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1351 - 1400 of 10000+ citations for CD23 Rabbit Recombinant mAb since 2019

• Machine Learning-Guided Antibody Engineering That Leverages Domain Knowledge To Overcome The Small Data Problem

  Thomas Clark, et al., bioRxiv - Bioengineering 2023

  Quote: ... The recombinant antibodies were transiently expressed in both Expi CHO and Expi293 cells according to manufacturer’s protocol (Invitrogen). The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies) ...
• Identification and in vitro characterization of UDP-GlcNAc-RNA cap-modifying and decapping enzymes

  Frederik Weber, et al., bioRxiv - Biochemistry 2023

  Quote: ... alanine derivatives were used in a concentration of 10 mM and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
• Functional and long-lived melanocytes from human pluripotent stem cells with transient ectopic expression of JMJD3

  Chie Kobori, et al., bioRxiv - Cell Biology 2023

  Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
• Rapid evolution of the embryonically-expressed homeobox gene LEUTX within primates

  Thomas D. Lewin, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... secondary antibody (goat anti-mouse IgG H + L superclonal recombinant secondary antibody with Alexa Fluor 488; Invitrogen #A28175) 1:1000 ...
• Host genetic variation guides hepacivirus clearance, chronicity, and liver fibrosis in mice

  Ariane J. Brown, et al., bioRxiv - Microbiology 2023

  Quote: ... Mice were anesthetized with a mixture of ketamine/xylazine and then infected via retroorbital injection of 100µl containing 1×105 genome equivalents (G.E.) of recombinant NrHV diluted in DPBS (Gibco) (N = 5/strain) ...
• Evidence of antigenic drift in the fusion machinery core of SARS-CoV-2 spike

  Timothy J.C. Tan, et al., bioRxiv - Microbiology 2023

  Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
• Endothelial-secreted Endocan protein acts as a PDGFR alpha ligand and regulates vascularity, radioresistance, and regional phenotype in glioblastoma

  Soniya Bastola, et al., bioRxiv - Cancer Biology 2023

  Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
• Impaired central pattern generators due to abnormal EPHA4 signaling leads to idiopathic scoliosis

  Lianlei Wang, et al., bioRxiv - Genetics 2024

  Quote: ... The recombinant plasmids were transfected into 293T cells using LipofectamineTM 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA). For RT-qPCR ...
• Multiple independent acquisitions of ACE2 usage in MERS-related coronaviruses

  Cheng-Bao Ma, et al., bioRxiv - Microbiology 2024

  Quote: ... Antibodies and recombinant RBD-hFc proteins were purified using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424). In general ...
• BMAL1-HIF2α heterodimers contribute to ccRCC

  Rebecca M. Mello, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Recombinant baculoviruses were prepared in the Spodoptera frugiperda (sf9) cells using the Bac-to-Bac system (Life Technologies). HIF2α and BMAL1 were co-expressed in Trichoplusia ni Hive Five cells by infection of 25 ml each of baculoviruses per 1 L of High Five culture ...
• Midline Assembloids Reveal Regulators of Human Axon Guidance

  Massimo M. Onesto, et al., bioRxiv - Neuroscience 2024

  Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
• A recombinant CHIKV-NLuc virus identifies chondrocytes as target of Chikungunya virus in a immunocompetent mouse model

  Vincent Legros, et al., bioRxiv - Microbiology 2024

  Quote: ... Viral RNA was in vitro transcribed from the linearized recombinant infectious clone using the mMESSAGE mMACHINE Kit (Ambion) and electroporated into Vero E6 cells ...
• Structural basis of Plasmodium vivax inhibition by antibodies binding to the circumsporozoite protein repeats

  Iga Kucharska, et al., bioRxiv - Molecular Biology 2021

  Quote: Hybridoma cell lines for mAbs 2F2 and 2E10.E9 (BEI Resources MRA-184 and MRA-185, respectively) were cultured in GIBCO Hybridoma-SFM (Thermo Fisher Scientific Cat#12045076) with 5–20% fetal bovine serum (Thermo Fisher Scientific Cat#12483–020) ...
• CHOmpact: a reduced metabolic model of Chinese hamster ovary cells with enhanced interpretability

