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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for CD25 Mouse Monoclonal Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... using the appropriate antibody (1:10,000; rabbit polyclonal PA1-16889, Thermo Fisher or 1:10,000; mouse monoclonal MA1-140, Thermo Fisher) concomitantly with targets of interest ...
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bioRxiv - Systems Biology 2019Quote: ... along with Pan Cytokeratin (AE1/AE3) (Mouse monoclonal antibody, IgG1, Alexa Fluor® 488 Conjugate; ThermoFisher, cat#53-9003-82), which was diluted to 1:200 in 10% Normal Goat Serum in 1% Bovine Serum Albumin in Phosphate Buffered Saline ...
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bioRxiv - Synthetic Biology 2020Quote: ... The membrane was blocked with 5% skim milk in TBST and probed with HRP-conjugated anti-flag mouse monoclonal antibody (Thermofisher) diluted at 1:10000 in the blocking buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Primary antibody immunolabelling was carried out for 1hr at rt with 1:2000 diluted FLAG tag mouse monoclonal antibody (FG4R, ThermoFisher). After incubation with the secondary alkaline phosphatase-conjugated goat Anti-Mouse IgG (H+L ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated with primary antibodies overnight at 4°C using an antibody against V5 (mouse monoclonal R960-25; 1:1000; Invitrogen), spike (mouse monoclonal GTX632604 ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were incubated for 14–18 h at 37°C and immunostained using a monoclonal anti-βIII tubulin antibody (Tuj1;Covance) and Alexa Fluor 568-conjugated anti-mouse IgG (Invitrogen). For quantification ...
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bioRxiv - Microbiology 2021Quote: ... The attachment of HIV-1 virus produced in the presence of TMEM123 was also separately tested by pre-incubating the virus with mouse monoclonal anti-TMEM123 antibody (297617) (ThermoFisher) (25ug/ml ...
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bioRxiv - Molecular Biology 2022Quote: mLECs were isolated from collagenase I-digested lung tissue using rat anti-mouse CD31 monoclonal antibody-coated Dynabeads (11035, Invitrogen) and directly used for RNA analysis.
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bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
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bioRxiv - Developmental Biology 2022Quote: ... whereas for mouse monoclonal primary antibodies, we used isotype-specific Alexa-conjugated Goat secondary antibodies (against IgG1, IgG2a; IgG2b) from Thermofisher scientific.
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed to detect the actin loading control using a 1:500 dilution of mouse anti-actin monoclonal primary antibody (Cat. No. MA1-744, Lot No. WC317616, Invitrogen) and 1:5000 dilution of HRP-conjugated rabbit anti-mouse secondary antibody (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were generated from monoclonal antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher). Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... Serial sections were processed for immunohistochemistry using the 6F/3D mouse monoclonal amyloid-beta antibody (1:50; ThermoFisher, Waltham, MA) to visualize amyloid plaque pathology.
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bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for one hour at room temperature with a mouse monoclonal anti-E-cadherin (4A2C7, Life Technologies, France) directed against the cytoplasmic domain of E-cad and revealed using an anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... a separate aliquot of each sample during each immunoblot was probed with a monoclonal mouse anti-human GAPDH antibody (1:500 dilution; #43700, Invitrogen) and a peroxidase-conjugated goat anti-mouse secondary antibody (1:5,000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... two mouse monoclonal antibodies were utilized: one for the purpose of IP/ immuno-blotting (Thermo Fisher Scientific catalog # MA5-25915) (Figure 3A and S3A) ...
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bioRxiv - Cell Biology 2022Quote: Commercially available antibodies: mouse monoclonal antibodies to Transferrin Receptor/CD71 (clone H68.4; #13-6890, Thermo Fisher Scientific; western blots (WB) 1:3,000 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an Alexa Fluor® 594 conjugated goat anti-mouse monoclonal antibody at a 1:1000 concentration (A11005; Invitrogen). Neuropilin-1 was visualized using a goat polyclonal anti-NRP-1 antibody at a 15 µg/mL concentration (AF566 ...
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bioRxiv - Immunology 2023Quote: ... Infected cells were then fixed for 20 minutes in 10% formalin and processed for immunofluorescence with 1 μg/mL mouse anti-RhCMV pp65B or 5 ug/mL mouse anti-Rh152/151 monoclonal antibody followed by a 1:500 dilution of goat anti-mouse IgG-Alexa Fluor 488 antibody (Invitrogen). Nuclei were stained with DAPI for 10 minutes (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... The titers of lentiviruses with cell-specific promoters were determined by Western blot for HIV1 p24 band using mouse monoclonal antibody (ThermoFisher).
