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Citations for Thermo Fisher :
1451 - 1500 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The samples were diluted with four volumes of ammonium bicarbonate buffer (40 mM) and digested overnight at 37°C by adding 1 μl of trypsin protease (Thermo Scientific, USA) (1 μg/μl) ...
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bioRxiv - Cancer Biology 2023Quote: ... Undigested tissue particles were filtered through a 70 μm cell strainer and the single cell suspension was subjected to Red Blood Cell (RBC) lysis using ACK (Ammonium, Chloride, Potassium) lysis buffer (Gibco, NY, USA) for 3-5 minutes on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... The synthesized sgRNAs were precipitated from the reaction using Ammonium acetate Stop Solution as described for the mMessage mMachine Kit protocol (Thermo Fisher Scientific). Cas9-mRNA was in vitro transcribed overnight at 20°C from 400-500 ng XbaI-linearized pT3TS-nCas9n (Addgene ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa PPA14 was extracted using the hexadecyl-trimethyl ammonium bromide (CTAB) method (45) and quantified using the NanoDrop (Thermo Scientific, Nanodrop 2000c) spectrophotometer ...
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bioRxiv - Immunology 2024Quote: ... red blood cells from the splenocytes and draining lymph node cells were lysed by resuspending the cell pellets with 3 mL of ammonium-chloride-potassium (ACK) lysing buffer (Gibco™ A1049201) and incubation for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 50 mM ammonium bicarbonate was added to bring sodium deoxycholate concentration to 1% and 8 μg of LysC/Trypsin mixture (Thermo Fisher Scientific) was added to each sample for overnight digestion at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Beads containing samples were washed with 25 mM ammonium bicarbonate and digested with 0.5 micrograms trypsin (Pierce, Thermo Fisher Scientific, MS Grade) using a CEM microwave reactor for 30 min at 55°C ...
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bioRxiv - Immunology 2024Quote: ... and the bone marrow pellet was resuspended in 1 mL of Ammonium-Chloride-Potassium (ACK) lysis buffer (ThermoFisher Scientific, Catalog No. A1049201) for 20 to 30 seconds to remove red blood cells from the pellet ...
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bioRxiv - Neuroscience 2020Quote: ... and 6.5 µl were analysed by a reversed-phase nano liquid chromatography system (Ultimate 3000, Thermo Scientific) connected to an Orbitrap Velos mass spectrometer (Thermo Scientific) ...
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bioRxiv - Genetics 2021Quote: ... the aqueous phase containing the RNA was extracted and loaded into RNA extraction columns (#K0732, Thermo Scientific). Total RNA was divided into 10 μl reactions containing 5 μg of RNA and was treated for 30 mins with 10 units of DNAse I (#04716728001 ...
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bioRxiv - Developmental Biology 2021Quote: ... peptides were separated using revere phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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bioRxiv - Genomics 2020Quote: ... the upper aqueous phase was recovered and nucleic acids were precipitated by adding 2 μL GlycoBlue (Ambion) and 500 μL isopropanol (incubated at −20°C for 10 min) ...
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bioRxiv - Developmental Biology 2022Quote: LC-MS/MS was carried out by nanoflow reverse-phase liquid chromatography (Dionex Ultimate 3000, Thermo Scientific) coupled online to a Q-Exactive HF Orbitrap mass spectrometer (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: LC-MS/MS was carried out by nanoflow reverse-phase liquid chromatography (Dionex Ultimate 3000, Thermo Scientific) coupled online to a Q-Exactive HF Orbitrap mass spectrometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... and an aqueous phase containing peptides was collected and dried with a SpeedVac (Thermo Fisher Scientific, U.S.A.).
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bioRxiv - Cell Biology 2022Quote: ... Pooled samples were fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For solid phase cultures tryptic soy agar (TSA) was used (TSB+ 1.5% w/v agar, Fisher Scientific)) supplemented with the appropriate antibiotics ...
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bioRxiv - Biophysics 2021Quote: ... Phospholipids were separated on a reverse phase Acclaim C30 column (3 μm, 150 × 2.1 mm (ThermoFisher Sientific)) at a flow rate of 0.060 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... The aqueous phase was removed and mixed with an equal volume of phenol-chloroform (pH 4, Ambion). This solution was vortexed for 30s and centrifuged once more ...
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bioRxiv - Biochemistry 2020Quote: ... the polar and apolar phases were collected and dried in a speed vac concentrator (ISS100. Thermo Scientific). These samples were stored at −80 until analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were separated by reverse-phase chromatography (50 cm long EASY-Spray PepMap columns; Thermo Fisher Scientific) with a 140 min linear gradient of water/acetonitrile ...
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bioRxiv - Cell Biology 2021Quote: Trypsinized peptides were separated via reverse-phase nano-HPLC using an RSLCnano Ultimate 3000 (Thermo Fisher Scientific). The mobile phase consisted of water + 0.1% formic acid as buffer A and acetonitrile + 0.1% formic acid as buffer B ...
