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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and heart were homogenized in 600 μL 2% FBS (Gibco, MA)/MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Bacteroides were cultured in Brain heart infusion (BHI) medium (Fisher Scientific) supplemented with 5 µg/ml Hemin and 1 µg/ml vitamin K3 ...
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bioRxiv - Microbiology 2023Quote: ... Strains were cultured in brain heart infusion (BHI, Fisher Scientific, NH) at 37 °C under different oxygen conditions as specified in the main text ...
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bioRxiv - Immunology 2022Quote: ... fetal heart and fetal liver samples were stored in RNAlater (Invitrogen) at −80°C until processing ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from the bottom halves of infarcted or sham adult hearts and primary cardiomyocytes using TRIzol® reagent (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... total protein extracts were prepared from hearts solubilized in radioimmunoprecipitation assay (RIPA) buffer supplemented with a combined protease and phosphatase inhibitor cocktail (Thermo Scientific). Mitochondrial protein extracts were prepared by solubilizing isolated cardiac mitochondria in the same RIPA buffer ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA from the heart tissues was extracted using the TRIzol reagent (RNA STAT 60 Tel-Test Ambion, Austin, TX, USA). Complementary DNA (cDNA ...
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bioRxiv - Immunology 2024Quote: ... For tissue parasite quantification, genomic DNA was purified from 50 μg of tissue (spleen, liver, SM and heart) using TRIzol Reagent (Life Technologies) following manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from rats’ hearts with use of the Gene Jet PCR Purification Kit (Thermo Fisher Scientific, Waltham, MA) and quantified with a Nano-Drop One spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... porcine brain capillaries were seeded in T75 flasks (Thermo Fisher Scientific, Roskilde, Denmark) coated with collagen IV (500 μg/ml ...
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bioRxiv - Evolutionary Biology 2022Quote: ... rehydrated with BICAM containing 0.5 μg MS-grade porcine trypsin (Pierce Thermo Scientific), and incubated overnight at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Blood collected for flow cytometric analysis was isolated from the heart prior to transcardial perfusion and transferred into 1x PBS + EDTA (Thermo Fisher Scientific). Blood was then processed with RBC lysis and resuspended in cold cRPMI prior to staining and fixation for flow cytometry ...
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bioRxiv - Cell Biology 2023Quote: ... NRVMs were isolated and cultured as previously described.23 The hearts from D0-3 rat pups were isolated and rinsed in HBSS (ThermoFisher Scientific, 14025092) before placed in trypsin-EDTA 0.05% (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The border zone was roughly dissected and a pool of border zone cells from 8 hearts was dissociated using the Pierce Primary Cardiomyocyte Isolation kit (Thermo Fisher 88281) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... total protein extracts were prepared from embryonic hearts solubilized in radioimmunoprecipitation assay buffer supplemented with combined protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific). Proteins were resolved on 10% SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were extracted from pulverized hearts using 1x RIPA Buffer with addition of protease and phosphatase inhibitor cocktail (HALT, Thermo Fisher Scientific) and deacetylase inhibitors (10 μM Trichostatin A (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Hearts were digested with a collagenase solution (Collagenase Type I, Life Technologies) followed by differential plating ...
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bioRxiv - Pathology 2022Quote: Heart and lung tissue was fixed in SafeFix solution (Thermo Fisher Scientific), embedded ...
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bioRxiv - Microbiology 2022Quote: ... which consisted of base Brain Heart Infusion medium (BHI; Gibco, Gaithersburg, MD) containing 0.5% wt/vol yeast extract (Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... Heart tissues were harvested and embedded with OCT (Thermo Scientific™, USA). The 8μm thick tissues were subjected to TUNEL staining according to the manufacturer’s instructions for an In-Situ Cell Death Detection Kit (Cat ...
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DROSOPHILA MTOR COMPLEX 2 PRESERVES MITOCHONDRIAL AND CARDIAC FUNCTION UNDER HIGH FAT DIET TREATMENTbioRxiv - Genetics 2021Quote: ... Hearts were mounted in ProLong Diamond antifade reagent (Thermo Fisher Scientific, P36361) before being imaged using a FV3000 Confocal Laser Scanning Microscope (Olympus).
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bioRxiv - Genomics 2021Quote: ... “Heart media” is composed of RPMI (15-040-CM, Thermo Fisher Scientific) with B27 Supplement minus insulin (A1895601 ...
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bioRxiv - Molecular Biology 2022Quote: ... Anti-HA magnetic beads (88836; Thermo Fisher Scientific; 100 μl per heart) were washed with 1,000 μl polysome lysis buffer three times ...
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bioRxiv - Cancer Biology 2023Quote: Ground heart tissue powders were collected and soaked in Trizol (Invitrogen, USA) and RNeasy Fibrous tissue mini kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos or hearts were dissected in cold PBS (Life Technologies, CAT# 14190250), de-yolked and placed in PBS on ice until dissociation ...
