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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 4 4 Bromophenylsulfonyl morpholine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and 4) Alexa Fluor 647 (Cat. No. A21247, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... loaded on NuPAGE 4-12% Bis-Tris Gels (Invitrogen) along with PageRuler Plus Prestained Protein Ladder (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen), and transferred to nitrocellulose membrane with iBlot2 Dry Blotting System (ThermoFisher) ...
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bioRxiv - Biochemistry 2024Quote: ... in 4 mL of OptiPRO SFM media (ThermoFisher Scientific). The solution of DNA-polyethyleneimine was incubated for 10 – 30 minutes at room temperature before being added to the cells ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were fixed in 4% methanol-free formaldehyde (ThermoFisher) for 15 min at room temperature at designated time points during the recovery phase—specifically at untreated ...
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bioRxiv - Cell Biology 2024Quote: ... then fixed in 4% methanol-free formaldehyde (ThermoFisher, 28908) for 45 minutes at 4°C on a shaker to preserve endogenous fluorescent proteins ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained with Fluo-4 AM (Invitrogen, F14201). Samples were rinsed with Hanks’ Buffer (Sigma H6648 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were selected with Puromycin (4 µg/ml, Invitrogen). Successful integration was assessed by genomic PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by fixation with 4% formaldehyde (Thermo Scientific, 28906) containing 10 mM HEPES (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) for 1h at room temperature in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... cells on metasurface were fixed with 4% formaldehyde (Invitrogen) for 15 minutes and washed three times with phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were fixed using 4% paraformaldehyde (ThermoFisher Scientific, 28908) in PBS by perfusion through microfluidic channels or incubating at 37 °C for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... followed by fixation with 4% PFA (Thermo Fisher Scientific). The proteasome was inhibited with 10 µM of MG132 (M7449-200UL ...
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bioRxiv - Developmental Biology 2024Quote: Mouse ovaries were fixed in 4% paraformaldehyde (Thermo Scientific) at 4°C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then fixed in 4% PFA (Thermo Fisher) for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... DAPI (4′,6-diamidino-2-phenylindole, ThermoFisher Scientific 62247) was used to counterstain the nuclei.
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bioRxiv - Genetics 2021Quote: ... the 700 bp PCR products were subcloned into the pCR™4-TOPO® TA vector and transfected into Escherichia coli DH5α cells (TOPO 4 Kit, ThermoFisher Scientific) followed by plasmid preparation and direct Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... pre-stained cells were loaded with Fluo-4 via by mixing with equal volumes of 2X Fluo-4 Direct™ loading solution (Fluo-4 Direct™ Calcium Assay Kit, Thermo Fisher). After a 30 min incubation at 37°C and then 30 mins at RT ...
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bioRxiv - Developmental Biology 2020Quote: Transfection mix was prepared by combining PEI and plasmid DNA (4:1 ratio; 4 µg PEI per 1 µg DNA) in Opti-MEM™ Reduced Serum Medium (Thermo Fisher Scientific) followed by brief vortexing ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then incubated in secondary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Alexa 488 anti-Chicken IgY (Invitrogen A11039; 1:400); Atto 647N anti-mouse IgG (Rockland 610-156-121 ...
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bioRxiv - Microbiology 2022Quote: ... PAFB was labelled with the green fluorophore 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylenediamine hydrochloride (BODIPY™ FL EDA, Invitrogen, Waltham, MA, USA) as described (32).
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bioRxiv - Genetics 2022Quote: ... The styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64, 514/670 nm absorption/emission Invitrogen™, Waltham, Massachusetts) was used at a final concentration of 16.5 μM in ddH2O from a 16.5 mM stock solution in DMSO ...
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bioRxiv - Microbiology 2020Quote: ... 4 and 24 hours after treatment and the plasma membrane was stained with FM 4-64 from Life Technologies (100 μg ml-1). Cells were observed with an epifluorescence Leica DM6000 B research microscope with a 100 x magnification.
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bioRxiv - Pathology 2023Quote: ... Sections were cut (4 µm) using a cryostat and labelled overnight at 4°C with COL6 antibody (1:500) (MA5-32412, Thermo Fisher Scientific, USA) after blocking nonspecific binding sites with 10% bovine serum albumin (A7906 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were quickly fixed overnight (ON) in 1x PBS pH7.4, 4% sucrose (Serva, Heidelberg, Germany) and 4% PFA (Paraformaldehyde; Thermo Fisher Scientific, Waltham, USA). Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, catalogue no. NW04122BOX) and transferred onto a polyvinyl difluoride membrane (Immobilon-FL ...
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bioRxiv - Biophysics 2023Quote: ... cells were transfected with 4 μg of plasmid per 4 million cells using the lipofectamine 2000 reagent and protocol (Thermo Fisher Cat# 11668019). Nuclear extraction was performed 24 hours after transfection utilizing nuclear extraction kit from Abcam (ab113474) ...
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bioRxiv - Bioengineering 2023Quote: ... The staining of MyHC was performed by incubating the samples for 4 hours with the mouse anti-MyHC antibody (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™), which was used at a 1:10 dilution for a final concentration of 10 μg/mL ...
