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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for PCNA Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Immunology 2022Quote: ... Recombinant baculoviruses were produced using the Bac-to-Bac baculovirus expression system according to the manufacturer’s manual (Invitrogen).The scheme for constructing the recombinant baculoviruses ...
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bioRxiv - Microbiology 2022Quote: ... Bacmids and recombinant baculoviruses were generated from the pFastBac Dual vectors using the Bac-to-Bac system (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... Triturated tissue was then digested for 5 min using the recombinant protease TrypLE Express (ThermoFisher Scientific, cat. #12605) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Microbiology 2024Quote: The plasmids for the recombinant SARS-CoV-2 variants were transiently transfected into Expi293F cells (Thermo Fisher Scientific) with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB10 with DNA isolated from recombinant strains (via the Whole Blood Genomic DNA Purification Kit, Thermo Scientific) followed by selection on LB agar with the appropriate drug ...
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bioRxiv - Genetics 2024Quote: ... The recombinant plasmids were transfected into 293T cells using LipofectamineTM 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA). For RT-qPCR ...
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bioRxiv - Immunology 2023Quote: ... was made by mixing 2.5ug of IL-2 monoclonal antibody (JES6-1A12, 2B Scientific) with 7.5ug recombinant IL-2 (ThermoFisher) in 200ul of PBS for 20 minutes at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... and the concentration of the recombinant plasmids was determined using the Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851). 293FT cells were transfected with 1 µg of recombinant plasmids or blank vectors with 40 ng of pRL-TK Renilla luciferase control vector using Lipofiter ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 0.134% Triton X-100 [Sigma], 0.5 U/µL recombinant RNase inhibitor [Takara, 2313B], 3.33 mM each dNTP [ThermoFisher, R1121] ...
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bioRxiv - Genomics 2023Quote: ... residual RNA contamination was removed by incubating the samples in 10µL of 1mg/mL recombinant RNase (Ambion, #AM2269). Sample aliquots of 100µL were kept at -80 °C.
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Evolutionary Biology 2023Quote: ... secondary antibody (goat anti-mouse IgG H + L superclonal recombinant secondary antibody with Alexa Fluor 488; Invitrogen #A28175) 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with a mixture of ketamine/xylazine and then infected via retroorbital injection of 100µl containing 1×105 genome equivalents (G.E.) of recombinant NrHV diluted in DPBS (Gibco) (N = 5/strain) ...
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bioRxiv - Bioengineering 2023Quote: ... The recombinant antibodies were transiently expressed in both Expi CHO and Expi293 cells according to manufacturer’s protocol (Invitrogen). The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... alanine derivatives were used in a concentration of 10 mM and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies and recombinant RBD-hFc proteins were purified using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424). In general ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant baculoviruses were prepared in the Spodoptera frugiperda (sf9) cells using the Bac-to-Bac system (Life Technologies). HIF2α and BMAL1 were co-expressed in Trichoplusia ni Hive Five cells by infection of 25 ml each of baculoviruses per 1 L of High Five culture ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was in vitro transcribed from the linearized recombinant infectious clone using the mMESSAGE mMACHINE Kit (Ambion) and electroporated into Vero E6 cells ...
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bioRxiv - Molecular Biology 2021Quote: Hybridoma cell lines for mAbs 2F2 and 2E10.E9 (BEI Resources MRA-184 and MRA-185, respectively) were cultured in GIBCO Hybridoma-SFM (Thermo Fisher Scientific Cat#12045076) with 5–20% fetal bovine serum (Thermo Fisher Scientific Cat#12483–020) ...
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bioRxiv - Systems Biology 2021Quote: ... the mAb samples were affinity-purified from the cell culture supernatant with a 96-well Protein G IgG purification plate (Thermo Fisher Scientific, Dublin, Ireland). Glycans were released from the mAb through PNGase (Prozyme ...
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bioRxiv - Immunology 2022Quote: ... chabaudi and then treated with four doses of (0.5mg/mouse) of depleting mAbs against IFN-γ or isotype control antibody (H22; Thermo Fisher Scientific, Waltham, MA) or (15 ng/mouse ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... Cell culture supernatants containing specific anti-N mAb were harvested and concentrated using Pierce™ Saturated Ammonium Sulfate Solution (Thermo Fisher Scientific, Waltham, MA). Biotinylation of the mAb was performed using a Biotin Conjugation Kit by following the manufacturer’s instruction (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated in the dark for 30-45 min on ice with goat anti-mouse fluorochrome conjugated isotype specific mAbs (Life Technologies Corporation, Invitrogen, USA), washed twice and re-suspended in 2% BSA PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-rabbit or -mouse Alexa 488 and anti-mouse or -rabbit Alexa 568 (Invitrogen) secondary antibodies were used at a 1:250 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 488) or anti-rabbit antibody (Thermofisher, Goat anti-Rabbit IgG (H+L) Secondary Antibody ...
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bioRxiv - Physiology 2019Quote: ... anti-rabbit (Alexa Fluor® 488 Donkey anti-rabbit, Thermo Fisher A21206; 1:1000) and DAPI (Hoechst 33342 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-Rabbit Alexa 488 and anti-Rabbit Cy3 (1:200) procured from Molecular probes, USA.
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bioRxiv - Developmental Biology 2023Quote: ... pH 7.6 (Rabbit anti-HA, Cell Signaling, 37245; rabbit anti-Podocin, Invitrogen, PA5-79757). Sections were incubated overnight at 4°C followed by 4×10 min washes in TBS/BSA-Ac to remove unbound antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-rabbit secondary antibodies (goat anti-rabbit Alexa Fluor 594; 1:2000; Invitrogen). Duolink Mounting Medium with DAPI was used to mount the coverslips ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-rabbit 800 (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-GFP (A11122; Invitrogen), rabbit anti-PARP1 (9542 ...
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bioRxiv - Cell Biology 2020Quote: ... sheep anti-rabbit-HRP (Invitrogen), or goat anti-rabbit or goat anti-mouse IRdye 680 (1:20.000 ...
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bioRxiv - Immunology 2021Quote: ... and α-rabbit AF488 (Invitrogen) nucleus were labelled by Hoechst.
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bioRxiv - Immunology 2021Quote: ... goat anti-rabbit IgG (Invitrogen).
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bioRxiv - Genomics 2022Quote: ... goat anti-rabbit (Invitrogen #G21234).
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal anti-VGAT (Invitrogen, cat ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-GFP rabbit antibody (Invitrogen) was used at 1:250 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-GFP (A11122, Invitrogen) followed by horseradish peroxidase-coupled antibodies (Goat anti-mouse ...
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bioRxiv - Cell Biology 2022Quote: ... donkey anti-rabbit (Invitrogen, A21206), goat anti-mouse IgG2a (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-HA (Invitrogen, 715500), mouse anti-Myc (Abmart ...