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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... cDNA prepared thus was diluted 1:60 and 4 µL of the diluted cDNA was used for each qPCR reaction with SYBR green (Invitrogen). β-actin was used as the reference endogenous gene for normalization ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS, and stained using anti-IgG AF647 (1:600 dilution in MACS, 30 min at 4°C) (ThermoFisher). Cells were then fixed using PFA 4% ...
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_10541045) for 3 days at 4°C followed by 2h in 1:800 anti-mouse FluoroNanogold (Life Technologies, A24920) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... then washed three times with PBS and incubated with secondary antibodies (Table 4) and phalloidin (F-actin detection) (1:100) (Invitrogen) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were incubated in the following secondary antibodies diluted to 1:1000 in ovary block overnight at 4°C: goat anti-rabbit IgG Alexa Fluor 633 (Invitrogen), goat anti-mouse IgG Alexa Fluor 488 (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were probed overnight at 4°C using the following primary antibody dilutions: rabbit anti-GFP (1:1000, Invitrogen #A11122), and mouse anti-brp (1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were labeled overnight at 4 °C with Alexa Fluor 488-labeled wheatgerm agglutinin (1:200; W11261, Thermo Fisher) in combination with a rabbit polyclonal primary antiserum ...
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bioRxiv - Microbiology 2021Quote: ... primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, CA, USA) secondary antibodies for 40 min at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... The plate is centrifuged for 1 minute at 1,000 rpm at 4°C and then inserted in the qRT-PCR machine (QuantStudio; Thermofisher scientific) and the run is parametrised ...
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bioRxiv - Cancer Biology 2021Quote: ... ionomycin (1 μg/ml) and monensin (2 μg/ml) for 3-4 hours at 37°C in complete IMDM medium (Gibco). Viability staining was performed using the fixable viability dye eFluor780 ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4°C with gentle agitation in a chicken antiserum against GFP (1:1500, Invitrogen, A11120). After subsequent 3 x 10 min washes in PBST ...
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bioRxiv - Cancer Biology 2021Quote: ... antibodies were used at 1/200 concentration overnight at 4°C and secondary AF488-conjugated anti-mouse Ig (Life Technologies) at 1/500 for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Immunology 2022Quote: ... Doublets and dead cells were excluded based on forward scatter (FSC) and side scatter (SSC) and 4′,6-diamidino-2-phenylindole staining (DAPI, 1 µg/ml; ThermoFisher). All depicted flow cytometry plots were pre-gated on non-debris (by FSC and SSC) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated overnight in a shaker protected from light at 4°C with respective detection reagents (DyLight 488-conjugated streptavidin at 1:400 dilution, Invitrogen #21832 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C the live/dead fixable aqua dead cell marker (1:1,000 in PBS; Life Technologies) before fixation ...
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bioRxiv - Biochemistry 2020Quote: ... in the same buffer at 4 °C overnight followed by incubation with Alexa Fluor 594 anti-mouse IgG Ab (1:1000, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: Samples were run on 4% agarose (Bio-Lab, Israel) in 1× TBE buffer (Biological industries, Israel) supplemented with 5 mM MgCl2 (Ambion), and stained with 0.3 µg/ml ethidium bromide (Sigma-Aldrich) ...
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bioRxiv - Physiology 2020Quote: ... EDL from both legs were dissected and maintained in 4°C Krebs–Henseleit buffer supplemented with 1×MEM amino acid mixture (Invitrogen) and 25mM glucose ...
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bioRxiv - Cell Biology 2020Quote: ... The loading solution contained a 1:10 mixture of 5 mM Fluo-4 AM in dry DMSO and PowerloadTM concentrate (Invitrogen), which was diluted 100-fold into extracellular Tyrode’s solution (NaCl 140mM ...
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bioRxiv - Synthetic Biology 2021Quote: ... Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen). The isolated RNA samples were then stored in −80°C freezer.
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was removed after being centrifuged for 30 min at 20,000 × G and 1 mL of cold (4 °C) 80/20 methanol/water (Optima, LC/MS Grade, Fisher Scientific) was added to the remaining contents ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with primary antibodies in 10% NGS/NDS in PBST over night at 4°C following 1h incubation at 24°C with appropriate Alexa488/568 coupled secondary antibodies (1:1000, Invitrogen). Cell nuclei were stained with DAPI and final images were acquired using Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Neuroscience 2020Quote: ... in 3% NGS and 0.5% Triton-X for 44 h at 4°C and then in goat anti-mouse secondary antibody Alexa 488 (1:500; Invitrogen) in 3% NGS and 0.5% Triton-X for 2 h ...
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bioRxiv - Immunology 2021Quote: ... The RBD probe was prepared at a molar ratio of 4 moles of protein to 1 mole of Alexa Fluor 647-labeled streptavidin (Invitrogen). PBMCs from the five participants were thawed and stained for SARS-CoV-2-specific IgG+ memory B cells ...
