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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for 2 Pyridinesulfonic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Monomeric (G)-actin was fluorescently labeled with AlexaFluor 488 carboxylic acid succimidyl ester (Invitrogen) (62) ...
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bioRxiv - Developmental Biology 2022Quote: Osteoblasts on glass coverslips were loaded with 2 µM Fura-2 AM-ester (Invitrogen) for 30 minutes at 25 °C in 107 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: Fura-2 acetoxymethyl ester (fura-2/AM) was from Molecular Probes (Leiden, The Netherlands). High-glucose Dulbecco’s modified Eagle’s medium (DMEM) ...
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bioRxiv - Biophysics 2020Quote: ... This initial PEG treatment was followed by a secondary PEG treatment with 25mM short-chain 333 Da MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific). These treatments resulted in a biotin-coated surface on the slide ...
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bioRxiv - Biophysics 2023Quote: ... then followed by a secondary PEG treatment with 25 mM short-chain 333 Da MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific). A microfluidics chamber was constructed on the slide ...
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bioRxiv - Biophysics 2024Quote: ... the slides were treated with a short-chain PEG solution (25 mM short-chain 333 Da MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific) in 0.1 M sodium bicarbonate ...
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bioRxiv - Cell Biology 2024Quote: The mitochondrial membrane potential was measured using tetramethylrhodamine methyl ester (TMRM) from the MitoProbe™ TMRM Assay Kit (Thermo Scientific™). Cells were harvested and incubated with TMRM at 37°C for 20 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... methyl tert-butyl ether and 2-propanol were from Thermo Fisher Scientific (Pittsburg ...
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bioRxiv - Microbiology 2024Quote: ... Methyl-esterified free fatty acids were analyzed using a ThermoFisher Exactive GC mass spectrometer (ThermoFisher), and ions were introduced by electron impact ionization (70 eV) ...
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bioRxiv - Immunology 2021Quote: ... human cGAS was labelled with AlexaFluor-488 (AF488) carboxylic acid (succinimidyl ester) (Thermo Fisher Scientific) according to manufacturer’s manuals using a molar ratio of 1:10 at 4°C for 4 h ...
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bioRxiv - Bioengineering 2020Quote: ... Alexa Fluor 555 carboxylic acid succinimidyl ester was obtained from Life Technologies (Carlsbad, CA, USA). Protein samples were labeled with Alexa Fluor 555 carboxylic acid succinimidyl ester according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... live staining of retinal landmarks was performed by incubating live zebrafish embryos in 100 nM solution of Bodipy TR methyl ester (Thermo Fisher Scientific) in E3 embryo rearing media for 1 h at room temperature following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen) at 2 mg/ml in PBS for 10 min or with Laurdan (see above ...
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bioRxiv - Cell Biology 2023Quote: ... 2- & 3-methyl pentane and n-hexane (Thermo Scientific, Waltham, MA, USA). Reported compounds detected by the GC-MS were confirmed by matching retention times and mass–charge (m/z ...
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bioRxiv - Molecular Biology 2021Quote: ... cryosectioned zebrafish larvae were washed 3x in wash buffer for 5 min and subsequently stained with Filipin solution for 60 min in a humidified dark chamber and co-stained with BODIPY TR methyl ester (1:300, Life Technologies, Darmstadt, Germany). Staining solution was removed and slides were washed twice in wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... samples were stained with CellTrace BODIPY TR methyl ester (1:200 in PDT [1 % DMSO, 0.1 % Triton X-100 in PBS], cat# C34556, Thermo Fisher Scientific, Waltham, MA) for 20 min at RT and/or DAPI (5 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: The mitochondrial membrane potential was measured using the tetramethylrhodamine methyl ester (TMRM, 50 nM, Life technology, T668) and Mitotracker Green (100nM, Thermo Fisher Scientific, M7514) fluorescent probes according to the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was aspirated and the cell pellet was resuspended in 150 μL KCl buffer (for composition, see Ca2+ uptake protocol above) supplemented with 500 nM tetramethylrhodamine methyl ester (TMRM, Thermo Fisher Scientific, I34361) and 0.005% digitonin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... then labelled with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluoresceinacetoxymethyl ester (Life Technologies, UK). Neutrophils were then added to wells under normoxia or hypoxia ...
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bioRxiv - Physiology 2023Quote: ... cardiomyocytes were incubated with 1 µM Fura-2- acetoxymethyl ester (Fura-2 AM, cell permeant, ThermoFisher) and 0.075% Pluronic F-127 (20% in DMSO ...
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bioRxiv - Neuroscience 2024Quote: iPSC-derived neurons were incubated in 2.5 μM Fura-2-acetoxymethyl ester (Fura-2-AM, Invitrogen) for 50 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with 4 μM fura-2 acetoxymethyl ester (fura-2/AM, Invitrogen, Cat# M1291) in HEPES-buffered solution (137 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with 1 μM Fura-2 acetoxymethyl ester (Invitrogen, F1221) for 30 minutes at 37°C in NM medium ...
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bioRxiv - Developmental Biology 2024Quote: ... NHS-ester(Succinimidyl Ester) with Alexa 555 (A20009, Invitrogen) was added to the collagen stock at 1:1000 the day before ...