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bioRxiv - Microbiology 2024Quote: ... Infected cells were then fixed for 20 min in 10% formalin and processed for immunofluorescence with 1 μg/ml mouse anti-RhCMV pp65B or 5 ug/ml mouse anti-Rh152/151 monoclonal antibody followed by a 1:500 dilution of goat anti-mouse IgG-Alexa Fluor 488 antibody (Invitrogen). Nuclei were stained with DAPI for 10 min (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The slides were incubated for 1h at RT with primary antibody (1:20) Mouse Monoclonal Ab anti-AID (ZA001, Invitrogen), in PBS-0.1% BSA-0.4% Tween20 ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated with 5 µg/ml Alexa488- or Alexa568-conjugated transferrin (Tf) (Molecular Probes, T13342 and T23365) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... but also contained a 1:250 dilution of alexa488-conjugated goat antirabbit anitbodies (Fisher Scientific, A-11034), a 1:250 dilution of alex633-conjugated goat anti-mouse antibodies (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... cells were harvested and stained with 100 μg/ml Alexa488-conjugated wheat germ agglutinin (WGA, Molecular Probes) for 35 minutes in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were conjugated to the following fluorophores: Alexa488 (donkey-anti-rabbit, A21206, Molecular Probes, Eugene, USA), CF 488 (donkey-anti-guinea pig ...
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bioRxiv - Bioengineering 2021Quote: ... Subsequently the cells were then incubated with Alexa488 or Alexa647 conjugated secondary antibodies (ThermoFisher Scientific, 1:500) for 1 h at RT ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with anti-H1N1 IAV NP (BEI resources) and Alexa488-conjugated secondary antibody (Life Technologies), while VSV infection rates were measured by detecting virus-encoded GFP ...
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bioRxiv - Biochemistry 2021Quote: ... The endoplasmic reticulum was stained using an Alexa488-coupled anti-calnexin (1:200, mAb AF18, ThermoFisher Scientific) in 10% goat serum overnight at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed in PBS three times and incubated in secondary antibody (alexa488 1:1000, Life technologies) in 1%BSA-1% donkey serum for 1h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with a donkey anti-goat Alexa488-conjugated secondary antibody (A11055, 1:200; Life Technologies, Madison, WI) in PBS with 5% normal donkey serum ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by streptavidin-conjugated Alexa488 for 1 hour at a 1:200 dilution (Thermo Fisher, S-32354). Nuclei were stained using 5 mg/ml DAPI at a 1:1000 dilution for 10 minutes ...
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bioRxiv - Physiology 2022Quote: ... CTb conjugated with Alexa Fluor 488 (Alexa488) or Alexa647 (1 mg/ml, C22841 and C34778, Thermo Fisher) or FG (4% solution dissolved in 0.9% saline ...
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bioRxiv - Microbiology 2022Quote: ... cells were harvested and stained with 100 μg/ml Alexa488-conjugated wheat germ agglutinin (WGA, Molecular Probes) for 35 minutes in the dark ...
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bioRxiv - Microbiology 2023Quote: ... Cells were labelled with Alexa488-labelled wheat germ agglutinin (1 μg/mL, Thermo Fisher Scientific, 60 min), FITC-labelled sambucus nigra lectin (10 μg/mL ...
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bioRxiv - Genetics 2023Quote: ... cells were incubated with secondary antibody (goat anti-rabbit Alexa488, Thermo Fisher Scientific, A-11008, 1:300) for 1 h at room temperature in the dark ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and anti-α-smooth muscle actin Alexa488 (1:200, 53-9760-82, Thermo Fisher Scientific, Massachusetts, USA). Anti-α-smooth muscle actin was added after a series of PBS washes following the first staining ...
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bioRxiv - Developmental Biology 2023Quote: ... Biotin-tagged DNA nicks were visualized using Alexa488-or Alexa647-conjugated streptavidin (Molecular Probes, diluted 1/1000) during the incubation with the secondary antibody.
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3x and secondary staining with alexa488 and alexa647 conjugated antibodies (1:500, Life Technologies) was performed at room temp for 1h together with DNA staining by DAPI (1 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... We used as the secondary an anti-chicken antibody conjugated to Alexa488 from ThermoFisher (Product # A-11039).
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bioRxiv - Immunology 2020Quote: ... cells were labeled with Cell Trace™ Violet and labeled according to the manufacturer’s protocol (Thermofisher). 500,000 total or naïve T cells were then intravenously transferred to congenic CD45.1+ recipients.
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bioRxiv - Immunology 2022Quote: ... T cell proliferation was determined by CFSE dilution and Treg differentiation was determined by surface staining of CD25 and intracellular staining of FOXP3 using the eBioscience Foxp3/Transcription Factor staining buffer set (Invitrogen). Data were collected using a FACSVerse flow cytometer using the BD FACSuite software and were analyzed with FlowJo 10.7.
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bioRxiv - Immunology 2022Quote: ... Responding T cells were isolated from spleens and inguinal lymph nodes of naïve Ly5.1 mice using a pan naïve CD3+ negative selection kit (StemCell) and then FACS sorted (viable, CD3+ CD25-) to achieve >95% purity and labelled with Efluor670 proliferation dye (Invitrogen). Tr1 cells were FACS sorted (viable CD3+CD4+CD44+FOXP3-IL-10+ ...
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bioRxiv - Biophysics 2021Quote: ... Alexa-568-labeled actin (ThermoFisher A12374), and rabbit skeletal myosin II (Cytoskeleton MY02) ...
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bioRxiv - Biophysics 2020Quote: ... and labeled (cy3.5 Invitrogen labeling kit) fibronectin from human plasma (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... AlexaFluor488-or TexasRed-labeled phalloidin (Invitrogen) and DAPI (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... AF488 labeled Zymosan (Invitrogen, cat #: Z23373) was opsonized with FBS from the same lot as used for macrophage media according to manufacturer’s instructions ...