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bioRxiv - Physiology 2023Quote: Murine hearts were perfused and fixed with 4% PFA (28908, Thermo Fisher) for 3 hours and subsequently washed with PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: Amoebidium isolates were grown on Brain Heart Infusion (10% BHI, ThermoFisher CM1135) liquid media at 25°C in 25 mL culture flasks ...
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bioRxiv - Cell Biology 2024Quote: ... Hearts were washed with ice-cold HBSS containing 1% penicillin-streptomycin (Gibco) for 5 min to remove blood ...
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bioRxiv - Microbiology 2024Quote: ... frozen in brain heart infusion + 0.5% yeast and 30% glycerol (Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... the cultures were grown in Brain Heart Infusion (BHI) broth (Fisher Scientific). When necessary ...
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bioRxiv - Pathology 2024Quote: ... hearts were transversely sectioned using a cryotome (Thermo NX50; Thermo Fisher Scientific) rinsed quickly in 1 change of acetic acid solution prior to mounting in 95% alcohol with 2 changes in Histoclear solution (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... Heart nuclei were stained with Sytox red (S34859, Thermo Fisher, Waltham, MA) and sorted and purified through fluorescence-activated cell sorting (FACS) ...
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bioRxiv - Biochemistry 2020Quote: ... Porcine mucosal heparin was biotinylated at the reducing end using hydroxylamine biotin (ThermoFisher, UK) as described by Thakar et al ...
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bioRxiv - Cell Biology 2021Quote: Acrylamide gels (10%, v/v) containing 0.2% (w/v) porcine gelatin (Thermo Fisher Scientific) were resolved with a Triton X-100 buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Porcine mucosal heparin was biotinylated at the reducing end using hydroxylamine biotin (ThermoFisher, UK) as described by Thakar et al ...
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bioRxiv - Biochemistry 2020Quote: ... Porcine mucosal heparin was biotinylated at the reducing end using hydroxylamine biotin (ThermoFisher, UK) as described by Thakar et al ...
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bioRxiv - Genetics 2023Quote: ... and heart from 6 wild-type crucian carp at 4-month-age were dissected for total RNA extraction using Trizol (Thermo Fisher, CA, USA). RNA quality was measured using Nanodrop 8000 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized from 150 ng of total RNA extracted from heart tissues with use of a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Framingham, MA). RT-PCR was performed with QuantStudio (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: Heart were dissociated using 2 mg/mL type IV collagenase (Gibco™, ThermoFisher) for 1 hour at 37°C and the resulting dissociated cells were filtrated on a 30 µm strainer ...
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bioRxiv - Physiology 2020Quote: Heart were dissociated using 2 mg/mL type IV collagenase (Gibco™, ThermoFisher) for 1 hour at 37°C and the resulting dissociated cells were filtrated on a 30 µm strainer ...
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bioRxiv - Cell Biology 2021Quote: ... or goat anti-rabbit Alexa Fluor 488 (hearts) (Cat. No. A-32731; Invitrogen) diluted 1:100 in 1% BSA/PBSTX ...
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bioRxiv - Biochemistry 2021Quote: Human heart samples were homogenised in RIPA buffer (Thermo Scientific, Cat. No. 89901) supplemented with Complete Mini ...
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bioRxiv - Biochemistry 2020Quote: ... Other media used for bacterial growth were brain-heart infusion broth (BHI) (Gibco) and Double Yeast Tryptone medium (DYT ...
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bioRxiv - Microbiology 2021Quote: ... A sweep of colonies was inoculated into brain heart infusion (BHI) broth (Thermofisher) and grown statically overnight at 37°C ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ... hearts were dissociated using 2 mg/mL type IV collagenase (Gibco™, ThermoFisher) for 1 hour at 37°C and the resulting dissociated cells were filtrated on a 30 µm strainer ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ... hearts were dissociated using 2 mg/mL type IV collagenase (Gibco™, ThermoFisher) for 1 hour at 37°C and the resulting dissociated cells were filtrated on a 30 µm strainer ...
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bioRxiv - Physiology 2024Quote: ... coverslips were mounted on heart sections using ProLongTM Gold antifade reagent (#P36930, Invitrogen). Images were taken on a Zeiss LSM880 Airyscan confocal microscope and analyzed using the software ImageJ.
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bioRxiv - Developmental Biology 2024Quote: ... hearts were incubated with secondary antibodies diluted to 1/1000 (ThermoFisher Scientific, Invitrogen) in PBSGT during 2 days at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... hearts were incubated with secondary antibodies diluted to 1/1000 (ThermoFisher Scientific, Invitrogen) in PBSGT during 2 days at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Fluorescent microtubules were polymerized from 4 mg/ml porcine tubulin (80% unlabeled and 20% Alexa Fluor 647 NHS ester-labeled; Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA) for 2 h at 37 °C in BRB80 (80 mM PIPES ...