  Ioscani Jiménez del Val, et al., bioRxiv - Systems Biology 2021

  Quote: ... the mAb samples were affinity-purified from the cell culture supernatant with a 96-well Protein G IgG purification plate (Thermo Fisher Scientific, Dublin, Ireland). Glycans were released from the mAb through PNGase (Prozyme ...
• Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animals

  Fangfeng Yuan, et al., bioRxiv - Microbiology 2023

  Quote: ... Cell culture supernatants containing specific anti-N mAb were harvested and concentrated using Pierce™ Saturated Ammonium Sulfate Solution (Thermo Fisher Scientific, Waltham, MA). Biotinylation of the mAb was performed using a Biotin Conjugation Kit by following the manufacturer’s instruction (Abcam ...
• Effects of Low-level Persistent Infection on maintenance of immunity by CD4 T cell subsets and Th1 cytokines

  Samad A. Ibitokou, et al., bioRxiv - Immunology 2022

  Quote: ... chabaudi and then treated with four doses of (0.5mg/mouse) of depleting mAbs against IFN-γ or isotype control antibody (H22; Thermo Fisher Scientific, Waltham, MA) or (15 ng/mouse ...
• Preferential differential gene expression within the WC1.1⁺ γδ T cell compartment in cattle naturally infected with Mycobacterium bovis

  Sajad A. Bhat, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were then incubated in the dark for 30-45 min on ice with goat anti-mouse fluorochrome conjugated isotype specific mAbs (Life Technologies Corporation, Invitrogen, USA), washed twice and re-suspended in 2% BSA PBS ...
• Intramolecular regulation of anillin during cytokinesis

  Daniel Beaudet, et al., bioRxiv - Cell Biology 2019

  Quote: ... Anti-rabbit or -mouse Alexa 488 and anti-mouse or -rabbit Alexa 568 (Invitrogen) secondary antibodies were used at a 1:250 dilution ...
• Enhanced Neuronal Activity and Asynchronous Calcium Transients Revealed in a 3D Organoid Model of Alzheimer’s Disease

  Juan Yin, Antonius M. VanDongen, bioRxiv - Neuroscience 2020

  Quote: ... Alexa Fluor 488) or anti-rabbit antibody (Thermofisher, Goat anti-Rabbit IgG (H+L) Secondary Antibody ...
• Multiple human adipocyte subtypes and mechanisms of their development

  So Yun Min, et al., bioRxiv - Physiology 2019

  Quote: ... anti-rabbit (Alexa Fluor® 488 Donkey anti-rabbit, Thermo Fisher A21206; 1:1000) and DAPI (Hoechst 33342 ...
• Age dependent trans-cellular propagation of human tau aggregates in Drosophila disease models

  Aqsa, Surajit Sarkar, bioRxiv - Neuroscience 2020

  Quote: ... anti-Rabbit Alexa 488 and anti-Rabbit Cy3 (1:200) procured from Molecular probes, USA.
• The cholesterol biosynthesis enzyme FAXDC2 couples Wnt/β-catenin to RTK/MAPK signaling

  Babita Madan, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and anti-rabbit secondary antibodies (goat anti-rabbit Alexa Fluor 594; 1:2000; Invitrogen). Duolink Mounting Medium with DAPI was used to mount the coverslips ...
• Mapping of the podocin proximity-dependent proteome reveals novel components of the kidney podocyte foot process

  Gary F. Gerlach, et al., bioRxiv - Developmental Biology 2023

  Quote: ... pH 7.6 (Rabbit anti-HA, Cell Signaling, 37245; rabbit anti-Podocin, Invitrogen, PA5-79757). Sections were incubated overnight at 4°C followed by 4×10 min washes in TBS/BSA-Ac to remove unbound antibody ...
• Mammalian copper homeostasis requires retromer-dependent recycling of the high-affinity copper transporter 1 (CTR1/SLC31A1)

