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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Siglec 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
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bioRxiv - Immunology 2021Quote: ... was coated with 2μg/mL goat anti-human Fc antibody (Thermo Fisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... USA) and anti-human IgG Fc specific HRP conjugated secondary antibodies (ThermoFisher Scientific) were added ...
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bioRxiv - Immunology 2022Quote: ... medium supplemented with 10 ng/ml human GM-CSF and 20% FCS (Gibco). For the purposes of proliferation assays ...
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bioRxiv - Immunology 2023Quote: ... or HRP-conjugated rabbit anti-human IgG Fc antibodies (Invitrogen, 31423, 1:5,000) diluted in blocking buffer were added to the wells incubated with phages or recombinant proteins ...
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bioRxiv - Immunology 2020Quote: ... 1mM sodium pyruvate and 3.7 g/L NaHCO3 and supplemented with 15% FCS (Gibco, A3160801), 10 mM HEPES (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Monoclonal Antibody Siglec-F (CD170, 1:300, 14-1702-82, ThermoFisher Scientific). Quantitative score of Siglec10 positive cells in the human brains was the average counts of two brain sections in a power (2,000X ...
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bioRxiv - Immunology 2024Quote: ... BUV 737 rat anti-mouse Siglec F (Invitrogen Cat# 367-1702-82), Pe-Cy5 rat anti-mouse CD326(Ep-CAM ...
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bioRxiv - Cell Biology 2021Quote: ... 15 × 104 wildtype HEK293 cells or SMAP1 KO A2 cells were transfected with GFP-vWF with Lipofectamine LTX (Thermo Fisher) by reverse transfection ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were seeded at ∼10-15 % confluency 48 hours before a triple transfection with Lipofectamine 2000 (Thermo Fisher Scientific) as per the manufacturers protocol.
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK293) cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were then transfected into human embryonic kidney cells (HEK293-E, National Research Council, Canada) using 293Fectin (Thermofisher). Six days post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Immunology 2023Quote: ... After treating the cells for 15 minutes (min) with Fc Receptor Binding Inhibitor Polyclonal Antibody (ThermoFisher), they were stained for 30 min at 4°C in the dark and washed twice again ...
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bioRxiv - Cancer Biology 2024Quote: ... KELLY cells were cultured in RPMI medium with 10-15% fetal calf serum (FCS) (Life Technologies). Cells were maintained at 5% CO2 and at 37°C and were split at full confluency (at 5% CO2 and at 37°C ...
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bioRxiv - Immunology 2019Quote: ... and the ELISPOT plate was incubated with biotinylated goat anti-human IgG Fc (Invitrogen), followed by HRP-conjugated avidin (Vector Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated antibody specific for the Fc region of human IgG (Invitrogen) was used as secondary antibody to detect antibody binding in ELISA experiments.
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... nanovials were modified with biotinylated goat anti-human IgG Fc antibody (Thermo Fisher Scientific). Samples were resuspended in DMEM and seeded in a well plate ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated Abs specific for the Fc region of human IgG (Invitrogen) were used as secondary antibodies to detect antibody binding in ELISA experiments ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Protease susceptibility measurements were performed in biological replicates (n = 2 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Titrations were performed in biological replicates (cultures from independent colonies grown on separate days ...
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bioRxiv - Immunology 2020Quote: ... followed by addition to the Fc-chimeric human ACE2-coated MaxiSORP ELISA plate (Nunc) for an hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Human JAGGED1-Fc (Martin et al., 2023) was transfected into Expi293F cells (ThermoFisher, A14527) using FectroPro (Polyplus ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human Fc chip was regenerated to baseline using IgG Elution Buffer (Thermo Scientific) and the process was repeated for all humAbs in the panel ...
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bioRxiv - Immunology 2020Quote: ... cells were pre-treated with 20µg/ml Siglec-1 mAb (Invitrogen, HSn 7D2) for 30 minutes before HIV was added.
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bioRxiv - Biochemistry 2022Quote: HEK293 wild type (ATCC, #CRL-1573) and HEK293 Flp-in T-REx (ThermoFisher, #R78007) wild type cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 Flp-In T-REx cells (or HEK293 T-REx; Thermo Fisher Scientific, R78007) and HEK293 cells [American Type Culture Collection (ATCC) ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 4 × 106 HEK293 Flp-In T-Rex cells with EGFP integrants were seeded in 15-cm dishes (Thermo Fisher Scientific) and incubated overnight ...
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bioRxiv - Cancer Biology 2021Quote: HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... HEK293 Freestyle suspension cells (ThermoFisher), and immortalized human cord blood-derived mesenchymal stromal cells (cbMSCs ...
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bioRxiv - Neuroscience 2020Quote: HEK293-FT (RRID:CVCL_6911, Thermo Scientific) cells were grown in DMEM containing 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-FlpIn cells (ThermoFisher Scientific) were transfected with empty vector (negative control ...
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bioRxiv - Biochemistry 2022Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293-FT (Thermo Fisher Scientific) in DMEM with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293-F cells (ThermoFisher R79007) were transfected with the transfer plasmid and the packaging plasmids (Addgene plasmids #12263 and #8454 ...
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Human parental HEK293 cells (ATCC, CRL-1573) were cultured in DMEM supplemented with 10% FBS and antibiotics/antimycotics (Gibco).
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney (HEK293) and A549 cell lines were maintained in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Lithuania) containing 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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bioRxiv - Biophysics 2020Quote: ... cell medium was replaced by 1 ml Leibovitz’s L-15 Medium (c.c.pro) containing 10% FCS and 25 nM Hoechst 33342 nucleic acid stain (Invitrogen) and incubated for 1 h at 37 °C before imaging.
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bioRxiv - Immunology 2022Quote: ... After another round of Fc blocking on ice for 15 min with sera from mouse (Thermo Fisher) and rat (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... 1:500 dilution) (goat anti-human IgG Fc Alexa 488 conjugated, Thermo Fisher, Cat #: H10120) (goat anti-human IgG (H + L ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells stably transfected with human CXCR4 (HEK293- CXCR4) were used at passage 5 and were cultivated in DMEM medium (Gibco), supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: The template RNA for cDNA synthesis was isolated from human HEK293 cell culture with TRIzol reagent (Thermo Fisher Scientific, USA), or FFPE samples ...