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Citations for Thermo Fisher :
101 - 150 of 225 citations for Sitagliptin Impurity 43 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... the plates were sealed with MicroAmp Optical Adhesive Film (Thermofisher, cat#: 43-119-71) and allowed to incubate at RT in the dark for at least 15min to rehydrate primer ...
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bioRxiv - Developmental Biology 2024Quote: ... the PBS was replaced with pre-warmed (43°C) 40% wHyb (2x SSC, Invitrogen; 40% deionized formamide ...
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bioRxiv - Bioengineering 2023Quote: GBM-43 and GBM-6 (Mayo Clinic) were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2024Quote: ... larvae at 5 dpf were incubated in 3 µM FM 1-43 (Thermofisher, T3163) in E3 media for 35 s ...
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bioRxiv - Neuroscience 2021Quote: ... spanning exons 40-43) was cloned from mouse brain cDNA into pCRII-TOPO (Thermo Fisher). The plasmid was linearized and subjected to in vitro transcription with digoxigenin labeling (DIG RNA labeling kit ...
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bioRxiv - Biochemistry 2021Quote: TDP-43-MBP-His6 variants were labeled with Alexa Fluor 488 C5 maleimide (Thermo Fisher) at a low (~0.01-0.05 ...
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bioRxiv - Cancer Biology 2021Quote: ... the enzyme activity was analyzed by Amplex Red hydrogen peroxidase assay kit (43) (Thermo Scientific). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with fixation/permeabilization buffer (Invitrogen 00-5123-43 and 00-5223-56) for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Stained cells were fixed using eBioscience Fixation/Permeabilization Concentration (Invitrogen, catalog number 00-5123-43). The data were acquired on the Cytek Aurora spectral flow cytometer and analyzed using the Flow Jo software v10.9.0 ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 100µL of 1:4 Fixation/Permeabilization concentrate (Invitrogen 00-5123-43) in Fixation/Permeabilization diluent (Invitrogen 00-5223-56 ...
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bioRxiv - Neuroscience 2024Quote: The FM™ 1-43 (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide) (Invitrogen) membrane probe was used to identify actively firing neurons and to investigate mechanisms of activity-dependent vesicle cycling ...
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bioRxiv - Neuroscience 2021Quote: ... TDP-43 (amino acids 1-414) and NPM1 were PCR amplified from HEK293FT (Thermo Fisher Scientific) cDNA and subsequently cloned into the FhSynW backbone (human synapsin promoter ...
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bioRxiv - Microbiology 2020Quote: ... ex vivo Ena were vacuum dried at 43 °C using Savant DNA120 Speedvac Concentrator (Thermo scientific) run for 2 hours at a speed of 2k RPM.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... CHO cells stably expressing TRPV4 (43) were cultured in Ham’s Nutrient Mixture F12 (Thermo Fisher Scientific), supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... as described previously [43]) were maintained in Glasgow Minimum Essential Medium (GMEM, Thermo Fisher Scientific, #221000093) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Real time qPCR was done using Fast SYBR Green Master Mix (Applied Biosystems #43-856-12) and ran on QuantStudio six Flex real-time PCR system (Applied Biosystems # 4485691) ...
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bioRxiv - Neuroscience 2022Quote: ... NGN2-induced glutamatergic neurons [43] were generated and co-cultured with human primary astrocytes (ThermoFisher #N7805200) for all experiments [44].
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bioRxiv - Neuroscience 2024Quote: ... for phospho-TDP-43 and goat anti-mouse Alexa Fluor® 568 (1:500 A11004, Invitrogen) for p62 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was prepared using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems 43-688-14), then amplified with SYBR green (Bio-Rad 1708882 ...
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bioRxiv - Developmental Biology 2024Quote: 3 dpf larvae were incubated in 3 µM FM™ 1-43 Dye (Thermofisher catalog # T3163) for 2-4 minutes ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted as described previously (43) and treated with TURBO DNA-free™ Kit (Life Technologies) to remove contaminating genomic DNA ...
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bioRxiv - Neuroscience 2020Quote: Functional nerve terminals (boutons) were stained by labeling recycling synaptic vesicles with FM1-43 (T35356; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... [43] using a real-time PCR system (Step One Plus Real-Time PCR System, Applied Biosystems, Singapore). The reaction mixture (20 μL ...
