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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The medium was changed every 2-3 days and organoids were passaged every 2-4 weeks by dissociation with 1 ml of TrypLE Express (Life Technologies) at 37°C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... CAT-1 (encoded by SLC7A1) and CAT-3 (encoded by SLC7A3) had 4 substrates including L-Lysine hydrochloride (Fisher Scientific #BP386), L-Histidine (Sigma-Aldrich #H6034) ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were incubated for 20 min at 37°C in PBS containing 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; 1 μM; Thermo Fisher Scientific). To determine neutral lipid content ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed extensively with PBS and activated via photoirradiation (365 nm, 0.8 mW for 5 minutes) with sulfosuccinimidyl-6-4’-azido-2’-nitrophenylamino hexanoate (Sulfo-SANPAH; Thermo Scientific Pierce). To do so ...
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bioRxiv - Immunology 2024Quote: ... For T cell panels (Supplemental table 2, 4, 5, 6) we fixed cells with the FOXP3 Fixation/Permeabilization Buffer Kit (Thermo Fisher) and conducted intracellular/nuclear stains using the FOXP3 Permeabilization Buffer (Thermo Fisher ...
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bioRxiv - Bioengineering 2019Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid;D3823, Thermo Fisher Scientific), was added to the culture medium for a duration of 60 min and pumped through the media systems at 80 µl/h via positive pressure provided by a syringe pump ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418 ...
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bioRxiv - Molecular Biology 2019Quote: ... was prebound for 6 h to protein G Dynabeads (Thermo Fisher Scientific) and then added to the diluted chromatin for overnight immunoprecipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was stained with To-Pro-3 (1/200 dilution, 1 mM, Invitrogen T3605).
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bioRxiv - Neuroscience 2022Quote: ... dried and coverslipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Primary antibodies with the following dilutions used in this study include ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... columns were washed with 3 column volumes (CV) of 1:1 RNase ZAP (Ambion) followed by 3 CV nuclease-free water (DEPC-treated MilliQ water) ...
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bioRxiv - Pathology 2024Quote: ... and 3-hydroxybutyrate dehydrogenase 1 (15417-1-AP, Thermo Fisher Sci, Waltham, MA, USA). HRP-conjugated secondary antibodies (NA9340 ...
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bioRxiv - Neuroscience 2022Quote: One-step quantitative real-time PCR was performed in a QuantStudio 6 (Thermo Fisher) using GoTaq® Probe 1-Step RT-qPCR System (Promega) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The cells were passaged at a proportion of 1:8-1:10 with trypsin (Life Technologies, #25200-056) every 3 days or when they reached 80% confluence.
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bioRxiv - Microbiology 2024Quote: ... phase was transferred to a new PLG tube to which another 1 volume equivalent of phenol:chloroform:isoamyl alcohol 25:24:1 (pH 8, Invitrogen) was added ...
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bioRxiv - Developmental Biology 2020Quote: ... (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY® F C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) was purchased from Molecular Probes (Melbourne, VIC, Australia). The TiO2 was collected from a disassembled column ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 2 mM caspase-3/7 detection reagent (Fisher Scientific) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... Apoptosis was detected using the CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher). Frames were captured over a period of 24 hrs at 1 hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius) ...
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bioRxiv - Immunology 2020Quote: ... Apoptosis was detected using the CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher). Frames were captured over a period of 24hrs at 1-hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius) ...
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bioRxiv - Immunology 2021Quote: ... In vitro derived PCs were stained with CellEvent Caspase 3/7-Green (ThermoFisher), tetrametylrhodamine ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with CellEvent Caspase 3/7 Detection Reagent (ThermoFisher Scientific, cat. no. C10423) to a final concentration of 2 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... Cytotoxicity was assessed using the CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at 1.0 µM and gating on CD45-negative cells ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific). Images were captured automatically every two hours for 48 hours using the IncuCyte™ S3 Live-Cell Analysis Instrument (Essen BioScience) ...
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bioRxiv - Cell Biology 2023Quote: ... and treated with 2 µM Caspase-3/7 Green detection reagent (C10423, Invitrogen) along with the specified compounds ...
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bioRxiv - Cancer Biology 2024Quote: ... The obtained cell lysates were spin for 10 min of centrifugation at 20,000 × g at 4°C and the collected cell lysate supernatants were pre-absorbed with C-1 magnetic beads (Thermo Fisher Scientific, #65002) for 1 h at room temperature before proceeding to oligo probe hybridization ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM of 4-(2-hydroxyethyl)-1-piperazine-1-ethanesulfonic acid (HEPES) (Gibco) and 1 ng/mL of human bFGF (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (1 M) (Invitrogen, cat.#15630080), CellMask membrane dye (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... whole cell lysate was collected by centrifugation at 13,000 × g for 15 min at 4 °C and treated with 5 U/ml RNase T1 (Thermo Fisher Scientific) and Turbo DNase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... then the supernatant was diluted 10x and pre-cleared for 30 min at 4 °C with rotation using 5 µl protein A- and G-coupled dynabeads (ThermoFisher Scientific). An input sample was taken ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were cleared by centrifugation at 16,000 × g for 5 min at 4°C and protein concentration was determined using the Pierce BCA protein assay kit (Thermo Scientific). Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar ...
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bioRxiv - Immunology 2023Quote: ... cells were pelleted at 100 x g for 5 min (4°C) and then again digested with TrypLE Express Enzyme (Gibco, 12604013) for 15 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-phosphorylated antibodies AT-8 (pSer202/pThr205, MN1020, Invitrogen, 1:50) and AT-100 (pThr212/pSer214 ...
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bioRxiv - Neuroscience 2021Quote: ... All media mentioned for Day 1-8 contained 1x penicillin/streptomycin (Gibco), 1 μg/mL laminin (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL of 8 M GuHCl solution (Acros Organics, 50-01-1) was added to each sample and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... pre-coupled to 8 µg anti-CD44 (Fisher Scientific, 15675-1-AP) with BS3 crosslinker (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... for 8 hours and harvested in 1 ml TRIzol reagent (Thermo Fisher). Total RNA was isolated according to the manufacturer’s recommendation ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with Essential 8 Flex supplement and 1% pen/strep (Thermofisher Scientific). The medium was also supplemented with 50 μg/ml G418 (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (4.4 g/L MS salts mixture, 10 g/L D-sucrose) in 6 well-plates (Nunc) and allowed to acclimate for 48 hours with mild agitation (130 rpm).
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bioRxiv - Plant Biology 2019Quote: ... 50 mg mL−1 Serva Blue G) and applied to the precast native 3-12% Bis-Tris polyacrylamide gel (Invitrogen, Life Technologies Corporation, Carlsbad, CA). Blue Native gel electrophoresis was run according to Rantala et al.52 ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were hybridized with a telomere-specific probe G-rich PNA probe Cy3-(TTAGGG)3 (Panagene, Daejeon, South Korea) followed by counterstaining with YOYO™-1 Iodide (Thermo Scientific, Bordeaux, France) according to the TeloSizer® assay procedure ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, F9-6 x 1000 LEX rotor, Thermo Fisher Scientific, Waltham, MA). The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, F9-6 x 1000 LEX rotor, Thermo Fisher Scientific, Waltham, MA). Cells were washed once with water and suspended in 75 mL of ice-cold water ...
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bioRxiv - Immunology 2021Quote: ... 1 μg/ml -CD28 and 1:50 diluted conditioned supernatant from COS-7 cells transfected with a pcDNA1 vector (Invitrogen) encoding for recombinant chicken IL-2 (a kind gift from prof ...