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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Phospho p95 NBS1 S343 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Fe-NTA enrichment was carried out with a High Select Fe-NTA phospho-peptide enrichment kit (Thermo Fisher Scientific), as previously described29,30 ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used for the signaling pathways: phospho-Src (Tyr418, clone SC1T2M3; 1:10) from Thermo Fisher, mouse IgG2b isotype control from Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... and rabbit anti-GFP (polyclonal anti-GFP rabbit IgG, A11122 Invitrogen, 1:000 ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit antibodies were coupled to magnetic beads conjugated to anti-rabbit IgG (Dynabeads Sheep anti-rabbit IgG, Invitrogen, #11203D). The buffer containing 200 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... hybridoma cells were adapted to and expanded in serum free medium containing 1:2 vol/vol Cell™ MAB medium (Life Technologies) and HyClone™ CDM4MAB medium (GE Life Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems) in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Microbiology 2022Quote: ... recovered sporozoites cells were stained with anti-PvCSP mAbs 1:500 and fixed 10 min in 100uL of a 2% PFA solution (Thermo Scientific™) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... They were stained for 2 days at 4°C with a mouse-derived antibody against GFP (1:400, mAB 3E6, Invitrogen, Darmstadt, Germany), washed for 2 hours at RT in 1x PBS ...
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bioRxiv - Immunology 2022Quote: The binding capacity of the glycoengineered COVA1-18 (2C1) hIgG1 mAbs was tested by directly coating Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific) O/N at 4°C with 100 µL 1 µg/mL purified SARS-CoV-2 RBD-protein ...
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bioRxiv - Immunology 2022Quote: ... OEP light cages were applied to export individual cross-reactive mAb secreting B cells directly into Dynabeads mRNA DIRECT lysis buffer (Life Technologies, 61011) in 96-well plates ...
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bioRxiv - Microbiology 2022Quote: ... The internalization of the ACE2-HA protein and anti-hACE2 mAbs was then evaluated by staining with goat anti-mouse Alexa Fluor™ 594 (to detect the HA-tagged hACE2) and/or goat anti-human Alexa Fluor™ 488 antibodies (to detect the hACE2 mAbs) (ThermoFisher Scientific). Images were captured using a DeltaVision OMX SR imaging system (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... the unbound xfR5 mAb was washed off by dialysis using Float-A-Lyzer™ G2 Dialysis Devices (Thermo Fisher Scientific; NH, USA) in cold PBS supplemented with 10mM hydroxylamine to quench any non-reacted NHS groups present on the xfR5 mAb conjugated D+T NPs (xfR5-D+T NPs ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... and unlabeled anti-LTβR mAb and subsequently counterstained with streptavidin-Alexa 488 and goat-anti-rat-Alexa 488 (all Invitrogen, Breda, The Netherlands), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Permeabilized cells were probed with a monoclonal antibody against the HA-tag epitope (1:1000; HA Tag mAb 2-2.2.14, Thermo Fisher Scientific, Planegg, Germany) to detect SARS-2-S protein ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once before being stained with surface mAb (Table S1) and viability dye LIVE/DEAD Fixable Near-IR (Thermo-Fisher Scientific, USA) for 30 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: Monoclonal antibodies (mAbs) targeting β-GlcNAc-WTA (clone 4497) in different Ig isotypes and IgG subclasses were expressed in EXPI293F cells (Thermo Fisher) as previously described (29 ...
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bioRxiv - Immunology 2023Quote: ... anti-SIVmac251 Nef mAb (clone 17, epitope peptide corresponding to SIVmac239 Nef 71-80 not including the residue G63: Thermo Scientific/Pierce) manually conjugated with Alexa 488 or Alexa 647 ...
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bioRxiv - Immunology 2023Quote: ... they were resuspended in 0.5% BSA/PBS for prevention of experimental procedure-related loss and stained with mouse anti-SIVmac251 Nef mAb (clone 17, Thermo Scientific/Pierce) or mouse IgG1 isotype control mAb (P3.6.2.8.1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated in the dark for 30-45 min on ice with goat anti-mouse fluorochrome conjugated isotype specific mAbs (Life Technologies Corporation, Invitrogen, USA), washed twice and re-suspended in 2% BSA PBS ...
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bioRxiv - Immunology 2023Quote: ... were coated with 10 µg/mL of anti-LAIR-1 agonist mAb (clone Dx26) or 10 µg/mL of mouse isotype control IgG1 (eBioscience-Thermo Fisher Scientific) diluted in PBS overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... rabbit monoclonal antibody against Ki67 (Rabbit, RM-9106-S0; Thermo Fisher Scientific) and caspase-3 (Mouse ...
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bioRxiv - Plant Biology 2021Quote: ... Rabbit polyclonal anti-AtTET8 (Homemade) and normal rabbit immunoglobulin G (Thermo Fisher), were coated with protein A beads in IP buffer (20 mM MES hydrate ...
