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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 200 μl of 0.25% SDS in nuclease-free water were added to each and RNA products were isolated by extraction with 250 μl of acid-phenol:chloroform (Invitrogen). After the addition of 170 mM NaCl and 15 μg glycogen (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... we used 20-30 ng of cDNA and the reaction mixture with SYBR Green nucleic acid staining (Invitrogen) on a CFX96 of CFX384 Real-Time systems (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were separated by gel electrophoresis on 4-12% Bis-Tris gradient gels using MES SDS running buffer (Invitrogen) and stained with Flamingo fluorescent protein stain (Bio-Rad) ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... The PCR product was inserted into pInducer20-CRSmut via a combined BP/LR Gateway recombination reaction utilizing pDONR221 as intermediate vector (Invitrogen, Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2021Quote: Rpp treatment (total reaction volume = 30 μl): RNA products were mixed with 20 U Rpp and 40 U RNaseOUT (Invitrogen) in 1x Rpp reaction buffer (50 mM HEPES-KOH ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were then analysed by running on a 20% non-denaturing PAGE gel in 0.5X TBE buffer (Life Technologies) for 20 min at room temperature and 200 V ...
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bioRxiv - Plant Biology 2021Quote: ... The products were cloned using the pENTR/D-TOPO Cloning Kit and transferred into pK7WG2R using Gateway LR reactions (Invitrogen). For RNAi studies ...
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bioRxiv - Genomics 2021Quote: ... 10μL of I-CeuI-digestion products was mixed in 50 μL standard Phusion polymerase reaction mix (Thermo Fisher Scientific, F530S) in GC buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and the product of the colorimetric reaction read at 750 nm in a Varioskan Flash plate reader (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were then analysed by running on a 20% non-denaturing PAGE gel in 0.5X TBE buffer (Life Technologies) for 20 min at room temperature and 200 V ...
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bioRxiv - Biochemistry 2021Quote: ... The fluorescence intensity of the FAM label was used to quantify the reaction products by peak integration with GeneMapper software (ThermoFisher) using previously reported methods (65).
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bioRxiv - Cell Biology 2022Quote: ... The purified PCR product was used as a template for the in vitro transcription reaction using the MEGAscript T7 kit (AM1334; Invitrogen). dsRNA was injected into young hermaphrodites mounted on 2 % agarose pads ...
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bioRxiv - Neuroscience 2023Quote: ... A Gateway recombination reaction was performed using BP Clonase II with the PCR product and donor vector pDONR221 (Invitrogen, #12536017) to obtain pME_hArr-TEV ...
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bioRxiv - Neuroscience 2023Quote: ... A Gateway recombination reaction was performed using BP Clonase II with this PCR product and donor vector pDONR221 (Invitrogen, #12536017) to obtain pME_hGCGR-TEVcs-QF ...
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bioRxiv - Genomics 2023Quote: ... The PCR reaction was held at 4◦C while 5µL of its product was amplified in a 15µL qPCR reaction with NEBNext High-Fidelity Master Mix and SYBR Green I (Invitrogen, S7563) at a final concentration of 0.5x ...
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bioRxiv - Molecular Biology 2023Quote: ... and the resultant hemimethylated DNA product was subsequently purified from the reaction mix by conjugation to streptavidin M280 dynabeads (Invitrogen). DNA was coupled to streptavidin beads at ∼2 μg DNA/5 μl bead slurry in bead coupling buffer (50 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0 ...
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bioRxiv - Immunology 2022Quote: ... PCR of the precipitated product (5 ng) was performed using a reaction with 5 μL SyBR green (#A25742; ThermoFisher Scientific) and 0.5 μL of 10 μM primer listed in the Supplementary table 3 ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting PCR products were purified and cloned into pDNOR201 by BP Clonase reactions (GATEWAY Cloning; Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions to generate the pDONR-cDNA vectors ...
