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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... PCR plates were read using a QuantstudioTM 7 Flex Real-Tim PCR System (Thermo Fisher Scientific) and results were analyzed using a delta-delta CT method ...
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bioRxiv - Bioengineering 2019Quote: ... and the cells were passaged as single cells every 7 days using TrypLE Express (Life technologies). HUVECs were cultured in endothelial cell growth medium-2 (EGM-2 ...
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bioRxiv - Genetics 2020Quote: ... The fibroblasts were replated 7 days post-infection and cultured in DMEM(Thermo Fisher Scientific,CT119955) with 10% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Then 7 μL of SuperScript Master Mix (4ul 5XSSIV Buffer, 1ul 100mM DTT, 1ul RNaseOUT(Invitrogen) and 1ul SuperScriptTM IV Reverse Transcriptase (200U/ul ...
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bioRxiv - Microbiology 2021Quote: ... 1% Penicillin/Streptomycin and 7% of heat-inactivated fetal bovine serum (FBS) (all from ThermoFisher Scientific). VeroE6/TMPRSS2 cells (NIBSC 100978 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 minute at 60°C)] on a QuantStudio 5 or QuantStudio 7 qPCR machine (Applied Biosystems). For qPCR assessments ...
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bioRxiv - Genomics 2021Quote: ... qRT-PCR was performed on the QuantStudio 7 Flex Real-Time PCR System (Life Technologies Corporation) with Quantitect SYBR I Green (TaKaRa Biotechnology [Dalian] Co.Ltd ...
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bioRxiv - Physiology 2021Quote: ... and we performed qRT-PCR using RNA to Ct kit on Quant Studio 7 (Thermo Scientific) to detect gene differentially expressed in CD140+SCA1+ and CD140+SCA1-.
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bioRxiv - Bioengineering 2022Quote: ... and cultured on Petri dishes for 7 days in Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco 12440053) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... From day 7 and onwards RPMI1640 with B-27 serum free supplement with insulin (Gibco, 17504044) was used and changed every 2-3 days.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was used for relative quantification of cDNA on the ViiA 7 Real-Time PCR System (ThermoFisher) (Primer information included in Table S3).
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bioRxiv - Cancer Biology 2020Quote: ... and finally at 72 °C for 7 min using AmpliTaq Gold PCR Master Mix (Applied Biosystems) with template cDNA equivalent to 15 ng of total RNA and a high concentration (0.75 mM each ...
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bioRxiv - Cancer Biology 2021Quote: ... was described previously.7 Cells were maintained in high-glucose Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Caspase activity was assessed using CellEvent(tm) Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen) following manufactures protocol ...
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bioRxiv - Genomics 2021Quote: ... Laser Capture Microdissection (LCM; N=3; 7 µM glass slide coated with polyethylene naphthalate – ThermoFisher #LCM0522), multiplex or regular immunohistochemistry (N≥3 4 µM glass slide ...
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bioRxiv - Genomics 2020Quote: We performed qPCR on a QuantStudio 7 Flex Real-Time PCR instrument (Life Technologies, Carlsbad, CA) using the following conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were mounted on slide glasses (Matsunami) with 7 μL of Permafluor mountant (Thermo Fisher Scientific) for each cover glass.
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bioRxiv - Cancer Biology 2022Quote: ... After 7 days the spheroids were incubated with DRAQ5 (62254, Thermo Fisher Scientific, Waltham, MA, USA) at 1μM for 15 minutes prior to imaging as described (50) ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was synthesized (Maxima H cDNA kit) and qPCR was performed (QuantStudio 7 flex, Applied Biosystems). Primers used for gene expression analysis were ...
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bioRxiv - Cell Biology 2022Quote: COS-7 (ATCC® CRL-1651™) cells were grown in DMEM medium (Thermo Fisher Scientific) supplemented with 10% FBS (fetal bovine serum ...
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bioRxiv - Cell Biology 2022Quote: ... MCF-7 cells were also supplemented with 1x NEAA (non-essential amino acids) (#11140-035, Gibco). Human male diploid lung WI-26 SV40 fibroblasts (WI-26 cells ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: COS-7 cells were transiently transfected with the relevant DNA constructs using Lipofectamine 2000 (Invitrogen, 11668). Cell lysates (1 mg ...
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bioRxiv - Synthetic Biology 2022Quote: ... following manufacturer’s recommendations in triplicate on a QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher). Primer sequences are listed in Supplementary File 2 – Primer List.
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bioRxiv - Microbiology 2022Quote: ... 24 hours and 7 days were labelled with 0.5 μM BODIPY FL (Thermo Fisher, Waltham, MA), a hydrophobic dye that stains lipids ...
