 23235; Thermo Scientific). The resulting supernatant yielded low volumes (30-40 µl of supernatant ...
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bioRxiv - Cancer Biology 2020Quote: C-circle assays were carried out as previously described .Sample DNA concentrations were determined using the Qubit dsDNA BR Assay kit (ThermoFisher) and isolated genomic DNA was used as a template for rolling circle amplification ...
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bioRxiv - Biochemistry 2021Quote: ... the protein concentration of each fraction was determined using a bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific). Samples were stored at −80 °C until further use.
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bioRxiv - Cancer Biology 2022Quote: ... EdU assays were performed using Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (#C10420; Life Technologies) per manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Peptide concentration was measured with a colorimetric assay (Pierce Quantitative Colorimetric Peptide Assay kit, Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was measured through the Bicinchoninic Acid protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™ 23225). Equal amounts of proteins extracts (20-30 μg for mitochondrial samples or 40 μg for total kidney homogenates ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of EL fractions and TL were determined using the Bicinchoninic acid assay (BCA Protein Assay Kit, Thermo Scientific). Bovine serum albumin was used as a protein standard ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of the cell lysate was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific, USA). The cell pellet was resuspended in 40 μl ddH2O and mixed carefully ...
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bioRxiv - Immunology 2022Quote: In vivo and in vitro proliferation assays were performed using a Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration was calculated by measuring the concentrated proteins with a BCA absorbance assay according to the manufacturer’s instructions (Pierce™ BCA Protein Assay Kit #23225, ThermoFisher).
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bioRxiv - Immunology 2024Quote: Assay was carried out using the ADP-Glo kinase assay kit in 384 Flat White Plates (142761, Thermo Fisher Scientific) as previously described5 ...
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bioRxiv - Microbiology 2024Quote: ... TUNEL stained according to the included assay protocol in the Click-iTTM Plus TUNEL assay Alexa Fluor 594 kit (Invitrogen), and then stained with Hoescht 33342 (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: In vitro proliferation assays were conducted using the Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... More precise and accurate concentration estimates were obtained with a fluorescent-based quantitation assay (QuBit fluorometer, “Quant-It” assays kit, Invitrogen). Concentrations were normalized to 2 nM ...
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bioRxiv - Genomics 2023Quote: ... The RNA amount and integrity were confirmed using the Qubit RNA HS Assay Kit and RNA IQ Assay (Thermo Fisher) respectively ...
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bioRxiv - Bioengineering 2023Quote: The efficiency of the norbornene-thiol reaction was measured using a thiol quantification assay (Measure-iT Thiol Assay Kit, Invitrogen).37 The cell adhesive ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 M sucrose) and a protein concentration assay was performed (BCA Protein Assay Kit, Pierce-Thermo Fisher Scientific, Melbourne, Australia). 100 μg of isolated mitochondria were collected and centrifuged at 12,000 g for 15 min at 4 °C and after removal of the supernatant the pellet was frozen at −80 °C for subsequent proteomics analysis.
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were quantified via a BCA assay using the Pierce BCA Protein Assay Kit obtained from Life Technologies (Catalog #23225). Cell lysates were run through a 4-12% SDS-PAGE gel electrophoresis at 140-170V ...
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bioRxiv - Microbiology 2024Quote: ... the dual luciferase assay was conducted using the Pierce Renilla-Firefly Luciferase Dual Assay Kit (Thermo Scientific; Ref. No. 16185). To determine the expression levels of Furin in the bta-miRNA-16a transfected MDBK and HEK 293T cells ...
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bioRxiv - Physiology 2024Quote: ... The total islet protein content for each secretion assay was measured with Micro BCA™ Protein Assay Kit (ThermoFisher, #23235). Data represent the glucagon or insulin content per µg of protein in each tube.
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bioRxiv - Neuroscience 2024Quote: ... protein concentrations were quantified using a Bicinchoninic acid assay (BCA) with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The lysates were diluted to a concentration of 1.5 μg/μL and stored at -80°C until analyzed by Western blot.
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bioRxiv - Microbiology 2024Quote: ... as determined by bicinchoninic acid (BCA) assay using the Pierce™ BCA Protein Assay Kit (Fisher Scientific AG, Reinach, Switzerland). 1,000 AU of GL cells were cultured in five mL cDMEM at 37°C overnight under a humid nitrogen atmosphere ...
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bioRxiv - Cancer Biology 2024Quote: TUNEL assay was performed using Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection (Fisher Scientific, USA) on whole tumors ...
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bioRxiv - Genomics 2024Quote: ... The RNA amount and integrity were confirmed using the Qubit RNA HS Assay Kit and RNA IQ Assay (Thermo Fisher) respectively ...
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bioRxiv - Cell Biology 2024Quote: ... LDH release was assayed according to manual instructions of the LDH assay kit (CyQUANT LDH Cytotoxicity Assay, Invitrogen, cat #C20301).
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bioRxiv - Molecular Biology 2021Quote: ... AcrB expression levels (using 2 μg membrane fraction determined by a BCA assay) were analyzed by western blotting using an iBind western system (ThermoFisher), with an anti-his-tag mAb (MBL ...
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bioRxiv - Cell Biology 2022Quote: ... with 10 mM succinate and 2 μM rotenone as substrates for the coupling assay was added to each well of the XF96 plate and followed by addition of plasma membrane permeabilizer (PMP, Invitrogen). To assess various parameters of mitochondrial function ...
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bioRxiv - Neuroscience 2024Quote: ... Microglia were labeled for live assays prior to incorporation using a cell membrane dye (Vybrant Cell-Labeling Solutions, Life Technologies). A stably expressing mCherry cell line was utilized for live imaging endothelial cells that was previously developed using pAAVS1-P-CAG-mCh (Addgene) ...
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bioRxiv - Neuroscience 2020Quote: ... Tandem Mass Tags (TMT 10-plex) kits and microBCA protein assay kit were purchased from Thermo Fisher Scientific (Rockford ...
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bioRxiv - Genetics 2021Quote: ... Total protein extract was quantified using the commercial kit (Pierce ™ BCA Protein Assay kit #23225, Thermofisher) to normalize the lipid abundance of the samples ...