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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for BD 3 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... primary mouse cortical glial isolated from postnatal day 3 ICR pups were added to the transduced cultures in MEM (Life Technologies) and 10% donor equine serum (HyClone) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and AlexaFluor (AF488, AF568) conjugated anti-rabbit or anti-mouse antibodies (#A-11008, A-11004 Fisher Scientific) were added and incubated for 1h at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... The coverslips were washed 3 x in PBS and labeled with donkey anti-mouse AlexaFluor 647 (1:1000, A31571, Thermo Fisher), for 1 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked again and incubated with 2-3 drops of an anti-mouse poly-HRP-conjugated secondary antibody from the Invitrogen Tyramide SuperBoost secondary staining kit (ThermoFisher, B40912). Samples were rinsed three times with 1X PBS and the tyramide working solution conjugated to AlexaFluor 488 was added and allowed to react for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was for 3 h with Alexa Fluor 647 goat anti-mouse IgG (1:1000, Thermo Fisher Scientific, A21236) and Alexa Fluor 488 goat anti-rabbit IgG (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Slides were again washed 3 times with 50 mL of PBS and incubated with anti-mouse IgG-Alexa 488 secondary antibody (1:1000; ThermoFisher A28175) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then washed with cold 1X PBS three times for 3 min each and incubated with Alexa 555-labeled anti-rabbit and Alexa 488-labeled anti-mouse secondary antibody (Thermo Fisher) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 3 times with PBS-T and incubated with 1.6 μg/ml biotin-conjugated goat anti-mouse IgM (Thermo Fisher Scientific) or 4 μg/ml biotin-conjugated goat anti-mouse IgG1 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (AlexaFluor 546 donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher). Secondary antibody was dissolved in 1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...
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bioRxiv - Bioengineering 2023Quote: ... and detection antibodies goat anti-mouse-Alexa Fluor 647 (Cat. #A21240) and goat anti-rabbit-Cy3 (Cyanine 3, Cat. #A10520) were obtained from Fisher Scientific.
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSCs were seeded on CF1 mouse embryonic feeders and maintained for 3 days in ESC medium (KO-DMEM (ThermoFisher, 10829-018), 20 % KO-Serum (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... 10 mins) and resuspended in 3 mL MAGIC buffer supplemented with 60 µL Rabbit anti-Mouse IgM FITC Secondary Antibody (Invitrogen, 31557). The sample was gently rocked at 4 °C for 20 hrs ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were successively incubated with primary antibodies diluted in blocking buffer (mouse anti-G3BP1, 1:4000, BD Biosciences, rabbit anti-GFP, 1:1000, ThermoFisher, mouse anti-PKR ...
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bioRxiv - Cell Biology 2020Quote: ... EndoB1 siRNA 5’-UGUUUAUACGACUUGGAGCUU-3’ and 3’-AAGCUCCAAGUCGUAUAAACA-5’ (Invitrogen), control siRNA (Ambion) ...
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bioRxiv - Microbiology 2021Quote: ... FluoZin-3 (FluoZin™-3, AM, cell permeant, Thermo Fisher) was added at 1 mM ...
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bioRxiv - Immunology 2020Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific). The activated beads were washed three times with 50 mM MES pH 5.0 and added to SARS-CoV-2 S protein which was diluted in 50 mM MES pH 5.0 ...
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bioRxiv - Immunology 2022Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific) and incubated for 30 min on a rotator at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... siPALS1 (5’-UUCCUUAUGAUGAACUGGCtt-3’) and siPATJ (5’-CCAGAUACUCACACUUCAGtt-3’, Ambion), siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (Invitrogen, D383) was used ...
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bioRxiv - Developmental Biology 2023Quote: ... mounting 3 dpf embryos in 3% methylcellulose (Thermo Scientific, 258111000). After imaging ...
