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Citations for Thermo Fisher :
101 - 150 of 193 citations for Zwitterionic Detergent MD3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The hTau in PBS or detergent treated media was quantified using total human tau ELISA (Invitrogen).
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bioRxiv - Genetics 2024Quote: ... Total proteins were quantified using either the Pierce™ Detergent Compatible Bradford Assay Kit (ThermoFisher Scientific) or DC Protein Assay (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was determined using a Pierce 660 nm kit with ionic detergent compatibility reagent (Thermo Fisher Scientific Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was determined using Pierce 660 nm kit with ionic detergent compatibility reagent (Thermo Fisher Scientific), and 12 μg of each sample was loaded on a 12.5% SDS-PAGE gel and run ~1 cm into the resolving gel ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration in the supernatant was estimated using the Pierce Detergent Compatible Bradford Assay Kit (Thermofisher, 23246). For each Western Blot ...
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bioRxiv - Biochemistry 2023Quote: ... The IM-containing supernatant was applied to a 4 mL Pierce detergent removal spin column (ThermoFisher Scientific) for removal of Triton X-100 and frozen at -20°C ...
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bioRxiv - Neuroscience 2023Quote: ... Quantities of cortical proteins were determined by Pierce™ Detergent Compatible Bradford Assay Kit (Thermo Scientific™).
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bioRxiv - Biophysics 2023Quote: ... The sample was purified using High Protein and Peptide Recovery Detergent Removal Spin Column (Thermo Fisher Scientific) and Pierce peptide desalting spin columns (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Eluates were load balanced (20 µg) and surfactant depleted with Pierce Detergent Removal Spin Columns (ThermoFisher Scientific). Samples were reduced with TCEP (10 mM) ...
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bioRxiv - Immunology 2024Quote: ... Lysates were quantified using the 660 nm Protein Assay with the Ionic Compatibility Detergent Reagent (ThermoFisher, 22663) and normalized to the same concentration with 1x RIPA buffer.
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bioRxiv - Immunology 2024Quote: ... Total protein concentration was measured using a standard Bradford assay (Pierce Detergent Compatible Bradford Assay Kit, ThermoFisher), and 150ugs of total protein was incubated on membranes pre-spotted with capture antibodies for 59 immune-related receptors ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein concentration of the lysates was measured using Pierce Detergent Compatible Bradford Assay Reagent (Thermo Fisher Scientific) and adjusted in buffer E ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific). Desalted peptides were solubilized in 0.1% formic acid and 3% acetonitrile ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse brains were lysed in PBS without detergent and supplemented with EDTA-free protease inhibitor cocktail (Thermo Fisher). The post-nuclear supernatant was incubated with anti-myc magnetic beads (with covalently cross-linked clone 9E10 mAb ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested samples were purified using High Protein and Peptide Recovery Detergent Removal Spin Column (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-NavSp1 was applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Peptide solutions were separated from magnetic beads and further processed with HiPPR detergent removal spin columns (Thermo Scientific) and desalted by reverse phase C18 ziptip (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were cleaned-up using Pierce® Detergent removal spin columns (Thermo Fisher Scientific, cat. No. 87777) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Detergent removal was performed with 50 μL of each fraction using 0.1 mL HIPPR resin (Thermo Scientific, 88305) followed by TCA precipitation of the samples ...
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bioRxiv - Bioengineering 2024Quote: Protein quantification was performed using the Pierce Detergent Compatible Bradford Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) following manufacturer’s specifications ...
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bioRxiv - Biochemistry 2024Quote: ... The TMT-set was further purified using High Protein and Peptide Recovery Detergent Removal Spin Column (88305, ThermoFisher) and Pierce peptide desalting spin columns (89852 ...
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bioRxiv - Neuroscience 2023Quote: ... double immunofluorescence labeling with detergent was performed on 50-µm sections with the anti-pTau mAb AT8 (ThermoFisher) plus the anti-mouse IgG H + L secondary antibody conjugated with Alexa Fluor 488 (ThermoFisher) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein concentrations were measured using the Bradford assay (Pierce™ Detergent Compatible Bradford Assay Kit, Thermo Scientific, USA), and equal amount of proteins were loaded on a SDS-PAGE gel for western blot.
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bioRxiv - Genomics 2024Quote: ... Protein content in the supernatant was measured using Pierce Detergent Compatible Bradford Assay kit (ThermoFisher Scientific; ref. 23246) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 2020) using the manufacturers detergent for 5 min permeabilization and all probe sets (incubation time 3h, Thermo Fisher ViewRNA probe sets ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined using PierceTM 660nm Protein Assay with the ionic detergent compatibility reagent (Thermo Fisher Scientific). Sarkosyl-soluble aliquots of 100 μg and sarkosyl-insoluble aliquots of 40 μg were stored at - 80°C.
