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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320, CellBlox monocyte/MAC blocking buffer, Invitrogen #B001T07F01). Cells were stained in an antibody cocktail containing the appropriately titrated antibodies described in Supplementary Table S3 for 20 minutes at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (4 x 300 µL PBST) and primary antibodies were added in 1 : 10000 dilution (anti-IgG Fc cat. 31789, Thermo Fisher; anti-His-Tag cat ...
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were stained directly using a nanobody against ALFA tag coupled to a Abberior Star 635P fluorophore (FluoTag®-X2 anti-ALFA - AbberiorStar635P, NanoTag Biotechnologies GmbH) diluted 1: 500 in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... after which cells were stained for 1 hour at room temperature with AlexaFluor-conjugated secondary antibodies diluted in PBS+10% FCS (Life technologies) and stained with DAPI ...
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bioRxiv - Developmental Biology 2024Quote: ... manually minced and enzymatically digested in an orbital shaker for 2 hours at 37°C in digestion mix (9.2 ml DMEM/F12, 5% FCS, 1% Penicillin/Streptomycin, 25 mM HEPES (Gibco, cat. #15630056) and 300 U/ml Collagenase IV (Gibco ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor 647 anti-human CD137 (clone 4B4-1; dilution 1:100; Thermo Fisher Scientific; Cat no A51019); PE anti-human CD134 (clone OX40 ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... and a mouse monoclonal antibody against human KMO (# 60029-1-Ig 1:1000, Thermo Fisher Scientific, Massachusetts, EUA). The membranes were washed and incubated for 1 h at room temperature with an HRP-conjugated secondary antibody (1:10000 ...
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bioRxiv - Bioengineering 2021Quote: ... MCF10A human mammary epithelial cells (ATCC) were cultured in 1:1 DMEM/F-12 (Thermo Fisher Scientific, USA) media which consists of 2.50 mM L-Glutamine and 15 mM HEPES buffer ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... α-SC35 (described previously98, Mouse 1:1000), α-NIN (Institut curie, Human 1:200), anti-Arl13b (proteintech, Rabbit, 1:4500) α-Rabbit Alexa594 (ThermoFisher, 1:400), α-Mouse IgG1 Alexa488 (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg of total RNA from HEK293 cells or tissue samples was treated with DNAseI Free kit (Thermofisher) followed by cDNA generation using the High capacity cDNA kit (Thermofisher) ...
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bioRxiv - Biochemistry 2020Quote: Dvl2-GFP/dsRed-Axin1 HEK293 cells were seeded at ∼60% confluence in Lab-Tek chamber slides (Nunc 177380) that were pre-coated with 0.1 mg/mL poly-D-lysine (Sigma P6407) ...
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bioRxiv - Biophysics 2019Quote: ... The protein was expressed in HEK293S GnTI− cells (ATCC CRL-3022) grown in Expi293 Expression Media (Life Technologies) to densities 2.5 × 106 cells ml−1 ...
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bioRxiv - Biochemistry 2020Quote: The CR3 headpiece stably transfected HEK293S cells were kept as adhesion cell culture growing in Dulbecco’s Modification of Eagle’s Medium (DMEM) GlutaMAX (Gibco) supplemented with 10 % (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mutations introduced into the δ-subunit were established as stable HEK293 Flp-InTM cell lines (Invitrogen, Paisley, UK), generating a stable cell line for each mutant ...
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bioRxiv - Neuroscience 2020Quote: HEK293 and SH-SY5Y cells (ATCC) were cultured in phenol red-free Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 2 mM L-Glutamine (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: HEK293 or HeLa cells were co-transfected with pBABED and pX335 plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Human Embryonic Kidney 293 (HEK293) cells (ATCC CRL-1573) were cultured in complete growth Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with 10% fetal bovine serum (UCSF Cell Culture Facility) ...
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bioRxiv - Microbiology 2021Quote: HeLa cells (ATCC CCL-2) and HEK293 cells (ATCC CRL-1573) were maintained in DMEM high glucose (Gibco) containing 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant constructs were transfected into HEK293 cells either individually or together using Lipofectamine® 3000 reagent (Invitrogen) following the instructions provided ...