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bioRxiv - Immunology 2022Quote: ... or irrelevant rabbit IgG (anti-green fluorescence protein of rabbit IgG, ThermoFisher), or each of the mouse monoclonal or rabbit polyclonal CspZ-YA IgGs (0.4 nM ...
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bioRxiv - Neuroscience 2020Quote: ... was coupled to rabbit IgG and rabbit anti-FEZ1 antibody (ThermoFisher PA590412) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... α-Sse1 (rabbit 1:100000) and α-GFP (rabbit 1:5000, Invitrogen #A6455). α-Pgk1 (mouse 1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were then incubated with primary antibody (anti-4HNE from Millipore 1:1000; anti-DNP 1:500; anti-phospho-NFκB from Invitrogen 1:1000; anti-NFκB from Invitrogen 1:1000) at 4°C overnight ...
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bioRxiv - Plant Biology 2020Quote: 10 DAG seedlings were used for western blotting to detect the endogenous level of the phosphorylated (Phospho-p70 S6 kinase pThr389 antibody; dilution: 1:1000; catalog no.: MA5-15117, Invitrogen) and total (Ribosomal S6 kinase 1/2 antibody ...
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bioRxiv - Cell Biology 2021Quote: ... The autophosphorylation of hIRE1 KR was confirmed by western blot using a pSer/24 phospho-specific antibody (Genentech) and phosphorylated Pumilio proteins were detected using anti-mouse-V5 antibody (Invitrogen). In the case of kinase radioactive assays ...
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bioRxiv - Plant Biology 2022Quote: ... and mCherry/pDONRP2rP3 into pK7m34GW (phospho-mutants) or pH7m34GW (truncations and DPF deletion) expression vectors (Karimi et al., 2002) by LR Clonase II (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... Antibody was affinity purified on phospho-peptide covalently bound to a SulfoLink column according to the manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Plant Biology 2021Quote: ... and the resulting pDONR221-RBR_N*AB*C* phospho-variants entry clones were recombined into pDEST32 by Gateway LR clonase II enzyme mix (Invitrogen, 11791020), resulting in the bait plasmids (pEXP32-RBR_N*AB*C* phospho-variants).
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bioRxiv - Pathology 2022Quote: ... and phosphorylated paired helical filament tau with phospho-Ser202+Thr205 using a monoclonal antibody (AT8, 1:1,000; MN1020, ThermoFisher [47]). Endogenous peroxidase was quenched by 20 min incubation in 0.1 M sodium periodate ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated in primary antibody diluted in blocking buffer using the following primary antibodies: anti-Phospho-Tau (Ser396) (1:500, 44-752G, Invitrogen), anti β-catenin (E-5 ...
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bioRxiv - Neuroscience 2023Quote: ... and blocked with 3% normal goat serum in Tris-buffered saline for immunostaining with the phospho-tau (Ser202, Thr205) AT8 antibody (1:200, MN1020, Invitrogen).
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bioRxiv - Neuroscience 2023Quote: ... the immediate adjacent sections (4-µm thickness) were stained against phospho-tau AT8 (pS202/pT205, 1:1,000 dilution, Thermo Scientific) using the Autostainer Link 48 (Dako) ...
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bioRxiv - Cell Biology 2024Quote: ... and phospho-peptides were subjected to LC-MS/MS analysis using an Orbitrap QE LC-MS/MS system (Thermo Scientific) at the proteomics core facility of UT Southwestern Medical Center ...
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bioRxiv - Biochemistry 2024Quote: ... To separate phospho from non-phospho LRRKtide the following HPLC protocol was used: Hypersil Gold C18 (2.1×150 mm; 3 µm particle size, Thermo Scientific), Buffer A ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel droplets were overlaid with recombinant organoid medium (Advanced DMEM/F-12 supplemented with 1X B27 (Gibco), 1X N2 (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and 50μl Pierce recombinant protein A/G with peroxidase (Thermo Fisher catalog no: 32490) added at 1:10,000 in PBS-T with 1% milk as a secondary and incubated 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: Clones of pFastBac1 constructs were used to generate recombinant bacmids in DH10Bac following the manufacturers protocol (ThermoFisher). Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus) ...
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bioRxiv - Genomics 2020Quote: ... cells were serum-starved (with 0.3% SCS) for 72 hours prior to induction of gene expression with 0.1µg/mL recombinant EGF (Gibco) or PBS for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: Recombinant PfbA (rPfbA) or BSA was coated onto 0.5-µm-diameter fluorescent beads (FluoroSphere, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 ng of poly(dI-dC) were used for 100 ng of recombinant ERα (ThermoFisher, RP-310) and 1 µg for 10 µg of nuclear extracts ...