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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Recombinant Mouse Thpo His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
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bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.
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bioRxiv - Immunology 2021Quote: ... with 10% FBS (Nucleus Biologics, #AU FBS-500ml L1 HI) and 1% Penicillin-Streptomycin (Gibco, #15140122) and transferred to a new tube ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant fraction was incubated with 2 ml Ni-NTA His-Pur resin (Thermo Fisher, PI88222) for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts were released by Bam-HI and Eco-RI digestion and ligated to pENTR4 (Invitrogen; previously digested with the same restriction enzymes) ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The purified His-TEV-FAM19A5 proteins were digested overnight at 30°C with AcTEV protease (Invitrogen) to remove the His-tag and TEV sequence ...
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bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The buffer was then exchanged to the wash solution (10 % Hi-DiTM Formamide (Thermofisher scientific; 4311320) in 2x saline sodium citrate (SSC) ...
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bioRxiv - Molecular Biology 2020Quote: ... these PER2 cDNAs were sub-cloned into various tagged vectors including pcDNA3.0.HA (Thermo Fisher Scientific, Waltham, MA), 3XFLAG-CMV-7.1-2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... FAM fluorescent tagged TaqMan® copy number assay probes detecting the ETNK1 gene (Hs01860263_cn) (Life Technologies, Carlsbad, CA) was used to quantify the copy number of 12p ...
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bioRxiv - Cell Biology 2020Quote: Overexpression of mCherry-tagged actin constructs in HEK293T cells was carried out using Lipofectamine 2000 transfection reagent (ThermoFisher) at a ratio of 1:2.5 ...
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bioRxiv - Bioengineering 2021Quote: ... The Arabidopsis eEF-1 α gene (Assay ID. No. At02337969_g1) tagged with VIC dye (Life Technologies, Carlsbad, CA) was added in each well as an internal control ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged TFs assayed in PBMs were detected either with Alexa488-conjugated anti-GST antibody (Invitrogen A-11131), or with a TF-specific PCRP antibody ...
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bioRxiv - Biophysics 2021Quote: ... 0.2 µg DNA encoding GFP-tagged T-Plastin (WT or mutant) and 0.25 µL Lipofectamine2000 (Thermo Fisher Scientific), mixed in 20 µL OptiMEM (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The expressed C-terminal 6xHis-tagged Nluc was Ni-NTA purified using HisPurTMNi-NTA Resin (ThermoFisher scientific, #88221) as per the HisPurTMNi-NTA Resin protocol ...
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bioRxiv - Cell Biology 2021Quote: ... AF555-and AF647-tagged secondary antibodies (donkey; 1:1000, cat. num. A-21208, A-31572, A-31571, ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: 6-dpf zebrafish larvae were gavaged with Alexa Fluor 488-tagged dextran 10,000MW (fDex) solution (Molecular Probes, USA) or purified mCherry solution (prepared in the laboratory ...
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bioRxiv - Molecular Biology 2022Quote: MCP-tagged SARS-CoV-2 expression plasmids for the MS2 tethering assay were generated by Gateway Assembly (ThermoFisher). SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Biochemistry 2019Quote: ... anti V5-HRP conjugate (to detect mSCARLET and other V5 tagged fluorescent proteins, catalog number 46-0708, Invitrogen), rabbit polyclonal anti TAL1 IgG (catalog number A305-300A ...
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
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bioRxiv - Biochemistry 2021Quote: Flag-tagged UCH37 (WT, C88A, ΔCTD, ΔCTD/C88A, EWI, EWI/C88A) were cloned into pcDNA5/FRT/TO (Invitrogen). Stable cell lines that inducibly express these proteins were constructed from Flp-InTM T-RExTM 293 Cells following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM maltose.) SKD3-MBP fusion protein was cleaved with a 6His-tagged AcTEV™ protease (Thermo Fisher) at 4 °C for 96 h in the amylose elution buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Inserts were then subcloned into pCS2+ N’ HA tagged vectors that had been converted into Gateway® (Invitrogen) destination vectors ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:5000 dilution and endogenous LC3 was tagged with an LC3B/MAP1LC3B primary antibody (ThermoFisher, L10382) used at a dilution of 1:500 for 72 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HA-glmS-tagged parasite lines were coated on diagnostic slides (Epredia; Thermo Fisher Scientific; Waltham, US) and air-dried ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
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bioRxiv - Biochemistry 2022Quote: Cloning of full-length N-terminal strepII-tagged hItln1 into the pcDNA4/myc-HisA vector backbone (Life Technologies) was described previously (14) ...
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bioRxiv - Cell Biology 2023Quote: ... To visualise the cell surface with Alexa488-tagged wheat germ agglutin (Thermo Fisher Scientific, UK, 1:2000 dilution) an incubation was done prior to permeabilization ...
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bioRxiv - Molecular Biology 2023Quote: ... Genes encoding tagged and untagged SOSIP.664 Env homotrimers were expressed by transient transfection of Expi293 cells (ThermoFisher). Env heterotrimers were purified from co-transfections involving a 20:1 expression plasmid DNA ratio of untagged to tagged Env constructs ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged β-catenin variants were expressed in transient transfection assays using the pCDNA3.1 vector (Thermo Fisher Scientific). A plasmid expressing human β-catenin containing a S33Y mutation (pCDNA3-S33Y ...
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bioRxiv - Cell Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5′ ends were synthesized by Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5’ ends were synthesized by Thermo Scientific, while those labeled with ATTO565 at the 5’ end was synthesized by Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: Lamprey embryos were injected with MYC-tagged lamprey (pou5) or Xenopus (pou5f3.1, pou5f3.2, pou5f3.3) mRNA and a FITC dextran tracer (ThermoFisher AAJ6360622) into one blastomere at the two-cell stage at the same concentration used for injections in Xenopus ...
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bioRxiv - Cell Biology 2023Quote: ... strep tagged APC/C was pre-incubated on ice with streptavidin conjugated Alexa Fluor 700 (ThermoFisher Scientific S21383) for 30 minutes ...