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... 3 (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were then washed 3 times with PBS + TritonX and a secondary anti-mouse antibody Alexa Flour 488 (Life Technologies: 1:1000) was added for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Fifteen μl of a solution at 3 µg/mL of mouse anti-AP (IgGAb-1 clone 8B6.18 Thermo Fisher Scientific; Waltham, MA) in 1X PBS was added to each well of 384-well plates using an automated multichannel pipette (Viaflo Assist ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of RNAPII was done using 3 μL of Rpb3 antibody (W0012 from Neoclone) coupled to magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific). ChIP and Input samples were labeled with mono-reactive NHS ester fluorescent Cy5 and Cy3 dyes (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of RNAPII was done using 3 μL of Rpb3 antibody (W0012 from Neoclone) coupled to magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific). ChIP DNA was analyzed by qPCR using primers targeting the 5’ (forward ...
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bioRxiv - Microbiology 2019Quote: ... The slides were then washed with TBST for 3 minutes thrice before adding 1:100 goat anti-mouse IgG-AlexaFluor488 (ThermoFisher, A-11029) or 1:250 goat anti-rabbit IgG-AlexaFluor647 (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... The next day samples were washed 3 times with 1X PBS and incubated with secondary antibody (goat anti- mouse IgG H+L Alexa Fluor 594 (Invitrogen, A-11032)) diluted in blocking buffer for 1 hr at room temperature in the dark ...
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bioRxiv - Microbiology 2019Quote: ... JE-CVax hyper-immune and non-immune heat inactivated mouse serum samples (starting dilution of 1/9) were serially diluted 3-folds in RPMI 1640 medium (Gibco, ThermoFisher Scientific) supplemented with 4% low IgG serum ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...
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bioRxiv - Physiology 2020Quote: ... and PCR was performed on the QuantStudio 6 Flex system (3 technical replicates per mouse, Applied Biosystems, Iowa Institute of Human Genetics). Gene expression was calculated using the ΔΔct method (39 ...
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bioRxiv - Genetics 2022Quote: ... Total protein was harvested after 3 days of culture and analyzed by Western blot (mouse anti-DsRed, Clontech; rabbit anti-POU6F2, Invitrogen, PA5-35115). Band intensities were measured using ImageJ software to quantify expression.
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bioRxiv - Neuroscience 2022Quote: ... For KV2.2 channels, NDS was substituted for normal goat serum (NGS, 3%) and Alexa Fluor® 555 goat anti-mouse IgG1 (Thermo Fisher Scientific Cat# A-21127 ...
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bioRxiv - Neuroscience 2023Quote: ... The next day RGCs were washed 3 × 5 minutes with PBS and incubated with secondary antibodies: goat anti-mouse Alexa 488 (Thermo Fisher A11029) 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and blocked in 3% milk– PBS-T prior to incubation with serial dilutions of serum or the mouse IgG standard (Invitrogen, Jackson ImmunoResearch) in 1% milk-PBS-T for 2 hrs at RT ...
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bioRxiv - Physiology 2024Quote: ... R-5’-3’ UGGUUCAUCAGCCUUUAACTT) and mock siRNA (General Biosystems, China) were transfected into mouse DCT cells by Lipofectamine™ 3000 (Invitrogen, L3000015) (20 nM ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were rinsed three times with TBS prior to incubation with donkey-anti-mouse-cyanine (Cy)3 secondary antibody (Invitrogen, Carlsbad, USA) combined with phalloidin-Alexa Fluor 488 (Santa Cruz Biotechnologies ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... and 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) (Invitrogen) in water for 1 hour at 60°C ...
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bioRxiv - Neuroscience 2019Quote: ... Fluo-3-acetoxymethylester (Fluo-3-AM) (Molecular Probes-Thermo Fisher Scientific) was used (Beauvais et al. ...
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bioRxiv - Neuroscience 2019Quote: ... Fluo-3-acetoxymethylester (Fluo-3-AM) (Molecular Probes-Thermo Fisher Scientific) was used (Beauvais et al. ...
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bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Immunology 2022Quote: ... 1.9g of ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (ThermoFisher #22980) and 1.2g of N-hydroxysuccinimide (NHS ...