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bioRxiv - Systems Biology 2022Quote: ... HEK293 cells were prepared as 2 × 105 cells/mL in DMEM (Dulbecco s Modified Eagle Media, Life Technologies) + 10% FBS (Foetal Bovine Serum ...
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bioRxiv - Biophysics 2022Quote: ... The Fab version of the antibody fusion (Fab-ShK) was expressed in freestyle HEK293 (293F) cells (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Human embryonic kidney cells (HEK293) were cultured in culture flask (150 cm2) to 80 % confluence in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 50 units/ml penicillin G (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... HELA cells (DSMZ ACC-57) and HEK293 cells (DSMZ ACC-305) were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FCS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293-T cells were seeded into 10 cm plates with DMEM containing 0.5 mg/mL geneticin G418 (Thermofisher). Once the cells reached approximately 80% confluence ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 FcγRII cells (a gift from Dr. Craig Roy) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% heat-inactivated fetal bovine serum (HIFBS ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Flp-In T-Rex cells were co-transfected with pOG44 Flp-Recombinase Expression Vector (Invitrogen™, V600520), and pcDNA5 FRT TO plasmids expressing appropriate miniTurbo gene fusions ...
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bioRxiv - Neuroscience 2019Quote: HEK293 and SH-SY5Y cells (ATCC) were cultured in phenol red-free Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 2 mM L-Glutamine (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with EBV-BAC DNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific, Waltham, MA, USA) followed by hygromycin selection ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA knockdown of AC1 and AC9 expression in HEK293 cells was carried out using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: Two HEK293 cell lines stably expressing mouse STAC3 were generated using the Flp-In T-Rex system (Invitrogen). Host cells ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 Flp-In™ T-REx™ cell lines were generated according to the manufacturer’s instructions (ThermoFisher Scientific). BirA-containing plasmids (0.4 µg) ...
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bioRxiv - Cell Biology 2022Quote: ... Calcium influx was visualized in N2a cells and HEK293 cells using Fluo-4 AM dye (Fisher Scientific, F14201) or the GCaMP6s HEK293 cell line described above ...
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bioRxiv - Genomics 2022Quote: Adherent HEK293 cell lines were grown in 10 mLs of DMEM (ATCC 302002) containing 10% FBS (Gibco 16000044) and 1% Pen/Strep (Gibco 15070063) ...
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bioRxiv - Microbiology 2022Quote: ... as well as the HEK293 cell line were grown in Dulbecco’s Modified Eagle’s Medium F-12 50/50 (DMEM/F-12, GIBCO) supplemented with 10% FBS at 37°C with 5% CO2 in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... Lentivirus production was performed according to the manufacturer’s recommendations in HEK293 E cells using Lipofectamine 3000 (ThermoFisher Scientific). Virus-containing supernatant was collected after 24 and 48 h and combined ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Stable HEK293 cell lines were achieved by antibiotic selection with G-418 (1mg/ml, Gibco-BRL, Paisley, UK) added to the medium 3 days after transfection ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were transfected with pcTBK1-His or pcGSK3B-Flag plasmid using Lipofectamine 2000 (Invitrogen, Waltham, MA, US) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... which then were co-transfected into HEK293 6E cells for expression together following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-6E cells (used for protein expression) were cultured in expression medium (FreeStyle F17 (Gibco, Cat#A13835-01)) supplemented with 1% FBS (Sigma ...
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bioRxiv - Cell Biology 2023Quote: Hek293T and Hek293 Flp-In T-REx cells were maintained without antibiotics in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... HEK293 cells were seeded and grown on poly-lysine-coated Lab-Tek II eight-well chamber slides (NUNC) overnight and transfected with plasmid DNA using the Lipofectamine™ 3000 Transfection System (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... HEK293 cells and MCF7 cells purchased from ATCC were cultured in DMEM (high glucose, Life Technologies Corporation, 11965084) containing 15% fetal bovine serum ...
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bioRxiv - Genomics 2023Quote: ... sgRNA-transduced HEK293-idCas9 cells were cultured in high glucose DMEM medium supplemented with L-Glutamine (Gibco 11965092) and 10% FBS ...