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bioRxiv - Neuroscience 2023Quote: All fly lines were maintained in mixed sex groups in bottles on JazzMix media or our own food made following the same recipe (brown sugar, corn meal, yeast, agar, benzoic acid, methyl paraben and propionic acid; Fisher Scientific, Whitby, ON, Canada) at 25°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Mature sensory neurons in 8-well μ-slide chambers were co-stained with 10 nM tetramethylrhodamine methyl ester (TMRM; Life Technologies, Grand Island, NY) and 100 nM MitoTracker Green FM (Life Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Each well was then further passivated by adding 9 μL of a freshly prepared aqueous solution of methyl-PEG4-NHS-Ester (10 mg/mL, Thermo Fisher, Cat. No. 22341), followed by adding 1 μL of 1 M NaHCO3 (pH 8.5) ...
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bioRxiv - Physiology 2024Quote: ... Upon full differentiation myotubes were incubated for 30 minutes with 250 nM tetramethyorhadamine methyl ester perchlorate (TMRM) (T668, Thermo Fisher Scientific, Waltham, MA, USA), Hoechst 34580 (1 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: The cultured cells were incubated with Fura-2-acetoxymethyl ester (Fura-2-AM; Molecular Probes; 2.5 µM) in MEM (0.7 mL ...
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bioRxiv - Neuroscience 2024Quote: ... 2’,7’- Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetomxymethyl Ester (BCECF-AM, 5 µM) from Invitrogen. All drugs were dissolved in water and added to the standard aCSF except BCECF-AM incubated in Pluronic® F-127 (0.02% ...
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bioRxiv - Cell Biology 2024Quote: ... N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (Gibco), sodium pyruvate (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (Gibco), sodium pyruvate (Gibco) ...
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bioRxiv - Bioengineering 2021Quote: Protein supernatants were then labelled with Alexa Fluor® 555 carboxylic acid succinimidyl ester (AF555) (ThermoFisher Scientific) essentially as previously described [76] ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescent G-actin was prepared by labelling G-actin with AlexaFluor 488 carboxylic acid succimidyl ester (Invitrogen, through Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: hSSB1 and INTS3 proteins were labeled with AF647 (Alexa Fluor 647 carboxylic acid succinimidyl ester, Thermo Fisher). Proteins were dialyzed against Labeling buffer (50 mM MES pH 6.5 ...
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bioRxiv - Biophysics 2022Quote: ... NPE-caged ATP (adenosine 5’-triphosphate, P3- (1-(2-nitrophenyl ethyl) ester) (Invitrogen) dissolved in attachment buffer was photolyzed in the AFM chamber using an UV light source at 340 nm ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were loaded with Fura-2 acetoxymethyl ester (Molecular Probes, Eugene OR). (2 μM ...
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bioRxiv - Neuroscience 2023Quote: ... we used Fluo-2 acetoxymethyl (AM) ester (Thermo Fisher Scientific, Waltham, MA, USA) as the fluorescent calcium indicator to monitor the intracellular calcium levels in primary cultured cortical neurons ...
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bioRxiv - Genetics 2023Quote: ... each coverslip was incubated in 3 μl fura-2 acetoxymethyl (AM) ester (Invitrogen) in 1X HBSS containing 5 mg/ml BSA (Sigma- Aldrich ...
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... The NHS-Ester staining (Thermofisher, DyLight™ 488 NHS Ester) was used at 5μg/mL and incubated 1 hour in PBS ...
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bioRxiv - Microbiology 2021Quote: ... 2% Non-essential amino acids (Gibco), 6% Sodium bicarbonate solution (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... 2% Non-essential Amino Acids (Gibco), 2.5% 1M HEPES (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2 mM glutamic acid (Gibco) for 45 min at 37°C without CO2 ...
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bioRxiv - Microbiology 2023Quote: ... 2% non-essential amino acids (Gibco), 1% glutamine (Gibco) ...
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bioRxiv - Genetics 2023Quote: ... 2% B27 without retinoic acid (GIBCO), 1% N2 supplement (GIBCO) ...
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bioRxiv - Biophysics 2022Quote: ... succinimidyl ester (Invitrogen), as previously described [59] ...
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bioRxiv - Cell Biology 2023Quote: ... succinimidyl ester (Invitrogen) was mixed with fibrinogen solution in a 7.5:1 molar ratio for 1 hour at room temperature and then filtered through a HiTrap desalting column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial membrane potential of primary neurons was measured by fluorescent staining of Tetramethylrhodamine methyl ester (Image-iT™ TMRM Reagent, ThermoFisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The mitochondrial membrane potential and dysfunctional mitochondria were measured by staining BM cells with Tetramethylrhodamine Methyl Ester (TMRM 500 nM, Thermo Fisher Scientific, Cat. No. T668), MitoTracker Green (100 nM ...
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bioRxiv - Immunology 2023Quote: ... cells treated with LP were loaded with 2 μM of the acetoxymethyl ester form of Fura-2 (Invitrogen) in RPMI-1640 at 37°C for 30 min and fluorescence data were analyzed using MetaFluor (Molecular Devices ...