Labshake search
Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 4 Nitrophenyl beta D glucuronic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... 0.5 mM aminocaproic acid and 30% sucrose) and loaded onto a 4-16% precast BN-PAGE gel (Life Technologies). Second dimension analysis was performed by solubilizing BN-PAGE gel slices in 2x Laemmli buffer (Laemmli ...
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bioRxiv - Bioengineering 2022Quote: ... glacial acetic acid) for 2 hours at room temperature (Section 2.14) or methanol-free 4% formaldehyde (ThermoFisher, Section 2.16) and then kept in PBS at 4°C before washing in gradations of ethanol up to 100% ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Microbiology 2020Quote: ... were quantified using an avidin and 4’-hydroxyazobenzene-2-carbocylic acid assay according to the manufacturer’s instructions (Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... 1,1’-bi(4-anilino) naphthalenesulfonic acid (Bis-ANS) and Alexa Fluor® 488 phalloidin were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Immunology 2021Quote: ... Uptake of fatty acids was quantified after incubation with 1uM 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic acid (Bodipy-FL C16, Thermo Fisher) at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were acidified with 4 μL of 50 % formic acid and dried in a SpeedVac centrifugal evaporator (ThermoFisher) at 65°C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Cancer Biology 2023Quote: BODIPY FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Invitrogen D3821) was used to assess the ability of 3T3s to transport fatty acids into the cell from their surroundings ...
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bioRxiv - Bioengineering 2024Quote: ... PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Neuroscience 2024Quote: ... BODIPYTM-C12 500/510-C1 (4,4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid; Invitrogen) or BODIPYTM-C12 558/568 (4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid ...
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bioRxiv - Cell Biology 2024Quote: BODIPY-FL-C16 (C16) (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid (ThermoFisher, #D3821) or BODIPY 558/568 C12 (C12 ...
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bioRxiv - Developmental Biology 2023Quote: ... We washed the cumulus-oocyte complexes in Tissue Culture Medium-199 with Hanks salts supplemented with 25mM HEPES followed by in vitro maturation in Tissue Culture Medium-199 with Earle salts (Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2020Quote: ... A beta release of the Collibri 3’ mRNA Library Prep Kit (Invitrogen) was used to prepare libraries ...
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bioRxiv - Bioengineering 2023Quote: ... and housekeeping gene for beta tubulin (TUBB) (#Hs00742828_s1, ThermoFisher Scientific, Waltham, MA). TaqMan master mix (4444556 ...
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bioRxiv - Immunology 2022Quote: ... Pre-designed gene expression assay for beta-Actin (Applied Biosystems™, Mm01205647_g1) was used for normalization ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% penicillin/streptomycin (Pen/Strep) and 0.5 μM beta-mercaptoethanol (Life Technologies). Medium was changed every second day and cells were passaged at 1:3 or 1:5 ratio ...
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bioRxiv - Microbiology 2024Quote: ... sheep polyclonal anti-dopamine beta hydroxylase (DBH, Thermo Fisher Scientific, 1:50); Tuj1 mouse anti-tubulin-β-III (Tuj1 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cat.# 25249-1-AP)28 and anti-beta-actin antibody (Life technologies, Cat.# MA5-15739-HRP) as the housekeeping control.
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bioRxiv - Cancer Biology 2024Quote: ... ELISA was performed using IL-1 beta Human ELISA Kit (Invitrogen #KHC0011) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... anti-beta-2-microglobulin antibody (1:100, MA5-36022, Thermo-Fisher Scientific), anti-HLA-I-ABC antibody (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 ng ml−1 heregulin beta-1 (Thermo Fisher 100-03-50UG), 1 μM CHRI99021 (Tocris 4423) ...
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bioRxiv - Microbiology 2024Quote: ... and 4 μl was spotted onto LB agar or on LB agar with 1% (w/v) sodium deoxycholate (DOC, Thermo Fisher Scientific), as has been previously described (56) ...
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate Bis-Tris polyacrylamide gel electrophoresis (SDS-PAGE) (NuPAGE™ 4 to 12%, Bis-Tris precast polyacrylamide gel, Invitrogen # NP0323BOX) and afterward transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate (cat # 11885084, Life Technologies Inc.). The tumor stromal organoid was incubated for 3-4 days in RGF BME coated plates to establish cancer dormancy ...