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bioRxiv - Plant Biology 2022Quote: RT-qPCR experiments were performed with three biological replicates using a QuantStudio 7 cycler (Applied Biosystems). The 10 μl reaction mixture contained 5 μl 2X SYBR Green/ROX master mix (Applied Biosystems) ...
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bioRxiv - Physiology 2023Quote: ... The homogenate was then sonicated 10 times on setting 7 (Sonic Dismembrator Model 100, Fisher Scientific) and incubated at 4°C while rocking for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... All RT-PCR assays were done using the Viia 7 Real-time PCR System (Applied Biosystems). A standard curve was generated in order to obtain the exact number of copies in the tested sample ...
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bioRxiv - Synthetic Biology 2022Quote: ... The concentration of the transcription was measured as 7 µM using a Qubit RNA assay (ThermoFisher). A splint oligonucleotide (Z_Splint ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The RT-PCR reaction was performed using a QuantStudio™ 7 Flex system (Thermo Fisher Scientific) and data analysis was performed using the manufacturer’s web-based software (https://geneglobe.qiagen.com/analyze) ...
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bioRxiv - Molecular Biology 2022Quote: ... All qPCR analyses were performed on an Applied Biosystems QuantStudio 7 Flex Cycler (Thermo Fisher Scientific), and relative expression values were calculated using the comparative CT method (Schmittgen and Livak 2008).
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bioRxiv - Immunology 2022Quote: ... Thermal cycling and fluorescence detection were performed using the ViiA 7 real-time PCR system (ThermoFisher) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... qRT-PCR were run in a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative quantification was determined using the ΔΔCt method and normalized to the endogenous controls GAPDH and RPLP0 ...
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bioRxiv - Molecular Biology 2023Quote: ... or 15 nM siRNA Control using 7 ul of Lipofectamine™ RNAiMAX transfection reagent (ThermoFisher Scientific) for each well according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was carried out using a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) and KAPA SYBR Fast qPCR reagents (KAPA Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Analysis of qPCR amplifications was performed with QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using the 2-ΔΔCT method with 36B4 serving as an internal control ...
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bioRxiv - Cancer Biology 2022Quote: ... and reactions performed on a ViiA− 7 Real-Time PCR System (all, Applied Biosystems, Waltham, MA). Data were acquired using the system’s RUO software and analyzed using the 2−ΔΔCT method ...
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bioRxiv - Developmental Biology 2023Quote: ... testicular tissues were digested with 7 ml collagenase type IV (290U/ml, Gibco cat # 17104-019) for 10 min at 37 °C with gentle agitation (250 rpm ...
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bioRxiv - Biophysics 2023Quote: ... and Septin-7 (VectorBuilder, Cat# VB220525-1339dzw) was performed in Opti-MEM (Gibco, Cat# 31985-070) using SuperFect reagent (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were run in a ViiA 7 Real-Time PCR system (Applied Biosystems, Foster City, CA) using fast reaction presets ...
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bioRxiv - Neuroscience 2023Quote: ... the hiPSCs were maintained for 7 days in a medium containing Neurobasal (Thermo Fisher Scientific, 12348017) and 1% neural induction supplement (NIS – Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... All qPCR reactions were run in triplicate and used SYBR-green on a QuantStudio 7 (ThermoFisher). The 2^(-ΔΔCt ...
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bioRxiv - Genetics 2023Quote: ... and 7 μM sections were cut using a cryostat onto Colorfrost Plus slides (Thermo Fisher Scientific). Sections were permeabilized in 0.25% Triton X-100 in PBS at room temperature for 12 min ...
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bioRxiv - Developmental Biology 2023Quote: COS-7 and HEK293 cells were grown in on plastic dishes in high-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was run in a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific 4453545) with cycle settings following manufacturer’s protocols for the RT-qPCR kit ...
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bioRxiv - Physiology 2023Quote: ... followed by 7 days of decalcification with Shandon’s TBD-2 (Thermo Fisher Scientific, Waltham, MA, USA) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split every 5-7 days by incubation with 0.5 μM EDTA (Thermo Fisher Scientific) for 4 min at room temperature and clumps were dissociated into small clumps by pipetting ...
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bioRxiv - Biochemistry 2022Quote: ... These SCX fractions (n=7) were cleaned up using C18 spin columns (89870, Pierce, ThermoFisher, USA), dried using SpeedVac at 40 °C and resuspended in formic acid (0.1 % ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT using ‘SnakeSkin’ dialysis tubing with a 7 kDa molecular weight cut-off (ThermoFisher). His6-tagged cleavage products as well as TEV protease were removed with Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... Real-time qPCR was performed in a QuantStudio 7 Flex real-time PCR system (Thermo Fisher) using iQ SYBR Green Supermix (Bio-rad #1708880 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cytotoxicity of CellTrace™ Far Red-labelled tumor cells was measured by 7-AAD staining (Invitrogen) and flow cytometry ...