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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 6 O TOLYL IMIDAZO 2 1 B THIAZOLE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and immersed in Hanks’ Balanced Salt solution w/o ions (HBSS w/o Ca2+ and Mg2+; #14175-053, Thermo Fisher Scientific) on ice ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/mL)-coated 6-well plates in hECSR medium (Human Endothelial SFM, Thermo Fisher, cat# 11111-044; B-27 Supplement (50×), Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Pf3D7 and PfKelch13R539T strains were cultured at 2% hematocrit in O+ erythrocytes (Rotary Blood Bank) in RPMI 1640 medium (Invitrogen, USA), supplemented with Albumax I (0.5%) ...
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bioRxiv - Microbiology 2024Quote: ... falciparum strains were cultured at 2% haematocrit in O+ erythrocytes and RPMI-1640 medium (Lonza, BE12-167F or Gibco, 31870-025) supplemented to give the following final concentrations ...
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bioRxiv - Neuroscience 2022Quote: BDA was visualized with fluorophore-conjugated streptavidin (Thermo Fisher Scientific, Table 2; 1:1,000 for 3 h). The reaction was enhanced using the biotinylated tyramine (BT)-glucose oxidase (GO ...
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bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
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bioRxiv - Cell Biology 2021Quote: ... HCT8 cells were grown on a 6-well slide (3×105/well, Thermo Scientific) overnight ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells (3×105) were seeded in a 6 well plate (Nunc,Thermo,USA) with DMEM media (Invitrogen,USA ...
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bioRxiv - Cancer Biology 2019Quote: ... Spheroids pictures were taken after 3 and 6 days using EVOS FL microscope (Thermofisher) and the images were analyzed with ImageJ software ...
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bioRxiv - Cell Biology 2021Quote: ... total 2 μg was diluted into 6 μl of reagent (1:3 ratio) in the 100 μl of Opti-MEM I medium (31985062, Thermo Fisher Scientific) and incubated for 15 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were rinsed with PBS (3×10min) and incubated for 6 hours with secondary donkey anti-rabbit 488 antibodies (1:1000, ThermoFisher Scientific, A11006) and streptavidin 594 (1:750 ...
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bioRxiv - Microbiology 2021Quote: ... in 1X of 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Invitrogen, USA) for 35 min at 180V ...
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bioRxiv - Bioengineering 2022Quote: ... collagen from rat tail (2 mg/ml in hydrochloric acid; Gibco, US) was mixed with 10xMEM (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 2% (w/v) glucose and non-essential amino acids (Gibco), pH 7.2 (abbreviated as RPMI 2% glucose hereafter ...
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bioRxiv - Immunology 2023Quote: ... 2 mM Hepes and 0.1 mM non-essential amino acids (all Gibco) at 37°C with 5% CO2.
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bioRxiv - Biochemistry 2023Quote: ... 2-hydroxyazobenzen-4’-caryboxylic acid (HABA)-avidin complex was used (Thermo Fisher). Quantification was measured at 500 nm ...
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bioRxiv - Biophysics 2023Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes). Stained sections were imaged using epifluorescence and deconvolution epifluorescence microscopy on an Olympus IX83 ...
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bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
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bioRxiv - Neuroscience 2019Quote: ... Inducible overexpression of NGN2 began on day 1 by transferring cells into D0-2 induction medium (DMEM/F12, 1% Pen/Strep, 1x non-essential amino acid solution (NEAA, Gibco), 1% (v/v ...
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bioRxiv - Cell Biology 2019Quote: The mRFP-CLASP2γ U2OS cells expressing H2B-GFP were cultured in glass coverslips using DMEM without phenol red and supplemented with 25 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco) and 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA products at various points of the iTP-seq protocol were analyzed on 9% acrylamide (19:1) TBE (90 mM Tris, 90 mM boric acid, and 2 mM EDTA) gels and stained with SyBR Gold (Invitrogen). Inverse toeprints were excised from 12% acrylamide TBE gels using a clean scalpel ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Penicillin-Streptomycin and 1% nonessential amino acids (All Gibco).
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bioRxiv - Microbiology 2021Quote: ... 1% non-essential amino acids and 1% sodium pyruvate (Gibco), and grown at 37°C and 5% CO2 as described previously (Hoffmann et al 2020) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% nonessential amino acids and 1% penicillin/streptomycin (Life Technologies). Human embryonic kidney (HEK293T ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Cell Biology 2022Quote: ... HCEnCs were rinsed in DPBS and passaged at a ratio of 1:2 or 1:3 with TrypLE (Thermo Fisher Scientific) for 10-15 min at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Microbiology 2023Quote: ... Protein-nucleic acid mixtures were then loaded into either 6% or 10% Novex TBE gels (Thermo Fisher). Gels were pre-run for at least 30 min at 100V in 0.5x TBE buffer before sample loading ...
