Labshake search
Citations for Thermo Fisher :
101 - 136 of 136 citations for pG2 er since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... we selected a few small volumes containing mitochondria and manually classified the voxels for the ER using Amira (ThermoFisher). We used a modified watershed algorithm to classify the mitochondrial membranes but performed a manual curation to remove artifacts ...
-
bioRxiv - Cell Biology 2024Quote: ... The absorption spectra of ER-Tracker and Mito-Tracker were measured using a UV-VIS spectrometer (GENESYS 50, Thermofisher).
-
bioRxiv - Immunology 2023Quote: ... plasmids encoding for various SCTs were co-transfected with a plasmid encoding for an ER-retained BirA-ligase into FreeStyle 293-F cells (Invitrogen) using the 293-free transfection reagent (Merck ...
-
bioRxiv - Microbiology 2023Quote: ... The MCF7 (ER+, PR +/-, HER2 0, Ki-67 90%) (Subik et al., 2010) cell line was maintained in Minimum Essential Media (Gibco) supplemented with 10% fetal bovine serum (Atlanta Biologics) ...
-
bioRxiv - Cell Biology 2023Quote: ... the COS-7 cells were transfected with plasmids encoding tubulin (3×mEmerald-ensconsin) and ER (mCherry-KDEL) by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol17 ...
-
bioRxiv - Plant Biology 2023Quote: ... were co-transformed with the plasma membrane marker PM-yk, endoplasmic reticulum ER-gk (Nelson et al., 2007) or stained with 2 µg mL-1 DAPI (D1306; Invitrogen) to stain nuclei ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 × 107 AsiSI-ER-U2OS cells were double crosslinked with 1.5 mM EGS (ethylene glycol bis (succinimidyl succinate)) (ThermoFisher #21565) for 20 min at room temperature followed by 1% Formaldehyde (Merck ...
-
bioRxiv - Bioengineering 2020Quote: A cell line derived from Expi293 cells expressing an ER-localized biotin ligase (BirA) gene was grown in Expi293 media (Thermofisher Scientific) supplemented with 100 µM biotin (GoldBio ...
-
bioRxiv - Molecular Biology 2022Quote: The ER-HoxA9 cell line was cultured at 37 °C in a 5 % CO2 atmosphere using RPMI-1640 media (Life Technologies), supplemented with 10 % fetal bovine serum (Gemini Bio) ...
-
bioRxiv - Immunology 2022Quote: ... the DNA fragments encoding the spikes of the sarbecoviruses without the ER retrieval signal were codon-optimized and synthesized at GeneArt (Life Technologies). The spike encoding genes of Pang17 (residues 1-1249 ...
-
bioRxiv - Immunology 2022Quote: ... the DNA fragments encoding the spikes of MERS-CoV and sarbecoviruses without the ER retrieval signal were codon-optimized and synthesized at GeneArt (Life Technologies). The spike encoding genes of Pang17 (residues 1-1249 ...
-
bioRxiv - Immunology 2023Quote: ER and cytosol fractions from rPECs were prepared using the Mem-PER Plus Membrane Protein Extraction Kit (89842, Thermo Fisher Scientific). Briefly ...
-
bioRxiv - Genetics 2023Quote: ... M9 buffer was added and embryos were resuspended and transferred onto poly-lysine-coated slides (Thermo Scientific, cat#ER-308B-CE24) and sealed with a coverslip.
-
bioRxiv - Bioengineering 2023Quote: ... The ligand containing medium was then gently washed with fresh medium and replaced with 500 µL of fresh medium containing 5 µM ER Tracker Blue-White DPX (Thermo Fisher) and 1:1000 Cell-Mask Deep Red (Thermo Fisher) ...
-
bioRxiv - Microbiology 2023Quote: ... cells washed with PBS once and incubated for 30 minutes at 26°C with 1 μM live ER-tracker red dye (Molecular Probes) diluted in the appropriate Aag2 cell culture medium ...
-
bioRxiv - Cancer Biology 2023Quote: ... drug resistant (HCC827 ER, HCC827 LR) and drug removed lines (HCC827 ERDR) were cultured in RPMI-1640 (ATCC modification) (Gibco, A1049101) with 10% FBS and 1% PSQ ...
-
bioRxiv - Molecular Biology 2024Quote: ... U2OS and AsiSI-ER U2OS (gift from Legube’s Lab) cells were maintained under exponential growth in high-glucose DMEM medium (Life Technologies #31966047) supplemented with 10% (vol/vol ...
-
bioRxiv - Cell Biology 2020Quote: ... and 1 μg of plasmid encoding ER-targeted ESCargo(FTV) per dish using 2 μL of Lipofectamine 2000 reagent (Thermo Fisher; 11668019). Neurons were incubated for 48 h post-transfection and then imaged using a Leica SP5 confocal microscope as described above ...
-
bioRxiv - Molecular Biology 2020Quote: ... The K562-CEBPA-ER line was grown in 12 well plate in phenol red–free RPMI 1640 (ThermoFisher Scientific, Cat. No. 11835030), supplemented with 10% Charcoal stripped FBS (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ER-Hoxb8 cells were generated as described earlier (G. G. Wang et al., 2006) and grown in RPMI medium (Thermo Fisher Scientific) supplemented with 10% FBS (Biowest) ...
