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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and SB28 RRV-IRF8 (100% transduced) cells were plated at 1x105 cells (n=3 per time point) and counted (Thermofisher Countess 3)at 24 and 48 hours post-seeding ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2021Quote: ... cells of the first aliquot were treated with N*-(3-maleimidylpropionyl) biocytin (MPB, Thermo Scientific) at a final concentration of 107 μM for 20 min on ice ...
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bioRxiv - Plant Biology 2024Quote: ... rafflesiana n = 3) were determined colorimetrically using the Bradford protein assay kit (Thermo Scientific, USA) with bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... TIC-enriching 3-D cultures (3-D) were maintained in stem cell media: DMEM:F12 (+ L-glutamine, + 15 mM HEPES) (Gibco) supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Neuroscience 2023Quote: ... particle size = 3 μm, C18, L = 2 cm; analytical column: particle size = 2 μm, C18, L = 50 cm; PepMap, Dionex/Thermofisher). Peptides eluting from the column were ionized using an Orbitrap Elite (Thermofisher).
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bioRxiv - Cell Biology 2022Quote: ... Tissues were washed with PBS and incubated in Alexa Fluoro 488 and 647 (Invitrogen) concurrently in blocking solution for two hours at room temperature in a darkened chamber ...
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bioRxiv - Neuroscience 2021Quote: ... unilateral injections of 50-80nl of the retrograde neuronal tracer Fluoro-Gold (Molecular Probes, Eugene ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The gels were mounted on fluoro-dishes using ProLong Gold Antifade Mountant (ThermoFisher Scientific). Samples were imaged using a Nikon A1+ confocal microscope equipped with DU4 detector ...
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bioRxiv - Bioengineering 2024Quote: ... Unreacted maleimides were quenched by incubating the microgels in 100 mM N-acetyl-L-cysteine (Acros Organics) overnight at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... or rabbit anti-Tyrosine hydroxylase (1:1000, OPA1-04050, Thermo Fisher Scientific) antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ng/mL (n = 3) and 10 ng/mL (n = 3) treatment were subjected to albumin depletion according to the manufacturer’s instructions (Thermo Fisher Scientific, Loughborough, UK). Individual samples were digested with trypsin (2.5µg trypsin ...
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bioRxiv - Bioengineering 2021Quote: ... N,N,N’,N’- tetramethyl ethylenediamine and glycylglycine were purchased from Acros Organics (New Jersey, USA). Protogel 30% acrylamide ...
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bioRxiv - Microbiology 2021Quote: ... 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM, Invitrogen), calcium Ionophore A23187 (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... N,N′-Diisopropylcarbodiimide (DIC, Fisher Scientific) and 10 equivalents of DIPEA in DMF for 4 h ...
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bioRxiv - Cell Biology 2019Quote: ... with NuPAGE 3-(N-morpholino) propane sulfonic acid (MOPS) sodium dodecyl sulfate (SDS) running buffer (Invitrogen). Proteins were then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Bioengineering 2019Quote: ... n=3 pellets/condition/cell-type) and cultured in differentiation media (StemPro Chondrogenic Media; ThermoFisher Scientific) for 28 days ...
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bioRxiv - Cancer Biology 2020Quote: ... cell viability (n =3) was assayed at different times using the PrestoBlue cytotoxicity assay (Thermo Fisher), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Laser Capture Microdissection (LCM; N=3; 7 µM glass slide coated with polyethylene naphthalate – ThermoFisher #LCM0522), multiplex or regular immunohistochemistry (N≥3 4 µM glass slide ...
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bioRxiv - Neuroscience 2022Quote: Tissue cultures (n=3) were transferred as one sample into 250 μl RNAlater (ThermoFisher, Cat# AM7020) and stored at −20 °C ...
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bioRxiv - Neuroscience 2023Quote: Retinal tissues (N = 3 biological replicates) were lysed with RIPA buffer (Thermo Fisher Scientific, Cat.# 89900) containing protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl) containing N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM; Invitrogen) at a final concentration of 10 μM and incubated for 30 min in the dark on ice ...
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bioRxiv - Biochemistry 2023Quote: ... batches of 3-6 SC-islets were embedded in extracellular matrix (Geltrex, Gibco, cat. n A1569601) and cultured for 7 days under perfusion ...
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bioRxiv - Microbiology 2020Quote: ... The extraction solvent consisted of 30 g/L of tri-n-octylphosphine oxide (TOPO, Acros Organics, Geel, Belgium) in mineral oil (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... 12.5 mM Glucose and 10 μM 5’-fluoro-2’-deoxyuridine (FUDR; Thermo Fisher Scientific, 10144760), an antimitotic drug that suppresses growth of glial cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins and tyrosine-phosphorylated proteins were developed with SuperSignal Pico PLUS (Thermo Fisher) and visualized with a chemiluminometer (LuminoGraph 1 ...
