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Citations for Thermo Fisher :
101 - 150 of 178 citations for docosyl dihydrogen citrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Cells were then sonicated for 10 sec at 20% power and 0.5 mL of citrate buffer containing 2 μM of SYTOX green (Invitrogen) was added to the suspension ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were incubated in a 10 mM sodium citrate buffer with 0.05 % Tween 20 (pH of 6) (Fisher Scientific) for 15 minutes at 99□ prior to fixation ...
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bioRxiv - Neuroscience 2020Quote: ... where sections were heated to 100°C for 20 min in citrate buffer (TA-250-PM1X; Thermo Scientific, UK). After cooling in dH2O ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were contrasted with 0.4% lead citrate for 1 min and imaged in a Tecnai T12 transmission electron microscope (ThermoFisher), operated at 100 kV and equipped with an axial 2k CCD camera (TVIPS).
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bioRxiv - Immunology 2022Quote: Blood was collected in collection tubes containing anticoagulant acid citrate dextrose (ADC) Solution B (Fisher Scientific, Hampton, NH, USA). Ficoll (Stemcell Technologies ...
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bioRxiv - Genomics 2021Quote: ... Cell aggregates were then dissociated by sonication and 40 μL of cell suspension were incubated with 170 μL of 50 mM sodium citrate buffer containing 0.5 μM Sytox Green (Invitrogen). Data were acquired on a MASCQuant Analyzer (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... Heat-induced antigen retrieval was performed by incubating slides in a solution of 10 mM sodium citrate pH 6 (MilliporeSigma; SX0445-20) with 0.05% (v/v) Tween20 (ThermoFisher) at 98C for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and re-fixed for 10 minutes before being briefly rinsed with wash buffer (2x saline-sodium citrate [SSC, Invitrogen, AM9770] ...
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bioRxiv - Cell Biology 2021Quote: ... was modified by adding the following reagents to the stated final concentrations: 1mM trisodium citrate dihydrate (ThermoFisher Scientific, BP327), 91.2mM sodium chloride (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... Ultrathin sections were stained with uranyl acetate and lead citrate and analysed with a Tecnai Spirit (Thermo Fisher Scientific) operated at 120 kV.
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed gradually into 2X SSC buffer (0.3 M sodium chloride, 30 mM sodium citrate or AM9763, Invitrogen), and then gradually from SSC to PBT ...
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bioRxiv - Developmental Biology 2023Quote: ... Grids were stained with lead citrate (Merk) and examined with a Tecnai T12 transmission electron microscope (Thermo Fisher Scientific). Digital electron micrographs were acquired with a bottom-mounted TVIPS TemCam-XF416 4k × 4k CMOS camera.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were pelleted at 3,000 g, aspirated, resuspended in SYTOX buffer (500 µL 50mM sodium citrate [pH 7], 1 µM SYTOX green (Invitrogen)) ...
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bioRxiv - Biophysics 2023Quote: ... Fresh human blood was drawn into a citrated tube (3.8% sodium citrate, citrate:blood = 1:9) and mixed with MitoTracker Deep Red (Invitrogen, USA). Citrate chelates extracellular calcium ...
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bioRxiv - Biochemistry 2023Quote: ... 30 μl of each sample were mixed with 170 μl of 50 mM Na-citrate and 0.5 μM Sytox Green (S7020, ThermoFisher). Prior to the FACS analysis ...
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bioRxiv - Biophysics 2023Quote: ... containing 10,000 units/mL of penicillin 10,000μg/mL of streptomycin in a 10 mM citrate buffer; Fisher Scientific Ltd). Cells were kept at 37°C and 5% CO2 as standard and were used 2 days after passage.
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bioRxiv - Physiology 2022Quote: Maximal enzyme activity of citrate synthase was determined from muscle homogenate using fluorometry (Fluoroscan Ascent, Thermo Fisher Scientific, Waltham, USA) at 25°C as previously described (Lowry ...