-
bioRxiv - Biophysics 2020Quote: ... The cell permeable organelle tracker Mito-Tracker Green FM (#M7514) and ER-Tracker Red (#E34250) and PM tracker CellMaskOrange (Thermo Fisher Scientifc) were used according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transfected with cDNAs encoding ER-eMagA (bait) and eMagB-TagRFP-T (prey) at a 2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
-
bioRxiv - Immunology 2022Quote: ... GMPs with the ER-HoxB8 construct successfully integrated were selected for with 2 mg/ml geneticin (G418; Thermo Fisher Scientific, Waltham, MA). Single-cell clones were sorted into a 96-well U-bottom plate via a BD FACSAria flow cytometer (BD Biosciences ...
-
bioRxiv - Cell Biology 2023Quote: The sequence encoding ER-NFAST was obtained by enzymatic digestion (HindIII + XhoI) of the above described ER-NFAST plasmid and ligated into pcDNA™FRT⁄TO vector (ThermoFisher Scientific).
-
bioRxiv - Molecular Biology 2021Quote: Oligo siRNAs were purchased from Dharmacon (siGENOME SMART pool) (Table S5) and were transfected into ER-Src cells using Lipofectamine RNAiMAX (Thermo Fisher Scientific, 13778050). 24 hours after transfection ...
-
bioRxiv - Neuroscience 2021Quote: Synaptosome and ER/Golgi fractions isolated from ZQ175 mouse brain by sucrose fractionation were dialyzed using Slide-A-Lyzer Dialysis Cassette (Thermo Fisher Scientific, 66203). The dialyzed mouse brain fractions isolated by sucrose fractionation were incubated with 0.01 μg/mL of Proteinase K at 37°C for 1 hr and heat inactivated at 75°C for 10 min ...
-
bioRxiv - Developmental Biology 2022Quote: ... containing 5mg/ml BSA (KSOM-BSA) then incubated in 500ul of KSOM-BSA containing 1µM ER Tracker-green (Life Technologies Corp. Eugene, OR) for 30 minutes at 37°C and 5% CO2 in a humidified incubator ...
-
bioRxiv - Cell Biology 2022Quote: ... at 2 mg/ml in PBS for 10 min or with Laurdan (see above) ± ER-Tracker™ Red (BODIPY™ TR Glibenclamide) (Thermo Scientific) at 0.5 μM ...
-
bioRxiv - Cell Biology 2024Quote: ... optionally followed by co-labeling with Cholesterol-Star488/ -StarOrange (Abberior 0206, 40 nM) for 10 min or ER-Tracker Red (Invitrogen E34250, 1 µM) for 30 min in cell culture medium at 37°C ...
-
bioRxiv - Biophysics 2023Quote: The freshly explanted tibiae were placed in a Petri dish containing ER TrackerTM Red (BODYPITM TR Glibenclamide) for cell imaging (Invitrogen, Eugene, Oregon, USA) in PBS medium) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and subsequently for 16 hours at 4°C in blocking solution containing primary antibodies (anti-A2AR mouse antibody, sc-32261 Santa Cruz Biotechnology, at 1:100 and anti-CD31 rat monoclonal antibody, ER-MP12 Thermo Scientific, 1:20). For detection of A2AR and CD31 ...
-
bioRxiv - Genetics 2022Quote: ... Exponentially growing cells were washed in fresh supplemented EMM media and a 2 μl drop was deposited on the well of a microscope slide (Thermo Scientific, ER-201B-CE24) covered with a layer of 1.4% agarose in filtered EMM ...
-
bioRxiv - Molecular Biology 2021Quote: MCF-10A-ER-Src cells (Iliopoulos et al., 2009; Iliopoulos et al., 2010) were grown in DMEM/F12 without phenol red (Thermo Fisher Scientific, 11039-047) + 5% charcoal stripped FBS (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... Each pooled seagrass sample was homogenized in a BashingBead Lysis Tube containing 2 mm ceramic beads and 800 μL RNA lysis bu?er (provided in the kit) for 5 minutes at maximum speed using a FisherbrandTM Bead Mill 4 Homogenizer (Fisher Scientific, Waltham, MA, USA). Tissue homogenates were centrifuged at maximum speed (21,130 g ...
-
bioRxiv - Cell Biology 2020Quote: ... For dye labeling the growth medium was replaced with PBS containing the organelle specific dyes MitoTracker® Red FM (25 nM) or ER-Tracker™ Red (BODIPY® TR Glibenclamide) (1μM) (Life Technologies GmbH, Frankfurt). After incubation with MitoTracker® Red FM for 5 min or with ER-Tracker™ Red (BODIPY® TR Glibenclamide ...
-
bioRxiv - Microbiology 2024Quote: ... 20 μl of purified sporozoites were allowed to adhere to the wells of a glass slide (Thermo Scientific ER-308B-CE24, 10 wells 6.7 mm glass) and fixed for 10 minutes with 4% PFA ...