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bioRxiv - Microbiology 2020Quote: ... Upon reaching mid-log phase the cells were washed twice with 10% glycerol (Fisher Scientific, G33-1) and electroporated with pKM444 ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from mid-log phase cells grown under the indicated conditions using Trizol (Invitrogen) following manufacturer guidelines ...
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bioRxiv - Biophysics 2022Quote: ... Turbidity of phase-separated samples was then estimated using 96-well NUNC optical bottom plates (Thermo Scientific) on a Multiskan Go (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... All phase contrast images of cultured cells were taken with an EVOS microscope at 10x magnification (Invitrogen), and contain a scale bar of 400 microns in the image.
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bioRxiv - Neuroscience 2022Quote: ... Total proteome and phospho-proteome samples were subjected to high pH reversed phase fractionation (Thermo Scientific, 84868), dried and resolubilised in 0.1% TFA prior to LC-MS/MS ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) then electrosprayed into an Q Exactive Plus Mass Spectrometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: Reverse phase liquid chromatography was done with an Ultra Performance Liquid Chromatography (UPLC) system (Ultimate 3000, ThermoFisher). Lipid extracts of serum from mice were separated on an Accucore C18 150×2.1 ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue neurochemical analytes were eluted in MD-TM MP mobile phase (cat. no. 701332, Thermo Fisher Scientific) under isocratic conditions at 0.6 ml/min for 21 min ...
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bioRxiv - Neuroscience 2021Quote: Peptides were separated by reverse-phase chromatography using a Dionex Ultimate 3000 nanoLC (Dionex/Thermo Fisher Scientific) on a C18 capillary column (Acclaim PepMap100 C18 ...
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bioRxiv - Microbiology 2022Quote: ... the digests were separated by reversed phase column chromatography using an EASY nLC 1000 (Thermo Fisher Scientific) with self-packed columns (OD 360 μm ...
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bioRxiv - Genetics 2022Quote: The total RNA was isolated from 1 mL mid-log phase cultures using TRIzol® Reagent (Invitrogen). The resulting lysate was phase separated with 200 μL chloroform and the total RNA was precipitated with the same volume of ice-cold isopropanol ...
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bioRxiv - Biochemistry 2022Quote: ... The oil phase was washed three times with 100 µl 2 ng/µl salmon sperm DNA (Invitrogen). The aqueous phase was purified and transformed into E ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated by a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptide samples were separated on a C-18 reverse phase column (EASY-Spray PepMap from Thermo Scientific with 25 cm length ...
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bioRxiv - Molecular Biology 2021Quote: ... The tryptic peptides were fractionated by high pH reverse-phase HPLC (Thermo Fisher Scientific, Waltham, MA, USA) and analyzed by LC-MS/MS (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated using a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... LC-MS/MS was carried out by nanoflow reverse phase liquid chromatography (Dionex Ultimate 3000, Thermo Scientific) coupled online to a Q-Exactive HF Orbitrap mass spectrometer (Thermo Scientific) ...
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bioRxiv - Physiology 2021Quote: ... centrifuged at 18,600g and the upper phase was precipitated overnight with 2.7x g of GlycoBlue (AM9515, ThermoFisher). Samples wereμ resuspended in 0.3M acetate buffer (pH=4.5 ...
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bioRxiv - Systems Biology 2021Quote: ... peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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bioRxiv - Cancer Biology 2020Quote: LC-MS/MS was carried out by nanoflow reverse-phase liquid chromatography (Dionex Ultimate 3000, Thermo Scientific) coupled online to a Q-Exactive Plus Orbitrap mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were arrested in log phase by treating with 0.1 µg/mL Colcemid™ (Gibco, #15212- 012) treatment for 45 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: The extract was analyzed using reverse phase HPLC on a Dionex Ultimate 3000 chromatograph (Thermo Scientific, USA) using a Nucleodur HTec C18 VP 125/10 semi-preparative column (Macherey-Nagel ...
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bioRxiv - Immunology 2020Quote: ... The resulting upper phases were combined and subsequently dried in a SpeedVac (Thermo Fisher Scientific, Waltham, US). The dried extracts were dissolved in a mixture of chloroform ...
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bioRxiv - Developmental Biology 2020Quote: LC-MS/MS was carried out by nanoflow reverse phase liquid chromatography (Dionex Ultimate 3000, Thermo Scientific) coupled online to a Q-Exactive HF Orbitrap mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... in the reverse phase using a preconcentration column (75 μm DI × 2 cm, 3 μm, Thermo Scientific) and an analytical column (Acclaim PepMap C18 ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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bioRxiv - Developmental Biology 2022Quote: LC-MS/MS was carried out by nanoflow reverse phase liquid chromatography (Dionex Ultimate 3000, Thermo Scientific) coupled online to a Q-Exactive HF Orbitrap mass spectrometer (Thermo Scientific) ...