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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Recombinant Human CD52 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Immunology 2021Quote: Hela cells stably-expressing EGFP-tagged NLRP3 and mApple-tagged RAB5A were seeded into Nunc™ Lab-Tek™ 8-well Chambered Coverglass (155411, ThermoFisher Scientific) on the day before experiments ...
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bioRxiv - Biochemistry 2020Quote: CHO-K1 cells in 10-cm dishes were transfected with complementary DNA encoding FLAG-tagged mLPCAT2 or one of several FLAG-tagged mLPCAT2 mutants using Lipofectamine 2000 (Thermo Fisher Scientific, USA). At 48 h post-transfection ...
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bioRxiv - Bioengineering 2020Quote: Purified S-ECD protein was biotinylated at a theoretical 1:3 molar ratio with EZ-Link NHS-PEG12-Biotin (Thermo Fisher Scientific, CAT#: 21313) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101, Ovodan Biotech) was expressed in 25 mL culture using the Expi293F expression system (#A14635; ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Developmental Biology 2019Quote: CIL in single cells encountering cadherin coated beads used cdh3-coated beads prepared by binding purified cdh3-fc (gifted from Dr. Douglas W. DeSimone and Dr. Barry. M. Gumbiner) to Protein A/G coated beads (53132, Thermo Fisher Scientific) (Chappuis-Flament et al. ...
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bioRxiv - Microbiology 2022Quote: ... 4a3A cells were maintained at 27°C without CO2 in Insect-XPRESS™ Protein-free Insect Cell Medium complemented with heat-inactivated Fetal Calf Serum (FCS) (#A3840002 ThermoFisher Scientific). C6/36 cell line (ATCC ...
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bioRxiv - Biochemistry 2020Quote: ... Cytosolic extracts were generated as described above and the His6-tagged proteins purified by metal affinity chromatography and gravity flow using 0.5 ml HisPur™ cobalt superflow agarose (Thermo Fisher Scientific™, Waltham, USA) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Immunology 2021Quote: ... or biotinylated anti-IgA antibody (Thermo Scientific, Cat: 7102882500) bound on streptavidin biosensor (Sartorius ...
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bioRxiv - Bioengineering 2022Quote: ... Two microliters of biotinylated anti-EpCAM (Thermo Fisher Scientific) was added ...
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bioRxiv - Genomics 2020Quote: ... Probes were biotinylated with terminal deoxynucleotidyl transferase (TdT, ThermoFisher) and dideoxy-UTP-biotin (biotin-11-ddUTP ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated phalloidin (biotin-XX phalloidin, B7474, Thermo Fisher Scientific), rhodamine-labelled phalloidin (R415 ...
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bioRxiv - Systems Biology 2021Quote: ... and 1 uL of biotinylated y10b antibody (ThermoFisher, MA516060) to each well ...
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bioRxiv - Systems Biology 2020Quote: ... biotinylated VHH-anti EPO (Capture Select™, Thermo Scientific) was immobilized on streptavidin sensors and used to measure EPO binding directly in cell supernatants in citric acid (20 mM) ...
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bioRxiv - Biophysics 2022Quote: ... biotinylated Bovine Serum Albumin (BSA; ThermoFisher; 1 mg/ml) was absorbed to a clean 35mm #1.5 glass-bottom dish (MatTek well ...
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bioRxiv - Molecular Biology 2022Quote: The biotinylated CTD peptides were synthetized by Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 10% biotinylated dextran amine (BDA 10 kD; D1956, Invitrogen) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 10 % biotinylated dextran-amine (BDA; 10,000 MW, Molecular Probes), in the MSDB complex ...
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bioRxiv - Molecular Biology 2022Quote: ... then biotinylated with EZ-link biotin-HPDP (ThermoFisher Scientific) and purified by Streptavidin bead pull-down (Miltenyi) ...
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bioRxiv - Genomics 2020Quote: ... Biotinylated-RNA was separated with M280 Streptavidin Dynabeads (Invitrogen): 25 μl/sample of beads were washed twice with 2 volumes of freshly prepared wash buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... was biotinylated using NHS-PEG4-Biotin (Thermo Fisher, 21330). The protein was de-salted using Zeba Quick Spin columns (Thermo Fisher ...
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bioRxiv - Genetics 2022Quote: ... except the 0.4mM biotinylated dATP (Life Technologies, 19524-016) in the end blunting step was replaced with 10mM unbiotinylated dATP (NEB ...
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bioRxiv - Microbiology 2022Quote: ... Biotinylated RNA was purified using streptavidin M280 Dynabeads (ThermoFisher) after a series of washes ...
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bioRxiv - Biochemistry 2022Quote: ... by incubation with 0.5 mg/mL biotinylated-BSA (ThermoFisher) in Buffer A (25 mM imidazole pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... biotinylated capture antibodies (AT8: Invitrogen, REF MN1020B; HT7: Invitrogen, REF MN1000B ...
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bioRxiv - Cancer Biology 2023Quote: ... Each 1 μg of biotinylated peptide (synthesized by Thermofisher) and 10 μg of bacterially purified GST tagged protein were added and allowed to incubate at 4°C for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... added with biotinylated anti-mouse C1q antibody (Fisher Scientific), and incubated for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... biotinylated RNA was enriched using M280 Streptavidin Dynabeads (Invitrogen) and precipitated using ethanol ...
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bioRxiv - Immunology 2023Quote: ... and horse anti-mouse biotinylated (1:350 Life Technologies). Sections were then incubated in streptavidin-AF594 solution (1:750 μl ...
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bioRxiv - Neuroscience 2024Quote: Bovine serum albumin biotinylated was purchased from Thermo fisher. Cy3-conjugated Human Fc fragment and Biotin-SP-conjugated Goat Anti Human IgG Fc was purchased from Jackson Immunoresearch ...
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bioRxiv - Immunology 2024Quote: ... and biotinylated rat anti-mouse-IL4 (catalogue #13731281, Invitrogen) were used ...