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bioRxiv - Biophysics 2021Quote: Cells were magnetically labelled thanks to an incubation of 2h with a solution of iron oxide nanoparticles at [Fe] = 4 mM and supplemented with 5 mM citrate in RPMI medium (Gibco). The viability and the proliferation of cells after magnetic labelling were assessed by Alamar Blue showing no impact of the magnetic labelling right after the labelling or after one day (Supplementary Figure 1).
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bioRxiv - Bioengineering 2019Quote: Paraffin-embedded aorta sections mounted slides were subjected to heat-induced antigen epitope retrieval with citrate buffer (Thermo Scientific, MA). The slides were incubated overnight at 4°C with primary antibodies for anti -MMP-9 (Rabbit anti-Mouse ...
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bioRxiv - Genetics 2022Quote: ... at -20°C for 15 min followed by washing three times in 2X saline sodium citrate (SSC) buffer with 0.1% Tween-20 (2xSSCT; made from 20xSSC, ThermoFisher, AM9763) at room temperature for 30 min each.
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bioRxiv - Genetics 2022Quote: ... DNA was stained by addition of 500 µL of Sodium Citrate (50 mM) containing 0.2 µL SYTOX-Green (Life Technologies). Cells were disrupted by brief sonication ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stored at 4°C for at least overnight then wash several times with 2X saline sodium citrate (SSC; Ambion). Cell were equilibrated in hybridization buffer (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and adding citrate ethanol before optical density (OD) was measured at 540 nm (Labsystems Multiskan Ascent Reader, Thermo Fisher Scientific). OD was directly proportional to viable cells ...
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bioRxiv - Plant Biology 2021Quote: ... protoplasts were resuspended in lysis buffer (45mM magnesium chloride, 30mM sodium citrate, 20mM MOPS, 0.5% triton, 2% 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), pH 7 ...
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bioRxiv - Microbiology 2022Quote: ... was generated using an established low-pH model.7 Mid-log Mtb-lux was washed with PBS (1X) and transferred into PCB media: 1X phosphate-citrate buffer (PCB, composed of 0.2 M sodium phosphate (Fisher Scientific) with 0.1 M citric acid (Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... were stained with uranyl acetate and Reynolds lead citrate and examined at 100 kV with a PHILIPS EM208S TEM (FEI - ThermoFisher) equipped with the Megaview III SIS camera (Olympus).
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bioRxiv - Genetics 2022Quote: ... Fixed worms were washed 3 min in 1mL wash solution (10% deionized formamide and 2x Saline Sodium Citrate buffer (Ambion) in RNase-free water) ...
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bioRxiv - Bioengineering 2023Quote: ... Antigen retrieval was performed by heating the slides in 10 mM sodium citrate buffer pH 6.0 (Thermo Fisher, S279-3) for 4 min in a microwave and left to cool down for 10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: Sample inactivation was performed by mixing rigorously 175 µl sodium citrate-treated blood with TRIzol LS Reagent (ThermoFisher Scientific, 10296028), or by mixing 70 µl swab elute or tissue homogenates with 630 µl TriPure Isolation Reagent (Sigma–Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... antigen retrieval was performed using citrate (pH 6.0) or Tris-EDTA buffer as previously described prior to incubation with BlockAid Blocking Solution (Thermo Fisher). For IHC ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were boiled in sodium citrate buffer (pH 6.0) for 15 min and incubated with the following antibodies for 1h: anti-CD8 (4SM15, Thermo Scientific), anti-NCR1 (EPR23097-35 ...
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bioRxiv - Biochemistry 2024Quote: ... and 17 µL of 1x RNA Sequencing Buffer (20 mM sodium citrate pH 5, 1 mM EDTA, 7 M urea) (Ambion). The mixture was split across three tubes (9/8/8 µL) ...
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bioRxiv - Immunology 2024Quote: PBMCs were isolated from whole blood samples collected in 8.5mL BD Vacutainer glass collection tubes with acid citrate dextrose (ACD) used as an anticoagulant (Fisher Scientific). Two tubes were collected from each participant ...