  Rachel Curnock, Peter J. Cullen, bioRxiv - Cell Biology 2020

  Quote: ... goat anti-rabbit 800 (Invitrogen).
• Poly(ADP-ribose)-binding and macroH2A mediate recruitment and functions of KDM5A at DNA lesions

  Ramhari Kumbhar, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti-GFP (A11122; Invitrogen), rabbit anti-PARP1 (9542 ...
• ER-embedded UBE2J1/RNF26 ubiquitylation complex in spatiotemporal control of the endolysosomal pathway

  Tom Cremer, et al., bioRxiv - Cell Biology 2020

  Quote: ... sheep anti-rabbit-HRP (Invitrogen), or goat anti-rabbit or goat anti-mouse IRdye 680 (1:20.000 ...
• SiglecF^high neutrophils in lung tumor tissues suppress local CD8 T cell responses and limit the efficacy of anti PD-L1 antibodies

  Francesca Simoncello, et al., bioRxiv - Immunology 2021

  Quote: ... and α-rabbit AF488 (Invitrogen) nucleus were labelled by Hoechst.
• Potent but transient immunosuppression of T-cells is a general feature of CD71⁺ erythroid cells

  Tomasz M. Grzywa, et al., bioRxiv - Immunology 2021

  Quote: ... goat anti-rabbit IgG (Invitrogen).
• In vitro evolution and whole genome analysis to study chemotherapy drug resistance in haploid human cells

  Juan Carlos Jado, et al., bioRxiv - Genomics 2022

  Quote: ... goat anti-rabbit (Invitrogen #G21234).
• Late adolescence mortality in mice with brain-specific deletion of the volume-regulated anion channel subunit LRRC8A

  Corinne S. Wilson, et al., bioRxiv - Neuroscience 2021

  Quote: ... rabbit polyclonal anti-VGAT (Invitrogen, cat ...
• Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation

  Mohammad Zeeshan, et al., bioRxiv - Cell Biology 2020

  Quote: ... Anti-GFP rabbit antibody (Invitrogen) was used at 1:250 dilution ...
• Regulated control of gene therapies with a drug induced switch

  Alex Mas Monteys, et al., bioRxiv - Molecular Biology 2020

  Quote: ... rabbit anti-GFP (A11122, Invitrogen) followed by horseradish peroxidase-coupled antibodies (Goat anti-mouse ...
• Stress-induced phase separation of ERES components into Sec bodies precedes ER exit inhibition in mammalian cells

  Wessel van Leeuwen, et al., bioRxiv - Cell Biology 2022

  Quote: ... donkey anti-rabbit (Invitrogen, A21206), goat anti-mouse IgG2a (Invitrogen ...
• A molecular brake that modulates spliceosome pausing at detained introns contributes to neurodegeneration

  Dawei Meng, et al., bioRxiv - Cell Biology 2022

  Quote: ... rabbit anti-HA (Invitrogen, 715500), mouse anti-Myc (Abmart ...
• ATF4 mediates fetal globin upregulation in response to reduced β-globin

  Mandy Boontanrart, et al., bioRxiv - Genetics 2020

  Quote: ... rabbit anti-LRF (ThermoFisher PA528144) 1:1000 ...
• Genome-wide assessment of REST binding profiles reveals distinctions between human and mouse hippocampus

  James C. McGann, et al., bioRxiv - Genomics 2020

  Quote: ... rabbit anti-HDAC2 (Life Technologies, RRID:AB_2547079 ...
• Mps1 releases Mad1 from nuclear pores to ensure a robust mitotic checkpoint and accurate chromosome segregation

  Mariana Osswald, et al., bioRxiv - Cell Biology 2019

  Quote: ... rabbit anti-GFP (Molecular Probes) used at 1:5000 for Drosophila intestines ...
• Robo2 regulates synaptic oxytocin content by affecting actin state