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bioRxiv - Cell Biology 2020Quote: ... gradient of 2% to 25% buffer B (0.1% formic acid in acetonitrile) was performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). The ion source was operated at 2.4kV and the ion transfer tube was set to 300°C ...
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bioRxiv - Microbiology 2019Quote: ... and solvent B (30% (v/v) Optima LCMS-grade methanol in Optima LCMS-grade water with 0.05% trifluoroacetic acid) (Fisher Scientific). Muropeptides were eluted from the column with a gradient of increasing solvent B (1 min hold at 1% B ...
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bioRxiv - Cancer Biology 2020Quote: ... and solvent B (0.1% formic acid in ACN) over 120 minutes using a Dionex UltiMate 3000 Rapid Separation nanoLC (ThermoFisher Scientific). MS data were obtained on an Orbitrap Elite Mass Spectrometer (Thermo Fisher Scientific Inc ...
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bioRxiv - Microbiology 2020Quote: ... The peptides were separated by increasing concentrations of buffer B (80% acetonitrile / 0.1% formic acid) for 158 min and analyzed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) operated in data-dependent acquisition mode using in-house ...
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bioRxiv - Cell Biology 2020Quote: ... gradient of 2% to 25% buffer B (0.1% formic acid in acetonitrile) was performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). The ion source was operated at 2.4kV and the ion transfer tube was set to 300°C ...
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bioRxiv - Cell Biology 2022Quote: ... 2% solvent B (solvent B: 80% acetonitrile containing 0.1% formic acid) and directly sprayed into a Q-Exactive Plus mass spectrometer (Thermo Scientific) for the data acquisition ...
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bioRxiv - Microbiology 2019Quote: ... The peptides were separated by increasing concentrations of buffer B (80% acetonitrile / 0.1% formic acid) for 158 min and analyzed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) operated in data-dependent acquisition mode using in-house ...
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bioRxiv - Cell Biology 2020Quote: ... Analysis of a 150 min gradient of 2% to 25% buffer B (0.1% formic acid in acetonitrile) was performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). The ion source was operated at 2.4kV and the ion transfer tube was set to 300°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were eluted by a 156 min non-linear gradient of solvent B (0.1% v/v acetic acid in acetonitrile) and injected online in an Orbitrap Velos (Thermo-Fisher Scientific). The survey scans were acquired in the Orbitrap (300–1700 Th ...
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bioRxiv - Cell Biology 2023Quote: ... using buffer A (0.1% formic acid, FA) and buffer B (95% ACN, 0.1% FA) and an Orbitrap Eclipse Tribrid MS (Thermo Fisher Scientific). All fractions were redissolved in buffer A (0.1% FA ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... in millipore water (eluent A) to 0.1% formic acid in acetonitrile (LC-MS grade, Fisher Scientific, Loughborough, UK; eluent B) at a flow rate of 0.5 ml min-1 ...
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bioRxiv - Neuroscience 2024Quote: ... solvent B = 0.1% formic acid in acetonitrile) with the use of a Dionex Ultimate 3000 HPLC System (Thermo Fisher Scientific). Eluted peptides were sprayed with a nano-electrospray source and with a column oven set at 42°C (AMR ...
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bioRxiv - Neuroscience 2024Quote: ... solvent B = 0.1% formic acid in 80% acetonitrile) with the use of UltiMate 3000 RSLCnano LC System (Thermo Fisher Scientific). The Q Exactive HF-X (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... After two washing steps in phosphate-buffer saline w/o calcium and magnesium (PBS w/o Ca and Mg, Thermo Fisher Scientific) and optional erythrocyte lysis (Quiagen ...
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bioRxiv - Neuroscience 2022Quote: ... The final pellet was resuspended in 0.5 mL of NRB containing 6 μM 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher; D1306). The suspension was filtered through a 20 μm filter (Sysmex ...
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bioRxiv - Neuroscience 2019Quote: ... 1 ml of B-27 Supplement (GIBCO, ThermoFisher Cat. #17504044); 1 ml of Penicillin-Streptomycin solution (GIBCO ...
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bioRxiv - Neuroscience 2019Quote: ... 1 ml of B-27 Supplement (GIBCO, ThermoFisher Cat. #17504044); 1 ml of Penicillin-Streptomycin solution (GIBCO ...
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bioRxiv - Bioengineering 2020Quote: ... 1% penicillin-streptomycin-amphotericin B (ThermoFisher Scientific, Waltham, MA; 15240062), 1% Glutamax (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1% penicillin-streptomycin and 1x B-27 Plus (Gibco, A3582801) for 4 h before a complete media change to maintenance medium (plating medium devoid of serum).