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bioRxiv - Biophysics 2020Quote: ... Protein was added to the solubilized lipid-detergent mixture and placed into 10,000 MWCO dialysis cassettes (Thermo Fisher Scientific), and then the samples were then dialyzed in the dark at 4 °C against 4 L of DB ...
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bioRxiv - Microbiology 2022Quote: ... The pellet containing the membranes was resuspended in 20 mM phosphate buffer pH 7.4 with 0,3% n-octyl-poly-oxyethylene (OPOE) detergent (Affymetrix, UK) and incubated at 20°C for 2 h under agitation to separate inner (IM ...
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bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-tagged proteins were applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted beads were discarded on a magnet and eluate was purified on HiPPR Detergent Removal Resin (Thermo Scientific, 88305). Purified proteins were eluted in PBS ...
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bioRxiv - Microbiology 2020Quote: ... at day 0 the animals were subcutaneously administered either approximately 10,000,000 fffus of detergent-inactivated UHV or 0.1 mg of recombinant UHV NP emulsified in Freund’s incomplete adjuvant (ThermoFisher Scientific), the total volume per individual was 125 μl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and peptides were purified using HiPPR detergent removal kit and Pierce peptide desalting spin columns (both Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 10x serial dilutions were made in 0.1% formic acid 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) in LCMS grade water ...
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bioRxiv - Plant Biology 2024Quote: Frozen chloroplast aliquots were thawed on ice and NP-40 Surfact-Amps™ Detergent Solution (ThermoFisher, cat. no 13444269) was added to 0.8 % ...
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bioRxiv - Biochemistry 2022Quote: Protein samples were quantified using Pierce™ 660 Protein Assay Kit (plus Ionic Detergent Compatibility Reagent, IDCR; Thermo Scientific). Then ...
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bioRxiv - Physiology 2023Quote: ... protein concentrations in detergent-soluble cell lysates were determined using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific), and proteins were subsequently analyzed by western blot ...
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bioRxiv - Biophysics 2023Quote: ... Protein was added to the solubilized lipid-detergent mixture and placed into 3,500 MWCO dialysis cassettes (Thermo Scientific #66330), and then the samples were then dialyzed in the dark at room temperature in 1000x sample volume with 4 buffer changes every 4–12 hours ...
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bioRxiv - Microbiology 2024Quote: ... All samples were then quantified using the Pierce 660nm assay with the ionic detergent compatibility reagent (Thermo Fisher Scientific); and standardized to 50 µg ...
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bioRxiv - Genomics 2024Quote: ... and protein concentrations were quantified in triplicate using the Pierce detergent Compatible Bradford Assay kit (Thermo Scientific, Cat#23246), with 150 µL of reagent and 5 µL of sample per well ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were pelleted and 50μg of protein was quantified by ionic detergent compatible Pierce 660nm Protein Assay Reagent (Thermo Fisher) in 8M Urea ...
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bioRxiv - Biophysics 2021Quote: ... and detergents and concentrate the proteins in the column for the efficient digestion then digested with trypsin (Thermo Fisher Scientific) in 1:10 (w/w ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of detergent-soluble lysates were resolved using the NuPAGE Novex Midi Gel system on 4–12% Bis–Tris gels (Invitrogen). Proteins were transferred to PVDF membranes (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: Protein Digestion and Tandem Mass Tag (TMT) labeling: The Pierce detergent compatible Bradford assay kit from Thermo Fisher (Winnipeg, MB) was used to determine the protein concentration ...
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bioRxiv - Neuroscience 2023Quote: ... An aliquot of Glucose Uptake Glo assay lysates was analyzed for total protein content using the Pierce 660 nm protein assay according to the manufacturer’s instructions and with Ionic Detergent Compatibility Reagent (Thermo Scientific). Luminescence values were then normalized to total protein.
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration of the samples was determined using a Pierce Detergent Compatible Bradford assay kit according the manufacturer’s instructions (Thermofisher, 23246). Aβ40 and Aβ42 measurements of the samples were done according to the manufacturers protocols using Mesoscale Discovery V-plex Plus Aβ42 (4G8 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mL fractions were collected and the fractions containing protein were determined using Detergent Compatible Bradford assay (Thermo Scientific 23246). Fractions were dialyzed against 1 L of Tn5 Size Exclusion Chromatography (SEC ...
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bioRxiv - Immunology 2024Quote: ... They were then removed from surrounding fat by dissection and mild detergent wash (<1 sec) in 0.01% digitonin solution (Thermo Scientific) and embedded in 6% w/v low melting point agarose (Lonza ...
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bioRxiv - Biochemistry 2024Quote: ... was serial diluted using a solution containing 0.1% FA in LCMS grade water (Pierce 85170) and 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902). A total of 10 microliters of each dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Total protein concentration for the detergent soluble fractions were measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and Biotek Synergy HT plate reader according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations were quantified using the Pierce 660 nm Protein Assay Reagent supplemented with Ionic Detergent Compatibility Reagent according to the 96-well microplate procedure of the manufacturer’s protocol assay (Thermo Fisher Scientific).