  Savani Anbalagan, et al., bioRxiv - Developmental Biology 2019

  Quote: ... Rabbit anti-EGFP (ThermoFisher A11122), Guinea Pig anti-OXT (Peninsula labs T-5021 ...
• Infection-driven activation of transglutaminase 2 boosts glucose uptake and hexosamine biosynthesis

  Benoit Maffei, et al., bioRxiv - Microbiology 2019

  Quote: ... or anti-rabbit-HRP (Invitrogen) conjugated antibodies ...
• Neuroinflammation and EIF2 signaling persist in an HiPSC tri-culture model of HIV infection despite antiretroviral treatment

  Sean K. Ryan, et al., bioRxiv - Pathology 2019

  Quote: ... Rabbit anti-CCR5 (Thermo Fisher Scientific pa5-29011 ...
• SETD2 is an actin lysine methyltransferase

  Riyad N.H. Seervai, et al., bioRxiv - Cell Biology 2020

  Quote: ... SETD2 (rabbit, Invitrogen, PA5-83615), SETD2 (rabbit ...
• The mechanisms of dynamin-actin interaction

  Ruihui Zhang, et al., bioRxiv - Cell Biology 2019

  Quote: ... anti-rabbit-HRP (1;10,000; Invitrogen), anti-mouse-HRP (1;10,000 ...
• Multiple Sex-Specific Differences in the Regulation of Meiotic Progression in C. elegans

  Sara M. Fielder, Rieke Kempfer, William G. Kelly, bioRxiv - Genetics 2020

  Quote: ... donkey anti-rabbit (Invitrogen A21207), donkey anti-mouse (Invitrogen A21203) ...
• Enhanced lipogenesis through Pparγ helps cavefish adapt to food scarcity

  Shaolei Xiong, et al., bioRxiv - Evolutionary Biology 2021

  Quote: ... goat anti-rabbit (Invitrogen, A32733), and donkey anti-mouse (Invitrogen ...
• Overexpression of Reticulon 3 enhances CNS axon regeneration and functional recovery after injury

  Sharif Alhajlah, Adam M. Thompson, Zubair Ahmed, bioRxiv - Neuroscience 2021

  Quote: ... Alexa488 anti-rabbit (#A323731, Invitrogen)/Texas Red anti-mouse (#PA1-28626 ...
• JAK1 palmitoylation by ZDHHC3/7 is Essential for Neuropoietic Cytokine Signaling and DRG Neuron Survival

  Luiselys M. Hernandez, et al., bioRxiv - Neuroscience 2020

  Quote: ... rabbit anti-GFP #A11122 (Invitrogen); mouse anti-JAK1 #610231 (BD Transduction Labs) ...
• Selective increase of functional network connectivity in Arc-positive neuronal engrams after long-term potentiation

  Yuheng Jiang, Antonius M.J. VanDongen, bioRxiv - Neuroscience 2020

  Quote: ... Rabbit-anti-NeuN (Thermo Fisher Scientific Cat# 711054 ...
• Tumor expressed CD95 causes suppression of anti-tumor activity of NK cells in a model of triple negative breast cancer

  Abdul S. Qadir, et al., bioRxiv - Cancer Biology 2021

  Quote: ... or polyclonal rabbit IgG (ThermoFisher) both in 100 μl ...
• ORP5 AND ORP8 ORCHESTRATE LIPID DROPLET BIOGENESIS AND MAINTENANCE AT ER-MITOCHONDRIA CONTACT SITES

  Valentin Guyard, et al., bioRxiv - Cell Biology 2021

  Quote: ... rabbit anti-GFP (Invitrogen, A11122) and mouse anti-HA (SIGMA ...
• Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities

  Thomas Kruse, et al., bioRxiv - Biochemistry 2021

  Quote: ... donkey anti-rabbit IgG (Invitrogen), goat anti-rabbit HRP conjugated antibody (WB 1:2000 or 1:5000 ...