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bioRxiv - Cell Biology 2019Quote: ... from the imaged animals were cut and stained with uranyl acetate/lead citrate (Agar Scientific, Stansted, UK) and imaged in a Tecnai T12 transmission electron microscope (Thermo Fisher) operating at 100 KV equipped with a digital camera (Veleta) ...
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bioRxiv - Bioengineering 2021Quote: ... A 1X stock solution of saline-sodium citrate (SSC) buffer was prepared by dilution of SSC stock solution (20X, pH 7.4, Thermo Fisher Scientific) with nuclease-free water ...
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bioRxiv - Bioengineering 2020Quote: ... and subjected to heat-induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, Fisher Scientific International, Inc., Pittsburgh, MA) at >80°C ...
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bioRxiv - Microbiology 2021Quote: ... before staining with lead citrate and examined using a transmission electron microscope (Tecnai Spirit G12; Thermo Fisher Scientific/FEI, Eindhoven, Netherlands) at 120 kV ...
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bioRxiv - Microbiology 2020Quote: ... and beads were washed 5x 2 minutes in 1 mL of ChIRP Wash Buffer (2x NaCl-Sodium Citrate (SSC, ThermoFisher Scientific), 0.5% SDS ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were recovered by centrifugation and resuspended at a concentration of 106 cells/mL in 50 mM sodium citrate with 2 μM Sytox Green (Life Technologies). After 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 20 µL of this cell suspension were incubated with 200 µL of 50 mM Na-Citrate buffer containing 4 µg/mL Propidium Iodide (Fisher scientific). Data were acquired and analyzed using a Novocyte Express (Novocyte) ...
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bioRxiv - Biophysics 2022Quote: ... Cells were washed with 2x saline-sodium citrate (SSC) two times followed by a final wash with FISH wash solution (10% formamide (Ambion, #AM9342) in 2x SSC) ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by three more washes of Buffer B and one wash with 10 % formamide in 2x saline-sodium citrate (SSC) buffer (ThermoFisher AM9770). Cells were resuspended in the formamide/SSC solution and split evenly into two replicate samples for hybridization ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed with 2× saline sodium citrate (SSC) buffer for 5⍰min and blocked with UltraPure Salmon Sperm DNA (Thermo Fisher) in QuikHyb Hybridization Solution (Agilent Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were processed through de-waxing and antigen retrieval in citrate buffer at pH 6.0 using a heat-induced epitope retrieval PT module (Thermo Fisher Scientific) before proceeding with immunostaining ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then pelleted and resuspended cells in sodium citrate and added 30 µL of 50 µM SYTOX green nucleic acid stain (Invitrogen S7020). Finally ...
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bioRxiv - Genetics 2023Quote: ... Cells were then washed and incubated for 30 minutes with 1 mL of 50 mM Sodium citrate pH 7.0 with 16 µg of Propidium iodide (Fisher Scientific, 11425392). Flow cytometry profiles were obtained on a MACSQuant machine and analyzed using Flowing Software 2.5.1.
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bioRxiv - Developmental Biology 2023Quote: ... Antigen unmasking was performed by microwaving the slides for 2 × 10 min in citrate buffer solution (pH 6) (Thermo Fisher Scientific). Sections were then blocked for 1 hour at room temperature with 10% normal donkey serum (Chemicon International Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... in 25 mM sodium citrate pH 5.0 buffer containing 7 M urea and 10.5 mM EDTA with 1 U RNase T1 (ThermoFisher Scientific, Waltham, MA) at 55°C for 5 min ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... Antigens were retrieved by incubating the slides in Citrate Buffer pH 6.0 at 95°C for 25min using a PT module (ThermoFisher Scientific, Paisley, UK). Endogenous peroxidase was then blocked by incubating slides in 3% H2O2 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed in formamide/SSC solution (formamide: Invitrogen, ThermoFisher Scientific, USA; 20x SSC, saline-sodium citrate buffer: Gibco, ThermoFisher Scientific, USA) at 65°C and in MAB solution (maleic acid buffer ...