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bioRxiv - Microbiology 2023Quote: ... King’s B (KB) medium was prepared by mixing 20 g/L of proteose peptone #3 (BactoTM Proteose Peptone No. 3, ThermoFisher Scientific), 1.5 g/L of dipotassium phosphate (Carl Roth GmbH + Co ...
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bioRxiv - Bioengineering 2022Quote: ... Final water-PAAm mixtures were crosslinked with 0.5% APS and 0.15% N,N,N′,N′-tetramethylethylenediamine (ThermoFisher, 17919). 50 μL of the mixtures were added to each glass and allowed to crosslink for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... on a 4-12% bis-tris gel (Genescript) in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). The gel was then fixed for 30 min in 10% acetic acid/50% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... 2′-(or-3′)-O-(N-Methylanthraniloyl) adenosine 5′-triphosphate (mant-ATP, Thermo-Fisher Scientific, Waltham, MA, USA) was serially diluted to concentrations of 5 -15 μM in assay buffer and added to the myosin at a final concentration of 1X -1.2X the final concentration of myosin ...
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bioRxiv - Microbiology 2023Quote: ... N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64) (Thermo Fisher Scientific, Waltham, MA, USA) as previously described (9) ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples (n = 3 technical replicates, each condition) were sorted with an Attune NxT flow cytometer (Thermo Fisher) and the DNA content was analyzed for the percent of cells in G1 ...
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bioRxiv - Immunology 2022Quote: ... 105 overnight cultured-T cells were incubated at 200 μL PBS solution containing 20 uM calpain substrate CMAC (t-BOC-Leu-Met, Thermo Fisher) at room temperature in dark ...
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bioRxiv - Molecular Biology 2023Quote: ... Hydroxybenzotriazole (HOBt) and O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU) were purchased from Fisher Scientific. Trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2019Quote: ... with different spectral signatures selected for the detection of PfHRP2 and PAN-pLDH were washed with distilled water and activated with Sulfo-NHS (N-hydroxysulfosuccinimide) and EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) (Pierce, Thermo Fisher Scientific Inc., Rockford, IL), both at 50 mg/mL ...
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bioRxiv - Biochemistry 2019Quote: ... The mixture was then alkylated using 3 µL of 400 mmol/L 2-chloroacetamide (CAA, Thermo Scientific; final concentration of 40 mmol/L) and incubated in the dark at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... secondary antibody was rabbit or mouse Alexa-Fluoro-488 or 568-conjugated antibody (1:1000, Invitrogen). For EdU incorporation ...
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bioRxiv - Bioengineering 2024Quote: ... consisting of 0.5 μm in diameter fluorescent polystyrene sphere latex (PSL) particles (Thermo Scientific Fluoro-Max), were aerosolized using a Collison nebulizer (CH Technologies ...
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bioRxiv - Evolutionary Biology 2024Quote: ... loaded with crystals of fluoro-ruby (dextran-tetramethylrhodamine: 10,000 MW, D1817, Thermo Fisher Scientific, MS, USA) mixed in 2% bovine serum albumin (BSA) ...
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bioRxiv - Physiology 2024Quote: ... 2 µl cDNA was amplified with TaqMan™ qPCR (38 cycles, 3 technical replicates of 3 biological replicates) using master mix (Thermo Fisher) and the following TaqMan assays ...
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bioRxiv - Biochemistry 2021Quote: ... with a 10-fold excess of N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine (IANBD-amide, Molecular Probes). After 90 min on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... to detect Phospho-Tyrosine and anti-Mouse Alexa Fluor 633 (Invitrogen, 1:500 dilution) to detect Futsch ...
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bioRxiv - Bioengineering 2023Quote: ... N,N’-Dicyclohexylcarbodiimide (DCC, 0.02 mol; Fisher Scientific) was dissolved in DCM with Norbornene-2-carboxylic acid (0.04 mol ...
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bioRxiv - Immunology 2024Quote: ... 10% heat inactivated fetal bovine serum (HIFBS) 2 × 10-3 m L-glutamine (Life Technologies), antibiotic antimycotic (1× ...
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bioRxiv - Immunology 2024Quote: ... 10% heat inactivated fetal bovine serum (HIFBS) 2 × 10−3 m L-glutamine (Life Technologies), antibiotic antimycotic (1× ...
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bioRxiv - Microbiology 2023Quote: ... the cornea containing molten scaffold medium was placed epithelial side down in a 6 well-plate containing 400 µl of Ethylene glycol-O,O’-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA) (Fisher scientific) and was kept in a CO2 (5% v/v ...