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Citations for Thermo Fisher :
1351 - 1400 of 7790 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Alexa Fluor 568-conjugated goat anti-rat (1/1000, ThermoFisher Scientific), or an Alexa Fluor 568-conjugated donkey anti-mouse (1/1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Alexa Fluor 488-conjugated donkey anti-rat (1/1000, ThermoFisher Scientific), Alexa Fluor 568-conjugated goat anti-rat (1/1000 ...
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bioRxiv - Neuroscience 2024Quote: ... rat α-F4/80 (Thermo Fisher Scientific ma1-91124, 1:500), CD68 ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies included donkey-anti-rat-IgG-AlexaFluor405 (1:1000, Invitrogen), donkey-anti-rabbit-IgG-AlexaFluor568 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-rat AlexaFluor 555 (Life Technologies, Cat# A-21434, RRID:AB_2535855), donkey anti-guinea pig AlexaFluor 488 (Jackson Immuno ...
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bioRxiv - Cancer Biology 2024Quote: ... and anti-rat Alexa Fluor 488 (1:500, #A21470, Life Technologies). The incubations were performed in the dark in a humidified chamber ...
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bioRxiv - Bioengineering 2024Quote: ... rat anti-PDGFRA (1:200, 14-1401-82, Invitrogen, RRID: AB_46749), goat anti-ITGA8 (1:20 ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 647 goat anti-rat IgG (Invitrogen, catalog A-21247) at 2 μg/mL and Alexa Fluor 647 goat anti-mouse F(ab’)2 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and Alexa Fluor 647 goat anti-rat IgG (ThermoFisher A-21247). All secondary antibody concentrations used followed the manufacturer’s recommendations.
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bioRxiv - Microbiology 2023Quote: ... fused to a C-terminal C9 tag was generated by sub-cloning the MERS Spike protein from a pLVX-EF1alpha-MERS-Spike plasmid (Weizmann Plasmid Bank) into a pcDNA3.1 expression plasmid using the GeneArt Gibson Assembly HiFi master mix (Thermo Fisher Scientific cat. no. A46627). The following primers were used to generate the vector fragment (Primers V1 ...
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bioRxiv - Genomics 2021Quote: ... The linearized vector was ligated with the annealed shRNA targeting the EWSR1-ETS fusions or with non-targeting control sequence using T4 DNA ligase (Thermo Fisher, Waltham, USA). Stellar competent cells (Clontech ...
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bioRxiv - Cancer Biology 2022Quote: Four oligonucleotide sequences for the miR-NRF2-based shRNAs targeting have been designed using the Block-iT RNAi designer tools (Invitrogen; Supplementary Table 4) and cloned into the pcDNA6.2-GW /EmGFPmiR plasmid according to the manufacturer protocol (BLOCK-iT polII miR RNAi ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmid isolation was performed with the PureLink HiPure Plasmid Filter Maxiprep Kit (Thermo Fisher Scientific). Insertion was checked by DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... The plasmids were isolated using the PureLink® Quick Plasmid Miniprep (Life Technologies Corporation, USA) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and plasmids were isolated from these bacterial cells using GeneJET plasmid miniprep kit (Thermo Fisher). Sequences of CHST-encoding plasmids were verified through Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... with SARS-Cov-2 spike plasmid or a control plasmid using Lipofectamine 2000 (Life technologies) are kind gifts from Olivier Schwartz ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmids containing the gene of interest were extracted using GeneJET Plasmid Miniprep Kit (Thermo Fisher) and confirmed by sequencing.
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bioRxiv - Immunology 2021Quote: ... plasmids were grown under ampicillin selection and purified using GeneJET plasmid Midiprep Kit (Thermo Scientific), following the protocol specified by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and plasmid DNA was extracted using the GeneJET plasmid miniprep kit (Thermo Scientific, Waltham, MA). Plasmid DNA was sent to Eurofins Genomics for sequencing using a plasmid-specific primer included with the CloneJET kit.
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bioRxiv - Cell Biology 2021Quote: ... or a Sf3b1-HRE-luciferase reporter plasmid together with different plasmids using lipofectamine 2000 (Invitrogen). A renilla luciferase plasmid was co-transfected for internal control ...
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bioRxiv - Cell Biology 2020Quote: ... plasmids were purified using a Plasmid DNA Midiprep Kit (K210005, Thermo Fisher Scientific, MA, USA).
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bioRxiv - Physiology 2022Quote: ... The plasmids were then extracted and purified using a PureLink Plasmid Mini Kit (Thermo Fisher) and sequenced (Genetic Analyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant plasmids were isolated by using Gene JET plasmid miniprep kit (Thermo Scientific, USA). The recombinant plasmids of SCD and SREBP1 genes were transfected into the CEH by using the Gene Pulser XcellTM Electroporation system (Biorad) ...