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bioRxiv - Neuroscience 2023Quote: ... The pUAST-FUS or pUAST-TDP-43 vectors were generated using Gateway recombination reactions (Thermo Fisher Scientific). The FUS RRM mutant construct (pUAST-FUS-RRMmut) ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection solution was prepared by mixing 43 µl RNAi MAX with 500 µl Opti-MEMTM (Thermo Scientific) and separately 14.5 µg CRISPR/Cas9 plasmid containing the cloned protospacer with 500 µl Opti-MEMTM (Thermo Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... Bulk GBM cell lines (GBM-43, GBM-6) were harvested using 0.25% Trypsin-EDTA (Thermo Fisher Scientific) and passaged less than 30 times.
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bioRxiv - Microbiology 2022Quote: ... Env accession number JQ867464)43 was cloned into a modified pMT/BiP insect cell expression plasmid (Invitrogen) designated pT350 ...
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bioRxiv - Immunology 2021Quote: ... then reverse transcribed into cDNA using a high-capacity cDNA reverse transcription kit (Thermo Fisher, 43-688-13). RNA levels were quantified using the IDT Prime Time Gene Expression Master Mix ...
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bioRxiv - Neuroscience 2020Quote: ... Melanopsin C268 was constructed using Gibson assembly (43) of synthetically-synthesized Strings DNA fragments (ThermoFisher Scientific, Waltham, MA) to generate mutagenesis of residues C95 ...
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bioRxiv - Cell Biology 2022Quote: ... nerves were cut and subsequently incubated for 10 min in HL3 with 4 µM FM 1-43 (Invitrogen), 1.5 mM CaCl2 and 90 mM KCl ...
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bioRxiv - Microbiology 2020Quote: ... Signal development was accomplished using a 1:200 dilution of streptavidin-horseradish peroxidase (Invitrogen; Cat. No. 43-4323) for 30 minutes followed by incubation with Nova Red chromagen solution (Vector ...
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bioRxiv - Microbiology 2021Quote: ... HeLa cells were maintained as previously described (42, 43) in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS (Invitrogen) and grown at 37°C in a humidified atmosphere of 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Intracellular staining was done using the fixation/permeabilization buffer (#00-5123-43 and #00-8333, Thermo Fisher Scientific) and anti-Ki67-BV786 (#563756m BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... RNA was converted into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems #43-688-14) using 2 µg RNA per 20 µl reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... the PBS was replaced with pre-warmed (43°C) 40% wHyb (2x SSC, Invitrogen; 40% deionized formamide, Invitrogen; 1% Tween-20 ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA conversion was performed using the high-capacity cDNA reverse transcription kit (Thermo Fisher, Cat. #43-668-13) according to manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... and the cells were maintained as previously described.43 Cells were cultured in T-75 flasks (Fisher Scientific) and incubated at 37 °C and 5% CO2 and tested negative for mycoplasma quarterly using a PCR-based assay.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... lhfpl5bvo35homozygotes were identified from their hetero-and homozygous wild type siblings by lack of FM 1-43 (ThermoFisher) labeling of the lateral line hair cells.
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bioRxiv - Evolutionary Biology 2024Quote: ... FM1-43 membrane stain (1 μM, exc. 488 nm em. 580–620 nm, Invitrogen, incubation time: 24 h), and membrane impermeable Calcein (0.7 mg/10 mL ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription PCR was performed using the High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific, 43-688-14). Quantitative PCR is performed on the QuantStudio 3 using SYBR Green (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: HA tagged human Q331K mutant TDP-43 was cloned in the pcDNA5/FRT/TO plasmid (Invitrogen™ V652020) and sequence verified ...
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bioRxiv - Cell Biology 2024Quote: ... cells were permeabilized and fixed with the eBioScience Fix/Perm kit (Invitrogen, #00-5123-43 #00-5223-56) according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 ng of RNA was reverse transcribed using the TaqMan reverse transcription kit (ThermoFisher, cat # 43-665-96).
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bioRxiv - Cell Biology 2022Quote: ... images of live cell morphology were captured using the epifluorescence mode and with FM™ 4-43 (Invitrogen, T3166) added right before imaging.
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected and stimulated with a HL-3 solution of 90 mM K+/0.5 mM Ca2+/4 μM fixable FM1-43 (Invitrogen) for 1 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and the samples were incubated with pre-warmed (43°C) 40% wHyb (2x SSC (Thermo Fisher Scientific, cat. #15557044), 40% deionized formamide (MilliporeSigma ...
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bioRxiv - Cell Biology 2021Quote: ... EB1-GFP [43] (gift from P. Draber, IMG ASCR, Prague, Czech Republic) were grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from the deposited blood using the PureLink Total RNA Blood Purification kit by Invitrogen (43). The volume of lysis buffer (L5 ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was synthesized using the Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific, 43-688-13) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was converted into cDNA using the High Capacity cDNA Reverse Transcriptase kit (Thermo Fisher Scientific #43-688-13) according to manufacturer’s instructions ...