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bioRxiv - Pathology 2021Quote: ... The cDNA of the fusion chimera termed ace2 1–618-ddc-abd was then inserted into pcDNA3-4 plasmid (Invitrogen) using custom synthesized complementary primers (IDT ...
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bioRxiv - Systems Biology 2020Quote: ... the pellet resulting from ultracentrifugation of pooled fractions 1-4 was re-suspended in 700 μl of TRIzol (Invitrogen 15596026). Samples were then vortexed for 1 minute ...
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bioRxiv - Biochemistry 2020Quote: ... the media was decanted and cells were incubated for 1 h at 37 °C with Fluo-4 Direct dye (Invitrogen) in FLIPR buffer (1× HBSS ...
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bioRxiv - Genetics 2020Quote: ... Protein extracts were run for 30 min at 200 V on denaturing Bolt 4-12% Bis-Tris Plus Gels in 1× Bolt MOPS SDS buffer (Invitrogen), transferred to a nitrocellulose membrane for 1.5 h at 10 V ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in plating medium (Dulbecco’s modified Eagle’s medium (DMEM)/medium 199 [4:1 (v/v)]) containing 15% (v/v) foetal calf serum (FCS; Life Technologies) and 100 U/ml penicillin and streptomycin ...
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bioRxiv - Neuroscience 2021Quote: ... Primary microglial cells were prepared from post-natal day 2-4 pups as previously described and cultured in DMEM containing 1% PSF (ThermoFisher) 10% heat inactivated FBS (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... and human phosphatidylinositol 4-phosphate 5-kinase type-1 beta (hPIP5KB; Uniprot #O14986) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). The gene encoding yeast Mss4 (Uniprot #P38994 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated with primary antibodies overnight at 4°C using an antibody against V5 (mouse monoclonal R960-25; 1:1000; Invitrogen), spike (mouse monoclonal GTX632604 ...
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bioRxiv - Biochemistry 2021Quote: ... Glycoproteins were resolved by SDS–PAGE (approx. 1×107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred to nitrocellulose membrane (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... For peptide treatment, cells were washed with PBS and treated with peptides (40 μM, 1–4 h) dissolved in OPTI-MEM (Gibco) acidified with 0.15% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos were washed a further three times in PBTw (PBS + 0.1% Tween) and incubated overnight whilst rotating at 4°C in 0.005 U/µl Alexa Fluor 594 phalloidin (Invitrogen, #A12381) in PBTw ...
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bioRxiv - Microbiology 2021Quote: ... the cells were counterstained in 1 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific GmbH, Bremen, Germany) for 10 min at 46°C ...
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bioRxiv - Molecular Biology 2021Quote: ... N2a cells were transfected with pSpCas9(BB)-Puro empty vector or vectors expressing each of the three gRNAs targeting the eIF4E3 exons (meIF4E3 gRNA exon 1, exon 2, exon 4) using Lipofectamine 2000 (Invitrogen). pSpCas9(BB)-Puro was a generous gift from Dr ...
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bioRxiv - Developmental Biology 2021Quote: ... The iPSCs were dissociated (DD0) after 4-5 days of maintenance using 0.25% trypsin / 1 mM EDTA (Thermo Fisher Scientific) at 37°C for 2 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and 8 mM MgSO4 pH 7.0 (2x PHEM buffer) containing 1% Triton X100 for 5 min and then fixed in 4% (v/v) paraformaldehyde (ThermoFisher) in 2x PHEM buffer at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... was reconstituted by submerging the vial at 4°C over night and was diluted 1:5 in ice-cold knock-out DMEM (Cat.-No. 10829, Gibco) until material was evenly dispersed ...
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bioRxiv - Plant Biology 2020Quote: ... Quality and quantity of bulked DNA was checked by running a DNA sample on an agarose gel (1%) and final DNA concentration determined using the Quibit-4 Fluorometer (dsDNA BR assay, Q32850, Thermofisher).
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained in DMEM (25 mM Glucose, 4 mM Glutamine, 1 mM Sodium Pyruvate) supplemented with 10% Fetal Bovine Serum (FBS, Gibco) and 50 units/mL Penicillin/Streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... in blocking buffer overnight in 4°C and stained with secondary AlexaFluor633 donkey anti-goat antibodies (Invitrogen, #A21082; 1:200) for 1 hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Developmental Biology 2023Quote: ... Brains were then washed using 0.5% PBT (4×15 minutes) and incubated 1:500 with secondary antibody (anti-mouse/rabbit Alexa-488/647, Invitrogen/ThermoFisher) for 4 hours at RT before transferring to 4°C for three overnights ...
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bioRxiv - Cell Biology 2022Quote: ... pMDG2.1-VSV-G and p8.91-GAG-POL in a ratio of 4:1:2 to a total of 10 μg of DNA using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions overnight ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 4 hours with Streptavidin AlexaFluor-488 (S32354) and anti-mouse CY5 (A10524) (ThermoFisher Scientific; 1:1,000) where appropriate in a 5% NGS and 0.25% Triton-X in PBS ...