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bioRxiv - Genetics 2022Quote: ... This PCR product was added as template to a T7 RNA polymerase transcription reaction using the Megascript T7 kit (Invitrogen). Following 3 h of RNA transcription at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... unlabelled primers were added to the reaction mixture and the purified dsDNA products were cloned into TOPO vector using the Zero Blunt TOPO Cloning Kit (Invitrogen). The construct was transformed into α-Select Gold chemically competent cells (Bioline) ...
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bioRxiv - Plant Biology 2024Quote: ... Two ul of each PCR product were used in the ligation reaction into pCR™-Blunt cloning vector (Invitrogen™) and Sanger sequenced for polymorphisms analysis.
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bioRxiv - Cancer Biology 2024Quote: ... Polymerase chain reaction (PCR) products were obtained and quantified using Maxima SYBR Green qPCR Master Mix (K0223, Thermo Fisher Scientific) in a final reaction containing 20 ng of cDNA and 0.5 μM of each of the following primer pairs for murine genes ...
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bioRxiv - Biochemistry 2024Quote: ... Transcription and poly(A) tailing reactions were performed with these PCR products using mMESSAGE mMACHINE™ T7 Transcription (ThermoFisher #M0531S) and Poly(A ...
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bioRxiv - Genetics 2021Quote: ... PCR product was separated on a 2.5% agarose gel and stained with SYBR Green I nucleic acid gel stain (ThermoFisher, S7563) for band visualization using a BioRad Gel Doc EQ system ...
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bioRxiv - Synthetic Biology 2024Quote: ... The final products of the splint ligations were visualised on 8% UREA-PAGE using SYBR Gold nucleic acid gel stain (Invitrogen). For prolonged storage ...
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bioRxiv - Genetics 2021Quote: ... The microsatellite marker Xpsp3000 was run using polymerase chain reaction (PCR) products and were separated on an ABI3730XL DNA fragment analyzer (Applied Biosystems), and alleles were scored with GeneMarkerv4.0 software (SoftGenetics) ...
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bioRxiv - Microbiology 2022Quote: ... approximately 800 ng of DNA products were subjected to 20 µL in vitro transcription reactions according to the manufacturer’s instructions (MEGAscript T7 kit, Ambion/Thermo Scientific). After incubating the samples at 37 °C for 3 h and 45 min ...
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bioRxiv - Genomics 2020Quote: ... were amplified via the polymerase chain reaction (PCR) using primers to facilitate subsequent ligation of the PCR products into the glutathione-S-transferase encoding vectors (Life Technologies). The M.MspI PCR product was initially inserted into the BamHI and EcoRI sites in pGEX 2T ...
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bioRxiv - Molecular Biology 2022Quote: ... Substrates and reaction products were separated by agarose gel electrophoresis and the gel was stained with SYBR-Gold (S11494, Thermo Fisher).
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bioRxiv - Microbiology 2024Quote: ... A volume of 3.5 µL of the cDNA product was used in a 50 µL PCR reaction with Phusion™ High-Fidelity DNA Polymerase (2 U/µL) (ThermoFisher). Three universal Influenza A primers at a 0.20 μM concentration were used in the PCR reaction ...
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bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
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bioRxiv - Plant Biology 2024Quote: ... These PCR products were introduced into pDONR221 by BP reactions to produce four sGFP-tubulin entry vectors (Thermo Fisher, MA, USA). We performed the LR reaction of the pGWB501 destination vector with sGFP-tubulin entry vectors carrying pTUA1:sGFP-TUA1 ...
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bioRxiv - Immunology 2023Quote: ... DNA products were used for real time qRT-PCR reaction using the TaqMan 2X Universal PCR Master Mix (Thermo Fisher Scientific) on an iQ5 RT-PCR detection system (BioRad ...
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bioRxiv - Genetics 2024Quote: ... PCR products were cleaned via ethanol precipitation and 2.5 µL clean product used in a cycle sequencing reaction (BigDye© Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific)) containing 1X Ready Reaction Premix ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR reactions were carried out using Maxima SYBR Green/ROX qPCR MM (Thermo Scientific, product no. K0221, Rockford, IL, USA) in an Applied Biosystems StepOne Plus™ Real-Time PCR System (Foster City ...