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bioRxiv - Biophysics 2022Quote: ... The grids were automatically blotted for 4 s at 4°C and 100% humidity and flash-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). Cryo-EM data were collected using a 300 kV Titan Krios G2 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... A droplet of 2.5 μL of purified NaV1.6EM complex was applied to the grid followed by blotting for 4-5s at 4°C under 100% humidity using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). In the case of the preparation of NaV1.6EM complex with 4,9-anhydro-TTX ...
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bioRxiv - Cell Biology 2024Quote: ... fixed in 4% PFA for 15 minutes at 4 °C and incubated in 1X InVitrogen eBioscience Cell Permeabilization buffer (InVitrogen 00-8333-56) for 15 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and coverslips were washed with 1X PBS and fixed with cold 4% PFA/PBS pH 7.4 (Paraformaldehyde Solution, 4% in PBS, Thermo Scientific™, #J19943.K2) for 15 minutes at RT ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: We used para-chlorophenylalanine (PCPA, a.k.a. DL-4 Chlorophenylalanine, fenclonine) (CAS: 7424-00-2, ThermoFischer, Acros Organics, Geel, Belgium; stored at 4°C), an inhibitor of the enzyme tryptophan hydroxylase (TPH) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then fixed in 4% formaldehyde (Formaldehyde 37-41% diluted to 4% in ultra-pure water, Fisher Chemical, Thermo Fisher Scientific, USA) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: GUS protein was extracted and GUS activity was quantified as described before 21 by monitoring the quantity of 4-methylumbelliferone products generated from the substrate 4- methylumbelliferyl-b-D-glucuronide (Duchefa) on a fluorometer (Thermo Scientific fluoroskan ascent).
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... using crude extracts from plant leaves and monitoring the quantity of 4-methylumbelliferone products generated from the substrate 4-methylumbelliferyl-β-D-glucuronide (Duchefa) on a fluorometer (Thermo Scientific fluoroskan ascent).
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from gastrocnemius muscles of experimental mice (n=4 BC-PDOX; n=4 BC-PDOX PIO; n=3 NSC-Con) using Trizol (ThermoFisher Scientific, Waltham, MA, USA) and established methods21 ...
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bioRxiv - Bioengineering 2023Quote: ... in 2.5 μl NuPAGE® LDS Sample Buffer (4×) and 1 μl NuPAGE® Sample Reducing Agent (10×)] were loaded in Bolt 4-12% Bis-Tris Plus Gels (Thermo Fisher Scientific, USA). Samples were denatured at 95°C for 3 mins ...
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bioRxiv - Neuroscience 2023Quote: ... Dissected retinas were either fixed in 4% formaldehyde (MilliporeSigma, Cat.# FX0415-4) in PBS for 2 hours at room temperature or lysed with RIPA buffer (Thermo Fisher Scientific, Cat.# 89900) containing protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and brain tissues were weighed and homogenized at a density of 140 mg of dry tissue per milliliter in ethyl acetate / isopropanol (4:1) using a Bead Mill 4 homogenizer (Thermo Fisher Scientific, Waltham MA) and 2-mL pre-filled polypropylene microtubes (2.8 mm ceramic beads ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 4 young (⩽32 years) and 4 older patient (⩾40 years) granulosa cell samples using TRIzol (Life Technologies, Carlsbad, CA, USA) as previously described.111,112 Further ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then incubated in the NGS for at least 4 hrs and incubated overnight at 4°C with secondary antibodies: goat anti-rabbit Alexa Fluor 488 (Invitrogen Cat# A11070; 1/400) and goat anti-mouse HRP-conjugated (Thermo Fisher Scientific Cat# G-21040 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA quantity was measured using a Qubit 4 fluorometer (Qubit 4 fluorometer; Qubit dnDNA HS Assay Kit, Cat No. Q32854 Invitrogen, Eugene, OR, United States). RNA quality was assessed using an Agilent RNA Nano kit on a BioAnalyzer instrument (RNA 6000 Nano Kit ...
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bioRxiv - Cell Biology 2022Quote: ... primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008; 4 μg/ml) in 0.2% BSA for 60 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were postfixed in 4% paraformaldehyde overnight at 4 °C and cut into 50 µm sections on a cryostat microtome (Thermo Fisher, NX50, Waltham, MA). Mounted sections were imaged on a scanning confocal microscope (TCS SP5 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were fixed overnight at 4 °C in 4% PFA freshly diluted from 16% weight/volume PFA aqueous solution (Thermo Fisher Scientific, 043368-9M) in PBS (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... 15 µg total protein per sample was loaded onto a 4-20% or 4-12% linear gradient SDS-PAGE mini-gel (Thermo Fisher Scientific, XP04200BOX, XP04120BOX) and electrophoresed at 150V for 1 hour in 1x running buffer (25 mM tris base ...
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bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 μg/ml of recombinant trimeric S protein (50ul per well ...
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bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 µg/ml of recombinant trimeric S protein (50 µL per well ...
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bioRxiv - Biophysics 2021Quote: ... The AutoScript 4 Software is commercially available from Thermo Fisher Scientific.