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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Recombinant Rabbit FGF2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... cells were serum-starved (with 0.3% SCS) for 72 hours prior to induction of gene expression with 0.1µg/mL recombinant EGF (Gibco) or PBS for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: Recombinant PfbA (rPfbA) or BSA was coated onto 0.5-µm-diameter fluorescent beads (FluoroSphere, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 ng of poly(dI-dC) were used for 100 ng of recombinant ERα (ThermoFisher, RP-310) and 1 µg for 10 µg of nuclear extracts ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... and 10 μg/mL Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 555 Conjugate (CTSB, Fisher Scientific) to label GM1 as the acceptor ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated with 100 U/mL mouse recombinant IFN-γ (Invitrogen, Carlsbad, CA, USA) for 24 hours and subjected to flow cytometry analysis of PD-L1 expression as described above.
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel droplets were overlaid with recombinant organoid medium: Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were prepared in Spodoptera frugiperda (Sf9) cells using the Bac-to-Bac system (Life Technologies). Recombinant protein complexes were expressed in Spodoptera frugiperda by co-infection of single baculoviruses ...
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bioRxiv - Neuroscience 2021Quote: ... was added to each sample (4μl 5X First-Strand Buffer, 1μl 0.1 M DTT, 1μl RNaseOUT Recombinant RNase Inhibitor (Invitrogen; 10777019), 1μl of SuperScript III RT (200 units/μl) ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... fibroblast outgrowths were dissociated and split 1:4 using a recombinant trypsin-like enzyme (TrypLE Select, Invitrogen). Additional fibroblast preparations were commercially available ...
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bioRxiv - Immunology 2021Quote: ... The recombinant Fab was purified from culture supernatant using an anti-CH1 CaptureSelect column (Thermo Fisher Scientific). For the RBD/COV2-2196 complex ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant Rep1ΔAD-His and its variants were incubated with 50 μl D–Galactose agarose beads (Thermo Scientific) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
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bioRxiv - Molecular Biology 2022Quote: ... coli expression plasmids for purification of recombinant Cdc14 enzymes were generated using the Gateway cloning system (Invitrogen). S ...
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bioRxiv - Microbiology 2022Quote: ... and 50 seconds elongation at 72°C with 1U of Pfu recombinant DNA polymerase (Thermo Fisher Scientific). The amplicon ORF45HA was cut with NheI/SmaI and inserted in pEGFP-C1 (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... recombinant baculoviruses were generated in Sf9 cells using the Bac-to-Bac baculovirus expression system (Invitrogen, USA). HEK293F cells were cultured under 5% CO2 at 37 °C and were used for transfection at a density of 2.5 × 106 cells/ml ...
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bioRxiv - Plant Biology 2023Quote: ... All PCRs were performed by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) and carried out in a total volume of 25 µL containing the following ...
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bioRxiv - Microbiology 2023Quote: ... 15μL of annealed guide RNA oligos were mixed with 15μL of 1μM recombinant Cas9 (IDT) in 220μL of Opti-MEM media (Gibco) and incubated for 5min at room temperature ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant adenoviruses expressing the GRK2 shRNA were produced using BLOCK-iT™ Adenoviral RNAi Expression System (Invitrogen) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... The Dicer/TRBP enzyme was the mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... Protein concentrations were determined using Pierce 660 nm protein assay (Thermo Scientific). Extracts with 500 μg of protein were alkylated with 14 mM chloroacetamide (CAA ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentration was measured by EZQ protein quantification kit (Thermo Fisher Scientific), and 30 µg of protein from each sample was reduced with 10 mM dithiothreitol overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured by BCA Protein assay kit (Thermo Fisher Scientific) at 562 nm using BSA to produce a standard curve ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) and purity was checked by sodium-dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined by a BCA Protein Assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: Protein concentration was determined using a BCA protein assay kit (ThermoFisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The amount of protein was determined using QuBit Protein Assay Kit (ThermoFisher) following the manufacturer’s instruction and 80 ug of protein was loaded per well ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A beads (Dynabeads Protein A, ThermoFisher Scientific, Waltham, MA, Cat #10002D) were added and samples were incubated at 4°C for 4 hrs ...
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bioRxiv - Genetics 2020Quote: ... Protein concentration was determined using BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Protein immunoprecipitation was performed using Dynabeads™ Protein G for Immunoprecipitation (Invitrogen) as per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D). 30 µL of beads were used per IP ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by the BCA protein assay kit (Thermo Fisher). The protein solution was aliquoted into a small volume ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Thermo Fisher Scientific). The lysates were fractionated with SDS– PAGE ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Thermo Fisher Scientific). Then ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined with BCA protein assay (Thermo Fisher Scientific, 23225), and equal protein amounts were used in denaturing gel electrophoresis.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher). SDS-PAGE was used to measure beta glucocerebrosidase (GCase ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was measured using Coomassie Plus Protein Assay Reagent (Thermo scientific) and equal amounts of protein were loaded onto 6% SDS gels for anti-V5 and anti-TIM blots or 8% SDS gels for anti-CLK blots respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate protein concentration was measured with BCA Protein Assay (Thermo Fisher 23252). Lysate was diluted to 4 mg/ml in Sup6-150 ...
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bioRxiv - Molecular Biology 2021Quote: Protein content was determined using the Pierce BCA protein assay (Thermo Fisher). 4-10 μg of total protein in Laemmli sample buffer was resolved by SDS-PAGE on 9% polyacrylamide gels and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were measured by QuBit Protein Assay Kit (Thermo Fisher, Q33211) and sarkosyl buffer (50mM HEPES pH 7.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration was measured with Qubit Protein Assay Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentrations were determined using BCA Protein assay Kit (Thermo Scientific Pierce). For immunoprecipitation ...