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bioRxiv - Physiology 2024Quote: Absorbance increase caused by enzymatic reaction products was measured at 340 nm using the Multiskan FC microplate reader (Thermo Scientific, USA). Trehalose and ethanol levels were calculated based on respective calibration curves and recalculated to original concentrations ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2 kb) was visually checked by running 1:10 of the PCR reaction product on a 1% (w/v) agarose (Invitrogen 1015) gel ...
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bioRxiv - Biochemistry 2024Quote: Reaction substrates and products were quantified using HPLC coupled to a UV-vis detection unit (Dionex Ultimate 3000 Systems, Thermo Scientific). Twenty microliters sample were injected from an autosampler cooled to 10°C into a Nucleoshell RP 18plus column (particle size 5 μm ...
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bioRxiv - Biochemistry 2024Quote: 6xHis-Nβ products produced from enzymatic reactions with Notch variants were isolated by immunoprecipitation with Dynabeads His-tag Isolation & Pulldown (Invitrogen #10103) in 10 mM MES (2-(4-morpholino ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PCR products were visualized via agarose gel electrophoresis and nucleic acid staining with SYBR safe DNA stain (S33102, Thermo Fisher Scientific), and imaged using an EZ Gel Documentation System (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... MF002 - plus-10) and the PCR products were fractionated on 8% TBE gel stained with SYBR Gold nucleic acid stain (Invitrogen, S11494). Quantitative PCR (qPCR ...
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bioRxiv - Physiology 2024Quote: The PCR products were separated by agarose gel electrophoresis and visualized using SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher Scientific). Images were captured with a WUV-M20 Printgraph Classic UV transilluminator (ATTO CORPORATION ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were verified by 1.5% TAE-agarose gel electrophoresis with SYBR Green I nucleic acid stain (Invitrogen, Thermo Fisher Scientific). The PCR products were purified using a GenepHlow Gel/PCR Kit (Geneaid).
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bioRxiv - Microbiology 2024Quote: ... PCR products were verified by 1.5% TAE-agarose gel electrophoresis with SYBR Green I nucleic acid stain (Invitrogen, Thermo Fisher Scientific). The PCR products were purified using a GenepHlow Gel/PCR Kit (Geneaid).
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bioRxiv - Microbiology 2021Quote: ... lysates were spun at 10,000 x g for 10 minutes at 4 °C prior to reaction with 4- acetamido-4’-maleimidyl-stilbene-2,2’-disulfonic acid (AMS) (ThermoFisher Scientific). AMS alkylation was performed by vortexing the lysates in 15 mM AMS ...
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bioRxiv - Molecular Biology 2022Quote: ... The C-TPF1 fragment (TPF1 amino acids 423-697) was amplified from pENTR-TPF1 and cloned into a pDONR207 plasmid by PB reaction (Invitrogen). Plasmids were transferred to pGADT7 and pGBKT7 vectors (Clontech ...
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bioRxiv - Immunology 2020Quote: ... was added for 15 min at room temperature and the reaction was quenched with 50 μL stop solution containing 0.16N sulfuric acid (ThermoFisher, #N600). The plates were read in a spectrophotometer (BioTek Instruments Inc. ...
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bioRxiv - Immunology 2024Quote: ... permeabilised with 0.5% Triton x-100 and OPP incorporation into newly synthesised peptides was measured by conjugating with Alexa647-azide via a Click-IT chemistry reaction (2 µM Alexa-647-azide, 2 mM CuSO4, 5 mM ascorbic acid in PBS) (Invitrogen) and assessing fluorescence by flow cytometry.
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bioRxiv - Microbiology 2024Quote: ... Extracted nucleic acids were first subjected to a reverse transcription (RT) reaction using the High-Capacity cDNA Reverse Transcription kit (ThermoFisher Scientific ...