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bioRxiv - Cancer Biology 2022Quote: The detection of TGF-β in the cell culture media from SH-SY5Y and MDA-MB-231 cells treated with TEPA for 24h was performed according to the protocol suggested by Thermo Fisher (TGF-beta-1-Human-ELISA-Kit/BMS249-4).
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bioRxiv - Biochemistry 2023Quote: ... DNA fragment encoding TwCel5CBM was cloned (30 - 412 amino acid residues) into the directional champion pET151/D-TOPO™ expression vector (Invitrogen, Carlsbad, CA), which adds a cleavable N-terminal V5-6xHis-tag to the protein as per manufactures instructions.
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bioRxiv - Biochemistry 2020Quote: ... acetic acid and formic acid were purchased from Fisher Scientific, Dithiothreitol ...
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bioRxiv - Microbiology 2023Quote: ... Dipicolinic acid and nitrilotriacetic acid were purchased from Fisher Scientific. Captopril was purchased from VWR ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C) and dialyzed using Slide-A-Lyzer Dialysis Cassettes (3,500-D protein molecular weight cutoff; Fisher Scientific, 68035) against buffer D (20mM Hepes-KOH pH 7.9 ...
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bioRxiv - Molecular Biology 2020Quote: ... fixed iAT2s were either stained for cell surface expression of ACE2 (R&D, #AF933, 4-8μg/2.5×106 cells) followed by donkey anti-goat IgG-AF647 (Invitrogen, #A21447) or were permeabilized with saponin buffer (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... CCR10 (248918) from R&D, CD11a (M17/4) from eBioscience, FcεRI (MAR-1), c-Kit (CD117, 2B8) from Invitrogen and Mouse BD Fc Block™ ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% n-Dodecyl-β-D-maltoside were loaded onto Novex 4-16% Bis/Tris gradient gels (Life Technologies). When assaying stoichiometry of AMPAR-auxiliary subunit complexes ...
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bioRxiv - Immunology 2022Quote: ... filtered through a 70μm cell strainer and centrifuged at 4°C for 10min at 400xg before resuspending cells in D-PBS (Gibco) determining cell counts and viability using a nucleocounter® ...
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bioRxiv - Immunology 2024Quote: ... or stimulated with anti-CD3/CD28 Dynabeads at a 4:1 bead:cell ratio (Fisher Scientific, cat#: 11-452-D).
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bioRxiv - Neuroscience 2024Quote: ... 5% CO2 in GFD medium supplemented with 5 mM L-glucose and 1 mM 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG; Invitrogen). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell debris was pelleted by centrifugation at 13,000g for 10 minutes at 4°C and the protein concentration measured by bicinchoninic acid (BCA) assay (Pierce, Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16, Thermo Fisher Scientific) in a modified SC medium ...
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bioRxiv - Physiology 2020Quote: ... EDL from both legs were dissected and maintained in 4°C Krebs–Henseleit buffer supplemented with 1×MEM amino acid mixture (Invitrogen) and 25mM glucose ...
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bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Biochemistry 2024Quote: ... Cytosolic and nuclear extracts and acid-extracted histones were separated on 10% or 4-12% Bis-Tris gels (Life Technologies) and transferred to nitrocellulose membranes (0.2 μm ...
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bioRxiv - Genetics 2024Quote: ... pH 8.2 by beating with 0.5 mm glass beads at 4°C and protein concentration was determined by bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Protein was reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were centrifuged at 13,000 x g at 4℃ for 20 min and protein concentrations in supernatant were determined by bicinchoninic acid assay (Thermofisher) as per the manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... For buffered growth mDMEM-DMP was supplemented with 100 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco 15630056).
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bioRxiv - Cell Biology 2024Quote: ... or BODIPY 558/568 C12 (C12) (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (ThermoFisher, #D3835) were complexed with fatty acid-free bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... and buffer salts were obtained from Fisher Scientific (Pittsburgh, PA) and Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... 10 mL of Hank’s balanced salt solution without calcium (Gibco) was added ...
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bioRxiv - Biophysics 2021Quote: ... and buffer salts were obtained from Fisher Scientific (Pittsburgh, PA) and Sigma-Aldrich (St ...