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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 7 Benzothiazolol 2 1 1 dimethylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Reactive oxygen species (ROS) were quantitatively and qualitatively determined using the cell permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (ThermoFisher Scientific). H2DCFDA is a type of fluorescein that has been chemically reduced and is used to detect the generation of reactive oxygen intermediates in neutrophils and macrophages ...
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bioRxiv - Cell Biology 2019Quote: 1.5 cm-thick intact human brain slice (Number 7, see Fig. S3) was randomly chosen and passively incubated with 400 ml TO-PRO-3 (T3605, Thermo Fisher, 1:2000 dilution) in PBS at room temperature for 1 week ...
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bioRxiv - Pathology 2022Quote: Cell viability of the MRC-5 cells cultured on LdECM and Ru-LdECM hydrogels was assessed after 1 and 7 days using Calcein AM (Thermo Scientific, Breda, the Netherlands) to stain live cells and propidium iodide (PI ...
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bioRxiv - Physiology 2020Quote: Bone biopsies stored at -80°C between 1 and 7 days were placed into liquid nitrogen contained in a ceramic mortar sprayed with RNaseZap® (ThermoFisher Scientfic, MA, USA). Samples were pulverised using an RNaseZap-treated pestle ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated at 37 °C for 7 days with secondary Alexa Fluor 647 donkey anti-rabbit IgG (1:450; Thermo Fisher Scientific, Rockford, IL) in 3% donkey serum/PTwH and then washed in PTwH for 5 times.
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bioRxiv - Immunology 2020Quote: Subgenomic RT-qPCR was performed on the QuantStudio™ 7 Flex Real-Time PCR System using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with forward primer ...
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bioRxiv - Cell Biology 2023Quote: ... Day 10 post plating (7 days after air lift) cells were fixed in 4% PFA and stained for tight junction protein ZO-1 (Thermo Fisher Cat# 61-7300). Randomized Z stack images were taken at 20x in 3 different places of each transwell for three transwells and imported into ImageJ ...
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bioRxiv - Neuroscience 2022Quote: ... Culture 1 1% (Gibco), fetal bovine serum 2.5% and 2 μg/ml doxycycline hydrochloride.
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bioRxiv - Neuroscience 2021Quote: ... tertiary processing was used to enhance the strength of the punctate labeling in which a biotinylated secondary antibody (1:200) was followed by fluorescently conjugated streptavidin (SA-Alexa488, Invitrogen, 1-2 µg/ml).
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bioRxiv - Pathology 2021Quote: ... and proximal tubular cell line derived from normal kidney (HK-2, ATCC® CRL-2190™) were cultured with 1:1 DMEM/F12 (Gibco 11320-033) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Plasma and saliva IgAs were three-fold diluted (starting from 1:50 and 1:2 dilution, respectively) with infection media (DMEM medium (cat# 11995065, Thermo Fisher Scientific, Waltham, MA) containing 2% fetal bovine serum (FBS)) ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Neuroscience 2019Quote: ... followed by 2 hr incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen] made in PBS-T). Following three additional 10 min washes in PBS-T ...
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bioRxiv - Plant Biology 2022Quote: ... The seedlings were screened by microscopy for YFP and then fixed and processed for immunofluorescence microscopy using a 1:2000 dilution of monoclonal anti-α-tubulin B-5-1-2 antibody (Life Technologies; 32-2500) followed by 1:2000 dilution of Alexa-568 goat anti-mouse antibody (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were incubated for 1 h at 22°C in the presence of a 2:1 mass ratio of TMT10plex reagent (Life Technologies cat. no. 90111) to peptides ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... distal sciatic nerves from WT and Sarm1-/- mice were harvested in 2 mL of a 1:1 solution containing 1XPBS and Wash media (DMEM F-12 + Glutamax (Fisher Scientific, 10-565-042), 10% FBS (Neuromics FBS 002) ...
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bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
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bioRxiv - Molecular Biology 2019Quote: Digested peptides were dissolved in 40 μL of 0.1% formic acid containing 2% (v/v) acetonitrile and 2 μL were injected into an Easy-nLC 1000 system (Thermo Fisher Scientific). Peptides were separated on an Acclaim PepMap™ RSLC column (15 cm × 50 μm inner diameter ...