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bioRxiv - Microbiology 2019Quote: ... Plates were scraped and transformant plasmids isolated using the GeneJET plasmid purification kit (Thermo scientific).
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bioRxiv - Synthetic Biology 2020Quote: Yeast plasmid DNA was extracted using the ChargeSwitch Plasmid Yeast Mini Kit (Invitrogen, California, USA) by following the manufacturer’s protocol with minor modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA was extracted from bacteria using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and the obtained concentration was determined by Nanodrop (Life Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... Plasmid isolation was performed with the PureLink HiPure Plasmid Filter Maxiprep Kit (Thermo Fisher Scientific). Insertion was confirmed by DNA sequencing.
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific, Landsmeer, The Netherlands). Oligonucleotide primers (Supplemental Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific, Landsmeer, The Netherlands). PCR products were purified from gel using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNAs used in transfection experiments were purified using the Purelink Plasmid Midi Kit (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... and the plasmids were then extracted using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific). Plasmids were Sanger sequenced on a capillary 3130xL Genetic Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Plasmid DNA was extracted using the GeneJET Plasmid Maxiprep Kit (ThermoFisher Scientific; Cat. No. K0491) for transfections ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pLL3.7m-based plasmids were purified by PureLink Expi Endotoxin-free Maxi plasmid Purification kit (Invitrogen), and then HEK293T (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... The modified pGL4.13 plasmids and pRL-CMV plasmid were co-transfected with Lipofectamine 2000 (Invitrogen) at a mass ratio of 30:1 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were prepared from single colonies using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... with CTL plasmids plus psPAX2 and VSV-G packaging plasmids using Lipofectamine 2000 (Invitrogen 11668019). The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media ...
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bioRxiv - Synthetic Biology 2023Quote: ... Constructed plasmids were extracted using the GeneJET Plasmid Miniprep Kit® (Thermo Fisher Scientific©). All procedures followed the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... by co-transfection of 0.6µg/ml expression plasmid with 0.06µg/ml pCoBLAST selection plasmid (Invitrogen). Cells were grown for 3-5 days before selection with 25µg/ml blasticidin ...
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bioRxiv - Neuroscience 2020Quote: ... because we were not able to identify a specific shRNA target sequence using web-based design tools (InvivoGen siRNA Wizard, and Invitrogen Block-iT RNAi Designer). For calcium imaging ...
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bioRxiv - Biochemistry 2019Quote: NPM1 knockdown cell line was generated in AW13516 cell line by transfecting with NPM1 shRNA construct in pTRIPZ vector (Thermofisher Scientific, Waltham, MA, USA) for a period of 24 h followed by antibiotic selection using 1.6 µg/ml puromycin for 4 days ...
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bioRxiv - Microbiology 2020Quote: ... Maxipreps of rescue plasmids were purified using PureLink™ HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Transfection grade plasmid DNA were isolated using the PureLink HiPure Plasmid Miniprep Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... The plasmid was purified using a maxiprep commercial kit (PureLink HiPure Plasmid Filter Maxiprep Kit, Invitrogen). BV2 cells were seeded in 24 well-plates at 70-90% confluence 24 hours prior to the experiment in high glucose DMEM (Gibco ...
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bioRxiv - Genomics 2019Quote: ... cells were transfected with 40 ng renilla plasmid + 400 ng pGL4.23 plasmid using Lipofectamine 2000 (Invitrogen). 48 hours following transfection ...
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bioRxiv - Plant Biology 2019Quote: ... Cas9 and sgRNA plasmids were extracted with the PureLink HiPure Plasmid Midiprep Kit (Invitrogen, cat#K210005). Single stranded salt-free oligonucleotide templates at the length of 42-62 nt were synthesized and dissolved in sterile water at a final concentration of 50 μM ...
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bioRxiv - Biochemistry 2020Quote: ... and plasmid DNA isolated using a PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit (Thermo Scientific). The purified plasmid was finally digested again with HindIII/SnaBI and gel-purified ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmids were harvested from the cultures using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen™). After extraction of the assembled plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected with plasmids (pCAGGS-EGFP and the px333 plasmids) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids used for injection were purified using the PureLink HQ Mini Plasmid DNA Purification Kit (ThermoFisher) using the extra wash step and buffer recommended for endA+ strains ...
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bioRxiv - Cell Biology 2021Quote: ... packaging plasmid psPAX2 (3μg) and envelope plasmid pMD2.G (3μg) using Lipofectamine 2000 Transfection Reagent (Invitrogen). Post 18 hr ...