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bioRxiv - Immunology 2020Quote: ... for 2 days, then passaged 1:2 and cultured overnight in R10 medium (1X Roswell Park Memorial Institute (RPMI, (Life Technologies SAS), 10% fetal calf serum ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded using 7-AAD (7-Aminoactinomycin D, Thermo Fisher Scientific, 00-6993-50). Cells were analyzed using a FACSCanto II flow cytometer (BD ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM magnesium chloride and 1% v/v NP-40) with protease inhibitor cocktail (ThermoFisher). Glycoprotein denaturation of the cell lysate was performed with 1x glycodenaturing buffer (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... Sections were treated for 2 h at RT with secondary goat antibodies (1/400, Invitrogen): anti-rabbit-Al488 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was primed using a 2:1 molar ratio of random hexamer and polydT (Invitrogen) and reverse transcribed with Superscript IV (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from 2×107 BCBL-1 cells using TRIzol™ (ThermoFisher 15596026), followed by DNase I treatment (ThermoFisher AM1907) ...
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bioRxiv - Neuroscience 2020Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Grand Island, NY, USA) for 15 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... beads of four diameters (0.2, 0.5, 1, and 2 μm, Invitrogen by Thermo Fisher Scientific) served as standards ...
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bioRxiv - Evolutionary Biology 2021Quote: ... beads of four diameters (0.2, 0.5, 1, and 2 μm, Invitrogen by Thermo Fisher Scientific) served as standards ...
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bioRxiv - Microbiology 2022Quote: ... Cells were blocked for 1 h in 2 % bovine serum albumin (BSA) (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 2% GlutaMAX (61965-026) and 1% penicillin/streptomycin (15140-148; all from Thermo Fisher Scientific) and kept in a humidified incubator at 37 °C supplied with 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2 ul of a 1:20 dilution of ERCC RNA Spike-in mix (Invitrogen) was added to each sample.
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bioRxiv - Physiology 2019Quote: ... DNA samples and 32P labelled size ladder (2-40kb, 1 kb DNA Extension Ladder Invitrogen) were then loaded into a 0.8% non-denaturing agarose gel ...
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bioRxiv - Plant Biology 2019Quote: ... Purified RNA (2 μg) was incubated with DNase 1 (RNase-Free DNase, Thermo Fisher, USA) at 37 °C for 30 min ...
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bioRxiv - Genomics 2019Quote: ... Single cell suspensions were washed 2 times with DPBS supplemented with 1% Penicillin-Streptomycin (Gibco), 50 µg/ml Gentamicin and 0.25µg/ml amphotericin B and centrifuged at 800 xg for 10 minutes and grown in SAGM supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... HF2597 cells were grown in DMEM/F12 containing 1% N-2 Supplement (Gibco 17502-048), 500 mg/l BSA ...
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bioRxiv - Microbiology 2020Quote: ... washed in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Waltham, MA, USA) two times ...
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bioRxiv - Cell Biology 2019Quote: ... 100 μM of the protein was incubated with 1 mM tris(2-carboxyethyl)phosphine (Invitrogen) for 30 min at RT protected from light ...
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bioRxiv - Bioengineering 2020Quote: ... then incubated in 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Biochemistry 2020Quote: 0.5 mL of 2 µM Lysotracker Deep Red (Thermo Fisher 1 mM stock in DMSO) was added to a 6 cm NGM plate seeded with 0.1 mL of E ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were subsequently incubated for 2 h in secondary antibodies (all 1:500; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with DAPI (4ʹ,6-diamidino-2-phenylindol) 1:10,000 (Thermo Fisher Scientific). Images were recorded using an Axioimager 2 Microscope with cooled CCD camera (SPOT Imaging Solutions ...
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bioRxiv - Neuroscience 2021Quote: ... medium was changed to DMEM/F-12 supplemented with 1× N-2 supplement (Life Technologies), 2 µg/mL heparin ...
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bioRxiv - Cell Biology 2021Quote: ... AlexaFluor-conjugated secondary antibodies (diluted 1:500 in PBS/2%BSA) (LIFE TECHNOLOGIES, Oregon, USA) were added and incubated for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... claudin-5 (1 μg/ml) and occludin (2 μg/ml; all from Thermo Fisher Scientific). After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... at a ratio of 1:2 in Opti-MEM I Reduced Serum Medium (Gibco™). Co-transfections of V5-STX1a and 6xHis-mVAMP2 were adjusted so that equivalent amounts of the two proteins were expressed in culture ...
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bioRxiv - Microbiology 2020Quote: ... Cell nuclei were stained with DAPI (Thermo Scientific, 1:1,000 in 2% BSA in PBS) for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-S100 Protein Ab-2 (S100, 1:500, Thermo Scientific Cat# RB-044-A) was used to identify Schwann cells ...